pycom16g07800

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003359
Physical Location & Seq
Forward (+)
5143547 .. 5145607
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2061 bp
ATGATTCAGAGATCAAACGGGTCAGGTCGCCGGATTCTGACGTACCCGGCGGTTCATCCGTGCGAAGTCATATCTCCGCAGACACTCCTGGCGTCACTCATCACTCTCACCTGCAACATTTGCTCTTTCAAGTCGAAAGTCATCGCCAGCAACGCCCGAAACGCCCGAGAAATCATCCGACAAATCGGTGTCGTCCGGGTTTTTCTCGAAGCGGTTCGAGATGAATCGTCGGGTCTTCCGACCTCCGCAGTTCTGAGCTTGTCGGAGCTCCACTTGGCCTTCCAGAAAGTTCAGTTCTTGCTGGAAGACTTGGCCCGGGACGACGCCAGGGTATGGATGCTGATGAAGTCGGGTCACGTGGCGAGTCAGTTTAGGATCTTGAGTCGGGCCATGGGCACGGCTCTCGATGTTCTGCCTCTCGCGTCGGTCGACGCCTCCGTTGAGGTCAAGGAGCACGTGGAATTGGTGGCCAGGCAAGCGCGAAAGACGGGGTATGAAGTCGACCCGGATGATATTCGTGTCGTGGAGGCGGTGAGGTCCGTTTTGGCCGGGTTAGAGAAAGGAGTTGCTCCGGACAAGGGGGATTTGAAATGGGTTCTCGAGTTCCTCGGGATTAGGAAATGGAGCAGGTGTGAAAAGCAAGTCAAGTTCTTGGATTCGGAAATTGGGTTCGAGAATTTGAGCCAGGAGAAGAGAGAGGTGGGATTGTTGTGCAGCCTGAAGGGTTTGCTTTGCTACTGTCGATGTGTGTTGTTCGATGTTGTGGATGGTGAACCGGGTCGAAGATCCGAGACCAAATGCAGCGGCGCCGGCGTGGCGGTGGATTGCCTCAATTCGGAAGATTTCCGGTGCCCGATATCGCTGGAAATCATGGCTGATCCGGTCACGATAGCAACAGGGCAGAGCTACGATCGGTCTTCGATTGAGAAATGGTTCAGAGCTGGCAATCCCATTTGTCCCAAGACGGGGGAGAAGCTCAAGAACACGGAGGTGGTGCCGAATTTGGCGCTAAAGCGATTGATCCAGCAGTATTGTTGCGAAAATGGTGTTGCATTTGCTGATTCGGGTCACCGAAATCACGACAGTGGGAGGACTAGGCTTGCAGTTGCTGGGAGTTTGGCAGCTGAGGGAGCCATGAAAATGGTGGCTGAGTTTCTTAATGGATGGCTTGAGGTTGGGACAGTAGAAGAGCAGAACAAGGCCGCTTATGAAATCCGGCTTCTTTCGAAATCAAGCATTTTTAACAGGTCTTGTTTGTTTGAAGCCGGTGTGGTTCCACATTTGTTGAAACTGTTATGTTCAGCAGATTCAGGGATTCAAGAAAATGCCATCGCCTCCTTGTTGAATCTTTCGAAGTATACGAAAAGCAAGGCTGTGATTGTCGAAAATCGTGGTCTGGATTTGATTGTAGGTGTGGTGAAGAAAGGTCTCAGATTAGAGGCTCGTCAGCATGCTGCTGGCACATTGTTTTACCTCTCTTCGGTCGAGGAGTACAGGAGATTGATTGGAGAAAATGCAGATGCCATACCGGCATTGATGGAGTTGATCAAGGATGGAACTGATAGGGCCAAGAAAAACGCATTGGTTGCGATTTTCGGGCTCATTAATCACCCGGAAAATCATAGGAGGGTGATAGTAGCAGGGGTAGTTCCTCTGCTTGTTAATGTCTTAACATCATTTGATAATGAAGCTCTCATCACAGATGCTCTGGCAATTCTTTCAACTCTCGCAGAGAAACCTGATGGATCAGTTGCAATTTCGCGTTGCAAAGCTTTGGATACGATCGTAGGGATCCTGAATTCCTCCATTCCTAGGGCAGGGAAGGAGTTCTGCGTGTCTTTGTTGCTTGCTCTTTGCGTAAATGGCAGCGGAGATGTGGTTGCTCTTCTAGCAAAGAGCCCTTCACTTATGGGACCTTTGTATTCTCAACTCAACGAAGGCACGTCTCGAGCAAGCAAAAAGGCCAGTGCTCTCATCAAGGTCCTGCATGAGTTCCATGAAAGAAGGTCATCCGGCTCAATCTCCCCAGTTCTACAAGAGCGATTCGTTCACGTTAGGTAA

