pycom16g07910

At4g28440-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003500
Physical Location & Seq
Reverse (-)
5216666 .. 5218524
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 414 bp
ATGGCTGAAGCAAAACCAGGATTGAGGAAACCAGTATTCACCAAGGTTGGCCAGCTCCGCCCAAGTACTTCTGGGCACACTCTGACAGTAAAGGTTGTCAGTACTAAGATGGTGTTACAGAAGGGTCGTGCTGATGGTCCTCAAGGACGCCAAACGCGAATTGCTGAATGCTTGGTTGGTGATGAAACTGGTTTGATTATCTTCACTGCTAGAAACGATCAAGTGGACTTGATGCAAGAAGATTCTACCATAACCCTAAGGAATGCCAAGATTGACATGTGGAAAGGATCAATGAGACTTGCTGTGGACAAGTGGGGCCGTGTCGAAGTTGCTGAACCTGCCAGTTTTACGGTTAAGGAAGACAACAACCTCTCCCTTATTGAATATGAACTTGTCAATGTTGTTGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

15.2

Weight (kDa)

6.61

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_Ssb-like PF21473 19 - 110 3.2e-44 Single-stranded DNA binding protein Ssb-like, OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 346
AccII CGCG 1 cut(s) 157
AciI CCGC 1 cut(s) 58
AclWI GGATC 1 cut(s) 295
AcoI YGGCCR 1 cut(s) 49
AcuI CTGAAG 1 cut(s) 27
AcyI GRCGYC 1 cut(s) 148
AfaI GTAC 2 cut(s) 67, 103
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 2 cut(s) 383, 407
AjnI CCWGG 1 cut(s) 16
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 1 cut(s) 55
AluI AGCT 1 cut(s) 55
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 1 cut(s) 295
AoxI GGCC 2 cut(s) 49, 316
AspS9I GGNCC 2 cut(s) 137, 316
AsuHPI GGTGA 2 cut(s) 31, 191
AvaII GGWCC 1 cut(s) 137
AxyI CCTNAGG 1 cut(s) 257
BaeGI GKGCMC 1 cut(s) 78
BalI TGGCCA 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 366
BccI CCATC 2 cut(s) 103, 128
BceAI ACGGC 1 cut(s) 303
BciT130I CCWGG 1 cut(s) 18
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 1 cut(s) 210
BfuAI ACCTGC 1 cut(s) 346
BmcAI AGTACT 2 cut(s) 67, 103
Bme1390I CCNGG 1 cut(s) 18
Bme18I GGWCC 1 cut(s) 137
BmgT120I GGNCC 2 cut(s) 137, 316
BmiI GGNNCC 1 cut(s) 317
BmrFI CCNGG 1 cut(s) 18
BmsI GCATC 1 cut(s) 222
BpiI GAAGAC 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 148
BsaJI CCNNGG 1 cut(s) 42
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bse1I ACTGG 3 cut(s) 32, 193, 342
Bse21I CCTNAGG 1 cut(s) 257
BseBI CCWGG 1 cut(s) 18
BseDI CCNNGG 1 cut(s) 42
BseNI ACTGG 3 cut(s) 32, 193, 342
BseSI GKGCMC 1 cut(s) 78
Bsh1236I CGCG 1 cut(s) 157
BshFI GGCC 2 cut(s) 51, 318
BsmAI GTCTC 1 cut(s) 289
BsmI GAATGC 2 cut(s) 173, 268
BsnI GGCC 2 cut(s) 51, 318
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 2 cut(s) 217, 287
BspACI CCGC 1 cut(s) 58
BspANI GGCC 2 cut(s) 51, 318
BspFNI CGCG 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 317
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 1 cut(s) 295
BsrI ACTGG 3 cut(s) 32, 193, 342
BssECI CCNNGG 1 cut(s) 42
BssMI GATC 2 cut(s) 217, 287
BssNI GRCGYC 1 cut(s) 148
BssT1I CCWWGG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 18
Bst4CI ACNGT 2 cut(s) 88, 352
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 1 cut(s) 148
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 2 cut(s) 105, 257
BstFNI CGCG 1 cut(s) 157
BstKTI GATC 2 cut(s) 220, 290
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 2 cut(s) 217, 287
BstMWI GCNNNNNNNGC 2 cut(s) 57, 338
BstNI CCWGG 1 cut(s) 18
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 16
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 1 cut(s) 157
