Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0002043

Physical Location & Seq

Genomic Coordinates Forward (+)

5979747 .. 5983263

Gene Structure 3517 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2433 bp

ATGGCAGCTCCTCAGAATTATAATAACCCCCCTGTACCGGCTGCCCAGAATTACAATCCTACCCCTATGCCAGCTGCTCAGAATTACAATCCTGCCCCTATTCCAGCTGCTCAGAATTACAATCCTTACGATCCGGCCCGTGTGCCGGCTGCTCAGAGTTATAATGCACCCCAGGCACCAGGTGCTCAGAGTTATAATGCACCCCAGGCACCAGGTGCTCAGTATTACAATAAGCCCACCGCCGGAGGACGCAGAGGAGGAGACTACTATAACCGCCCTCTATCCAAAGATGGGCGTCGCTTTTCTTCAACAGCTTCTGATGACAGTATGCTGACCAACCAAATTCTGGCTAGTGACAAGTCTCATGCCCGTCCCTATGATGTCACTCTCCTTTCTTTGAAACACATTCTTCAAACCGTCGACGTGATTCTGTCCCGCGTCACCCAACCTGACATCCACAGCACCGTCACGGTAATGCCGGGTGCACACGCCGATGCTTTGGAGCATGAGAAGGCTTCCCATGCTATCCTCAGTAGTCTCCCTGACAACTACGATGTTCCAACCAGCCTGTTTAATGCAATTTCCTGCGAGATATTCTGCAAGTGGCTGTCTGGGGAAGATGCCAACAAGACAACCATGGACATATTAGACACAGTGCAACACTACGACTGGGATGAAAAGGTTGTGCTTGCCTTGGGAGCTTTTGCTGTGAAAGATGGTGAATACTGGCTTGTGGCTCACCTTTACACCACCAACCCTCTCGCCAAAGCGGTTGGGCAGCTTAAGCAAGTGCAAGAGATACTGGAACATGCCGGGACTCGTTTGAAGCCCAAGTTTGACGCCTACAACAATCTGGTTAGGGCCGTGCTCAATGTGACCAAGTGCATTGTTCAGCTACATGACCTCCACCGCAACCCCGTCATGACCACTGAAGCAGAGTCGGCGGCTACCACTGCTCTTATCCCCACAGCTGTTTACTGGACGATTCGGAGTATTGTAGTTGCTGCAACACAGCATTTGGGGATCACTGGCATGGGCCCCGAGCATATGACAGAGGCATGGGAACTGTCTTCCTTGGCTCATAAGCTCGAAAACATACACAGCCACCTCAAGGAGAATCTCGACAAATTACTCGACATCATCAAGAGGAAGAAAGAAGAGCAAGAATTAAGTGAAATTTCTTACATCTTGGAATCACCACACATTGACAACAGCAAGCCTTTGAGGGTCCTGTTTTACAAGGATGATCAGCCTGCACTCTATGATTGCTTCAACAAGAAGAGGGTTGACATTGATGTGCTGAAGAGGAAGGTTGTGATACTGTTCCTTTCGGACACAGACGTTGTCCACGAAAACGAGTACATGATTGTTCAGCAAATGTACTTGGAAAAACGGCAGAACCCAACCAGGACGGAGAGCCAGTATGAGGTTGTGTGGGTTCCGATTGTGGACATGTGGACCGATGCCAAGTACCAGCAGTTCGAGGATCTTAGAAGGAACATGGAATGGTACACATTGTTCCACCCTTCGGTTGTCCCTAAGACTGTCATCAGGTACATCAGGAAGCAGGATAAATGGAACTATGGCAAGAAGCCTCTGCTTGTGGTGATGGACCCTCAAGGAAAAATAGTGCACACCAATGCTGTTCACATGATGTGTGTTTGGGGAAGCATAGCCTTCCCTTTCACTAGCAACAGAGAGAAATTACTCTGGGAGGAAGAGAAGGATTGGAGGATCGAGCTTTTGGCAGATTCTATCGATCAGAACCTAATTACCTGGATCACGGAAGGGAAATACATTTGCTTGTATGGCGGGGAAGATATAGATTGGATCAGAAATTTCACAAGGTCAGCCAAAAATGTGGCTCGGGAAGCTGGGATCCAGTTGGAATTGCTTTATGTGGGGAAAAGAAACCCTAAGGAGAAAGTGGTGAGGAACATCATGAACGTCATCCAAGCTGAGAAGCTAAGCCACACTCTTGAGTGGACCCTCATCTGGTTCTTCTGGATGCGTCTCGAGAGCATGTGGCAATCCAAGGGACACCAGATGCAGTCTGAGGTTAGGTCTGGTCGAGTTAGGACCGACAACAAGAAGAATGATCCAGTGATGCAGGGGATCATATCGATGTTGAGCTTCGGTTCAAGTGACCGTGGATGGGCTGTGATTGGCGCCGGTTCATCGGAGATGGCTAAGGCTAATGGAGAGCACATGCATAGAAGTTTAAGCGAGTATAACTTGTGGAATAGAAGGGTGAACGAGATAGGGTTTGTGCCTGCACTGAATGAGTACTTGACTGGGGTTTATAAAGAAGCTCCACATCACTGCACCAGTCTCATATTGCCTGCCACCGGACTCATGCCTGAGACGGTGGCCTGCGCTGAATGCGGCCGCCTCATGGAGCGGTTCACCATGTTCCGCTGCTGCACTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0032502 GO:0042802 GO:0042803 GO:0043621 GO:0046983 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SEO_C SEO_N