Protein Analysis

687

Amino Acids

74.79

Weight (kDa)

8.5

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB10_N PF25368 57 - 161 1.2e-09 PUB10-like, N-terminal domain
U-box PF04564 280 - 348 3e-18 U-box domain
ARM_ARMC5 PF24768 371 - 525 3.1e-07 ARMC5
ARM_PUB PF25598 397 - 619 5.3e-15 Plant U-box protein ARM repeat region
Arm PF00514 415 - 451 8.3e-08 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 119, 618
Acc36I ACCTGC 2 cut(s) 119, 618
AccB1I GGYRCC 3 cut(s) 806, 849, 994
AccB7I CCANNNNNTGG 1 cut(s) 333
AccI GTMKAC 3 cut(s) 429, 501, 1358
AccII CGCG 3 cut(s) 422, 481, 1762
AccIII TCCGGA 1 cut(s) 571
AciI CCGC 9 cut(s) 50, 77, 212, 246, 530, 804, 818, 1203, 1869
AclWI GGATC 7 cut(s) 383, 780, 872, 1015, 1753, 1786, 1799
AcoI YGGCCR 2 cut(s) 468, 546
AcsI RAATTY 3 cut(s) 676, 1000, 1798
AcuI CTGAAG 1 cut(s) 740
AcvI CACGTG 2 cut(s) 358, 457
AcyI GRCGYC 4 cut(s) 92, 324, 432, 807
AfaI GTAC 2 cut(s) 44, 1493
AfiI CCNNNNNNNGG 8 cut(s) 333, 578, 835, 965, 966, 1480, 1812, 1817
AgsI TTSAA 7 cut(s) 130, 589, 1262, 1288, 1319, 1345, 1722
AjiI CACGTC 1 cut(s) 1944
AjnI CCWGG 4 cut(s) 87, 326, 470, 684
AjuI GAANNNNNNNTTGG 2 cut(s) 1565, 1597
AleI CACNNNNGTG 2 cut(s) 989, 1083
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 8 cut(s) 258, 268, 906, 941, 976, 1124, 1691, 1772
AluI AGCT 8 cut(s) 258, 268, 906, 941, 976, 1124, 1691, 1772
Alw21I GWGCWC 3 cut(s) 270, 456, 1972
Alw26I GTCTC 3 cut(s) 785, 1433, 1950
AlwI GGATC 7 cut(s) 383, 780, 872, 1015, 1753, 1786, 1799
AlwNI CAGNNNCTG 1 cut(s) 1109
Ama87I CYCGRG 5 cut(s) 165, 315, 599, 608, 1947
Aor13HI TCCGGA 1 cut(s) 571
AoxI GGCC 8 cut(s) 276, 312, 387, 468, 546, 1200, 1566, 1962
ApeKI GCWGC 5 cut(s) 714, 801, 1121, 1454, 1866
ApoI RAATTY 3 cut(s) 676, 1000, 1798
AseI ATTAAT 1 cut(s) 1605
Asp700I GAANNNNTTC 3 cut(s) 213, 932, 1826
AspA2I CCTAGG 1 cut(s) 1811
AspLEI GCGC 3 cut(s) 481, 809, 1009
AspS9I GGNCC 6 cut(s) 313, 387, 537, 1566, 1913, 1981
AsuC2I CCSGG 8 cut(s) 47, 197, 316, 317, 506, 550, 777, 1613
AsuHPI GGTGA 7 cut(s) 100, 544, 782, 1061, 1429, 1601, 1642
AsuII TTCGAA 2 cut(s) 1226, 1352
AvaI CYCGRG 5 cut(s) 165, 315, 599, 608, 1947
AvaII GGWCC 3 cut(s) 537, 1913, 1981
AvrII CCTAGG 1 cut(s) 1811
BaeGI GKGCMC 2 cut(s) 398, 854
