pycom15g34080

Dihydrofolate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008449
Physical Location & Seq
Forward (+)
33677028 .. 33678835
1808 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 933 bp
ATGACGTCATCTGGCACATCAAACACCGTGGTCGTTTACATTGAATTTGACCGAGCGAGATTTCCACCCGACGTCGTCAATGTCAAAACTGCCCTCCAACCTGCTCCTTTTAAATCCAGCCAAATTACACACGCATTCCATCCCTCCTCCCACACTACCGGAGCTGCCGAGAAGAACAACAAAAGCCTCGTCGACCGGTGGGAATATTCTTCCTCTCCTTATTATCTCCGAAAAACAGCAGAAAACATGGGGAGCCAAGGAAGCGGAGAAGGCGGCGGGTGTTCGAGGAAGCCCAGGTTTCTGTGCCTCCATGGGTTCCGAACGAGCGGGGAAATAATGAGGAAGCAAGTGGGCAAGTGGCCGGAATCGGTGCTCGAGAAGCTCGACATGGTTTATCTCGATGGGCCCTTTCCGGCCCAAGGTAAATCCGACGTCGACGGCATTTTTGATCCTCCTTATTACGAGTGGTTCCAATTCAACCAGGAATTTTCAGCGTACACGAACTTTGATAAGTGTCTGGAATACATAGAAGATTACATGATTAAACAGGGACCGTTCGACGGCCTTGTTGGTTTTTCCCAGGGAGCAATACTGTCGGCGGGTCTGCCGGGGCTGCAAGCGAAGGGTGTGGCATTGACTAAAGTTCCCAAAATTAAATTCCTGATAATAATCGGAGGGGCGAAATTCAAGTCACCGGCGGTGGCTGAGGATGCTTATTCGTCTCCCATTCAGTGCCCGTCCCTTCACTTTTTAGGGGAGATGGATTTCTTGAAGCCGTACGGGCTTCAACTGTTGGAACACTGTGTAGATGCTTCGGTGATTCATCATCCAAAAGGCCACACTGTTCCAAGACTTGATGAAAAGGGTTTGGATACGATGATGAACTTCATCGACAAGATTCAGAACATATTGGCGGACAAAGACCAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FSH1

Protein Analysis

311

Amino Acids

34.42

Weight (kDa)