Protein Analysis

811

Amino Acids

92.28

Weight (kDa)

6.47

Isoelectric Point (pI)

45.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SEO_N	PF14576	106 - 401	3.7e-74	Sieve element occlusion N-terminus
SEO_C	PF14577	566 - 809	6.3e-88	Sieve element occlusion C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002043)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25290 FvH4_4g25290 FvH4_4g25290 FvH4_4g25310 FvH4_4g25320
malus_domestica	MD13G1105000.v1.1 MD13G1105100.v1.1 MD16G1104800.v1.1 MD16G1105000.v1.1
prunus_persica	Prupe.1G241400_v2.0.a1 Prupe.1G241500_v2.0.a1
pyrus_communis	pycom13g09110 pycom13g09120 pycom16g08950 pycom16g08960
rosa_chinensis	RchiOBHm_Chr4g0432561 RchiOBHm_Chr4g0432571 RchiOBHm_Chr4g0432611
rosa_laevigata	RLG00000006831 RLG00000006832 RLG00000006833
rosa_multiflora	Rmu_sc0003886.1_g000001 Rmu_sc0004612.1_g000014 Rmu_sc0027699.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373990 Rroxscaffold_5G00374000 Rroxscaffold_5G00374030
rosa_rugosa	Rorug04G0260100 Rorug04G0260100 Rorug04G0260200
rosa_samantha	Rh4AG315100 Rh4AG315200 Rh4AG315300 Rh4BG322800 Rh4BG322900 Rh4BG323000 Rh4CG337900 Rh4CG338000 Rh4CG338100 Rh4DG318600 Rh4DG318700 Rh4DG318800
rosa_wichuraiana	Rw4G027380 Rw4G027390 Rw4G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	21, 162, 195, 2304
AccB1I	GGYRCC	3 cut(s)	175, 208, 2168
AccB7I	CCANNNNNTGG	1 cut(s)	346
AccBSI	CCGCTC	1 cut(s)	2401
AccI	GTMKAC	1 cut(s)	420
AccII	CGCG	1 cut(s)	438
AcoI	YGGCCR	1 cut(s)	2386
AcsI	RAATTY	3 cut(s)	342, 1176, 1837
AcuI	CTGAAG	2 cut(s)	951, 1322
AcyI	GRCGYC	3 cut(s)	295, 840, 2169
AdeI	CACNNNGTG	2 cut(s)	182, 215
AfaI	GTAC	7 cut(s)	36, 1361, 1382, 1472, 1511, 1556, 2288
AflII	CTTAAG	1 cut(s)	782
AflIII	ACRYGT	1 cut(s)	1452
AgsI	TTSAA	6 cut(s)	309, 400, 413, 826, 1273, 2142
AjiI	CACGTC	1 cut(s)	424
AjnI	CCWGG	6 cut(s)	171, 178, 204, 211, 1406, 1775
Alw21I	GWGCWC	6 cut(s)	187, 220, 487, 870, 1635, 2208
Alw26I	GTCTC	6 cut(s)	255, 366, 542, 2018, 2336, 2357
Alw44I	GTGCAC	2 cut(s)	483, 1631