BaeI ACNNNNGTAYC 2 cut(s) 1770, 1803
BalI TGGCCA 1 cut(s) 470
BamHI GGATCC 1 cut(s) 1791
BanI GGYRCC 3 cut(s) 806, 849, 994
BanII GRGCYC 3 cut(s) 270, 1602, 1901
BbrPI CACGTG 2 cut(s) 358, 457
BbsI GAAGAC 3 cut(s) 227, 312, 909
Bbv12I GWGCWC 3 cut(s) 270, 456, 1972
BbvCI CCTCAGC 1 cut(s) 1125
BbvI GCAGC 5 cut(s) 726, 813, 1133, 1441, 1878
BccI CCATC 6 cut(s) 761, 1158, 1337, 1531, 1547, 1736
BceAI ACGGC 1 cut(s) 414
BcgI CGANNNNNNTGC 2 cut(s) 385, 419
BciT130I CCWGG 4 cut(s) 89, 328, 472, 686
BciVI GTATCC 1 cut(s) 1771
BclI TGATCA 1 cut(s) 1545
BcnI CCSGG 8 cut(s) 47, 197, 316, 317, 506, 550, 777, 1613
BcoDI GTCTC 3 cut(s) 785, 1433, 1950
BfaI CTAG 3 cut(s) 1095, 1812, 1889
BfoI RGCGCY 2 cut(s) 810, 1010
BfuAI ACCTGC 2 cut(s) 119, 618
BfuI GTATCC 1 cut(s) 1771
BglI GCCNNNNNGGC 2 cut(s) 815, 1529
BisI GCNGC 7 cut(s) 715, 802, 805, 1122, 1203, 1455, 1867
BlnI CCTAGG 1 cut(s) 1811
BlsI GCNGC 7 cut(s) 716, 803, 806, 1123, 1204, 1456, 1868
Bme18I GGWCC 3 cut(s) 537, 1913, 1981
BmeT110I CYCGRG 5 cut(s) 165, 315, 599, 608, 1947
BmgBI CACGTC 1 cut(s) 1944
BmgT120I GGNCC 6 cut(s) 313, 387, 537, 1566, 1913, 1981
BmiI GGNNCC 7 cut(s) 808, 851, 996, 1132, 1275, 1793, 1914
BmrI ACTGGG 1 cut(s) 2021
BmsI GCATC 3 cut(s) 327, 1510, 1693
BmuI ACTGGG 1 cut(s) 2021
BpiI GAAGAC 3 cut(s) 227, 312, 909
Bpu10I CCTNAGC 1 cut(s) 1125
Bpu14I TTCGAA 2 cut(s) 1226, 1352
BpuEI CTTGAG 3 cut(s) 400, 962, 1190
BpuMI CCSGG 8 cut(s) 47, 197, 316, 317, 506, 550, 777, 1613
BsaAI YACGTR 2 cut(s) 358, 457
BsaHI GRCGYC 4 cut(s) 92, 324, 432, 807
BsaI GGTCTC 2 cut(s) 785, 1433
BsaJI CCNNGG 5 cut(s) 315, 327, 390, 607, 1811
BsaWI WCCGGW 3 cut(s) 571, 846, 880
BsaXI ACNNNNNCTCC 4 cut(s) 616, 646, 1863, 1893
Bsc4I CCNNNNNNNGG 8 cut(s) 333, 578, 835, 965, 966, 1480, 1812, 1817
Bse118I RCCGGY 3 cut(s) 809, 1265, 1528
Bse1I ACTGG 2 cut(s) 1965, 2027
BseAI TCCGGA 1 cut(s) 571
BseBI CCWGG 4 cut(s) 89, 328, 472, 686
BseDI CCNNGG 5 cut(s) 315, 327, 390, 607, 1811
BseGI GGATG 8 cut(s) 55, 174, 342, 514, 772, 1169, 1558, 2009
BseLI CCNNNNNNNGG 8 cut(s) 333, 578, 835, 965, 966, 1480, 1812, 1817
BseMII CTCAG 4 cut(s) 245, 1116, 1140, 1444
BseNI ACTGG 2 cut(s) 1965, 2027
BseRI GAGGAG 1 cut(s) 1502
BseSI GKGCMC 2 cut(s) 398, 854
BseXI GCAGC 5 cut(s) 726, 813, 1133, 1441, 1878