6.31

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FSH1 PF03959 95 - 287 6.1e-54 Serine hydrolase (FSH1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 8, 75, 435
Acc36I ACCTGC 1 cut(s) 109
AccBSI CCGCTC 1 cut(s) 327
AccI GTMKAC 2 cut(s) 192, 435
AciI CCGC 7 cut(s) 264, 273, 276, 327, 599, 698, 914
AclWI GGATC 1 cut(s) 443
AcoI YGGCCR 1 cut(s) 359
AcsI RAATTY 4 cut(s) 44, 485, 656, 683
AcyI GRCGYC 3 cut(s) 5, 72, 432
AfaI GTAC 2 cut(s) 497, 779
AfiI CCNNNNNNNGG 2 cut(s) 419, 560
AgeI ACCGGT 1 cut(s) 195
AgsI TTSAA 5 cut(s) 44, 478, 688, 772, 788
AjnI CCWGG 3 cut(s) 293, 480, 579
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AluBI AGCT 2 cut(s) 164, 382
AluI AGCT 2 cut(s) 164, 382
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 1 cut(s) 726
AlwI GGATC 1 cut(s) 443
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 5 cut(s) 359, 404, 414, 562, 835
ApaI GGGCCC 1 cut(s) 408
ApeKI GCWGC 2 cut(s) 164, 613
ApoI RAATTY 4 cut(s) 44, 485, 656, 683
ArsI GACNNNNNNTTYG 6 cut(s) 174, 206, 428, 460, 674, 706
AsiGI ACCGGT 1 cut(s) 195
AspS9I GGNCC 4 cut(s) 404, 405, 415, 551
AsuC2I CCSGG 1 cut(s) 609
AsuHPI GGTGA 2 cut(s) 684, 829
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 1 cut(s) 551
BaeGI GKGCMC 2 cut(s) 408, 737
BanII GRGCYC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 375
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 2 cut(s) 151, 600
BccI CCATC 3 cut(s) 147, 395, 754
BceAI ACGGC 3 cut(s) 454, 577, 760
BcgI CGANNNNNNTGC 2 cut(s) 576, 610
BciT130I CCWGG 3 cut(s) 295, 482, 581
BciVI GTATCC 1 cut(s) 865
BcnI CCSGG 1 cut(s) 609
BcoDI GTCTC 1 cut(s) 726
BfuAI ACCTGC 1 cut(s) 109
BfuI GTATCC 1 cut(s) 865
BglI GCCNNNNNGGC 1 cut(s) 781
BisI GCNGC 3 cut(s) 165, 274, 614
BlsI GCNGC 3 cut(s) 166, 275, 615
Bme1390I CCNGG 4 cut(s) 295, 482, 581, 609
Bme18I GGWCC 1 cut(s) 551
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 4 cut(s) 404, 405, 415, 551
BmiI GGNNCC 5 cut(s) 254, 317, 406, 470, 552
BmrFI CCNGG 4 cut(s) 295, 482, 581, 609
BmsI GCATC 2 cut(s) 700, 799
Bpu10I CCTNAGC 1 cut(s) 705
BpuMI CCSGG 1 cut(s) 609
BsaBI GATNNNNATC 1 cut(s) 668
BsaHI GRCGYC 3 cut(s) 5, 72, 432
BsaJI CCNNGG 8 cut(s) 27, 256, 293, 310, 418, 579, 580, 608
BsaWI WCCGGW 2 cut(s) 158, 195
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 560
Bse118I RCCGGY 2 cut(s) 195, 694
Bse8I GATNNNNATC 1 cut(s) 668
BseBI CCWGG 3 cut(s) 295, 482, 581
BseDI CCNNGG 8 cut(s) 27, 256, 293, 310, 418, 579, 580, 608
BseGI GGATG 3 cut(s) 139, 715, 826
BseJI GATNNNNATC 1 cut(s) 668
BseLI CCNNNNNNNGG 2 cut(s) 419, 560
BseMII CTCAG 1 cut(s) 696
BseRI GAGGAG 1 cut(s) 136
BseSI GKGCMC 2 cut(s) 408, 737
BseXI GCAGC 2 cut(s) 151, 600
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 5 cut(s) 361, 406, 416, 564, 837
BshTI ACCGGT 1 cut(s) 195
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 6 cut(s) 159, 196, 362, 413, 608, 695
BsiWI CGTACG 1 cut(s) 777
BslFI GGGAC 2 cut(s) 564, 724
BslI CCNNNNNNNGG 2 cut(s) 419, 560
BsmAI GTCTC 1 cut(s) 726
BsmBI CGTCTC 1 cut(s) 726
BsmFI GGGAC 2 cut(s) 564, 724
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 5 cut(s) 361, 406, 416, 564, 837
BsoBI CYCGRG 1 cut(s) 374
Bsp120I GGGCCC 1 cut(s) 404