AlwNI	CAGNNNCTG	1 cut(s)	317
Ama87I	CYCGRG	3 cut(s)	1040, 1866, 2015
AoxI	GGCC	5 cut(s)	135, 861, 1036, 2370, 2386
ApaI	GGGCCC	1 cut(s)	1040
ApaLI	GTGCAC	2 cut(s)	483, 1631
ApeKI	GCWGC	9 cut(s)	5, 41, 74, 107, 149, 778, 1004, 2418, 2421
ApoI	RAATTY	3 cut(s)	342, 1176, 1837
AspLEI	GCGC	2 cut(s)	2171, 2378
AspS9I	GGNCC	9 cut(s)	136, 861, 1036, 1037, 1228, 1458, 1612, 1986, 2079
AsuC2I	CCSGG	2 cut(s)	480, 814
AsuHPI	GGTGA	8 cut(s)	433, 731, 731, 1188, 1618, 1942, 2263, 2398
AvaI	CYCGRG	3 cut(s)	1040, 1866, 2015
AvaII	GGWCC	5 cut(s)	1228, 1458, 1612, 1986, 2079
AxyI	CCTNAGG	1 cut(s)	1917
BaeGI	GKGCMC	3 cut(s)	487, 1040, 1635
BamHI	GGATCC	1 cut(s)	1878
BanI	GGYRCC	3 cut(s)	175, 208, 2168
BanII	GRGCYC	1 cut(s)	1040
BbsI	GAAGAC	1 cut(s)	1062
Bbv12I	GWGCWC	6 cut(s)	187, 220, 487, 870, 1635, 2208
BbvI	GCAGC	9 cut(s)	17, 28, 61, 94, 136, 790, 991, 2405, 2408
BccI	CCATC	5 cut(s)	284, 710, 1603, 2148, 2179
BceAI	ACGGC	2 cut(s)	848, 1409
BciT130I	CCWGG	6 cut(s)	173, 180, 206, 213, 1408, 1777
BclI	TGATCA	1 cut(s)	1246
BcnI	CCSGG	2 cut(s)	480, 814
BcoDI	GTCTC	6 cut(s)	255, 366, 542, 2018, 2336, 2357
BfaI	CTAG	2 cut(s)	351, 1689
BfoI	RGCGCY	1 cut(s)	2172
BfrI	CTTAAG	1 cut(s)	782
BlpI	GCTNAGC	1 cut(s)	1967
BmcAI	AGTACT	1 cut(s)	2288
Bme1390I	CCNGG	8 cut(s)	173, 180, 206, 213, 480, 814, 1408, 1777
Bme18I	GGWCC	5 cut(s)	1228, 1458, 1612, 1986, 2079
BmeT110I	CYCGRG	3 cut(s)	1040, 1866, 2015
BmgBI	CACGTC	1 cut(s)	424
BmgT120I	GGNCC	9 cut(s)	136, 861, 1036, 1037, 1228, 1458, 1612, 1986, 2079
BmrFI	CCNGG	8 cut(s)	173, 180, 206, 213, 480, 814, 1408, 1777
BmrI	ACTGGG	2 cut(s)	679, 2304
BmsI	GCATC	6 cut(s)	484, 610, 1453, 1998, 2037, 2097
BmuI	ACTGGG	2 cut(s)	679, 2304
BpiI	GAAGAC	1 cut(s)	1062
Bpu10I	CCTNAGC	1 cut(s)	2190
Bpu1102I	GCTNAGC	1 cut(s)	1967
BpuEI	CTTGAG	3 cut(s)	1094, 1602, 2000
BpuMI	CCSGG	2 cut(s)	480, 814
Bsa29I	ATCGAT	2 cut(s)	1758, 2123
BsaHI	GRCGYC	3 cut(s)	295, 840, 2169
BsaJI	CCNNGG	7 cut(s)	171, 204, 636, 693, 1074, 2034, 2149
BsaWI	WCCGGW	1 cut(s)	2348
