pycom15g25510

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003836
Physical Location & Seq
Forward (+)
20010285 .. 20014084
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2676 bp
ATGGCCGTTATTATTTTTGAAATGTATTATATGTTTGCTCTTCTATTACTTTTCAAGACTTGGTTTCTATTCCTTTCTTCTGCAGAGAGACCATTATACTTATTATATGTATTCCTTTCTTCTGCTGAGAGACTATTATACTTATTATATGTTGATGCAGGTGTGATGGAGATATCATTGTTCAAAGCACTTCTTAATAACATCTCCTCTTTTTTCCATTTATCATCTCATAACAACATAAACTTTGACCCGGTTCTGAAGTACTACAAGAGCGCCGAAGAGATATTAAAACTGTTGAAGACTCTACTTGATGCCATTGCTGATTCTGAAATAGCTTCTTATGAAATACTTGATAAGCCATTTGAGGAACTGGGTCAGTATGTTGATGAGTTGAGGGAGCAAATTGAAGACTGGCAGCCATTGTCTAGTAAAGTCAATCTTATTCTTCAAGTTGAATCGTTGATATCAAAGATTTGGGCTTCTGGCCTGGATATCTTTCAACTGTTGAAAACTTCCCCCCAGCATCTTCCTGATGAAATGGGTTCAGCATCTCTTGAGCAATGCATACAAAAACTTGAGCACATGGGCTATGAACAAATGTCAACTGTCATTAAGGATGCTATAAGCGATCAAGTGGAGGGTGTTGGACCAAGCTCAGAGATCCTAGTGAAAATTGCAGAGGGCCTTAGCTTGAAGTCAAACCAGGAGATTCTTATTGAGGCTGTAGCACTTGAAAAGTTGAAGGAAAATGCTGAACAATCTGAAAAGATTGAGGAAGCGGAGTATATTGAGCAAATGATATCCCTTGTTACCCGTATGCACGAGCGCCTTATTACCATAAAGCAAGCCAGGAGCTGCAGCCCTGTTCCAATACCTGCTGATTTCTGTTGCCCCCTCTCTCTGGAGCTGATGACGGATCCAGTGATTGTGGCATCAGGACAAACCTATGAGAGAACTTTCATCAAGAACTGGATCGGTCTTGGCCTCACTGTTTGTCCAAAGACGCGGCAGACTTTGGCTCACACCAATTTGATACCTAATTACACTGTAAAGGCACTAATTGCAAATTGGTGTGAGTCAAACAATGTGAAACTGCCTGATCCGGTAAAGTCTGTTGGCTTAAATAAGTCAGCACACCTTCTTGGGCATGCAGAGCCTGATACACCAAAGGATTCTCCTATTTTTTCTCATTCCAGTGTCAATGAATCAATGTCACCTGAGTCTACTCGGTCTACTGGTTTGCCCTCTAAGAACATGTTTTCATCTGGTCCACTGCATCGGGAGAGAATTTCTCCTTTGCATCCTCGTTCAACATCAGAAGGTTCCTTGCCTTGTATAGTTGGAAATGGGCAGGCTTTGGATATTGAAAGAATATCACTAGCAAATTCTGAAGACAGGTCTGCCAACTTAGAAGAAAGGAGTACAGATCTAGATAGCCAGCACTCTATGTCGCCATCTCGAGATGAACTTCCTATTTCTATTGAAGATGAGCAGCCATCTCAAAGCCATAATAGAACTGCGTCAGCTTCTAGCGTACTTTTTAATGCCAATGGTACTCAAGGAACTTCTGTCAATGCCAATGGGGTCTTACAGGTATCGACGAATCTGTCAGGCGACAGCAGTGACGCTTCTGGGGAGTTGAAATCACAAGCACAAACTGTTGCTCCCTTGGCCCCCCAACTGAGAGAAACTGAGTCACCAACCAGAATGGCAGAGTCAAGACCTCGAAACCAAATGTGGCGCCGACCATCTAGCTTCCCGAGGATAGTTTCTTCTCCACCTATTGAAACAAGGCCTGATCGTTCTGAACTTGAAGCCCAAGTTCGTAACCTAGTTGATGATTTGAAAAGCACTTCACTTGATACACAAAGAGAGGCAACATTTAAACTCCGGCTACTTGCCAAACATAATATGGATAATCGGATTGTTATTGCAAATTGTGGGGCCATTGGCTTGTTGGTGGATTTGCTCCGCTCTACAGATACAAGAGTTCAAGAGAATGCTGTCACAGCACTTCTTAACTTATCGATTAATGATAACAACAAAACTGCAATTGCCACTGCCAATGCAATTGAACCTCTGATTCATGTCCTTGAGACAGGGAGTGCTGAGGCCAAGGAGAACTCAGCTGCCACTCTATTTAGCCTTTCGGTGATCGAGGACAACAAGGTTCGTATTGGAAGGTCAGGGGCTATTGGGCCTCTGGTTGATTTATTGGGGAATGGGAGTCCCAGAGGGAGGAAAGATGCAGCCACAGCTTTGTTTAATTTGTCAATTTTTCATGAGAACAAGGATAGAATTGTTCAAGCTGGTGCTGTAAAGTACCTAGTGGAGTTGATGGACCCTGCGGCGGGAATGGTTGACAAGGCAGTTGCTGTTTTGGCTAATCTTTCGACAATTCCCGAGGGAAGGACAGCAATTGGTCAGGAGGGTGGGATCCCTGTTCTTGTCGAGGTTGTTGAGTTGGGTTCTGCAAGAGGGAAGGAGAACGCAGCTGCTGCTCTTTTACAGCTCTGCACAAACAGTAACAGATTTTGCAGTCAGGTCCTCCAAGAAGGAGCTGTCCCACCATTAGTGGTATTGTCACAATCCGGCACCCCTAGGGCAAAAGAAAAGGCACAGACACTCCTTAGCTACTTCAGAAACCATAGACAAGGTAATGCTGGTAGAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

892

Amino Acids

97.66

Weight (kDa)

5.33

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB2_N PF25240 57 - 196 3.8e-59 PUB2-like, N-terminal domain
U-box PF04564 291 - 361 1.8e-21 U-box domain
ARM_PUB PF25598 607 - 883 8.4e-30 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 608 - 780 6.3e-09 ARMC5
Arm PF00514 639 - 676 2.3e-10 Armadillo/beta-catenin-like repeat
Arm PF00514 721 - 758 9.5e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 751 - 878 1.1e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 149
AasI GACNNNNNNGTC 1 cut(s) 1606
Acc36I ACCTGC 2 cut(s) 149, 883
AccB1I GGYRCC 2 cut(s) 1740, 2594
AccBSI CCGCTC 1 cut(s) 1974
AccI GTMKAC 2 cut(s) 1223, 1232
AccII CGCG 1 cut(s) 1006
AciI CCGC 5 cut(s) 779, 1006, 1972, 2348, 2351
AclWI GGATC 7 cut(s) 655, 911, 924, 980, 1094, 2431, 2444
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 1287, 1384
AcuI CTGAAG 3 cut(s) 278, 1410, 2623
AcyI GRCGYC 1 cut(s) 1741
AfaI GTAC 5 cut(s) 263, 1423, 1536, 1555, 2324
AfiI CCNNNNNNNGG 5 cut(s) 901, 2238, 2350, 2351, 2409
AflIII ACRYGT 1 cut(s) 1254
AjnI CCWGG 3 cut(s) 486, 702, 848
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 3 cut(s) 82, 124, 2090
AlwI GGATC 7 cut(s) 655, 911, 924, 980, 1094, 2431, 2444
AlwNI CAGNNNCTG 4 cut(s) 855, 1157, 2375, 2498
Ama87I CYCGRG 3 cut(s) 1458, 1759, 2402
AoxI GGCC 9 cut(s) 3, 484, 682, 982, 1671, 1793, 1944, 2112, 2198
ApeKI GCWGC 9 cut(s) 415, 855, 858, 1492, 2129, 2249, 2492, 2495, 2498