BseYI CCCAGC 1 cut(s) 1109
BsgI GTGCAG 1 cut(s) 733
Bsh1236I CGCG 3 cut(s) 422, 481, 1762
Bsh1285I CGRYCG 4 cut(s) 429, 913, 1485, 1785
BshFI GGCC 8 cut(s) 278, 314, 389, 470, 548, 1202, 1568, 1964
BshNI GGYRCC 3 cut(s) 806, 849, 994
BsiEI CGRYCG 4 cut(s) 429, 913, 1485, 1785
BsiHKAI GWGCWC 3 cut(s) 270, 456, 1972
BsiHKCI CYCGRG 5 cut(s) 165, 315, 599, 608, 1947
BslFI GGGAC 4 cut(s) 332, 942, 1192, 1926
BslI CCNNNNNNNGG 8 cut(s) 333, 578, 835, 965, 966, 1480, 1812, 1817
BsmAI GTCTC 3 cut(s) 785, 1433, 1950
BsmBI CGTCTC 1 cut(s) 1950
BsmFI GGGAC 4 cut(s) 332, 942, 1192, 1926
BsnI GGCC 8 cut(s) 278, 314, 389, 470, 548, 1202, 1568, 1964
Bso31I GGTCTC 2 cut(s) 785, 1433
BsoBI CYCGRG 5 cut(s) 165, 315, 599, 608, 1947
Bsp119I TTCGAA 2 cut(s) 1226, 1352
Bsp1286I GDGCHC 7 cut(s) 270, 398, 456, 854, 1602, 1901, 1972
Bsp13I TCCGGA 1 cut(s) 571
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 9 cut(s) 50, 77, 212, 246, 530, 804, 818, 1203, 1869
BspANI GGCC 8 cut(s) 278, 314, 389, 470, 548, 1202, 1568, 1964
BspCNI CTCAG 4 cut(s) 246, 1117, 1141, 1443
BspEI TCCGGA 1 cut(s) 571
BspFNI CGCG 3 cut(s) 422, 481, 1762
BspLI GGNNCC 7 cut(s) 808, 851, 996, 1132, 1275, 1793, 1914
BspMI ACCTGC 2 cut(s) 119, 618
BspPI GGATC 7 cut(s) 383, 780, 872, 1015, 1753, 1786, 1799
BspQI GCTCTTC 2 cut(s) 1182, 1890
BspT104I TTCGAA 2 cut(s) 1226, 1352
BspT107I GGYRCC 3 cut(s) 806, 849, 994
BspTNI GGTCTC 2 cut(s) 785, 1433
BsrFI RCCGGY 3 cut(s) 809, 1265, 1528
BsrI ACTGG 2 cut(s) 1965, 2027
BssAI RCCGGY 3 cut(s) 809, 1265, 1528
BssECI CCNNGG 5 cut(s) 315, 327, 390, 607, 1811
BssNAI GTATAC 1 cut(s) 1359
BssNI GRCGYC 4 cut(s) 92, 324, 432, 807
BssT1I CCWWGG 2 cut(s) 390, 1811
Bst1107I GTATAC 1 cut(s) 1359
Bst2UI CCWGG 4 cut(s) 89, 328, 472, 686
Bst4CI ACNGT 4 cut(s) 740, 1085, 1183, 1293
Bst6I CTCTTC 4 cut(s) 686, 1182, 1483, 1890
BstACI GRCGYC 4 cut(s) 92, 324, 432, 807
BstAPI GCANNNNNTGC 2 cut(s) 120, 1586
BstBAI YACGTR 2 cut(s) 358, 457
BstBI TTCGAA 2 cut(s) 1226, 1352
BstC8I GCNNGC 9 cut(s) 148, 477, 811, 943, 1101, 1452, 1459, 1848, 1954
BstDEI CTNAG 4 cut(s) 254, 1125, 1149, 1430
BstDSI CCRYGG 1 cut(s) 390
BstEII GGTNACC 1 cut(s) 1067
BstENI CCTNNNNNAGG 1 cut(s) 1815
BstF5I GGATG 8 cut(s) 55, 174, 342, 514, 772, 1169, 1558, 2009
