pycom15g13480

eukaryotic translation initiation factor isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003545
Physical Location & Seq
Forward (+)
9176075 .. 9178878
2804 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1251 bp
ATGCAGCAGGGTGATCAACCGGTGTTGAGCTTGAGACCTGGTGGCGGTCGCGGTAGACTCCTTGGTCCTCGCTTCGACTCCTCCACCTCCTCCTCCGCCGCTTCCGGGCCGTCGTCTTCATCATCGTCGTCGTCTTTGGCTTTCGGTTCCTTTTCTTCTGATCTCCCTCTCCTCCGTCCCCATGGCGGCGCTCCCCCCAATTTCTCTATCAAGGCTGGAGATGCACGGTTTGAGGGTCGTGAGCGAGTGAAGTACACCAGAGACCAGATCCTACAGCTTAGAGAGGCTGTTGTGCTCCCTGATGAAATTCTAAAAGTGAAACAAGAAGTTGAAGCTGAATTTTTCAGTGGTGAAGATCAGAGCTGGGGTCGTGCAGAAACAAATCTGCCAGCTCAATCTCCAAGTCCATCTCCAGCTCAAAATCGTTATTCGGAGCCCGACAGCCGGGATTGGCGTGGCCGTTCTGCACCAATTCCTGCCTCTGGAGAGGAACGGTCTTGGGATACTCTTCGGGAGAATAAAGAGTCTGGTAATCGGTTTGATTACAGACAGCAAGATTTAAATCAGGCTCAATTTGCGAGGACACAGAACCAAGGGGGAGGGCCCGCTCCTAATTTAATCAAAGCTGAGGTGCCATGGTCAGCTAGAAGGGGTACTCTATCTGATAAGGACCGTGTGTTGAAAACTGTGAAGGGGATTCTGAACAAGCTGACTCCGGAGAAGTTTGATCTCCTCAAAGGGCAACTTATAGACTCTGGCATTACATCGGCTGATATCTTAAAGGGGGTTATTTCACTGATATTTGATAAGGCCGTGCTAGAACCTACATTTTGCCCCATGTATGCTCTTCTGTGTTCTGATCTTAATGAAAAGCTTCCTCGGTTCCCCTCAGATGAACCAGATGGTAAAGAAATCACTTTCAAGCGCATCCTCTTGAATAACTGCCAGGAGGCTTTTGATGGCACTGACAAATTGAGAGAAGAAGTTAGACAGTTGACTGCCCCCGAACAAGAGATGGAGCGTAGGGATAAAGAAAGATTGGCCAAGATTCGTACTCTGGGAAACATTCGTTTAATTGGAGAGCTTCTGAAGCAGAAGATGGTACCTGAAAAGATTGTACATCACATTGTTGCGGAACTCTTAGGATCTGATAACAAAATTTGTCCTGCTGAGGAAAACGTGGAAGCACTATGTCATTTTTTTACCACAATTGGAAAGCAGCTTGATGAGAAGCCAAAATACGCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05416 map03013 map05416
Pfam Domains
Protein Families
MA3 MIF4G

Protein Analysis

417

Amino Acids

46.23

Weight (kDa)

6.52

Isoelectric Point (pI)