BstV2I GAAGAC 1 cut(s) 366
Bsu36I CCTNAGG 1 cut(s) 257
BsuRI GGCC 2 cut(s) 51, 318
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 1 cut(s) 204
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 137, 316
CseI GACGC 1 cut(s) 156
Csp6I GTAC 2 cut(s) 66, 102
CviAII CATG 1 cut(s) 277
CviJI RGCY 4 cut(s) 5, 51, 55, 318
CviKI_1 RGCY 4 cut(s) 5, 51, 55, 318
CviQI GTAC 2 cut(s) 66, 102
DdeI CTNAG 2 cut(s) 105, 257
DpnI GATC 2 cut(s) 219, 289
DpnII GATC 2 cut(s) 217, 287
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
EciI GGCGGA 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 137
Eco57I CTGAAG 1 cut(s) 27
Eco81I CCTNAGG 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 16
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 280
FaiI YATR 3 cut(s) 251, 278, 387
FatI CATG 1 cut(s) 276
FspBI CTAG 1 cut(s) 210
HaeIII GGCC 2 cut(s) 51, 318
HgaI GACGC 1 cut(s) 156
Hin1I GRCGYC 1 cut(s) 148
Hin1II CATG 1 cut(s) 280
HinfI GANTC 1 cut(s) 242
HphI GGTGA 2 cut(s) 31, 191
Hpy166II GTNNAC 2 cut(s) 226, 307
Hpy188I TCNGA 1 cut(s) 84
Hpy8I GTNNAC 2 cut(s) 226, 307
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 2 cut(s) 88, 352
HpyCH4V TGCA 1 cut(s) 235
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 338
HpyF3I CTNAG 2 cut(s) 105, 257
Hsp92I GRCGYC 1 cut(s) 148
Hsp92II CATG 1 cut(s) 280
Kzo9I GATC 2 cut(s) 217, 287
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 8 cut(s) 3, 30, 45, 57, 65, 174, 351, 355
LweI GCATC 1 cut(s) 222
MaeI CTAG 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 114
MalI GATC 2 cut(s) 219, 289
MboI GATC 2 cut(s) 217, 287
MboII GAAGA 3 cut(s) 193, 251, 371
MhlI GDGCHC 1 cut(s) 78
MlsI TGGCCA 1 cut(s) 51
MluCI AATT 1 cut(s) 159
MluNI TGGCCA 1 cut(s) 51
MnlI CCTC 3 cut(s) 18, 150, 380
Mox20I TGGCCA 1 cut(s) 51
MscI TGGCCA 1 cut(s) 51
MseI TTAA 1 cut(s) 354
Msp20I TGGCCA 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 18
Mva1269I GAATGC 2 cut(s) 173, 268
MvaI CCWGG 1 cut(s) 18
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 2 cut(s) 57, 338
NdeII GATC 2 cut(s) 217, 287
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 1 cut(s) 317
NspI RCATGY 1 cut(s) 280
PciI ACATGT 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 154
PctI GAATGC 2 cut(s) 173, 268
PfeI GAWTC 1 cut(s) 242
PscI ACATGT 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 317
PspPI GGNCC 2 cut(s) 137, 316
RsaI GTAC 2 cut(s) 67, 103
RsaNI GTAC 2 cut(s) 66, 102
SaqAI TTAA 1 cut(s) 354
Sau3AI GATC 2 cut(s) 217, 287
Sau96I GGNCC 2 cut(s) 137, 316
ScaI AGTACT 2 cut(s) 67, 103
ScrFI CCNGG 1 cut(s) 18
SduI GDGCHC 1 cut(s) 78
SetI ASST 5 cut(s) 48, 57, 96, 340, 372
SfaNI GCATC 1 cut(s) 222
SinI GGWCC 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
Sse9I AATT 1 cut(s) 159
SsiI CCGC 1 cut(s) 58
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 16
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 88, 352
TaqI TCGA 1 cut(s) 324
TasI AATT 1 cut(s) 159
TatI WGTACW 2 cut(s) 65, 101
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 1 cut(s) 211
TspDTI ATGAA 2 cut(s) 198, 402
TspRI CASTG 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 137
XceI RCATGY 1 cut(s) 280
XspI CTAG 1 cut(s) 210
ZrmI AGTACT 2 cut(s) 67, 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.