pycom15g16510

Protein of unknown function (DUF502)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008495
Physical Location & Seq
Forward (+)
11575081 .. 11578388
3308 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
ATGGGCGATGAGAAGTCGTCGTCATCGATTGTAATGGCGAGCAGAGACAGAGAACCGCGAGACCGTGAGCTTCTCATTCCCGTGGCGGACTCCGGCGACGAGGATGCATCGTCCAAACCCTCCTCTTCGTCTTCCTCCTCCTCCCACCACACCGGCCGCGAGACGTTTTCCAAAGTTGTTAGAAGTTGGGCATCAAAAAAGTTCATGACAGGATGTGTGATCCTATTTCCAATAGCGATCACTTTCTATATCACATGGTGGTTCATTCACTTTGTGGATGGCTTCTTCTCTCCGATCTATGCTCAACTTGGAATCAACATTTTTGGTCTTGGATTTGTAACTTCCATAACATTCATATTTCTCGTTGGGGTGTTCATGTCATCATGGTTGGGTGCATCTGTCCTGGGCCTTGGTGAGTGGTTTATCAAAAGAATGCCGTTTGTTCGTCATATCTACAATGCCTCCAAGCAGATTAGTTCTGCCATATCCCCAGATCAAAACACACAAGCCTTCAAGGAAGTGGCCATAATAAGGCATCCACGTATTGGTGAATATGCATTCGGGTTCATCACTTCATCCGTTGTCCTACAGAGTTATTCTGGAGATGAGGAGTTGTGCTGTGTTTATGTTCCCACGAACCATCTTTACATTGGTGATGTGTTCCTGGTCAACACCAAGGATGTTATCAGACCAAATTTATCCGTACGGGAGGGAATTGAAATCGTTGTGTCTGGAGGCATGTCAATGCCCCAGATCCTGTCAACGGTTGATTCGCGGATCATGCCAGTGGAAAGATGA

Protein Analysis

266

Amino Acids

29.48

Weight (kDa)