BstFNI CGCG 3 cut(s) 422, 481, 1762
BstH2I RGCGCY 2 cut(s) 810, 1010
BstHHI GCGC 3 cut(s) 481, 809, 1009
BstMAI GTCTC 3 cut(s) 785, 1433, 1950
BstMCI CGRYCG 4 cut(s) 429, 913, 1485, 1785
BstNI CCWGG 4 cut(s) 89, 328, 472, 686
BstNSI RCATGY 1 cut(s) 1454
BstPI GGTNACC 1 cut(s) 1067
BstSLI GKGCMC 2 cut(s) 398, 854
BstUI CGCG 3 cut(s) 422, 481, 1762
BstV1I GCAGC 5 cut(s) 726, 813, 1133, 1441, 1878
BstV2I GAAGAC 3 cut(s) 227, 312, 909
BstX2I RGATCY 3 cut(s) 375, 785, 1791
BstXI CCANNNNNNTGG 1 cut(s) 1141
BstYI RGATCY 3 cut(s) 375, 785, 1791
BstZ17I GTATAC 1 cut(s) 1359
BsuI GTATCC 1 cut(s) 1771
BsuRI GGCC 8 cut(s) 278, 314, 389, 470, 548, 1202, 1568, 1964
BtgI CCRYGG 1 cut(s) 390
BtgZI GCGATG 2 cut(s) 127, 1315
BtrI CACGTC 1 cut(s) 1944
BtsCI GGATG 8 cut(s) 55, 174, 342, 514, 772, 1169, 1558, 2009
BtsIMutI CAGTG 2 cut(s) 1090, 1972
BveI ACCTGC 2 cut(s) 119, 618
Cac8I GCNNGC 9 cut(s) 148, 477, 811, 943, 1101, 1452, 1459, 1848, 1954
CaiI CAGNNNCTG 1 cut(s) 1109
CfoI GCGC 3 cut(s) 481, 809, 1009
Cfr10I RCCGGY 3 cut(s) 809, 1265, 1528
Cfr13I GGNCC 6 cut(s) 313, 387, 537, 1566, 1913, 1981
Cfr9I CCCGGG 1 cut(s) 315
CseI GACGC 4 cut(s) 81, 332, 411, 440
Csp6I GTAC 2 cut(s) 43, 1492
CspCI CAANNNNNGTGG 2 cut(s) 1266, 1301
CviAII CATG 6 cut(s) 391, 871, 1135, 1451, 1988, 1997
CviQI GTAC 2 cut(s) 43, 1492
DdeI CTNAG 4 cut(s) 254, 1125, 1149, 1430
DinI GGCGCC 1 cut(s) 808
EaeI YGGCCR 2 cut(s) 468, 546
Eam1104I CTCTTC 4 cut(s) 686, 1182, 1483, 1890
EarI CTCTTC 4 cut(s) 686, 1182, 1483, 1890
Ecl136II GAGCTC 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 390, 1811
Eco24I GRGCYC 3 cut(s) 270, 1602, 1901
Eco31I GGTCTC 2 cut(s) 785, 1433
Eco32I GATATC 1 cut(s) 858
Eco47I GGWCC 3 cut(s) 537, 1913, 1981
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 740
Eco72I CACGTG 2 cut(s) 358, 457
Eco88I CYCGRG 5 cut(s) 165, 315, 599, 608, 1947
Eco91I GGTNACC 1 cut(s) 1067
EcoICRI GAGCTC 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 1815
EcoO109I RGGNCCY 2 cut(s) 1913, 1981
EcoO65I GGTNACC 1 cut(s) 1067
EcoRI GAATTC 1 cut(s) 1798
EcoRII CCWGG 4 cut(s) 87, 326, 470, 684
EcoRV GATATC 1 cut(s) 858
EcoT14I CCWWGG 2 cut(s) 390, 1811
EcoT38I GRGCYC 3 cut(s) 270, 1602, 1901
EgeI GGCGCC 1 cut(s) 808
