pycom15g22080

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007071
Physical Location & Seq
Forward (+)
16153857 .. 16154879
1023 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGTTTGGGAATGGTGTAACAGCGGGATCGCTGTGTCGTGGTGGGAAGATGCGTAGGGCAGAGTTATTGGGCGGTGCTGTGATCGCGGCGGTGGCGGCGACTCTGCTACTTTTTGGAAACGCCTTCGTTGTGGAGGCTTCGGCTTCGTCTTCGGCGGCTATCGTGCAGAACGCCATCTACTCCAACAAGATTGCCATCTTCTCCAAATCCTATTGCCCGTACGTTCTCTCCCTCAAACTTGGAAAATCGTATTGCTTGCATGCCAAGCGCGTATTTGCTGAACTACATGAGCATCCGTTTGTTGTCGATCTTGATCTTCGAGATGATGGAGCTCAAATTCAGGGTGTTCTTCTAGATGTGGTCGGCCGAACCACTGTCCCGCAGATTTTTGTGAATGGAAAGCACATTGGCGGCTCTGATGACCTCAAAGCAGCGGTTCTGAGTGGCCAGCTTCAGACTCTTCTTAGTATAAGTTGA

Protein Analysis

159

Amino Acids

16.59

Weight (kDa)

8.84

Isoelectric Point (pI)