6.3

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF502 PF04367 114 - 214 3.1e-27 Protein of unknown function (DUF502)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 545
AccII CGCG 3 cut(s) 58, 159, 775
AciI CCGC 4 cut(s) 56, 86, 157, 775
AclWI GGATC 3 cut(s) 214, 748, 785
AcoI YGGCCR 2 cut(s) 154, 522
AcsI RAATTY 1 cut(s) 694
AdeI CACNNNGTG 2 cut(s) 258, 274
AfaI GTAC 1 cut(s) 705
AfiI CCNNNNNNNGG 3 cut(s) 531, 545, 763
AgsI TTSAA 2 cut(s) 514, 719
AjnI CCWGG 2 cut(s) 402, 663
AluBI AGCT 1 cut(s) 70
AluI AGCT 1 cut(s) 70
Alw26I GTCTC 3 cut(s) 39, 54, 155
AlwI GGATC 3 cut(s) 214, 748, 785
AlwNI CAGNNNCTG 1 cut(s) 757
AoxI GGCC 3 cut(s) 154, 406, 522
ApoI RAATTY 1 cut(s) 694
AspS9I GGNCC 1 cut(s) 406
AsuHPI GGTGA 3 cut(s) 425, 560, 665
BalI TGGCCA 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 123
BccI CCATC 2 cut(s) 272, 648
BceAI ACGGC 1 cut(s) 421
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 2 cut(s) 404, 665
BcoDI GTCTC 3 cut(s) 39, 54, 155
BfmI CTRYAG 1 cut(s) 587
BisI GCNGC 1 cut(s) 157
BlsI GCNGC 1 cut(s) 158
Bme1390I CCNGG 2 cut(s) 404, 665
BmgT120I GGNCC 1 cut(s) 406
BmrFI CCNGG 2 cut(s) 404, 665
BmsI GCATC 5 cut(s) 94, 116, 200, 404, 544
BpiI GAAGAC 1 cut(s) 123
BpmI CTGGAG 2 cut(s) 621, 753
Bsa29I ATCGAT 1 cut(s) 26
BsaAI YACGTR 1 cut(s) 542
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 4 cut(s) 81, 403, 409, 675
BsaXI ACNNNNNCTCC 2 cut(s) 446, 476
Bsc4I CCNNNNNNNGG 3 cut(s) 531, 545, 763
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 1 cut(s) 785
BseBI CCWGG 2 cut(s) 404, 665
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 4 cut(s) 81, 403, 409, 675
BseGI GGATG 6 cut(s) 109, 218, 283, 535, 575, 685
BseLI CCNNNNNNNGG 3 cut(s) 531, 545, 763
BseNI ACTGG 1 cut(s) 785
BseRI GAGGAG 4 cut(s) 112, 127, 130, 623
BseX3I CGGCCG 1 cut(s) 154
Bsh1236I CGCG 3 cut(s) 58, 159, 775
Bsh1285I CGRYCG 1 cut(s) 157
BshFI GGCC 3 cut(s) 156, 408, 524
BshVI ATCGAT 1 cut(s) 26
BsiEI CGRYCG 1 cut(s) 157
BsiSI CCGG 2 cut(s) 93, 153
BsiWI CGTACG 1 cut(s) 703
BslI CCNNNNNNNGG 3 cut(s) 531, 545, 763
BsmAI GTCTC 3 cut(s) 39, 54, 155
BsmBI CGTCTC 1 cut(s) 155
BsmI GAATGC 2 cut(s) 438, 557
BsnI GGCC 3 cut(s) 156, 408, 524
Bso31I GGTCTC 1 cut(s) 54
Bsp143I GATC 6 cut(s) 219, 237, 294, 493, 753, 777
BspACI CCGC 4 cut(s) 56, 86, 157, 775
BspANI GGCC 3 cut(s) 156, 408, 524
BspDI ATCGAT 1 cut(s) 26
BspFNI CGCG 3 cut(s) 58, 159, 775
BspHI TCATGA 1 cut(s) 204
BspPI GGATC 3 cut(s) 214, 748, 785
BspTNI GGTCTC 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 1 cut(s) 785
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 4 cut(s) 81, 403, 409, 675
BssMI GATC 6 cut(s) 219, 237, 294, 493, 753, 777
BssT1I CCWWGG 2 cut(s) 409, 675
Bst2UI CCWGG 2 cut(s) 404, 665
Bst4CI ACNGT 2 cut(s) 65, 766
Bst6I CTCTTC 1 cut(s) 130
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 1 cut(s) 40
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 6 cut(s) 109, 218, 283, 535, 575, 685
BstFNI CGCG 3 cut(s) 58, 159, 775
BstKTI GATC 6 cut(s) 222, 240, 297, 496, 756, 780
BstMAI GTCTC 3 cut(s) 39, 54, 155
BstMBI GATC 6 cut(s) 219, 237, 294, 493, 753, 777
BstMCI CGRYCG 1 cut(s) 157
BstMWI GCNNNNNNNGC 1 cut(s) 781
BstNI CCWGG 2 cut(s) 404, 665
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 2 cut(s) 402, 663
BstSFI CTRYAG 1 cut(s) 587
BstUI CGCG 3 cut(s) 58, 159, 775
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 753
BstYI RGATCY 1 cut(s) 753
BstZI CGGCCG 1 cut(s) 154
Bsu15I ATCGAT 1 cut(s) 26
BsuRI GGCC 3 cut(s) 156, 408, 524
BsuTUI ATCGAT 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 21
BtsCI GGATG 6 cut(s) 109, 218, 283, 535, 575, 685
BtsIMutI CAGTG 1 cut(s) 792
Cac8I GCNNGC 1 cut(s) 40
CaiI CAGNNNCTG 1 cut(s) 757
CciI TCATGA 1 cut(s) 204
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 406
ClaI ATCGAT 1 cut(s) 26
Csp6I GTAC 1 cut(s) 704
CviAII CATG 6 cut(s) 205, 255, 376, 384, 739, 781
CviJI RGCY 6 cut(s) 70, 156, 282, 408, 509, 524
CviKI_1 RGCY 6 cut(s) 70, 156, 282, 408, 509, 524
