RchiOBHm_Chr2g0100301

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007071
Physical Location & Seq
Reverse (-)
12304525 .. 12306292
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47495

Sequence Viewer

Length: 414 bp
ATGGAATGTCGTAACAGCTCGATCGGTGAGGATATGGGGCGGTCGGAGTTTGTGATCGTCACGGTGGCGGCGGTTCTGCTGTTGTTGGGAAACGACGTCGTTGAGGCTTCGAGTTCACCATCGGCGTTTGTGCAGAACGTCATCTACTCCAACAAGATTGCCATCTTCTCCAAATCGTATTGCCCGTATTGCTTGCGTGCCAAGCGCATATTCAGTGAATTACATGAGCAACCTTATGTTGTAGAACTTGATCTTCGAGATGATGGGGGGCAAATTCAGAGTGTTCTTCTAGATGTGGTAGGCCGATCCACTGTCCCACAAGTATTTGTGAATGGCAAGCACATCGGTGGTTCTGATGATCTCAAAGCCGCTGTTGCGAGTGGCCAACTTCAGAAGCTTCTTAGCATAAGTTGA

Protein Analysis

137

Amino Acids

14.82

Weight (kDa)

5.28

Isoelectric Point (pI)

52.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 53 - 115 5.8e-17 Glutaredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
AciI CCGC 4 cut(s) 40, 68, 71, 369
AclWI GGATC 1 cut(s) 300
AcoI YGGCCR 1 cut(s) 382
AcsI RAATTY 1 cut(s) 273
AcuI CTGAAG 1 cut(s) 374
AcyI GRCGYC 1 cut(s) 96
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AleI CACNNNNGTG 1 cut(s) 345
AluBI AGCT 2 cut(s) 18, 397
AluI AGCT 2 cut(s) 18, 397
AlwI GGATC 1 cut(s) 300
AoxI GGCC 2 cut(s) 301, 382
ApoI RAATTY 1 cut(s) 273
AspLEI GCGC 1 cut(s) 207
AsuHPI GGTGA 2 cut(s) 38, 108
BalI TGGCCA 1 cut(s) 384
BccI CCATC 3 cut(s) 127, 170, 257
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BfaI CTAG 1 cut(s) 290
BisI GCNGC 2 cut(s) 69, 369
BlsI GCNGC 2 cut(s) 70, 370
BsaBI GATNNNNATC 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 96
Bse8I GATNNNNATC 1 cut(s) 161
BseJI GATNNNNATC 1 cut(s) 161
BsgI GTGCAG 1 cut(s) 152
Bsh1285I CGRYCG 2 cut(s) 24, 44
BshFI GGCC 2 cut(s) 303, 384
BsiEI CGRYCG 2 cut(s) 24, 44
BslFI GGGAC 1 cut(s) 299
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 2 cut(s) 303, 384
Bsp143I GATC 5 cut(s) 21, 54, 250, 305, 358
BspACI CCGC 4 cut(s) 40, 68, 71, 369
BspANI GGCC 2 cut(s) 303, 384
BspPI GGATC 1 cut(s) 300
BssMI GATC 5 cut(s) 21, 54, 250, 305, 358
BssNI GRCGYC 1 cut(s) 96
Bst4CI ACNGT 2 cut(s) 64, 313
BstACI GRCGYC 1 cut(s) 96
BstC8I GCNNGC 3 cut(s) 194, 198, 338
BstDEI CTNAG 1 cut(s) 401
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 5 cut(s) 24, 57, 253, 308, 361
BstMBI GATC 5 cut(s) 21, 54, 250, 305, 358
BstMCI CGRYCG 2 cut(s) 24, 44
BstMWI GCNNNNNNNGC 3 cut(s) 189, 202, 374
BsuRI GGCC 2 cut(s) 303, 384
BtsIMutI CAGTG 2 cut(s) 220, 309
Cac8I GCNNGC 3 cut(s) 194, 198, 338
CfoI GCGC 1 cut(s) 207
CviAII CATG 1 cut(s) 224
CviJI RGCY 6 cut(s) 18, 107, 303, 368, 384, 397
CviKI_1 RGCY 6 cut(s) 18, 107, 303, 368, 384, 397