55.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF4G PF02854 227 - 412 4e-44 MIF4G domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 63
Acc65I GGTACC 1 cut(s) 1102
AccB1I GGYRCC 2 cut(s) 631, 1102
AccBSI CCGCTC 1 cut(s) 608
AccI GTMKAC 1 cut(s) 55
AccII CGCG 1 cut(s) 51
AccIII TCCGGA 1 cut(s) 715
AciI CCGC 7 cut(s) 45, 51, 96, 99, 186, 606, 1133
AclWI GGATC 2 cut(s) 262, 1153
AcoI YGGCCR 2 cut(s) 457, 1041
AcsI RAATTY 3 cut(s) 306, 338, 1158
AcuI CTGAAG 1 cut(s) 1109
AfaI GTAC 5 cut(s) 254, 655, 1054, 1104, 1119
AfiI CCNNNNNNNGG 5 cut(s) 44, 105, 185, 444, 482
AgeI ACCGGT 1 cut(s) 19
AgsI TTSAA 4 cut(s) 332, 682, 922, 937
AjnI CCWGG 2 cut(s) 37, 945
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 28, 255
AlwI GGATC 2 cut(s) 262, 1153
Aor13HI TCCGGA 1 cut(s) 715
AoxI GGCC 5 cut(s) 107, 457, 602, 810, 1041
ApaI GGGCCC 1 cut(s) 606
ApeKI GCWGC 2 cut(s) 4, 1219
ApoI RAATTY 3 cut(s) 306, 338, 1158
AsiGI ACCGGT 1 cut(s) 19
Asp700I GAANNNNTTC 1 cut(s) 873
Asp718I GGTACC 1 cut(s) 1102
AspLEI GCGC 2 cut(s) 191, 927
AspS9I GGNCC 5 cut(s) 65, 107, 602, 603, 670
AsuC2I CCSGG 2 cut(s) 106, 446
AsuHPI GGTGA 2 cut(s) 23, 362
AvaII GGWCC 2 cut(s) 65, 670
BaeGI GKGCMC 1 cut(s) 606
BalI TGGCCA 1 cut(s) 1043
BanI GGYRCC 2 cut(s) 631, 1102
BanII GRGCYC 2 cut(s) 438, 606
BarI GAAGNNNNNNTAC 1 cut(s) 1223
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 297
BbvCI CCTCAGC 2 cut(s) 627, 1170
BbvI GCAGC 2 cut(s) 16, 1231
BccI CCATC 5 cut(s) 415, 896, 953, 1009, 1093
BceAI ACGGC 3 cut(s) 94, 444, 797
BciT130I CCWGG 2 cut(s) 39, 947
BciVI GTATCC 1 cut(s) 496
BclI TGATCA 1 cut(s) 13
BcnI CCSGG 2 cut(s) 106, 446
BcoDI GTCTC 2 cut(s) 28, 255
BfaI CTAG 2 cut(s) 645, 818
BfmI CTRYAG 1 cut(s) 272
BfoI RGCGCY 1 cut(s) 192
BfuI GTATCC 1 cut(s) 496
BisI GCNGC 4 cut(s) 5, 99, 187, 1220
BlsI GCNGC 4 cut(s) 6, 100, 188, 1221
Bme1390I CCNGG 4 cut(s) 39, 106, 446, 947
Bme18I GGWCC 2 cut(s) 65, 670
BmgT120I GGNCC 5 cut(s) 65, 107, 602, 603, 670
BmiI GGNNCC 6 cut(s) 148, 435, 604, 633, 884, 1104
BmrFI CCNGG 4 cut(s) 39, 106, 446, 947
BmsI GCATC 2 cut(s) 211, 936
BpiI GAAGAC 1 cut(s) 108
BpmI CTGGAG 3 cut(s) 237, 396, 504
Bpu10I CCTNAGC 2 cut(s) 627, 1170
BpuEI CTTGAG 1 cut(s) 52
BpuMI CCSGG 2 cut(s) 106, 446
BsaBI GATNNNNATC 1 cut(s) 561
BsaI GGTCTC 2 cut(s) 28, 255
BsaJI CCNNGG 5 cut(s) 61, 181, 592, 635, 878
BsaWI WCCGGW 2 cut(s) 19, 715
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 105, 185, 444, 482
Bse118I RCCGGY 1 cut(s) 19
Bse8I GATNNNNATC 1 cut(s) 561
BseAI TCCGGA 1 cut(s) 715
BseBI CCWGG 2 cut(s) 39, 947
BseDI CCNNGG 5 cut(s) 61, 181, 592, 635, 878
BseGI GGATG 1 cut(s) 927
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 5 cut(s) 44, 105, 185, 444, 482
BseMII CTCAG 3 cut(s) 618, 903, 1161
BseRI GAGGAG 5 cut(s) 70, 79, 82, 161, 722
