pycom15g11210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003565
Physical Location & Seq
Forward (+)
7574988 .. 7576181
1194 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 396 bp
ATGGCTTATGCTTTGTACTTGGCACCTTCAAATTCCTCGAAAATCACCCCACGACCAGGCAACCATAACACTGCTCAAAAGCTGGTTCGAATAGATAAGCTGCTCCAAAATTCTCCTGCCGCTAGATTTACCAATCTCAAAGCTAAGGCTGAGAAAAGCAATGAAAGCGTAAAACCGAACAGCATGATTTGCCCTGATTGCGATGCAAATGGTGCAGTTCTGTGCTCGCAATGCAAAGGTAGTGGAGTGAACTCTGTTGATCTCTTCGGTGGACGGTTTAAAGCCGGTGACTCGTGCTGGCTCTGCGGTGGTAGAAAGGAGATGTTGTGCGGAAATTGCAACGGAGCTGGGTTTCTCGGAGGTTTCATGAGCACTTGCGATAGTACGCCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

13.81

Weight (kDa)

8.77

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD2_CRD PF25436 60 - 127 4.1e-36 BSD2 cysteine rich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 22
AciI CCGC 3 cut(s) 120, 306, 330
AcsI RAATTY 2 cut(s) 31, 109
AfaI GTAC 2 cut(s) 17, 385
AfiI CCNNNNNNNGG 1 cut(s) 56
AgsI TTSAA 1 cut(s) 30
AjnI CCWGG 1 cut(s) 55
AluBI AGCT 4 cut(s) 82, 100, 143, 347
AluI AGCT 4 cut(s) 82, 100, 143, 347
Alw21I GWGCWC 2 cut(s) 227, 374
ApeKI GCWGC 1 cut(s) 100
ApoI RAATTY 2 cut(s) 31, 109
AsuHPI GGTGA 2 cut(s) 37, 299
AsuII TTCGAA 1 cut(s) 88
BanI GGYRCC 1 cut(s) 22
BauI CACGAG 1 cut(s) 292
Bbv12I GWGCWC 2 cut(s) 227, 374
BbvI GCAGC 1 cut(s) 87
BciT130I CCWGG 1 cut(s) 57
BfaI CTAG 1 cut(s) 123
BisI GCNGC 2 cut(s) 101, 120
BlsI GCNGC 2 cut(s) 102, 121
Bme1390I CCNGG 1 cut(s) 57
BmiI GGNNCC 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 1 cut(s) 193
Bpu10I CCTNAGC 1 cut(s) 144
Bpu14I TTCGAA 1 cut(s) 88
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse118I RCCGGY 1 cut(s) 284
Bse3DI GCAATG 2 cut(s) 166, 236
BseBI CCWGG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMI GCAATG 2 cut(s) 166, 236
BseMII CTCAG 1 cut(s) 141
BseXI GCAGC 1 cut(s) 87
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 234
BshNI GGYRCC 1 cut(s) 22
BsiHKAI GWGCWC 2 cut(s) 227, 374
BsiSI CCGG 1 cut(s) 285
BslI CCNNNNNNNGG 1 cut(s) 56
Bsp119I TTCGAA 1 cut(s) 88
Bsp1286I GDGCHC 2 cut(s) 227, 374
Bsp143I GATC 1 cut(s) 259
BspACI CCGC 3 cut(s) 120, 306, 330
BspCNI CTCAG 1 cut(s) 142
BspHI TCATGA 1 cut(s) 366
BspLI GGNNCC 1 cut(s) 24
BspT104I TTCGAA 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 22
BsrDI GCAATG 2 cut(s) 166, 236
BsrFI RCCGGY 1 cut(s) 284
BssAI RCCGGY 1 cut(s) 284
BssMI GATC 1 cut(s) 259
BssSI CACGAG 1 cut(s) 292
Bst2BI CACGAG 1 cut(s) 292
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 276
Bst6I CTCTTC 1 cut(s) 269
BstAPI GCANNNNNTGC 2 cut(s) 189, 212
BstBI TTCGAA 1 cut(s) 88
BstC8I GCNNGC 2 cut(s) 227, 299
BstDEI CTNAG 2 cut(s) 144, 150
BstKTI GATC 1 cut(s) 262
BstMBI GATC 1 cut(s) 259
BstMWI GCNNNNNNNGC 7 cut(s) 165, 189, 198, 212, 231, 303, 336
BstNI CCWGG 1 cut(s) 57
BstSCI CCNGG 1 cut(s) 55
BstV1I GCAGC 1 cut(s) 87
BtgZI GCGATG 1 cut(s) 216
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 2 cut(s) 227, 299
