pycom15g33240

5'-adenylylsulfate reductase-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008540
Physical Location & Seq
Reverse (-)
32528105 .. 32530254
2150 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 942 bp
ATGGGGATTAGGGTTTGGGAAAGTGGGATCGCGATATTGGTTTTATGGGTCCGGCTAATTTCCGCCGCTGAACCCACCTCTTGCCCCGCGGAGTCCGCAGCCGATGCGATCTTCGGCTTCCGGTATTCCAATTGCCCCGTCAACGGCGCTTCCAGATCCGTTGAATCCATGGGTGTTATCGTGGGGGATGAGGTTTCTTTACAAAGGGCATTAAATATGGTTCACAAGAATACTCATGATTATGTGGCGGTGCTCTTCTATGCATCATGGTGCCCTTTCTCAAGGATGTTTAAACCAACATTTTCCATCCTGGCTTCTTTGTATCCTTCGATCCCACATTTTGCATTTGAAGAATCAGCCATCAGGCCAAGTATACTCTCTAAATATGGAGTTCATGGATTTCCTACTCTTTTCATTTTGAATTCTACCACGCGCATTCGATATCAAGGCGCCCGCACTCCTGGTTCTCTTATTGCTTTCTATAGTCATGTTACTGGCATCAAGACTGTATCACTGGATCAATTGTCACTGGAAAAAATTGGATACCCACTAAATCATGAGAAGCATGATAGCACTGAGCAGGAAAGCTGCCCGTTTTCGTGGGATCGATCTCCAGAGAATTTGCTTCGGCAAGAAACATATTTAGCTCTGGCTTCTGCTTTTGTGCTTCTGAGATTGCTCTACTCCTTTTATCCTGCTCTGCTTTCGTTTGTTCAATCTGCATGGAGACAGCATATTCAAAACATGAGACTGGGAAGCTTGTTGGAGCACCCTTTGGCTTTTCTAAAGCGGGCAGTACAGTTGTTTAATTCTCTAAAAGAGCCTTGCAAGAGGAGTAATTTGCAGGAAGGAGCAATGAACGCCAGGGTGTGGGCTTCCAAGTCACTGGCCACAGTTTCTATCGGGGATGCAAGCACCAGCAGGGGTTATAGCAGTGACTGA

Protein Analysis

314

Amino Acids

34.93

Weight (kDa)

8.93

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 77 - 160 1.1e-09 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 270, 449
AccB7I CCANNNNNTGG 1 cut(s) 870
AccI GTMKAC 1 cut(s) 373
AccII CGCG 3 cut(s) 32, 89, 433
AciI CCGC 8 cut(s) 63, 66, 87, 89, 96, 248, 454, 790
AclWI GGATC 5 cut(s) 35, 150, 325, 525, 612
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 2 cut(s) 421, 619
AcyI GRCGYC 1 cut(s) 450
AfaI GTAC 1 cut(s) 798
AfiI CCNNNNNNNGG 2 cut(s) 143, 870
AgsI TTSAA 5 cut(s) 164, 350, 421, 716, 740
AjnI CCWGG 3 cut(s) 309, 460, 863
AluBI AGCT 3 cut(s) 588, 647, 759
AluI AGCT 3 cut(s) 588, 647, 759
Alw21I GWGCWC 2 cut(s) 255, 771
Alw26I GTCTC 2 cut(s) 721, 742
AlwI GGATC 5 cut(s) 35, 150, 325, 525, 612
AlwNI CAGNNNCTG 1 cut(s) 939
AoxI GGCC 2 cut(s) 365, 888
ApeKI GCWGC 2 cut(s) 98, 588
ApoI RAATTY 2 cut(s) 421, 619
AspLEI GCGC 3 cut(s) 149, 435, 452
AspS9I GGNCC 1 cut(s) 49
AvaII GGWCC 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 275
BalI TGGCCA 1 cut(s) 890
BanI GGYRCC 2 cut(s) 270, 449
Bbv12I GWGCWC 2 cut(s) 255, 771
BbvI GCAGC 2 cut(s) 110, 575