EheI GGCGCC 1 cut(s) 808
ErhI CCWWGG 2 cut(s) 390, 1811
Esp3I CGTCTC 1 cut(s) 1950
FaeI CATG 6 cut(s) 394, 874, 1138, 1454, 1991, 2000
FaqI GGGAC 4 cut(s) 332, 942, 1192, 1926
FatI CATG 6 cut(s) 390, 870, 1134, 1450, 1987, 1996
FbaI TGATCA 1 cut(s) 1545
FblI GTMKAC 3 cut(s) 429, 501, 1358
Fnu4HI GCNGC 7 cut(s) 715, 802, 805, 1122, 1203, 1455, 1867
FokI GGATG 8 cut(s) 42, 161, 349, 521, 779, 1176, 1565, 1996
FriOI GRGCYC 3 cut(s) 270, 1602, 1901
Fsp4HI GCNGC 7 cut(s) 715, 802, 805, 1122, 1203, 1455, 1867
FspBI CTAG 3 cut(s) 1095, 1812, 1889
GlaI GCGC 3 cut(s) 480, 808, 1008
GluI GCNGC 7 cut(s) 715, 802, 805, 1122, 1203, 1455, 1867
GsaI CCCAGC 1 cut(s) 1113
HaeII RGCGCY 2 cut(s) 810, 1010
HaeIII GGCC 8 cut(s) 278, 314, 389, 470, 548, 1202, 1568, 1964
HgaI GACGC 4 cut(s) 81, 332, 411, 440
HhaI GCGC 3 cut(s) 481, 809, 1009
Hin1I GRCGYC 4 cut(s) 92, 324, 432, 807
Hin1II CATG 6 cut(s) 394, 874, 1138, 1454, 1991, 2000
Hin6I GCGC 3 cut(s) 479, 807, 1007
HinP1I GCGC 3 cut(s) 479, 807, 1007
HincII GTYRAC 2 cut(s) 430, 502
HindII GTYRAC 2 cut(s) 430, 502
HindIII AAGCTT 1 cut(s) 1770
HphI GGTGA 7 cut(s) 100, 544, 782, 1061, 1429, 1601, 1642
Hpy166II GTNNAC 5 cut(s) 430, 502, 773, 1359, 2050
Hpy8I GTNNAC 5 cut(s) 430, 502, 773, 1359, 2050
Hpy99I CGWCG 4 cut(s) 232, 326, 427, 434
HpyAV CCTTC 6 cut(s) 289, 715, 1816, 1911, 1931, 1998
HpyCH4III ACNGT 4 cut(s) 740, 1085, 1183, 1293
HpyCH4IV ACGT 5 cut(s) 41, 357, 456, 1943, 2052
HpyCH4V TGCA 9 cut(s) 114, 714, 801, 1052, 1103, 1517, 1754, 1767, 1987
HpyF3I CTNAG 4 cut(s) 254, 1125, 1149, 1430
HpySE526I ACGT 5 cut(s) 41, 357, 456, 1943, 2052
Hsp92I GRCGYC 4 cut(s) 92, 324, 432, 807
Hsp92II CATG 6 cut(s) 394, 874, 1138, 1454, 1991, 2000
HspAI GCGC 3 cut(s) 479, 807, 1007
KasI GGCGCC 1 cut(s) 806
Kpn2I TCCGGA 1 cut(s) 571
KroI GCCGGC 1 cut(s) 809
KroNI GCCGGC 1 cut(s) 811
Ksp22I TGATCA 1 cut(s) 1545
LguI GCTCTTC 2 cut(s) 1182, 1890
LmnI GCTCC 6 cut(s) 265, 273, 451, 574, 624, 1130
Lsp1109I GCAGC 5 cut(s) 726, 813, 1133, 1441, 1878
LweI GCATC 3 cut(s) 327, 1510, 1693
MaeI CTAG 3 cut(s) 1095, 1812, 1889
MaeII ACGT 5 cut(s) 41, 357, 456, 1943, 2052
MaeIII GTNAC 4 cut(s) 93, 353, 883, 1067
MflI RGATCY 3 cut(s) 375, 785, 1791
MhlI GDGCHC 7 cut(s) 270, 398, 456, 854, 1602, 1901, 1972