BseSI GKGCMC 1 cut(s) 606
BseXI GCAGC 2 cut(s) 16, 1231
BseYI CCCAGC 1 cut(s) 363
BsgI GTGCAG 2 cut(s) 393, 450
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 5 cut(s) 109, 459, 604, 812, 1043
BshNI GGYRCC 2 cut(s) 631, 1102
BshTI ACCGGT 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 4 cut(s) 20, 105, 445, 716
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 5 cut(s) 44, 105, 185, 444, 482
BsmAI GTCTC 2 cut(s) 28, 255
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 5 cut(s) 109, 459, 604, 812, 1043
Bso31I GGTCTC 2 cut(s) 28, 255
Bsp120I GGGCCC 1 cut(s) 602
Bsp1286I GDGCHC 3 cut(s) 297, 438, 606
Bsp13I TCCGGA 1 cut(s) 715
Bsp1407I TGTACA 1 cut(s) 1117
Bsp143I GATC 7 cut(s) 13, 160, 267, 355, 727, 859, 1145
Bsp19I CCATGG 2 cut(s) 181, 635
BspACI CCGC 7 cut(s) 45, 51, 96, 99, 186, 606, 1133
BspANI GGCC 5 cut(s) 109, 459, 604, 812, 1043
BspCNI CTCAG 3 cut(s) 619, 902, 1162
BspEI TCCGGA 1 cut(s) 715
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 6 cut(s) 148, 435, 604, 633, 884, 1104
BspPI GGATC 2 cut(s) 262, 1153
BspQI GCTCTTC 1 cut(s) 852
BspT107I GGYRCC 2 cut(s) 631, 1102
BspTNI GGTCTC 2 cut(s) 28, 255
BsrBI CCGCTC 1 cut(s) 608
BsrFI RCCGGY 1 cut(s) 19
BsrGI TGTACA 1 cut(s) 1117
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 5 cut(s) 61, 181, 592, 635, 878
BssMI GATC 7 cut(s) 13, 160, 267, 355, 727, 859, 1145
BssT1I CCWWGG 4 cut(s) 61, 181, 592, 635
Bst2UI CCWGG 2 cut(s) 39, 947
Bst4CI ACNGT 5 cut(s) 228, 495, 674, 688, 993
Bst6I CTCTTC 2 cut(s) 513, 852
BstAUI TGTACA 1 cut(s) 1117
BstC8I GCNNGC 2 cut(s) 390, 606
BstDEI CTNAG 5 cut(s) 278, 627, 889, 1141, 1170
BstDSI CCRYGG 2 cut(s) 181, 635
BstF5I GGATG 1 cut(s) 927
BstFNI CGCG 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 2 cut(s) 191, 927
BstKTI GATC 7 cut(s) 16, 163, 270, 358, 730, 862, 1148
BstMAI GTCTC 2 cut(s) 28, 255
BstMBI GATC 7 cut(s) 13, 160, 267, 355, 727, 859, 1145
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 3 cut(s) 221, 575, 1090
BstNI CCWGG 2 cut(s) 39, 947
BstSCI CCNGG 4 cut(s) 37, 104, 444, 945
BstSFI CTRYAG 1 cut(s) 272
BstSLI GKGCMC 1 cut(s) 606
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 16, 1231
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 2 cut(s) 267, 1145
BstYI RGATCY 2 cut(s) 267, 1145
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 5 cut(s) 109, 459, 604, 812, 1043
BtgI CCRYGG 2 cut(s) 181, 635
BtsCI GGATG 1 cut(s) 927
BtsIMutI CAGTG 3 cut(s) 352, 794, 963
Cac8I GCNNGC 2 cut(s) 390, 606
CfoI GCGC 2 cut(s) 191, 927
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 5 cut(s) 65, 107, 602, 603, 670
CsiI ACCWGGT 1 cut(s) 37
Csp6I GTAC 5 cut(s) 253, 654, 1053, 1103, 1118
CspAI ACCGGT 1 cut(s) 19
CviAII CATG 3 cut(s) 182, 636, 838
CviQI GTAC 5 cut(s) 253, 654, 1053, 1103, 1118