CciI TCATGA 1 cut(s) 366
Cfr10I RCCGGY 1 cut(s) 284
Csp6I GTAC 2 cut(s) 16, 384
CspCI CAANNNNNGTGG 2 cut(s) 223, 258
CviAII CATG 2 cut(s) 184, 367
CviJI RGCY 8 cut(s) 5, 82, 100, 143, 149, 284, 301, 347
CviKI_1 RGCY 8 cut(s) 5, 82, 100, 143, 149, 284, 301, 347
CviQI GTAC 2 cut(s) 16, 384
DdeI CTNAG 2 cut(s) 144, 150
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
DraI TTTAAA 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 55
FaeI CATG 2 cut(s) 187, 370
FaiI YATR 4 cut(s) 9, 66, 185, 368
FatI CATG 2 cut(s) 183, 366
Fnu4HI GCNGC 2 cut(s) 101, 120
Fsp4HI GCNGC 2 cut(s) 101, 120
FspBI CTAG 1 cut(s) 123
GluI GCNGC 2 cut(s) 101, 120
GsaI CCCAGC 1 cut(s) 351
HapII CCGG 1 cut(s) 285
Hin1II CATG 2 cut(s) 187, 370
HinfI GANTC 1 cut(s) 290
HpaII CCGG 1 cut(s) 285
HphI GGTGA 2 cut(s) 37, 299
Hpy166II GTNNAC 2 cut(s) 250, 272
Hpy188I TCNGA 1 cut(s) 359
Hpy188III TCNNGA 1 cut(s) 367
Hpy8I GTNNAC 2 cut(s) 250, 272
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 4 cut(s) 206, 215, 234, 339
HpyF10VI GCNNNNNNNGC 7 cut(s) 165, 189, 198, 212, 231, 303, 336
HpyF3I CTNAG 2 cut(s) 144, 150
Hsp92II CATG 2 cut(s) 187, 370
Kzo9I GATC 1 cut(s) 259
LmnI GCTCC 2 cut(s) 108, 344
LpnPI CCDG 8 cut(s) 42, 68, 69, 129, 207, 283, 298, 333
Lsp1109I GCAGC 1 cut(s) 87
LweI GCATC 1 cut(s) 193
MaeI CTAG 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 287
MalI GATC 1 cut(s) 261
MboI GATC 1 cut(s) 259
MboII GAAGA 1 cut(s) 256
MhlI GDGCHC 2 cut(s) 227, 374
MluCI AATT 3 cut(s) 31, 109, 334
MlyI GAGTC 1 cut(s) 284
MnlI CCTC 2 cut(s) 46, 353
MseI TTAA 1 cut(s) 279
MspI CCGG 1 cut(s) 285
MspR9I CCNGG 1 cut(s) 57
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 7 cut(s) 165, 189, 198, 212, 231, 303, 336
NdeII GATC 1 cut(s) 259
NlaIII CATG 2 cut(s) 187, 370
NlaIV GGNNCC 1 cut(s) 24
NmuCI GTSAC 1 cut(s) 287
NspV TTCGAA 1 cut(s) 88
PagI TCATGA 1 cut(s) 366
PkrI GCNGC 2 cut(s) 102, 121
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
Psp6I CCWGG 1 cut(s) 55
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 1 cut(s) 55
PspN4I GGNNCC 1 cut(s) 24
RsaI GTAC 2 cut(s) 17, 385
RsaNI GTAC 2 cut(s) 16, 384
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 2 cut(s) 101, 120
Sau3AI GATC 1 cut(s) 259
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 2 cut(s) 227, 374
SetI ASST 7 cut(s) 28, 84, 102, 145, 241, 349, 364
SfaNI GCATC 1 cut(s) 193
SfuI TTCGAA 1 cut(s) 88
Sse9I AATT 3 cut(s) 31, 109, 334
SsiI CCGC 3 cut(s) 120, 306, 330
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 55
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 2 cut(s) 38, 88
TasI AATT 3 cut(s) 31, 109, 334
TatI WGTACW 1 cut(s) 15
TauI GCSGC 1 cut(s) 122
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 1 cut(s) 100
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 2 cut(s) 177, 355
TspGWI ACGGA 1 cut(s) 357
TspRI CASTG 1 cut(s) 76
XapI RAATTY 2 cut(s) 31, 109
XspI CTAG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.