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 8 cut(s) 461, 663, 676, 832, 1000, 1713, 1755, 1798
MluNI TGGCCA 1 cut(s) 470
Mly113I GGCGCC 1 cut(s) 807
MlyI GAGTC 2 cut(s) 373, 391
MmeI TCCRAC 3 cut(s) 202, 243, 263
Mox20I TGGCCA 1 cut(s) 470
MreI CGCCGGCG 1 cut(s) 809
MroI TCCGGA 1 cut(s) 571
MroNI GCCGGC 1 cut(s) 809
MroXI GAANNNNTTC 3 cut(s) 213, 932, 1826
MscI TGGCCA 1 cut(s) 470
MseI TTAA 5 cut(s) 1158, 1242, 1605, 1662, 1670
MslI CAYNNNNRTG 3 cut(s) 989, 1083, 1139
Msp20I TGGCCA 1 cut(s) 470
MspA1I CMGCKG 3 cut(s) 804, 1124, 1869
MvaI CCWGG 4 cut(s) 89, 328, 472, 686
MvnI CGCG 3 cut(s) 422, 481, 1762
NaeI GCCGGC 1 cut(s) 811
NarI GGCGCC 1 cut(s) 807
NciI CCSGG 8 cut(s) 47, 197, 316, 317, 506, 550, 777, 1613
NcoI CCATGG 1 cut(s) 390
NgoMIV GCCGGC 1 cut(s) 809
NlaIII CATG 6 cut(s) 394, 874, 1138, 1454, 1991, 2000
NlaIV GGNNCC 7 cut(s) 808, 851, 996, 1132, 1275, 1793, 1914
NmuCI GTSAC 4 cut(s) 93, 353, 883, 1067
NspI RCATGY 1 cut(s) 1454
NspV TTCGAA 2 cut(s) 1226, 1352
OliI CACNNNNGTG 2 cut(s) 989, 1083
PaeI GCATGC 1 cut(s) 1454
PaeR7I CTCGAG 2 cut(s) 599, 1947
PaqCI CACCTGC 2 cut(s) 119, 618
PciSI GCTCTTC 2 cut(s) 1182, 1890
PcsI WCGNNNNNNNCGW 4 cut(s) 236, 426, 435, 1358
PdiI GCCGGC 1 cut(s) 811
PdmI GAANNNNTTC 3 cut(s) 213, 932, 1826
PfeI GAWTC 9 cut(s) 4, 34, 224, 656, 1061, 1307, 1315, 1345, 2043
PflMI CCANNNNNTGG 1 cut(s) 333
PkrI GCNGC 7 cut(s) 716, 803, 806, 1123, 1204, 1456, 1868
Ple19I CGATCG 2 cut(s) 913, 1785
PleI GAGTC 2 cut(s) 372, 390
PluTI GGCGCC 1 cut(s) 810
PmaCI CACGTG 2 cut(s) 358, 457
PmlI CACGTG 2 cut(s) 358, 457
PpsI GAGTC 2 cut(s) 372, 390
Ppu21I YACGTR 2 cut(s) 358, 457
PpuMI RGGWCCY 2 cut(s) 1913, 1981
PshBI ATTAAT 1 cut(s) 1605
Psp124BI GAGCTC 1 cut(s) 270
Psp5II RGGWCCY 2 cut(s) 1913, 1981
Psp6I CCWGG 4 cut(s) 87, 326, 470, 684
PspCI CACGTG 2 cut(s) 358, 457
PspEI GGTNACC 1 cut(s) 1067
PspFI CCCAGC 1 cut(s) 1109
PspGI CCWGG 4 cut(s) 87, 326, 470, 684
PspN4I GGNNCC 7 cut(s) 808, 851, 996, 1132, 1275, 1793, 1914
PspPI GGNCC 6 cut(s) 313, 387, 537, 1566, 1913, 1981
PspPPI RGGWCCY 2 cut(s) 1913, 1981
PstNI CAGNNNCTG 1 cut(s) 1109
PsuI RGATCY 3 cut(s) 375, 785, 1791
PvuI CGATCG 2 cut(s) 913, 1785
PvuII CAGCTG 1 cut(s) 1124
RsaI GTAC 2 cut(s) 44, 1493
RsaNI GTAC 2 cut(s) 43, 1492