Bsp1286I GDGCHC 3 cut(s) 375, 408, 737
Bsp143I GATC 1 cut(s) 448
Bsp19I CCATGG 1 cut(s) 310
BspACI CCGC 7 cut(s) 264, 273, 276, 327, 599, 698, 914
BspANI GGCC 5 cut(s) 361, 406, 416, 564, 837
BspCNI CTCAG 1 cut(s) 697
BspLI GGNNCC 5 cut(s) 254, 317, 406, 470, 552
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 327
BsrFI RCCGGY 2 cut(s) 195, 694
BssAI RCCGGY 2 cut(s) 195, 694
BssECI CCNNGG 8 cut(s) 27, 256, 293, 310, 418, 579, 580, 608
BssMI GATC 1 cut(s) 448
BssNI GRCGYC 3 cut(s) 5, 72, 432
BssT1I CCWWGG 3 cut(s) 256, 310, 418
Bst2UI CCWGG 3 cut(s) 295, 482, 581
Bst4CI ACNGT 7 cut(s) 28, 555, 594, 792, 803, 844, 930
BstACI GRCGYC 3 cut(s) 5, 72, 432
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 1 cut(s) 705
BstDSI CCRYGG 2 cut(s) 27, 310
BstF5I GGATG 3 cut(s) 139, 715, 826
BstKTI GATC 1 cut(s) 451
BstMAI GTCTC 1 cut(s) 726
BstMBI GATC 1 cut(s) 448
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 6 cut(s) 261, 270, 379, 613, 710, 781
BstNI CCWGG 3 cut(s) 295, 482, 581
BstSCI CCNGG 4 cut(s) 293, 480, 579, 607
BstSLI GKGCMC 2 cut(s) 408, 737
BstV1I GCAGC 2 cut(s) 151, 600
BsuI GTATCC 1 cut(s) 865
BsuRI GGCC 5 cut(s) 361, 406, 416, 564, 837
BtgI CCRYGG 2 cut(s) 27, 310
BtsCI GGATG 3 cut(s) 139, 715, 826
BtsIMutI CAGTG 3 cut(s) 737, 799, 840
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 618
Cfr10I RCCGGY 2 cut(s) 195, 694
Cfr13I GGNCC 4 cut(s) 404, 405, 415, 551
Csp6I GTAC 2 cut(s) 496, 778
CspAI ACCGGT 1 cut(s) 195
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviAII CATG 4 cut(s) 247, 311, 388, 538
CviQI GTAC 2 cut(s) 496, 778
DdeI CTNAG 1 cut(s) 705
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
DraI TTTAAA 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 359
EciI GGCGGA 1 cut(s) 929
Eco130I CCWWGG 3 cut(s) 256, 310, 418
Eco24I GRGCYC 1 cut(s) 408
Eco47I GGWCC 1 cut(s) 551
Eco88I CYCGRG 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 405
EcoRII CCWGG 3 cut(s) 293, 480, 579
EcoT14I CCWWGG 3 cut(s) 256, 310, 418
EcoT38I GRGCYC 1 cut(s) 408
ErhI CCWWGG 3 cut(s) 256, 310, 418
Esp3I CGTCTC 1 cut(s) 726
FaeI CATG 4 cut(s) 250, 314, 391, 541
FaiI YATR 6 cut(s) 248, 312, 389, 527, 539, 908
FaqI GGGAC 2 cut(s) 564, 724
FatI CATG 4 cut(s) 246, 310, 387, 537
FauI CCCGC 3 cut(s) 269, 320, 592
FblI GTMKAC 2 cut(s) 192, 435
Fnu4HI GCNGC 3 cut(s) 165, 274, 614
FokI GGATG 3 cut(s) 126, 722, 813
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 3 cut(s) 165, 274, 614
GluI GCNGC 3 cut(s) 165, 274, 614
HaeIII GGCC 5 cut(s) 361, 406, 416, 564, 837
HapII CCGG 6 cut(s) 159, 196, 362, 413, 608, 695
Hin1I GRCGYC 3 cut(s) 5, 72, 432
Hin1II CATG 4 cut(s) 250, 314, 391, 541
HincII GTYRAC 2 cut(s) 193, 436
HindII GTYRAC 2 cut(s) 193, 436
HinfI GANTC 3 cut(s) 365, 820, 898
HpaII CCGG 6 cut(s) 159, 196, 362, 413, 608, 695
HphI GGTGA 2 cut(s) 684, 829
Hpy166II GTNNAC 4 cut(s) 37, 193, 436, 498
Hpy188I TCNGA 5 cut(s) 230, 320, 430, 674, 903
Hpy188III TCNNGA 5 cut(s) 376, 398, 518, 661, 769
Hpy8I GTNNAC 4 cut(s) 37, 193, 436, 498
Hpy99I CGWCG 7 cut(s) 74, 77, 194, 434, 437, 440, 563
HpyAV CCTTC 3 cut(s) 263, 616, 752
HpyCH4III ACNGT 7 cut(s) 28, 555, 594, 792, 803, 844, 930
HpyCH4IV ACGT 3 cut(s) 5, 72, 432