DdeI CTNAG 5 cut(s) 278, 627, 889, 1141, 1170
DpnI GATC 7 cut(s) 15, 162, 269, 357, 729, 861, 1147
DpnII GATC 7 cut(s) 13, 160, 267, 355, 727, 859, 1145
DraI TTTAAA 1 cut(s) 561
DrdI GACNNNNNNGTC 1 cut(s) 63
DseDI GACNNNNNNGTC 1 cut(s) 63
EaeI YGGCCR 2 cut(s) 457, 1041
Eam1104I CTCTTC 2 cut(s) 513, 852
EarI CTCTTC 2 cut(s) 513, 852
EciI GGCGGA 1 cut(s) 85
Eco130I CCWWGG 4 cut(s) 61, 181, 592, 635
Eco24I GRGCYC 2 cut(s) 438, 606
Eco31I GGTCTC 2 cut(s) 28, 255
Eco32I GATATC 1 cut(s) 775
Eco47I GGWCC 2 cut(s) 65, 670
Eco57I CTGAAG 1 cut(s) 1109
EcoO109I RGGNCCY 1 cut(s) 602
EcoRII CCWGG 2 cut(s) 37, 945
EcoRV GATATC 1 cut(s) 775
EcoT14I CCWWGG 4 cut(s) 61, 181, 592, 635
EcoT38I GRGCYC 2 cut(s) 438, 606
ErhI CCWWGG 4 cut(s) 61, 181, 592, 635
FaeI CATG 3 cut(s) 185, 639, 841
FaiI YATR 7 cut(s) 183, 637, 749, 839, 843, 1192, 1249
FalI AAGNNNNNCTT 2 cut(s) 729, 761
FaqI GGGAC 1 cut(s) 162
FatI CATG 3 cut(s) 181, 635, 837
FauI CCCGC 1 cut(s) 613
FbaI TGATCA 1 cut(s) 13
FblI GTMKAC 1 cut(s) 55
Fnu4HI GCNGC 4 cut(s) 5, 99, 187, 1220
FokI GGATG 1 cut(s) 914
FriOI GRGCYC 2 cut(s) 438, 606
Fsp4HI GCNGC 4 cut(s) 5, 99, 187, 1220
FspBI CTAG 2 cut(s) 645, 818
GlaI GCGC 2 cut(s) 190, 926
GluI GCNGC 4 cut(s) 5, 99, 187, 1220
GsaI CCCAGC 1 cut(s) 367
GsuI CTGGAG 3 cut(s) 237, 396, 504
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 5 cut(s) 109, 459, 604, 812, 1043
HapII CCGG 4 cut(s) 20, 105, 445, 716
HhaI GCGC 2 cut(s) 191, 927
Hin1II CATG 3 cut(s) 185, 639, 841
Hin6I GCGC 2 cut(s) 189, 925
HinP1I GCGC 2 cut(s) 189, 925
HincII GTYRAC 1 cut(s) 996
HindII GTYRAC 1 cut(s) 996
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 7 cut(s) 57, 77, 524, 697, 712, 752, 1048
HpaII CCGG 4 cut(s) 20, 105, 445, 716
HphI GGTGA 2 cut(s) 23, 362
Hpy166II GTNNAC 3 cut(s) 56, 255, 996
Hpy188I TCNGA 9 cut(s) 160, 360, 433, 664, 702, 859, 892, 1089, 1150
Hpy188III TCNNGA 5 cut(s) 239, 483, 512, 716, 934
Hpy8I GTNNAC 3 cut(s) 56, 255, 996
Hpy99I CGWCG 3 cut(s) 115, 130, 133
HpyAV CCTTC 2 cut(s) 642, 685
HpyCH4III ACNGT 5 cut(s) 228, 495, 674, 688, 993
HpyCH4IV ACGT 1 cut(s) 1179
HpyCH4V TGCA 4 cut(s) 4, 224, 374, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 575, 1090
HpyF3I CTNAG 5 cut(s) 278, 627, 889, 1141, 1170
HpySE526I ACGT 1 cut(s) 1179
Hsp92II CATG 3 cut(s) 185, 639, 841
HspAI GCGC 2 cut(s) 189, 925
Kpn2I TCCGGA 1 cut(s) 715
KpnI GGTACC 1 cut(s) 1106
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 7 cut(s) 13, 160, 267, 355, 727, 859, 1145
LguI GCTCTTC 1 cut(s) 852
LmnI GCTCC 5 cut(s) 196, 300, 433, 613, 1018
Lsp1109I GCAGC 2 cut(s) 16, 1231
LweI GCATC 2 cut(s) 211, 936
MabI ACCWGGT 1 cut(s) 37
MaeI CTAG 2 cut(s) 645, 818
MaeII ACGT 1 cut(s) 1179
MalI GATC 7 cut(s) 15, 162, 269, 357, 729, 861, 1147
MbiI CCGCTC 1 cut(s) 608
MboI GATC 7 cut(s) 13, 160, 267, 355, 727, 859, 1145
