pycom15g22730

Belongs to the glucose-6-phosphate 1-epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008798
Physical Location & Seq
Forward (+)
16803401 .. 16804759
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGCTCGTTTCGCTCCCCACCCTCACTTCTCCGAACCCCCGCCGAGTCAACCGGTTTTGGCAAACAACGCCAAAAACGTCGTCGTCCGTGATGGCTGTTGCAAGCGGCCCCGCCAGCAAAGAGCTCGCCACTGCGGGGGTGAAATTTGCAGAGGGAGAAGAGAAATTGCCAAAGGTTGTTTTGACCAGCGCCGGCGGCAGTGAGGCAGATGTATATTTATATGGTGGTGTTATTACTTCATGGAAAGTCCCAAATGGGAGAGACCTCCTTTTTATTAGACCTGATGCGGTGTTCAACAAGAAGAAACCAATCAGTGGAGGCGTTCCACATTGTTTTCCACAGTTTGGGCCAGGCCCTATGCAGCAGCACGGATTTGCAAGAAATCTGGACTGGTCCATCATTGATTCTGAAAATGTGCAAGGATCGATCCTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko01100 ko01110 ko01120 ko01130 map00010 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
Aldose_epim

Protein Analysis

146

Amino Acids

15.58

Weight (kDa)