BsaXI	ACNNNNNCTCC	4 cut(s)	888, 918, 1406, 1436
Bse118I	RCCGGY	3 cut(s)	37, 145, 2171
Bse21I	CCTNAGG	1 cut(s)	1917
BseBI	CCWGG	6 cut(s)	173, 180, 206, 213, 1408, 1777
BseCI	ATCGAT	2 cut(s)	1758, 2123
BseDI	CCNNGG	7 cut(s)	171, 204, 636, 693, 1074, 2034, 2149
BseGI	GGATG	6 cut(s)	453, 679, 1249, 1950, 2013, 2159
BseRI	GAGGAG	2 cut(s)	270, 273
BseSI	GKGCMC	3 cut(s)	487, 1040, 1635
BseX3I	CGGCCG	1 cut(s)	2386
BseXI	GCAGC	9 cut(s)	17, 28, 61, 94, 136, 790, 991, 2405, 2408
BseYI	CCCAGC	1 cut(s)	1874
BsgI	GTGCAG	4 cut(s)	1239, 2259, 2308, 2407
Bsh1236I	CGCG	1 cut(s)	438
Bsh1285I	CGRYCG	1 cut(s)	2389
BshFI	GGCC	5 cut(s)	137, 863, 1038, 2372, 2388
BshNI	GGYRCC	3 cut(s)	175, 208, 2168
BshVI	ATCGAT	2 cut(s)	1758, 2123
BsiEI	CGRYCG	1 cut(s)	2389
BsiHKAI	GWGCWC	6 cut(s)	187, 220, 487, 870, 1635, 2208
BsiHKCI	CYCGRG	3 cut(s)	1040, 1866, 2015
BsiSI	CCGG	8 cut(s)	38, 134, 146, 243, 479, 813, 2172, 2349
BslFI	GGGAC	5 cut(s)	357, 418, 829, 1520, 2052
BsmAI	GTCTC	6 cut(s)	255, 366, 542, 2018, 2336, 2357
BsmBI	CGTCTC	2 cut(s)	2018, 2357
BsmFI	GGGAC	5 cut(s)	357, 418, 829, 1520, 2052
BsmI	GAATGC	1 cut(s)	2387
BsnI	GGCC	5 cut(s)	137, 863, 1038, 2372, 2388
BsoBI	CYCGRG	3 cut(s)	1040, 1866, 2015
Bsp120I	GGGCCC	1 cut(s)	1036
Bsp1286I	GDGCHC	7 cut(s)	187, 220, 487, 870, 1040, 1635, 2208
Bsp1720I	GCTNAGC	1 cut(s)	1967
Bsp19I	CCATGG	1 cut(s)	636
BspANI	GGCC	5 cut(s)	137, 863, 1038, 2372, 2388
BspDI	ATCGAT	2 cut(s)	1758, 2123
BspFNI	CGCG	1 cut(s)	438
BspHI	TCATGA	2 cut(s)	921, 1941
BspQI	GCTCTTC	1 cut(s)	1152
BspT107I	GGYRCC	3 cut(s)	175, 208, 2168
BspTI	CTTAAG	1 cut(s)	782
BsrBI	CCGCTC	1 cut(s)	2401
BsrFI	RCCGGY	3 cut(s)	37, 145, 2171
BssAI	RCCGGY	3 cut(s)	37, 145, 2171
BssECI	CCNNGG	7 cut(s)	171, 204, 636, 693, 1074, 2034, 2149
BssNI	GRCGYC	3 cut(s)	295, 840, 2169
BssT1I	CCWWGG	4 cut(s)	636, 693, 1074, 2034
Bst2UI	CCWGG	6 cut(s)	173, 180, 206, 213, 1408, 1777
Bst6I	CTCTTC	4 cut(s)	1152, 1274, 1298, 1713
BstACI	GRCGYC	3 cut(s)	295, 840, 2169
BstAFI	CTTAAG	1 cut(s)	782