RseI CAYNNNNRTG 3 cut(s) 989, 1083, 1139
SacI GAGCTC 1 cut(s) 270
SalI GTCGAC 2 cut(s) 428, 500
SapI GCTCTTC 2 cut(s) 1182, 1890
SaqAI TTAA 5 cut(s) 1158, 1242, 1605, 1662, 1670
SatI GCNGC 7 cut(s) 715, 802, 805, 1122, 1203, 1455, 1867
Sau96I GGNCC 6 cut(s) 313, 387, 537, 1566, 1913, 1981
SchI GAGTC 2 cut(s) 373, 391
SduI GDGCHC 7 cut(s) 270, 398, 456, 854, 1602, 1901, 1972
SfaNI GCATC 3 cut(s) 327, 1510, 1693
SfoI GGCGCC 1 cut(s) 808
Sfr274I CTCGAG 2 cut(s) 599, 1947
SfuI TTCGAA 2 cut(s) 1226, 1352
SgrAI CRCCGGYG 1 cut(s) 809
SinI GGWCC 3 cut(s) 537, 1913, 1981
SlaI CTCGAG 2 cut(s) 599, 1947
SmaI CCCGGG 1 cut(s) 317
SmiMI CAYNNNNRTG 3 cut(s) 989, 1083, 1139
SmlI CTYRAG 5 cut(s) 379, 599, 977, 1169, 1947
SmoI CTYRAG 5 cut(s) 379, 599, 977, 1169, 1947
SphI GCATGC 1 cut(s) 1454
Sse9I AATT 8 cut(s) 461, 663, 676, 832, 1000, 1713, 1755, 1798
SsiI CCGC 9 cut(s) 50, 77, 212, 246, 530, 804, 818, 1203, 1869
SspDI GGCGCC 1 cut(s) 806
SspMI CTAG 3 cut(s) 1095, 1812, 1889
SstI GAGCTC 1 cut(s) 270
StyI CCWWGG 2 cut(s) 390, 1811
TaaI ACNGT 4 cut(s) 740, 1085, 1183, 1293
TaiI ACGT 5 cut(s) 44, 360, 459, 1946, 2055
TaqII GACCGA 3 cut(s) 415, 903, 1471
TasI AATT 8 cut(s) 461, 663, 676, 832, 1000, 1713, 1755, 1798
TatI WGTACW 1 cut(s) 1491
TauI GCSGC 2 cut(s) 807, 1205
TfiI GAWTC 9 cut(s) 4, 34, 224, 656, 1061, 1307, 1315, 1345, 2043
Tru1I TTAA 5 cut(s) 1158, 1242, 1605, 1662, 1670
Tru9I TTAA 5 cut(s) 1158, 1242, 1605, 1662, 1670
TscAI CASTG 2 cut(s) 1090, 1972
TseFI GTSAC 4 cut(s) 93, 353, 883, 1067
TseI GCWGC 5 cut(s) 714, 801, 1121, 1454, 1866
Tsp45I GTSAC 4 cut(s) 93, 353, 883, 1067
TspDTI ATGAA 8 cut(s) 44, 237, 359, 510, 1151, 1224, 1701, 2013
TspGWI ACGGA 4 cut(s) 48, 427, 529, 1001
TspMI CCCGGG 1 cut(s) 315
TspRI CASTG 2 cut(s) 1090, 1972
Van91I CCANNNNNTGG 1 cut(s) 333
VpaK11BI GGWCC 3 cut(s) 537, 1913, 1981
VspI ATTAAT 1 cut(s) 1605
XagI CCTNNNNNAGG 1 cut(s) 1815
XapI RAATTY 3 cut(s) 676, 1000, 1798
XceI RCATGY 1 cut(s) 1454
XcmI CCANNNNNNNNNTGG 1 cut(s) 1141
XhoI CTCGAG 2 cut(s) 599, 1947
XmaI CCCGGG 1 cut(s) 315
XmaJI CCTAGG 1 cut(s) 1811
XmiI GTMKAC 3 cut(s) 429, 501, 1358
XmnI GAANNNNTTC 3 cut(s) 213, 932, 1826
XspI CTAG 3 cut(s) 1095, 1812, 1889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.