ApoI RAATTY 2 cut(s) 1287, 1384
AseI ATTAAT 1 cut(s) 2031
AspA2I CCTAGG 1 cut(s) 2600
AspLEI GCGC 3 cut(s) 275, 828, 1743
AspS9I GGNCC 8 cut(s) 647, 682, 1268, 1672, 1944, 2198, 2341, 2545
AsuC2I CCSGG 1 cut(s) 251
AsuHPI GGTGA 3 cut(s) 1206, 1689, 2164
AvaI CYCGRG 3 cut(s) 1458, 1759, 2402
AvaII GGWCC 4 cut(s) 647, 1268, 2341, 2545
AvrII CCTAGG 1 cut(s) 2600
BaeI ACNNNNGTAYC 2 cut(s) 1974, 2007
BamHI GGATCC 2 cut(s) 916, 2436
BanI GGYRCC 2 cut(s) 1740, 2594
BauI CACGAG 1 cut(s) 821
BbsI GAAGAC 3 cut(s) 305, 414, 1398
Bbv12I GWGCWC 1 cut(s) 582
BbvCI CCTCAGC 1 cut(s) 2109
BbvI GCAGC 9 cut(s) 427, 842, 870, 1504, 2116, 2261, 2482, 2485, 2504
BccI CCATC 5 cut(s) 160, 1462, 1504, 1756, 2332
BciT130I CCWGG 3 cut(s) 488, 704, 850
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 3 cut(s) 82, 124, 2090
BfaI CTAG 9 cut(s) 426, 665, 1379, 1430, 1530, 1752, 1832, 2327, 2601
BfmI CTRYAG 4 cut(s) 81, 723, 856, 1977
BfoI RGCGCY 3 cut(s) 276, 829, 1744
BfuAI ACCTGC 2 cut(s) 149, 883
BglII AGATCT 1 cut(s) 1426
BlnI CCTAGG 1 cut(s) 2600
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 4 cut(s) 251, 488, 704, 850
Bme18I GGWCC 4 cut(s) 647, 1268, 2341, 2545
BmeT110I CYCGRG 3 cut(s) 1458, 1759, 2402
BmgT120I GGNCC 8 cut(s) 647, 682, 1268, 1672, 1944, 2198, 2341, 2545
BmiI GGNNCC 8 cut(s) 918, 1324, 1674, 1742, 1945, 2343, 2438, 2596
BmrFI CCNGG 4 cut(s) 251, 488, 704, 850
BmrI ACTGGG 1 cut(s) 380
BmsI GCATC 9 cut(s) 145, 301, 532, 557, 607, 941, 1285, 1309, 2236
BmuI ACTGGG 1 cut(s) 380
BpiI GAAGAC 3 cut(s) 305, 414, 1398
BplI GAGNNNNNCTC 2 cut(s) 1276, 1308
BpmI CTGGAG 1 cut(s) 923
Bpu10I CCTNAGC 3 cut(s) 686, 2109, 2630
BpuEI CTTGAG 4 cut(s) 575, 596, 1542, 2114
BpuMI CCSGG 1 cut(s) 251
Bsa29I ATCGAT 1 cut(s) 2027
BsaHI GRCGYC 1 cut(s) 1741
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 5 cut(s) 1668, 1760, 2115, 2403, 2600
BsaWI WCCGGW 1 cut(s) 1102
BsaXI ACNNNNNCTCC 4 cut(s) 1648, 1678, 2610, 2640
Bsc4I CCNNNNNNNGG 5 cut(s) 901, 2238, 2350, 2351, 2409
Bse1I ACTGG 6 cut(s) 375, 416, 920, 974, 1194, 1240
Bse3DI GCAATG 2 cut(s) 315, 566
BseBI CCWGG 3 cut(s) 488, 704, 850
BseCI ATCGAT 1 cut(s) 2027
BseDI CCNNGG 5 cut(s) 1668, 1760, 2115, 2403, 2600