CviQI GTAC 1 cut(s) 704
DpnI GATC 6 cut(s) 221, 239, 296, 495, 755, 779
DpnII GATC 6 cut(s) 219, 237, 294, 493, 753, 777
DraIII CACNNNGTG 2 cut(s) 258, 274
EaeI YGGCCR 2 cut(s) 154, 522
EagI CGGCCG 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
EciI GGCGGA 1 cut(s) 101
EclXI CGGCCG 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 409, 675
Eco31I GGTCTC 1 cut(s) 54
Eco52I CGGCCG 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 402, 663
EcoT14I CCWWGG 2 cut(s) 409, 675
EcoT22I ATGCAT 2 cut(s) 109, 559
ErhI CCWWGG 2 cut(s) 409, 675
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 6 cut(s) 208, 258, 379, 387, 742, 784
FatI CATG 6 cut(s) 204, 254, 375, 383, 738, 780
Fnu4HI GCNGC 1 cut(s) 157
FokI GGATG 6 cut(s) 116, 225, 290, 522, 562, 692
Fsp4HI GCNGC 1 cut(s) 157
GluI GCNGC 1 cut(s) 157
GsuI CTGGAG 2 cut(s) 621, 753
HaeIII GGCC 3 cut(s) 156, 408, 524
HapII CCGG 2 cut(s) 93, 153
Hin1II CATG 6 cut(s) 208, 258, 379, 387, 742, 784
HincII GTYRAC 2 cut(s) 670, 762
HindII GTYRAC 2 cut(s) 670, 762
HinfI GANTC 3 cut(s) 89, 312, 770
HpaII CCGG 2 cut(s) 93, 153
HphI GGTGA 3 cut(s) 425, 560, 665
Hpy166II GTNNAC 2 cut(s) 670, 762
Hpy188I TCNGA 2 cut(s) 294, 689
Hpy188III TCNNGA 3 cut(s) 205, 600, 732
Hpy8I GTNNAC 2 cut(s) 670, 762
Hpy99I CGWCG 2 cut(s) 22, 101
HpyAV CCTTC 1 cut(s) 520
HpyCH4III ACNGT 2 cut(s) 65, 766
HpyCH4IV ACGT 2 cut(s) 164, 541
HpyCH4V TGCA 3 cut(s) 107, 395, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 781
HpySE526I ACGT 2 cut(s) 164, 541
Hsp92II CATG 6 cut(s) 208, 258, 379, 387, 742, 784
Kzo9I GATC 6 cut(s) 219, 237, 294, 493, 753, 777
LweI GCATC 5 cut(s) 94, 116, 200, 404, 544
MaeII ACGT 2 cut(s) 164, 541
MaeIII GTNAC 1 cut(s) 337
MalI GATC 6 cut(s) 221, 239, 296, 495, 755, 779
MboI GATC 6 cut(s) 219, 237, 294, 493, 753, 777
MboII GAAGA 3 cut(s) 117, 123, 277
MflI RGATCY 1 cut(s) 753
MlsI TGGCCA 1 cut(s) 524
MluCI AATT 2 cut(s) 694, 714
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 1 cut(s) 83
Mox20I TGGCCA 1 cut(s) 524
Mph1103I ATGCAT 2 cut(s) 109, 559
MscI TGGCCA 1 cut(s) 524
MslI CAYNNNNRTG 3 cut(s) 80, 743, 785
Msp20I TGGCCA 1 cut(s) 524
MspI CCGG 2 cut(s) 93, 153
MspR9I CCNGG 2 cut(s) 404, 665
Mva1269I GAATGC 2 cut(s) 438, 557
MvaI CCWGG 2 cut(s) 404, 665
MvnI CGCG 3 cut(s) 58, 159, 775
MwoI GCNNNNNNNGC 1 cut(s) 781
NdeII GATC 6 cut(s) 219, 237, 294, 493, 753, 777
NlaIII CATG 6 cut(s) 208, 258, 379, 387, 742, 784
NsiI ATGCAT 2 cut(s) 109, 559
NspI RCATGY 1 cut(s) 742
PagI TCATGA 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 2 cut(s) 438, 557
PfeI GAWTC 2 cut(s) 312, 770
Pfl23II CGTACG 1 cut(s) 703
PflMI CCANNNNNTGG 1 cut(s) 545
PkrI GCNGC 1 cut(s) 158
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
Ppu21I YACGTR 1 cut(s) 542
Psp6I CCWGG 2 cut(s) 402, 663
PspGI CCWGG 2 cut(s) 402, 663
PspLI CGTACG 1 cut(s) 703
PspPI GGNCC 1 cut(s) 406
PsrI GAACNNNNNNTAC 2 cut(s) 629, 661
PstNI CAGNNNCTG 1 cut(s) 757
PsuI RGATCY 1 cut(s) 753
RsaI GTAC 1 cut(s) 705
RsaNI GTAC 1 cut(s) 704
RseI CAYNNNNRTG 3 cut(s) 80, 743, 785
SatI GCNGC 1 cut(s) 157
Sau3AI GATC 6 cut(s) 219, 237, 294, 493, 753, 777
Sau96I GGNCC 1 cut(s) 406
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 2 cut(s) 404, 665
SetI ASST 3 cut(s) 72, 167, 544
SfaNI GCATC 5 cut(s) 94, 116, 200, 404, 544
SfcI CTRYAG 1 cut(s) 587
SmiMI CAYNNNNRTG 3 cut(s) 80, 743, 785
Sse9I AATT 2 cut(s) 694, 714
SsiI CCGC 4 cut(s) 56, 86, 157, 775
StyD4I CCNGG 2 cut(s) 402, 663
StyI CCWWGG 2 cut(s) 409, 675
TaaI ACNGT 2 cut(s) 65, 766
TaiI ACGT 2 cut(s) 167, 544
TaqI TCGA 1 cut(s) 26
TasI AATT 2 cut(s) 694, 714
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 2 cut(s) 312, 770
TscAI CASTG 1 cut(s) 792
TspDTI ATGAA 6 cut(s) 193, 253, 343, 364, 556, 564
TspGWI ACGGA 2 cut(s) 568, 691
TspRI CASTG 1 cut(s) 792
Van91I CCANNNNNTGG 1 cut(s) 545
XapI RAATTY 1 cut(s) 694
XceI RCATGY 1 cut(s) 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 647
Zsp2I ATGCAT 2 cut(s) 109, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.