BccI CCATC 2 cut(s) 314, 368
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 3 cut(s) 311, 462, 865
BciVI GTATCC 2 cut(s) 333, 536
BcoDI GTCTC 2 cut(s) 721, 742
BfmI CTRYAG 1 cut(s) 481
BfoI RGCGCY 2 cut(s) 150, 453
BfuI GTATCC 2 cut(s) 333, 536
BisI GCNGC 3 cut(s) 66, 99, 589
BlsI GCNGC 3 cut(s) 67, 100, 590
Bme1390I CCNGG 3 cut(s) 311, 462, 865
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 3 cut(s) 50, 272, 451
BmrFI CCNGG 3 cut(s) 311, 462, 865
BmrI ACTGGG 1 cut(s) 761
BmsI GCATC 4 cut(s) 94, 272, 507, 898
BmuI ACTGGG 1 cut(s) 761
BpmI CTGGAG 1 cut(s) 597
BpuEI CTTGAG 1 cut(s) 265
Bsa29I ATCGAT 1 cut(s) 607
BsaHI GRCGYC 1 cut(s) 450
BsaJI CCNNGG 3 cut(s) 87, 168, 864
BsaWI WCCGGW 1 cut(s) 120
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 870
Bse1I ACTGG 5 cut(s) 499, 519, 534, 756, 891
Bse3DI GCAATG 1 cut(s) 861
BseBI CCWGG 3 cut(s) 311, 462, 865
BseCI ATCGAT 1 cut(s) 607
BseDI CCNNGG 3 cut(s) 87, 168, 864
BseGI GGATG 4 cut(s) 193, 291, 306, 913
BseLI CCNNNNNNNGG 2 cut(s) 143, 870
BseMI GCAATG 1 cut(s) 861
BseMII CTCAG 2 cut(s) 567, 662
BseNI ACTGG 5 cut(s) 499, 519, 534, 756, 891
BseRI GAGGAG 1 cut(s) 847
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 2 cut(s) 110, 575
Bsh1236I CGCG 3 cut(s) 32, 89, 433
BshFI GGCC 2 cut(s) 367, 890
BshNI GGYRCC 2 cut(s) 270, 449
BshVI ATCGAT 1 cut(s) 607
BsiHKAI GWGCWC 2 cut(s) 255, 771
BsiSI CCGG 2 cut(s) 52, 121
BslI CCNNNNNNNGG 2 cut(s) 143, 870
BsmAI GTCTC 2 cut(s) 721, 742
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 2 cut(s) 367, 890
Bsp1286I GDGCHC 3 cut(s) 255, 275, 771
Bsp143I GATC 7 cut(s) 27, 108, 155, 330, 517, 604, 608
Bsp19I CCATGG 1 cut(s) 168
Bsp68I TCGCGA 1 cut(s) 32
BspACI CCGC 8 cut(s) 63, 66, 87, 89, 96, 248, 454, 790
BspANI GGCC 2 cut(s) 367, 890
BspCNI CTCAG 2 cut(s) 568, 663
BspDI ATCGAT 1 cut(s) 607
BspFNI CGCG 3 cut(s) 32, 89, 433
BspHI TCATGA 2 cut(s) 235, 556
BspLI GGNNCC 3 cut(s) 50, 272, 451
BspPI GGATC 5 cut(s) 35, 150, 325, 525, 612
BspQI GCTCTTC 1 cut(s) 260
BspT107I GGYRCC 2 cut(s) 270, 449
BsrDI GCAATG 1 cut(s) 861
BsrI ACTGG 5 cut(s) 499, 519, 534, 756, 891
BssECI CCNNGG 3 cut(s) 87, 168, 864
BssMI GATC 7 cut(s) 27, 108, 155, 330, 517, 604, 608
BssNAI GTATAC 1 cut(s) 374
BssNI GRCGYC 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 168
Bst1107I GTATAC 1 cut(s) 374
Bst2UI CCWGG 3 cut(s) 311, 462, 865