BseGI GGATG 2 cut(s) 622, 1300
BseLI CCNNNNNNNGG 5 cut(s) 901, 2238, 2350, 2351, 2409
BseMI GCAATG 2 cut(s) 315, 566
BseMII CTCAG 7 cut(s) 117, 669, 1209, 1673, 1683, 2100, 2139
BseNI ACTGG 6 cut(s) 375, 416, 920, 974, 1194, 1240
BseRI GAGGAG 1 cut(s) 196
BseXI GCAGC 9 cut(s) 427, 842, 870, 1504, 2116, 2261, 2482, 2485, 2504
BseYI CCCAGC 1 cut(s) 519
BsgI GTGCAG 1 cut(s) 2500
Bsh1236I CGCG 1 cut(s) 1006
BshFI GGCC 9 cut(s) 5, 486, 684, 984, 1673, 1795, 1946, 2114, 2200
BshNI GGYRCC 2 cut(s) 1740, 2594
BshVI ATCGAT 1 cut(s) 2027
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 3 cut(s) 1458, 1759, 2402
BsiSI CCGG 4 cut(s) 251, 1103, 1891, 2592
BslFI GGGAC 2 cut(s) 2214, 2549
BslI CCNNNNNNNGG 5 cut(s) 901, 2238, 2350, 2351, 2409
BsmAI GTCTC 3 cut(s) 82, 124, 2090
BsmFI GGGAC 2 cut(s) 2214, 2549
BsmI GAATGC 1 cut(s) 2005
BsnI GGCC 9 cut(s) 5, 486, 684, 984, 1673, 1795, 1946, 2114, 2200
Bso31I GGTCTC 1 cut(s) 82
BsoBI CYCGRG 3 cut(s) 1458, 1759, 2402
Bsp1286I GDGCHC 1 cut(s) 582
Bsp143I GATC 9 cut(s) 628, 660, 916, 972, 1099, 1426, 1798, 2154, 2436
BspACI CCGC 5 cut(s) 779, 1006, 1972, 2348, 2351
BspANI GGCC 9 cut(s) 5, 486, 684, 984, 1673, 1795, 1946, 2114, 2200
BspCNI CTCAG 7 cut(s) 118, 668, 1210, 1674, 1684, 2101, 2138
BspDI ATCGAT 1 cut(s) 2027
BspFNI CGCG 1 cut(s) 1006
BspHI TCATGA 1 cut(s) 2281
BspLI GGNNCC 8 cut(s) 918, 1324, 1674, 1742, 1945, 2343, 2438, 2596
BspMAI CTGCAG 2 cut(s) 85, 860
BspMI ACCTGC 2 cut(s) 149, 883
BspPI GGATC 7 cut(s) 655, 911, 924, 980, 1094, 2431, 2444
BspQI GCTCTTC 1 cut(s) 45
BspT107I GGYRCC 2 cut(s) 1740, 2594
BspTNI GGTCTC 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 1974
BsrDI GCAATG 2 cut(s) 315, 566
BsrI ACTGG 6 cut(s) 375, 416, 920, 974, 1194, 1240
BssECI CCNNGG 5 cut(s) 1668, 1760, 2115, 2403, 2600
BssMI GATC 9 cut(s) 628, 660, 916, 972, 1099, 1426, 1798, 2154, 2436
BssNI GRCGYC 1 cut(s) 1741
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 3 cut(s) 1668, 2115, 2600
Bst2BI CACGAG 1 cut(s) 821
Bst2UI CCWGG 3 cut(s) 488, 704, 850
Bst4CI ACNGT 7 cut(s) 294, 504, 607, 991, 1048, 1660, 2525
Bst6I CTCTTC 2 cut(s) 45, 273
BstACI GRCGYC 1 cut(s) 1741
BstAPI GCANNNNNTGC 2 cut(s) 1061, 2498