9.41

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 58 - 138 2.5e-18 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 314, 344
AciI CCGC 6 cut(s) 40, 105, 111, 134, 195, 287
AclWI GGATC 2 cut(s) 421, 430
AcsI RAATTY 1 cut(s) 143
AfiI CCNNNNNNNGG 3 cut(s) 135, 314, 344
AgeI ACCGGT 1 cut(s) 51
AgsI TTSAA 1 cut(s) 295
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 1 cut(s) 124
AluI AGCT 1 cut(s) 124
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 2 cut(s) 421, 430
AoxI GGCC 3 cut(s) 106, 347, 352
ApeKI GCWGC 2 cut(s) 361, 364
ApoI RAATTY 1 cut(s) 143
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AsiGI ACCGGT 1 cut(s) 51
AspLEI GCGC 1 cut(s) 191
AspS9I GGNCC 4 cut(s) 107, 347, 353, 393
AsuHPI GGTGA 1 cut(s) 151
AvaII GGWCC 1 cut(s) 393
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 373, 376
BccI CCATC 2 cut(s) 85, 404
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 1 cut(s) 351
BcoDI GTCTC 1 cut(s) 255
BfoI RGCGCY 1 cut(s) 192
BisI GCNGC 4 cut(s) 106, 196, 362, 365
BlsI GCNGC 4 cut(s) 107, 197, 363, 366
Bme1390I CCNGG 1 cut(s) 351
Bme18I GGWCC 1 cut(s) 393
BmgT120I GGNCC 4 cut(s) 107, 347, 353, 393
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 1 cut(s) 351
BmsI GCATC 1 cut(s) 274
Bsa29I ATCGAT 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 255
BsaWI WCCGGW 1 cut(s) 51
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 314, 344
Bse118I RCCGGY 2 cut(s) 51, 191
Bse1I ACTGG 1 cut(s) 395
BseBI CCWGG 1 cut(s) 351
BseCI ATCGAT 1 cut(s) 425
BseLI CCNNNNNNNGG 3 cut(s) 135, 314, 344
BseNI ACTGG 1 cut(s) 395
BseXI GCAGC 2 cut(s) 373, 376
BshFI GGCC 3 cut(s) 108, 349, 354
BshTI ACCGGT 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 2 cut(s) 52, 192
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 3 cut(s) 135, 314, 344
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 3 cut(s) 108, 349, 354
Bso31I GGTCTC 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 2 cut(s) 422, 426
BspACI CCGC 6 cut(s) 40, 105, 111, 134, 195, 287
BspANI GGCC 3 cut(s) 108, 349, 354
BspDI ATCGAT 1 cut(s) 425
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 2 cut(s) 421, 430
BspTNI GGTCTC 1 cut(s) 255
BsrFI RCCGGY 2 cut(s) 51, 191
BsrI ACTGG 1 cut(s) 395
BssAI RCCGGY 2 cut(s) 51, 191
BssMI GATC 2 cut(s) 422, 426
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 342
Bst6I CTCTTC 1 cut(s) 153
BstC8I GCNNGC 4 cut(s) 103, 115, 126, 193
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 1 cut(s) 191
BstKTI GATC 2 cut(s) 425, 429
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 2 cut(s) 422, 426
BstMWI GCNNNNNNNGC 4 cut(s) 10, 67, 114, 195
BstNI CCWGG 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 349
BstV1I GCAGC 2 cut(s) 373, 376
Bsu15I ATCGAT 1 cut(s) 425
BsuRI GGCC 3 cut(s) 108, 349, 354
BsuTUI ATCGAT 1 cut(s) 425
BtsI GCAGTG 2 cut(s) 129, 205
BtsIMutI CAGTG 3 cut(s) 129, 205, 319
Cac8I GCNNGC 4 cut(s) 103, 115, 126, 193
CfoI GCGC 1 cut(s) 191
Cfr10I RCCGGY 2 cut(s) 51, 191
Cfr13I GGNCC 4 cut(s) 107, 347, 353, 393
ClaI ATCGAT 1 cut(s) 425
CspAI ACCGGT 1 cut(s) 51
CviAII CATG 1 cut(s) 240
CviJI RGCY 5 cut(s) 95, 108, 124, 349, 354
CviKI_1 RGCY 5 cut(s) 95, 108, 124, 349, 354
DpnI GATC 2 cut(s) 424, 428
DpnII GATC 2 cut(s) 422, 426
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 393
Eco53kI GAGCTC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 349
EcoT38I GRGCYC 1 cut(s) 126
FaeI CATG 1 cut(s) 243
FaiI YATR 6 cut(s) 214, 220, 222, 241, 359, 436
FaqI GGGAC 1 cut(s) 233
FatI CATG 1 cut(s) 239
FauI CCCGC 3 cut(s) 47, 118, 127
Fnu4HI GCNGC 4 cut(s) 106, 196, 362, 365
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 4 cut(s) 106, 196, 362, 365
GlaI GCGC 1 cut(s) 190
GluI GCNGC 4 cut(s) 106, 196, 362, 365
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 3 cut(s) 108, 349, 354
HapII CCGG 2 cut(s) 52, 192
HhaI GCGC 1 cut(s) 191
Hin1II CATG 1 cut(s) 243
Hin6I GCGC 1 cut(s) 189
HinP1I GCGC 1 cut(s) 189
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 2 cut(s) 45, 404
HpaII CCGG 2 cut(s) 52, 192
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 2 cut(s) 33, 409
Hpy188III TCNNGA 1 cut(s) 386
Hpy8I GTNNAC 1 cut(s) 49
Hpy99I CGWCG 2 cut(s) 82, 85
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 1 cut(s) 77
HpyCH4V TGCA 5 cut(s) 101, 149, 361, 377, 418
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 67, 114, 195
HpySE526I ACGT 1 cut(s) 77
Hsp92II CATG 1 cut(s) 243
HspAI GCGC 1 cut(s) 189
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
Kzo9I GATC 2 cut(s) 422, 426
LmnI GCTCC 1 cut(s) 18
LpnPI CCDG 9 cut(s) 65, 127, 199, 205, 294, 336, 363, 371, 376
Lsp1109I GCAGC 2 cut(s) 373, 376
LweI GCATC 1 cut(s) 274
MaeII ACGT 1 cut(s) 77
MalI GATC 2 cut(s) 424, 428
MboI GATC 2 cut(s) 422, 426
MboII GAAGA 2 cut(s) 170, 313
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 2 cut(s) 143, 164
MlyI GAGTC 1 cut(s) 54
MnlI CCTC 5 cut(s) 32, 145, 196, 275, 311
MreI CGCCGGCG 1 cut(s) 191
MroNI GCCGGC 1 cut(s) 191
MspI CCGG 2 cut(s) 52, 192
MspR9I CCNGG 1 cut(s) 351
MvaI CCWGG 1 cut(s) 351
MwoI GCNNNNNNNGC 4 cut(s) 10, 67, 114, 195
NaeI GCCGGC 1 cut(s) 193
NdeII GATC 2 cut(s) 422, 426
NgoMIV GCCGGC 1 cut(s) 191
NlaIII CATG 1 cut(s) 243
NlaIV GGNNCC 1 cut(s) 109
NmeAIII GCCGAG 1 cut(s) 68
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PdiI GCCGGC 1 cut(s) 193
PfeI GAWTC 1 cut(s) 404
PflMI CCANNNNNTGG 2 cut(s) 314, 344
PinAI ACCGGT 1 cut(s) 51
PkrI GCNGC 4 cut(s) 107, 197, 363, 366
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 349
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 4 cut(s) 107, 347, 353, 393
SacI GAGCTC 1 cut(s) 126
SatI GCNGC 4 cut(s) 106, 196, 362, 365
Sau3AI GATC 2 cut(s) 422, 426
Sau96I GGNCC 4 cut(s) 107, 347, 353, 393
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 1 cut(s) 351
SduI GDGCHC 1 cut(s) 126
SetI ASST 5 cut(s) 80, 126, 177, 267, 283
SfaNI GCATC 1 cut(s) 274
SgrAI CRCCGGYG 1 cut(s) 191
SinI GGWCC 1 cut(s) 393
Sse9I AATT 2 cut(s) 143, 164
SsiI CCGC 6 cut(s) 40, 105, 111, 134, 195, 287
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 349
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 1 cut(s) 80
TaqI TCGA 1 cut(s) 425
TasI AATT 2 cut(s) 143, 164
TauI GCSGC 2 cut(s) 108, 198
TfiI GAWTC 1 cut(s) 404
TscAI CASTG 3 cut(s) 136, 205, 319
TseI GCWGC 2 cut(s) 361, 364
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 2 cut(s) 76, 384
TspRI CASTG 3 cut(s) 136, 205, 319
Van91I CCANNNNNTGG 2 cut(s) 314, 344
VpaK11BI GGWCC 1 cut(s) 393
XapI RAATTY 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.