Bst4CI ACNGT 3 cut(s) 508, 801, 895
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 1 cut(s) 450
BstAPI GCANNNNNTGC 1 cut(s) 104
BstC8I GCNNGC 3 cut(s) 454, 792, 913
BstDEI CTNAG 2 cut(s) 576, 671
BstDSI CCRYGG 2 cut(s) 87, 168
BstF5I GGATG 4 cut(s) 193, 291, 306, 913
BstFNI CGCG 3 cut(s) 32, 89, 433
BstH2I RGCGCY 2 cut(s) 150, 453
BstHHI GCGC 3 cut(s) 149, 435, 452
BstKTI GATC 7 cut(s) 30, 111, 158, 333, 520, 607, 611
BstMAI GTCTC 2 cut(s) 721, 742
BstMBI GATC 7 cut(s) 27, 108, 155, 330, 517, 604, 608
BstMWI GCNNNNNNNGC 3 cut(s) 95, 104, 860
BstNI CCWGG 3 cut(s) 311, 462, 865
BstSCI CCNGG 3 cut(s) 309, 460, 863
BstSFI CTRYAG 1 cut(s) 481
BstSLI GKGCMC 1 cut(s) 275
BstUI CGCG 3 cut(s) 32, 89, 433
BstV1I GCAGC 2 cut(s) 110, 575
BstX2I RGATCY 1 cut(s) 155
BstXI CCANNNNNNTGG 1 cut(s) 886
BstYI RGATCY 1 cut(s) 155
BstZ17I GTATAC 1 cut(s) 374
Bsu15I ATCGAT 1 cut(s) 607
BsuI GTATCC 2 cut(s) 333, 536
BsuRI GGCC 2 cut(s) 367, 890
BsuTUI ATCGAT 1 cut(s) 607
BtgI CCRYGG 2 cut(s) 87, 168
BtsCI GGATG 4 cut(s) 193, 291, 306, 913
BtsI GCAGTG 1 cut(s) 940
BtsIMutI CAGTG 5 cut(s) 512, 527, 573, 884, 940
BtuMI TCGCGA 1 cut(s) 32
Cac8I GCNNGC 3 cut(s) 454, 792, 913
CaiI CAGNNNCTG 1 cut(s) 939
CciI TCATGA 2 cut(s) 235, 556
CfoI GCGC 3 cut(s) 149, 435, 452
Cfr13I GGNCC 1 cut(s) 49
Cfr42I CCGCGG 1 cut(s) 90
ClaI ATCGAT 1 cut(s) 607
Csp6I GTAC 1 cut(s) 797
CviAII CATG 9 cut(s) 169, 236, 267, 395, 488, 557, 566, 723, 745
CviQI GTAC 1 cut(s) 797
DdeI CTNAG 2 cut(s) 576, 671
DinI GGCGCC 1 cut(s) 451
DpnI GATC 7 cut(s) 29, 110, 157, 332, 519, 606, 610
DpnII GATC 7 cut(s) 27, 108, 155, 330, 517, 604, 608
DraI TTTAAA 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
EciI GGCGGA 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 168
Eco32I GATATC 1 cut(s) 443
Eco47I GGWCC 1 cut(s) 49
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 3 cut(s) 309, 460, 863
EcoRV GATATC 1 cut(s) 443
EcoT14I CCWWGG 1 cut(s) 168
EcoT22I ATGCAT 1 cut(s) 265
EgeI GGCGCC 1 cut(s) 451
EheI GGCGCC 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 9 cut(s) 172, 239, 270, 398, 491, 560, 569, 726, 748
FatI CATG 9 cut(s) 168, 235, 266, 394, 487, 556, 565, 722, 744
FauI CCCGC 3 cut(s) 94, 461, 783
FblI GTMKAC 1 cut(s) 373
Fnu4HI GCNGC 3 cut(s) 66, 99, 589
FokI GGATG 4 cut(s) 200, 293, 298, 920
Fsp4HI GCNGC 3 cut(s) 66, 99, 589