BstC8I GCNNGC 4 cut(s) 846, 1149, 1353, 1439
BstF5I GGATG 2 cut(s) 622, 1300
BstFNI CGCG 1 cut(s) 1006
BstH2I RGCGCY 3 cut(s) 276, 829, 1744
BstHHI GCGC 3 cut(s) 275, 828, 1743
BstKTI GATC 9 cut(s) 631, 663, 919, 975, 1102, 1429, 1801, 2157, 2439
BstMAI GTCTC 3 cut(s) 82, 124, 2090
BstMBI GATC 9 cut(s) 628, 660, 916, 972, 1099, 1426, 1798, 2154, 2436
BstMWI GCNNNNNNNGC 7 cut(s) 1061, 1153, 1670, 2009, 2255, 2381, 2498
BstNI CCWGG 3 cut(s) 488, 704, 850
BstNSI RCATGY 2 cut(s) 1151, 1258
BstSCI CCNGG 4 cut(s) 249, 486, 702, 848
BstSFI CTRYAG 4 cut(s) 81, 723, 856, 1977
BstUI CGCG 1 cut(s) 1006
BstV1I GCAGC 9 cut(s) 427, 842, 870, 1504, 2116, 2261, 2482, 2485, 2504
BstV2I GAAGAC 3 cut(s) 305, 414, 1398
BstX2I RGATCY 4 cut(s) 660, 916, 1426, 2436
BstYI RGATCY 4 cut(s) 660, 916, 1426, 2436
Bsu15I ATCGAT 1 cut(s) 2027
BsuRI GGCC 9 cut(s) 5, 486, 684, 984, 1673, 1795, 1946, 2114, 2200
BsuTUI ATCGAT 1 cut(s) 2027
BtsCI GGATG 2 cut(s) 622, 1300
BtsI GCAGTG 3 cut(s) 1271, 1627, 2058
BtsIMutI CAGTG 7 cut(s) 927, 987, 1044, 1201, 1271, 1627, 2058
BveI ACCTGC 2 cut(s) 149, 883
Cac8I GCNNGC 4 cut(s) 846, 1149, 1353, 1439
CaiI CAGNNNCTG 4 cut(s) 855, 1157, 2375, 2498
CciI TCATGA 1 cut(s) 2281
CfoI GCGC 3 cut(s) 275, 828, 1743
Cfr13I GGNCC 8 cut(s) 647, 682, 1268, 1672, 1944, 2198, 2341, 2545
ClaI ATCGAT 1 cut(s) 2027
CseI GACGC 3 cut(s) 1012, 1509, 1634
Csp6I GTAC 5 cut(s) 262, 1422, 1535, 1554, 2323
CviAII CATG 5 cut(s) 583, 1148, 1255, 2087, 2282
CviQI GTAC 5 cut(s) 262, 1422, 1535, 1554, 2323
DinI GGCGCC 1 cut(s) 1742
DpnI GATC 9 cut(s) 630, 662, 918, 974, 1101, 1428, 1800, 2156, 2438
DpnII GATC 9 cut(s) 628, 660, 916, 972, 1099, 1426, 1798, 2154, 2436
DraI TTTAAA 1 cut(s) 1885
DrdI GACNNNNNNGTC 1 cut(s) 1606
DseDI GACNNNNNNGTC 1 cut(s) 1606
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 45, 273
EarI CTCTTC 2 cut(s) 45, 273
Eco130I CCWWGG 3 cut(s) 1668, 2115, 2600
Eco147I AGGCCT 1 cut(s) 1795
Eco31I GGTCTC 1 cut(s) 82
Eco32I GATATC 4 cut(s) 174, 465, 493, 801
Eco47I GGWCC 4 cut(s) 647, 1268, 2341, 2545
Eco57I CTGAAG 3 cut(s) 278, 1410, 2623
Eco88I CYCGRG 3 cut(s) 1458, 1759, 2402
EcoO109I RGGNCCY 2 cut(s) 682, 2545
EcoRII CCWGG 3 cut(s) 486, 702, 848