DdeI CTNAG 1 cut(s) 401
DpnI GATC 5 cut(s) 23, 56, 252, 307, 360
DpnII GATC 5 cut(s) 21, 54, 250, 305, 358
EaeI YGGCCR 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 374
FaeI CATG 1 cut(s) 227
FaiI YATR 5 cut(s) 35, 209, 225, 237, 407
FaqI GGGAC 1 cut(s) 299
FatI CATG 1 cut(s) 223
Fnu4HI GCNGC 2 cut(s) 69, 369
Fsp4HI GCNGC 2 cut(s) 69, 369
FspBI CTAG 1 cut(s) 290
GlaI GCGC 1 cut(s) 206
GluI GCNGC 2 cut(s) 69, 369
HaeIII GGCC 2 cut(s) 303, 384
HhaI GCGC 1 cut(s) 207
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 1 cut(s) 227
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 395
HphI GGTGA 2 cut(s) 38, 108
Hpy166II GTNNAC 1 cut(s) 116
Hpy188I TCNGA 4 cut(s) 46, 279, 355, 393
Hpy188III TCNNGA 2 cut(s) 257, 290
Hpy8I GTNNAC 1 cut(s) 116
Hpy99I CGWCG 2 cut(s) 98, 101
HpyCH4III ACNGT 2 cut(s) 64, 313
HpyCH4IV ACGT 2 cut(s) 96, 138
HpyCH4V TGCA 1 cut(s) 133
HpyF10VI GCNNNNNNNGC 3 cut(s) 189, 202, 374
HpyF3I CTNAG 1 cut(s) 401
HpySE526I ACGT 2 cut(s) 96, 138
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 1 cut(s) 227
HspAI GCGC 1 cut(s) 205
Kzo9I GATC 5 cut(s) 21, 54, 250, 305, 358
MaeI CTAG 1 cut(s) 290
MaeII ACGT 2 cut(s) 96, 138
MaeIII GTNAC 2 cut(s) 11, 58
MalI GATC 5 cut(s) 23, 56, 252, 307, 360
MboI GATC 5 cut(s) 21, 54, 250, 305, 358
MboII GAAGA 3 cut(s) 157, 245, 278
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 2 cut(s) 218, 273
MluNI TGGCCA 1 cut(s) 384
MmeI TCCRAC 2 cut(s) 24, 174
MnlI CCTC 2 cut(s) 22, 97
Mox20I TGGCCA 1 cut(s) 384
MscI TGGCCA 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 345
Msp20I TGGCCA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 371
MwoI GCNNNNNNNGC 3 cut(s) 189, 202, 374
NdeII GATC 5 cut(s) 21, 54, 250, 305, 358
NlaIII CATG 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 58
OliI CACNNNNGTG 1 cut(s) 345
PcsI WCGNNNNNNNCGW 1 cut(s) 182
PkrI GCNGC 2 cut(s) 70, 370
Ple19I CGATCG 1 cut(s) 24
PsrI GAACNNNNNNTAC 2 cut(s) 128, 160
PvuI CGATCG 1 cut(s) 24
RseI CAYNNNNRTG 1 cut(s) 345
SatI GCNGC 2 cut(s) 69, 369
Sau3AI GATC 5 cut(s) 21, 54, 250, 305, 358
SetI ASST 5 cut(s) 20, 99, 141, 235, 399
SmiMI CAYNNNNRTG 1 cut(s) 345
Sse9I AATT 2 cut(s) 218, 273
SsiI CCGC 4 cut(s) 40, 68, 71, 369
SspMI CTAG 1 cut(s) 290
TaaI ACNGT 2 cut(s) 64, 313
TaiI ACGT 2 cut(s) 99, 141
TaqI TCGA 3 cut(s) 20, 110, 256
TasI AATT 2 cut(s) 218, 273
TauI GCSGC 2 cut(s) 71, 371
TscAI CASTG 2 cut(s) 220, 316
TseFI GTSAC 1 cut(s) 58
Tsp45I GTSAC 1 cut(s) 58
TspRI CASTG 2 cut(s) 220, 316
XapI RAATTY 1 cut(s) 273
XbaI TCTAGA 1 cut(s) 289
XspI CTAG 1 cut(s) 290
ZraI GACGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.