GlaI GCGC 3 cut(s) 148, 434, 451
GluI GCNGC 3 cut(s) 66, 99, 589
GsuI CTGGAG 1 cut(s) 597
HaeII RGCGCY 2 cut(s) 150, 453
HaeIII GGCC 2 cut(s) 367, 890
HapII CCGG 2 cut(s) 52, 121
HhaI GCGC 3 cut(s) 149, 435, 452
Hin1I GRCGYC 1 cut(s) 450
Hin1II CATG 9 cut(s) 172, 239, 270, 398, 491, 560, 569, 726, 748
Hin6I GCGC 3 cut(s) 147, 433, 450
HinP1I GCGC 3 cut(s) 147, 433, 450
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HindIII AAGCTT 1 cut(s) 757
HinfI GANTC 3 cut(s) 92, 164, 353
HpaII CCGG 2 cut(s) 52, 121
Hpy166II GTNNAC 3 cut(s) 142, 223, 374
Hpy188I TCNGA 1 cut(s) 672
Hpy188III TCNNGA 6 cut(s) 31, 153, 236, 502, 557, 614
Hpy8I GTNNAC 3 cut(s) 142, 223, 374
HpyAV CCTTC 2 cut(s) 336, 842
HpyCH4III ACNGT 3 cut(s) 508, 801, 895
HpyCH4V TGCA 6 cut(s) 263, 344, 722, 828, 844, 911
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 104, 860
HpyF3I CTNAG 2 cut(s) 576, 671
Hsp92I GRCGYC 1 cut(s) 450
Hsp92II CATG 9 cut(s) 172, 239, 270, 398, 491, 560, 569, 726, 748
HspAI GCGC 3 cut(s) 147, 433, 450
KasI GGCGCC 1 cut(s) 449
KspI CCGCGG 1 cut(s) 90
Kzo9I GATC 7 cut(s) 27, 108, 155, 330, 517, 604, 608
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 2 cut(s) 766, 851
Lsp1109I GCAGC 2 cut(s) 110, 575
LweI GCATC 4 cut(s) 94, 272, 507, 898
MaeIII GTNAC 4 cut(s) 490, 525, 882, 935
MalI GATC 7 cut(s) 29, 110, 157, 332, 519, 606, 610
MboI GATC 7 cut(s) 27, 108, 155, 330, 517, 604, 608
MboII GAAGA 3 cut(s) 103, 247, 362
MfeI CAATTG 2 cut(s) 130, 521
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 3 cut(s) 255, 275, 771
MlsI TGGCCA 1 cut(s) 890
MluCI AATT 8 cut(s) 57, 130, 421, 521, 537, 619, 808, 838
MluNI TGGCCA 1 cut(s) 890
Mly113I GGCGCC 1 cut(s) 450
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 744
MnlI CCTC 3 cut(s) 88, 184, 825
Mox20I TGGCCA 1 cut(s) 890
Mph1103I ATGCAT 1 cut(s) 265
MscI TGGCCA 1 cut(s) 890
MseI TTAA 3 cut(s) 212, 291, 807
MslI CAYNNNNRTG 2 cut(s) 240, 268
Msp20I TGGCCA 1 cut(s) 890
MspA1I CMGCKG 2 cut(s) 68, 89
MspI CCGG 2 cut(s) 52, 121
MspR9I CCNGG 3 cut(s) 311, 462, 865
MssI GTTTAAAC 1 cut(s) 292
MunI CAATTG 2 cut(s) 130, 521
Mva1269I GAATGC 1 cut(s) 435
MvaI CCWGG 3 cut(s) 311, 462, 865
MvnI CGCG 3 cut(s) 32, 89, 433
MwoI GCNNNNNNNGC 3 cut(s) 95, 104, 860
NarI GGCGCC 1 cut(s) 450
NcoI CCATGG 1 cut(s) 168
NdeII GATC 7 cut(s) 27, 108, 155, 330, 517, 604, 608