EcoRV GATATC 4 cut(s) 174, 465, 493, 801
EcoT14I CCWWGG 3 cut(s) 1668, 2115, 2600
EcoT22I ATGCAT 1 cut(s) 566
EgeI GGCGCC 1 cut(s) 1742
EheI GGCGCC 1 cut(s) 1742
ErhI CCWWGG 3 cut(s) 1668, 2115, 2600
FaeI CATG 5 cut(s) 586, 1151, 1258, 2090, 2285
FalI AAGNNNNNCTT 4 cut(s) 177, 209, 423, 455
FaqI GGGAC 2 cut(s) 2214, 2549
FatI CATG 5 cut(s) 582, 1147, 1254, 2086, 2281
FauI CCCGC 1 cut(s) 2344
FblI GTMKAC 2 cut(s) 1223, 1232
FokI GGATG 2 cut(s) 629, 1287
FspBI CTAG 9 cut(s) 426, 665, 1379, 1430, 1530, 1752, 1832, 2327, 2601
GlaI GCGC 3 cut(s) 274, 827, 1742
GsaI CCCAGC 1 cut(s) 523
GsuI CTGGAG 1 cut(s) 923
HaeII RGCGCY 3 cut(s) 276, 829, 1744
HaeIII GGCC 9 cut(s) 5, 486, 684, 984, 1673, 1795, 1946, 2114, 2200
HapII CCGG 4 cut(s) 251, 1103, 1891, 2592
HgaI GACGC 3 cut(s) 1012, 1509, 1634
HhaI GCGC 3 cut(s) 275, 828, 1743
Hin1I GRCGYC 1 cut(s) 1741
Hin1II CATG 5 cut(s) 586, 1151, 1258, 2090, 2285
Hin6I GCGC 3 cut(s) 273, 826, 1741
HinP1I GCGC 3 cut(s) 273, 826, 1741
HincII GTYRAC 2 cut(s) 603, 2362
HindII GTYRAC 2 cut(s) 603, 2362
HpaII CCGG 4 cut(s) 251, 1103, 1891, 2592
HphI GGTGA 3 cut(s) 1206, 1689, 2164
Hpy166II GTNNAC 5 cut(s) 603, 1224, 1233, 1271, 2362
Hpy8I GTNNAC 5 cut(s) 603, 1224, 1233, 1271, 2362
Hpy99I CGWCG 1 cut(s) 1603
HpyAV CCTTC 7 cut(s) 736, 1148, 1313, 2175, 2403, 2476, 2549
HpyCH4III ACNGT 7 cut(s) 294, 504, 607, 991, 1048, 1660, 2525
HpyF10VI GCNNNNNNNGC 7 cut(s) 1061, 1153, 1670, 2009, 2255, 2381, 2498
Hsp92I GRCGYC 1 cut(s) 1741
Hsp92II CATG 5 cut(s) 586, 1151, 1258, 2090, 2285
HspAI GCGC 3 cut(s) 273, 826, 1741
KasI GGCGCC 1 cut(s) 1740
Kzo9I GATC 9 cut(s) 628, 660, 916, 972, 1099, 1426, 1798, 2154, 2436
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 6 cut(s) 397, 852, 904, 1669, 1974, 2558
Lsp1109I GCAGC 9 cut(s) 427, 842, 870, 1504, 2116, 2261, 2482, 2485, 2504
LweI GCATC 9 cut(s) 145, 301, 532, 557, 607, 941, 1285, 1309, 2236
MaeI CTAG 9 cut(s) 426, 665, 1379, 1430, 1530, 1752, 1832, 2327, 2601
MaeIII GTNAC 8 cut(s) 808, 1212, 1622, 1695, 1826, 2005, 2525, 2583
MalI GATC 9 cut(s) 630, 662, 918, 974, 1101, 1428, 1800, 2156, 2438
MbiI CCGCTC 1 cut(s) 1974
MboI GATC 9 cut(s) 628, 660, 916, 972, 1099, 1426, 1798, 2154, 2436