MboII GAAGA 7 cut(s) 108, 147, 365, 500, 839, 992, 1108
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 2 cut(s) 267, 1145
MhlI GDGCHC 3 cut(s) 297, 438, 606
MlsI TGGCCA 1 cut(s) 1043
MluNI TGGCCA 1 cut(s) 1043
MlyI GAGTC 5 cut(s) 51, 71, 533, 706, 746
Mox20I TGGCCA 1 cut(s) 1043
MroI TCCGGA 1 cut(s) 715
MroXI GAANNNNTTC 1 cut(s) 873
MscI TGGCCA 1 cut(s) 1043
MseI TTAA 5 cut(s) 560, 617, 779, 864, 1073
Msp20I TGGCCA 1 cut(s) 1043
MspI CCGG 4 cut(s) 20, 105, 445, 716
MspR9I CCNGG 4 cut(s) 39, 106, 446, 947
MunI CAATTG 1 cut(s) 1209
MvaI CCWGG 2 cut(s) 39, 947
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 3 cut(s) 221, 575, 1090
NciI CCSGG 2 cut(s) 106, 446
NcoI CCATGG 2 cut(s) 181, 635
NdeII GATC 7 cut(s) 13, 160, 267, 355, 727, 859, 1145
NlaIII CATG 3 cut(s) 185, 639, 841
NlaIV GGNNCC 6 cut(s) 148, 435, 604, 633, 884, 1104
PciSI GCTCTTC 1 cut(s) 852
PdmI GAANNNNTTC 1 cut(s) 873
PfeI GAWTC 2 cut(s) 697, 1048
PinAI ACCGGT 1 cut(s) 19
PkrI GCNGC 4 cut(s) 6, 100, 188, 1221
PleI GAGTC 5 cut(s) 51, 71, 532, 706, 746
PpsI GAGTC 5 cut(s) 51, 71, 532, 706, 746
Psp6I CCWGG 2 cut(s) 37, 945
PspFI CCCAGC 1 cut(s) 363
PspGI CCWGG 2 cut(s) 37, 945
PspN4I GGNNCC 6 cut(s) 148, 435, 604, 633, 884, 1104
PspOMI GGGCCC 1 cut(s) 602
PspPI GGNCC 5 cut(s) 65, 107, 602, 603, 670
PsuI RGATCY 2 cut(s) 267, 1145
RsaI GTAC 5 cut(s) 254, 655, 1054, 1104, 1119
RsaNI GTAC 5 cut(s) 253, 654, 1053, 1103, 1118
SapI GCTCTTC 1 cut(s) 852
SaqAI TTAA 5 cut(s) 560, 617, 779, 864, 1073
SatI GCNGC 4 cut(s) 5, 99, 187, 1220
Sau3AI GATC 7 cut(s) 13, 160, 267, 355, 727, 859, 1145
Sau96I GGNCC 5 cut(s) 65, 107, 602, 603, 670
SchI GAGTC 5 cut(s) 51, 71, 533, 706, 746
ScrFI CCNGG 4 cut(s) 39, 106, 446, 947
SduI GDGCHC 3 cut(s) 297, 438, 606
SexAI ACCWGGT 1 cut(s) 37
SfaNI GCATC 2 cut(s) 211, 936
SfcI CTRYAG 1 cut(s) 272
SinI GGWCC 2 cut(s) 65, 670
SmiI ATTTAAAT 1 cut(s) 561
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
SsiI CCGC 7 cut(s) 45, 51, 96, 99, 186, 606, 1133
SspMI CTAG 2 cut(s) 645, 818
StyD4I CCNGG 4 cut(s) 37, 104, 444, 945
StyI CCWWGG 4 cut(s) 61, 181, 592, 635
SwaI ATTTAAAT 1 cut(s) 561
TaaI ACNGT 5 cut(s) 228, 495, 674, 688, 993
TaiI ACGT 1 cut(s) 1182
TaqI TCGA 1 cut(s) 75
TatI WGTACW 2 cut(s) 252, 1117
TauI GCSGC 2 cut(s) 101, 189
TfiI GAWTC 2 cut(s) 697, 1048
Tru1I TTAA 5 cut(s) 560, 617, 779, 864, 1073
Tru9I TTAA 5 cut(s) 560, 617, 779, 864, 1073
TscAI CASTG 3 cut(s) 352, 801, 970
TseI GCWGC 2 cut(s) 4, 1219
TspDTI ATGAA 4 cut(s) 108, 318, 882, 909
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 3 cut(s) 352, 801, 970
VpaK11BI GGWCC 2 cut(s) 65, 670
XapI RAATTY 3 cut(s) 306, 338, 1158
XmiI GTMKAC 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 873
XspI CTAG 2 cut(s) 645, 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.