NlaIII CATG 9 cut(s) 172, 239, 270, 398, 491, 560, 569, 726, 748
NlaIV GGNNCC 3 cut(s) 50, 272, 451
NmuCI GTSAC 3 cut(s) 525, 882, 935
NruI TCGCGA 1 cut(s) 32
NsiI ATGCAT 1 cut(s) 265
PagI TCATGA 2 cut(s) 235, 556
PciSI GCTCTTC 1 cut(s) 260
PctI GAATGC 1 cut(s) 435
PfeI GAWTC 2 cut(s) 164, 353
PflMI CCANNNNNTGG 1 cut(s) 870
PkrI GCNGC 3 cut(s) 67, 100, 590
PleI GAGTC 1 cut(s) 100
PluTI GGCGCC 1 cut(s) 453
PmeI GTTTAAAC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 100
Psp6I CCWGG 3 cut(s) 309, 460, 863
PspGI CCWGG 3 cut(s) 309, 460, 863
PspN4I GGNNCC 3 cut(s) 50, 272, 451
PspPI GGNCC 1 cut(s) 49
PstNI CAGNNNCTG 1 cut(s) 939
PsuI RGATCY 1 cut(s) 155
RruI TCGCGA 1 cut(s) 32
RsaI GTAC 1 cut(s) 798
RsaNI GTAC 1 cut(s) 797
RseI CAYNNNNRTG 2 cut(s) 240, 268
SacII CCGCGG 1 cut(s) 90
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 3 cut(s) 212, 291, 807
SatI GCNGC 3 cut(s) 66, 99, 589
Sau3AI GATC 7 cut(s) 27, 108, 155, 330, 517, 604, 608
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 3 cut(s) 311, 462, 865
SduI GDGCHC 3 cut(s) 255, 275, 771
SetI ASST 5 cut(s) 80, 195, 590, 649, 761
SfaNI GCATC 4 cut(s) 94, 272, 507, 898
SfcI CTRYAG 1 cut(s) 481
SfoI GGCGCC 1 cut(s) 451
Sfr303I CCGCGG 1 cut(s) 90
SgrBI CCGCGG 1 cut(s) 90
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 2 cut(s) 240, 268
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 8 cut(s) 57, 130, 421, 521, 537, 619, 808, 838
SsiI CCGC 8 cut(s) 63, 66, 87, 89, 96, 248, 454, 790
SspDI GGCGCC 1 cut(s) 449
StyD4I CCNGG 3 cut(s) 309, 460, 863
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 3 cut(s) 508, 801, 895
TaqI TCGA 3 cut(s) 329, 439, 607
TasI AATT 8 cut(s) 57, 130, 421, 521, 537, 619, 808, 838
TatI WGTACW 1 cut(s) 796
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 2 cut(s) 164, 353
Tru1I TTAA 3 cut(s) 212, 291, 807
Tru9I TTAA 3 cut(s) 212, 291, 807
TscAI CASTG 5 cut(s) 519, 534, 580, 891, 940
TseFI GTSAC 3 cut(s) 525, 882, 935
TseI GCWGC 2 cut(s) 98, 588
Tsp45I GTSAC 3 cut(s) 525, 882, 935
TspDTI ATGAA 3 cut(s) 383, 403, 872
TspGWI ACGGA 1 cut(s) 148
TspRI CASTG 5 cut(s) 519, 534, 580, 891, 940
Van91I CCANNNNNTGG 1 cut(s) 870
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 2 cut(s) 421, 619
XmiI GTMKAC 1 cut(s) 373
Zsp2I ATGCAT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.