HpyCH4V TGCA 1 cut(s) 616
HpyF10VI GCNNNNNNNGC 6 cut(s) 261, 270, 379, 613, 710, 781
HpyF3I CTNAG 1 cut(s) 705
HpySE526I ACGT 3 cut(s) 5, 72, 432
Hsp92I GRCGYC 3 cut(s) 5, 72, 432
Hsp92II CATG 4 cut(s) 250, 314, 391, 541
Kzo9I GATC 1 cut(s) 448
LmnI GCTCC 4 cut(s) 109, 161, 252, 584
Lsp1109I GCAGC 2 cut(s) 151, 600
LweI GCATC 2 cut(s) 700, 799
MaeII ACGT 3 cut(s) 5, 72, 432
MaeIII GTNAC 1 cut(s) 690
MalI GATC 1 cut(s) 450
MbiI CCGCTC 1 cut(s) 327
MboI GATC 1 cut(s) 448
MboII GAAGA 3 cut(s) 184, 201, 542
MhlI GDGCHC 3 cut(s) 375, 408, 737
MluCI AATT 7 cut(s) 44, 123, 473, 485, 651, 656, 683
MmeI TCCRAC 3 cut(s) 121, 453, 774
MseI TTAA 3 cut(s) 111, 543, 654
MspI CCGG 6 cut(s) 159, 196, 362, 413, 608, 695
MspR9I CCNGG 4 cut(s) 295, 482, 581, 609
Mva1269I GAATGC 1 cut(s) 134
MvaI CCWGG 3 cut(s) 295, 482, 581
MwoI GCNNNNNNNGC 6 cut(s) 261, 270, 379, 613, 710, 781
NciI CCSGG 1 cut(s) 609
NcoI CCATGG 1 cut(s) 310
NdeII GATC 1 cut(s) 448
NlaIII CATG 4 cut(s) 250, 314, 391, 541
NlaIV GGNNCC 5 cut(s) 254, 317, 406, 470, 552
NmeAIII GCCGAG 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 690
PaeR7I CTCGAG 1 cut(s) 374
PasI CCCWGGG 1 cut(s) 580
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PctI GAATGC 1 cut(s) 134
PfeI GAWTC 3 cut(s) 365, 820, 898
Pfl23II CGTACG 1 cut(s) 777
PflFI GACNNNGTC 1 cut(s) 74
PinAI ACCGGT 1 cut(s) 195
PkrI GCNGC 3 cut(s) 166, 275, 615
Psp6I CCWGG 3 cut(s) 293, 480, 579
PspGI CCWGG 3 cut(s) 293, 480, 579
PspLI CGTACG 1 cut(s) 777
PspN4I GGNNCC 5 cut(s) 254, 317, 406, 470, 552
PspOMI GGGCCC 1 cut(s) 404
PspPI GGNCC 4 cut(s) 404, 405, 415, 551
PsyI GACNNNGTC 1 cut(s) 74
RsaI GTAC 2 cut(s) 497, 779
RsaNI GTAC 2 cut(s) 496, 778
SalI GTCGAC 2 cut(s) 191, 434
SaqAI TTAA 3 cut(s) 111, 543, 654
SatI GCNGC 3 cut(s) 165, 274, 614
Sau3AI GATC 1 cut(s) 448
Sau96I GGNCC 4 cut(s) 404, 405, 415, 551
ScrFI CCNGG 4 cut(s) 295, 482, 581, 609
SduI GDGCHC 3 cut(s) 375, 408, 737
SetI ASST 8 cut(s) 8, 75, 103, 166, 299, 384, 424, 435
SfaNI GCATC 2 cut(s) 700, 799
Sfr274I CTCGAG 1 cut(s) 374
SgrAI CRCCGGYG 1 cut(s) 694
SgrDI CGTCGACG 1 cut(s) 434
SinI GGWCC 1 cut(s) 551
SlaI CTCGAG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 7 cut(s) 44, 123, 473, 485, 651, 656, 683
SsiI CCGC 7 cut(s) 264, 273, 276, 327, 599, 698, 914
SspI AATATT 1 cut(s) 206
StyD4I CCNGG 4 cut(s) 293, 480, 579, 607
StyI CCWWGG 3 cut(s) 256, 310, 418
TaaI ACNGT 7 cut(s) 28, 555, 594, 792, 803, 844, 930
TaiI ACGT 3 cut(s) 8, 75, 435
TaqI TCGA 8 cut(s) 192, 284, 375, 384, 399, 435, 558, 891
TaqII GACCGA 1 cut(s) 66
TasI AATT 7 cut(s) 44, 123, 473, 485, 651, 656, 683
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 3 cut(s) 365, 820, 898
Tru1I TTAA 3 cut(s) 111, 543, 654
Tru9I TTAA 3 cut(s) 111, 543, 654
TscAI CASTG 3 cut(s) 737, 806, 847
TseFI GTSAC 1 cut(s) 690
TseI GCWGC 2 cut(s) 164, 613
Tsp45I GTSAC 1 cut(s) 690
TspDTI ATGAA 4 cut(s) 812, 873, 877, 896
TspRI CASTG 3 cut(s) 737, 806, 847
Tth111I GACNNNGTC 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 551
XapI RAATTY 4 cut(s) 44, 485, 656, 683
XhoI CTCGAG 1 cut(s) 374
XmiI GTMKAC 2 cut(s) 192, 435
ZraI GACGTC 3 cut(s) 6, 73, 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.