MfeI CAATTG 3 cut(s) 2052, 2070, 2418
MflI RGATCY 4 cut(s) 660, 916, 1426, 2436
MhlI GDGCHC 1 cut(s) 582
Mly113I GGCGCC 1 cut(s) 1741
MlyI GAGTC 6 cut(s) 295, 1085, 1229, 1703, 1724, 2236
MmeI TCCRAC 2 cut(s) 625, 1321
Mph1103I ATGCAT 1 cut(s) 566
MseI TTAA 9 cut(s) 195, 287, 612, 1121, 1542, 1884, 2019, 2031, 2265
MslI CAYNNNNRTG 1 cut(s) 1194
MspA1I CMGCKG 2 cut(s) 2129, 2495
MspI CCGG 4 cut(s) 251, 1103, 1891, 2592
MspR9I CCNGG 4 cut(s) 251, 488, 704, 850
MunI CAATTG 3 cut(s) 2052, 2070, 2418
Mva1269I GAATGC 1 cut(s) 2005
MvaI CCWGG 3 cut(s) 488, 704, 850
MvnI CGCG 1 cut(s) 1006
MwoI GCNNNNNNNGC 7 cut(s) 1061, 1153, 1670, 2009, 2255, 2381, 2498
NarI GGCGCC 1 cut(s) 1741
NciI CCSGG 1 cut(s) 251
NdeII GATC 9 cut(s) 628, 660, 916, 972, 1099, 1426, 1798, 2154, 2436
NlaIII CATG 5 cut(s) 586, 1151, 1258, 2090, 2285
NlaIV GGNNCC 8 cut(s) 918, 1324, 1674, 1742, 1945, 2343, 2438, 2596
NmuCI GTSAC 5 cut(s) 1212, 1622, 1695, 2005, 2583
NsiI ATGCAT 1 cut(s) 566
NspI RCATGY 2 cut(s) 1151, 1258
PaeI GCATGC 1 cut(s) 1151
PaeR7I CTCGAG 1 cut(s) 1458
PagI TCATGA 1 cut(s) 2281
PaqCI CACCTGC 1 cut(s) 149
PceI AGGCCT 1 cut(s) 1795
PciI ACATGT 1 cut(s) 1254
PciSI GCTCTTC 1 cut(s) 45
PctI GAATGC 1 cut(s) 2005
PfeI GAWTC 7 cut(s) 323, 455, 709, 1172, 1205, 1603, 2083
PleI GAGTC 6 cut(s) 295, 1084, 1228, 1702, 1723, 2235
PluTI GGCGCC 1 cut(s) 1744
PpsI GAGTC 6 cut(s) 295, 1084, 1228, 1702, 1723, 2235
PpuMI RGGWCCY 1 cut(s) 2545
PscI ACATGT 1 cut(s) 1254
PshBI ATTAAT 1 cut(s) 2031
Psp5II RGGWCCY 1 cut(s) 2545
Psp6I CCWGG 3 cut(s) 486, 702, 848
PspFI CCCAGC 1 cut(s) 519
PspGI CCWGG 3 cut(s) 486, 702, 848
PspN4I GGNNCC 8 cut(s) 918, 1324, 1674, 1742, 1945, 2343, 2438, 2596
PspPI GGNCC 8 cut(s) 647, 682, 1268, 1672, 1944, 2198, 2341, 2545
PspPPI RGGWCCY 1 cut(s) 2545
PstI CTGCAG 2 cut(s) 85, 860
PstNI CAGNNNCTG 4 cut(s) 855, 1157, 2375, 2498
PsuI RGATCY 4 cut(s) 660, 916, 1426, 2436
PvuII CAGCTG 2 cut(s) 2129, 2495
RsaI GTAC 5 cut(s) 263, 1423, 1536, 1555, 2324
RsaNI GTAC 5 cut(s) 262, 1422, 1535, 1554, 2323
RseI CAYNNNNRTG 1 cut(s) 1194
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 9 cut(s) 195, 287, 612, 1121, 1542, 1884, 2019, 2031, 2265