24.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 84 - 136 5.2e-08 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 86, 270
AciI CCGC 9 cut(s) 23, 72, 86, 89, 95, 155, 380, 411, 434
AclWI GGATC 1 cut(s) 34
AcoI YGGCCR 2 cut(s) 364, 445
AcsI RAATTY 1 cut(s) 336
AcuI CTGAAG 1 cut(s) 437
AfaI GTAC 1 cut(s) 221
AluBI AGCT 2 cut(s) 332, 451
AluI AGCT 2 cut(s) 332, 451
Alw21I GWGCWC 1 cut(s) 334
AlwI GGATC 1 cut(s) 34
AoxI GGCC 2 cut(s) 364, 445
ApeKI GCWGC 1 cut(s) 431
ApoI RAATTY 1 cut(s) 336
AspLEI GCGC 1 cut(s) 270
BalI TGGCCA 1 cut(s) 447
BanII GRGCYC 1 cut(s) 334
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 1 cut(s) 443
BccI CCATC 3 cut(s) 182, 203, 320
BfaI CTAG 1 cut(s) 353
BisI GCNGC 5 cut(s) 87, 96, 156, 412, 432
BlsI GCNGC 5 cut(s) 88, 97, 157, 413, 433
BmsI GCATC 2 cut(s) 39, 301
BpiI GAAGAC 1 cut(s) 141
BsaBI GATNNNNATC 2 cut(s) 194, 312
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bse8I GATNNNNATC 2 cut(s) 194, 312
BseGI GGATG 1 cut(s) 292
BseJI GATNNNNATC 2 cut(s) 194, 312
BseMII CTCAG 1 cut(s) 431
BseX3I CGGCCG 1 cut(s) 364
BseXI GCAGC 1 cut(s) 443
BsgI GTGCAG 1 cut(s) 185
Bsh1236I CGCG 2 cut(s) 86, 270
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 2 cut(s) 366, 447
BsiEI CGRYCG 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 334
BsiWI CGTACG 1 cut(s) 219
BslFI GGGAC 1 cut(s) 362
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 2 cut(s) 366, 447
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 4 cut(s) 26, 81, 307, 313
BspACI CCGC 9 cut(s) 23, 72, 86, 89, 95, 155, 380, 411, 434
BspANI GGCC 2 cut(s) 366, 447
BspCNI CTCAG 1 cut(s) 432
BspFNI CGCG 2 cut(s) 86, 270
BspPI GGATC 1 cut(s) 34
BssMI GATC 4 cut(s) 26, 81, 307, 313
Bst4CI ACNGT 1 cut(s) 376
Bst6I CTCTTC 1 cut(s) 465
BstC8I GCNNGC 3 cut(s) 257, 261, 449
BstDEI CTNAG 2 cut(s) 440, 464
BstF5I GGATG 1 cut(s) 292
BstFNI CGCG 2 cut(s) 86, 270
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 4 cut(s) 29, 84, 310, 316
BstMBI GATC 4 cut(s) 26, 81, 307, 313
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 4 cut(s) 83, 92, 95, 265
BstNSI RCATGY 1 cut(s) 263
BstUI CGCG 2 cut(s) 86, 270
BstV1I GCAGC 1 cut(s) 443
BstV2I GAAGAC 1 cut(s) 141
BstZI CGGCCG 1 cut(s) 364
BsuRI GGCC 2 cut(s) 366, 447
BtsCI GGATG 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 3 cut(s) 257, 261, 449
CfoI GCGC 1 cut(s) 270
Csp6I GTAC 1 cut(s) 220
CviAII CATG 2 cut(s) 260, 287
CviJI RGCY 8 cut(s) 137, 143, 158, 332, 366, 414, 447, 451
CviKI_1 RGCY 8 cut(s) 137, 143, 158, 332, 366, 414, 447, 451
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 2 cut(s) 440, 464
DpnI GATC 4 cut(s) 28, 83, 309, 315
DpnII GATC 4 cut(s) 26, 81, 307, 313
EaeI YGGCCR 2 cut(s) 364, 445
EagI CGGCCG 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
Ecl136II GAGCTC 1 cut(s) 332
EclXI CGGCCG 1 cut(s) 364
Eco24I GRGCYC 1 cut(s) 334
Eco52I CGGCCG 1 cut(s) 364
Eco53kI GAGCTC 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 437
EcoICRI GAGCTC 1 cut(s) 332
EcoT38I GRGCYC 1 cut(s) 334
FaeI CATG 2 cut(s) 263, 290
FaiI YATR 3 cut(s) 261, 288, 470
FaqI GGGAC 1 cut(s) 362
FatI CATG 2 cut(s) 259, 286
FauI CCCGC 2 cut(s) 16, 387
Fnu4HI GCNGC 5 cut(s) 87, 96, 156, 412, 432
FokI GGATG 1 cut(s) 279
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 5 cut(s) 87, 96, 156, 412, 432
FspBI CTAG 1 cut(s) 353
GlaI GCGC 1 cut(s) 269
GluI GCNGC 5 cut(s) 87, 96, 156, 412, 432
HaeIII GGCC 2 cut(s) 366, 447
HhaI GCGC 1 cut(s) 270
Hin1II CATG 2 cut(s) 263, 290
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HinfI GANTC 2 cut(s) 100, 457
Hpy188I TCNGA 3 cut(s) 418, 441, 456
Hpy188III TCNNGA 3 cut(s) 311, 320, 353
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 2 cut(s) 166, 259
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 92, 95, 265
HpyF3I CTNAG 2 cut(s) 440, 464
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 2 cut(s) 263, 290
HspAI GCGC 1 cut(s) 268
Kzo9I GATC 4 cut(s) 26, 81, 307, 313
LmnI GCTCC 1 cut(s) 329
LpnPI CCDG 2 cut(s) 326, 461
Lsp1109I GCAGC 1 cut(s) 443
LweI GCATC 2 cut(s) 39, 301
MaeI CTAG 1 cut(s) 353
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 16
MalI GATC 4 cut(s) 28, 83, 309, 315
MboI GATC 4 cut(s) 26, 81, 307, 313
MboII GAAGA 6 cut(s) 58, 141, 190, 308, 341, 452
MhlI GDGCHC 1 cut(s) 334
MlsI TGGCCA 1 cut(s) 447
MluCI AATT 1 cut(s) 336
MluNI TGGCCA 1 cut(s) 447
MlyI GAGTC 2 cut(s) 94, 451
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 3 cut(s) 127, 242, 434
Mox20I TGGCCA 1 cut(s) 447
MscI TGGCCA 1 cut(s) 447
Msp20I TGGCCA 1 cut(s) 447
MspA1I CMGCKG 2 cut(s) 23, 434
MvnI CGCG 2 cut(s) 86, 270
MwoI GCNNNNNNNGC 4 cut(s) 83, 92, 95, 265
NdeII GATC 4 cut(s) 26, 81, 307, 313
NlaIII CATG 2 cut(s) 263, 290
NspI RCATGY 1 cut(s) 263
PaeI GCATGC 1 cut(s) 263
Pfl23II CGTACG 1 cut(s) 219
PkrI GCNGC 5 cut(s) 88, 97, 157, 413, 433
PleI GAGTC 2 cut(s) 94, 451
PpsI GAGTC 2 cut(s) 94, 451
Psp124BI GAGCTC 1 cut(s) 334
PspLI CGTACG 1 cut(s) 219
PsrI GAACNNNNNNTAC 2 cut(s) 161, 193
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SacI GAGCTC 1 cut(s) 334
SatI GCNGC 5 cut(s) 87, 96, 156, 412, 432
Sau3AI GATC 4 cut(s) 26, 81, 307, 313
SchI GAGTC 2 cut(s) 94, 451
SduI GDGCHC 1 cut(s) 334
SetI ASST 4 cut(s) 225, 334, 426, 453
SfaNI GCATC 2 cut(s) 39, 301
SphI GCATGC 1 cut(s) 263
Sse9I AATT 1 cut(s) 336
SsiI CCGC 9 cut(s) 23, 72, 86, 89, 95, 155, 380, 411, 434
SspMI CTAG 1 cut(s) 353
SstI GAGCTC 1 cut(s) 334
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 1 cut(s) 225
TaqI TCGA 2 cut(s) 306, 319
TasI AATT 1 cut(s) 336
TauI GCSGC 4 cut(s) 89, 98, 158, 414
TscAI CASTG 1 cut(s) 379
TseI GCWGC 1 cut(s) 431
TspGWI ACGGA 1 cut(s) 285
TspRI CASTG 1 cut(s) 379
XapI RAATTY 1 cut(s) 336
XbaI TCTAGA 1 cut(s) 352
XceI RCATGY 1 cut(s) 263
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.