Sau3AI GATC 9 cut(s) 628, 660, 916, 972, 1099, 1426, 1798, 2154, 2436
Sau96I GGNCC 8 cut(s) 647, 682, 1268, 1672, 1944, 2198, 2341, 2545
ScaI AGTACT 1 cut(s) 263
SchI GAGTC 6 cut(s) 295, 1085, 1229, 1703, 1724, 2236
ScrFI CCNGG 4 cut(s) 251, 488, 704, 850
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 9 cut(s) 145, 301, 532, 557, 607, 941, 1285, 1309, 2236
SfcI CTRYAG 4 cut(s) 81, 723, 856, 1977
SfoI GGCGCC 1 cut(s) 1742
Sfr274I CTCGAG 1 cut(s) 1458
SinI GGWCC 4 cut(s) 647, 1268, 2341, 2545
SlaI CTCGAG 1 cut(s) 1458
SmiMI CAYNNNNRTG 1 cut(s) 1194
SmlI CTYRAG 5 cut(s) 554, 575, 1458, 1557, 2093
SmoI CTYRAG 5 cut(s) 554, 575, 1458, 1557, 2093
SphI GCATGC 1 cut(s) 1151
SseBI AGGCCT 1 cut(s) 1795
SsiI CCGC 5 cut(s) 779, 1006, 1972, 2348, 2351
SspDI GGCGCC 1 cut(s) 1740
SspMI CTAG 9 cut(s) 426, 665, 1379, 1430, 1530, 1752, 1832, 2327, 2601
StuI AGGCCT 1 cut(s) 1795
StyD4I CCNGG 4 cut(s) 249, 486, 702, 848
StyI CCWWGG 3 cut(s) 1668, 2115, 2600
TaaI ACNGT 7 cut(s) 294, 504, 607, 991, 1048, 1660, 2525
TaqI TCGA 7 cut(s) 1459, 1598, 1726, 2027, 2157, 2393, 2451
TaqII GACCGA 2 cut(s) 965, 1218
TatI WGTACW 2 cut(s) 261, 1421
TauI GCSGC 2 cut(s) 1009, 2351
TfiI GAWTC 7 cut(s) 323, 455, 709, 1172, 1205, 1603, 2083
Tru1I TTAA 9 cut(s) 195, 287, 612, 1121, 1542, 1884, 2019, 2031, 2265
Tru9I TTAA 9 cut(s) 195, 287, 612, 1121, 1542, 1884, 2019, 2031, 2265
TscAI CASTG 7 cut(s) 927, 994, 1051, 1201, 1278, 1627, 2065
TseFI GTSAC 5 cut(s) 1212, 1622, 1695, 2005, 2583
TseI GCWGC 9 cut(s) 415, 855, 858, 1492, 2129, 2249, 2492, 2495, 2498
Tsp45I GTSAC 5 cut(s) 1212, 1622, 1695, 2005, 2583
TspDTI ATGAA 9 cut(s) 357, 549, 606, 949, 1218, 1251, 1479, 2075, 2270
TspGWI ACGGA 1 cut(s) 929
TspRI CASTG 7 cut(s) 927, 994, 1051, 1201, 1278, 1627, 2065
VpaK11BI GGWCC 4 cut(s) 647, 1268, 2341, 2545
VspI ATTAAT 1 cut(s) 2031
XapI RAATTY 2 cut(s) 1287, 1384
XbaI TCTAGA 1 cut(s) 1429
XceI RCATGY 2 cut(s) 1151, 1258
XcmI CCANNNNNNNNNTGG 2 cut(s) 1909, 1954
XhoI CTCGAG 1 cut(s) 1458
XmaJI CCTAGG 1 cut(s) 2600
XmiI GTMKAC 2 cut(s) 1223, 1232
XspI CTAG 9 cut(s) 426, 665, 1379, 1430, 1530, 1752, 1832, 2327, 2601
ZrmI AGTACT 1 cut(s) 263
Zsp2I ATGCAT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.