BstAPI	GCANNNNNTGC	2 cut(s)	182, 215
BstC8I	GCNNGC	8 cut(s)	72, 147, 690, 1217, 1254, 2274, 2343, 2374
BstDSI	CCRYGG	2 cut(s)	636, 2149
BstF5I	GGATG	6 cut(s)	453, 679, 1249, 1950, 2013, 2159
BstFNI	CGCG	1 cut(s)	438
BstH2I	RGCGCY	1 cut(s)	2172
BstHHI	GCGC	2 cut(s)	2171, 2378
BstMAI	GTCTC	6 cut(s)	255, 366, 542, 2018, 2336, 2357
BstMCI	CGRYCG	1 cut(s)	2389
BstNI	CCWGG	6 cut(s)	173, 180, 206, 213, 1408, 1777
BstNSI	RCATGY	4 cut(s)	812, 1456, 2026, 2212
BstSCI	CCNGG	8 cut(s)	171, 178, 204, 211, 478, 812, 1406, 1775
BstSLI	GKGCMC	3 cut(s)	487, 1040, 1635
BstUI	CGCG	1 cut(s)	438
BstV1I	GCAGC	9 cut(s)	17, 28, 61, 94, 136, 790, 991, 2405, 2408
BstV2I	GAAGAC	1 cut(s)	1062
BstX2I	RGATCY	2 cut(s)	1486, 1878
BstXI	CCANNNNNNTGG	1 cut(s)	1861
BstYI	RGATCY	2 cut(s)	1486, 1878
BstZI	CGGCCG	1 cut(s)	2386
Bsu15I	ATCGAT	2 cut(s)	1758, 2123
Bsu36I	CCTNAGG	1 cut(s)	1917
BsuRI	GGCC	5 cut(s)	137, 863, 1038, 2372, 2388
BsuTUI	ATCGAT	2 cut(s)	1758, 2123
BtgI	CCRYGG	2 cut(s)	636, 2149
BtrI	CACGTC	1 cut(s)	424
BtsCI	GGATG	6 cut(s)	453, 679, 1249, 1950, 2013, 2159
BtsI	GCAGTG	2 cut(s)	951, 2320
BtsIMutI	CAGTG	8 cut(s)	660, 927, 951, 1026, 2109, 2276, 2320, 2424
Cac8I	GCNNGC	8 cut(s)	72, 147, 690, 1217, 1254, 2274, 2343, 2374
CaiI	CAGNNNCTG	1 cut(s)	317
CciI	TCATGA	2 cut(s)	921, 1941
CciNI	GCGGCCGC	1 cut(s)	2386
CfoI	GCGC	2 cut(s)	2171, 2378
Cfr10I	RCCGGY	3 cut(s)	37, 145, 2171
Cfr13I	GGNCC	9 cut(s)	136, 861, 1036, 1037, 1228, 1458, 1612, 1986, 2079
ClaI	ATCGAT	2 cut(s)	1758, 2123
CseI	GACGC	5 cut(s)	258, 284, 427, 848, 2000
CsiI	ACCWGGT	2 cut(s)	178, 211
Csp6I	GTAC	7 cut(s)	35, 1360, 1381, 1471, 1510, 1555, 2287
CviQI	GTAC	7 cut(s)	35, 1360, 1381, 1471, 1510, 1555, 2287
DinI	GGCGCC	1 cut(s)	2170
DraIII	CACNNNGTG	2 cut(s)	182, 215
EaeI	YGGCCR	1 cut(s)	2386
EagI	CGGCCG	1 cut(s)	2386
Eam1104I	CTCTTC	4 cut(s)	1152, 1274, 1298, 1713
EarI	CTCTTC	4 cut(s)	1152, 1274, 1298, 1713
EclXI	CGGCCG	1 cut(s)	2386
Eco130I	CCWWGG	4 cut(s)	636, 693, 1074, 2034
Eco24I	GRGCYC	1 cut(s)	1040
Eco47I	GGWCC	5 cut(s)	1228, 1458, 1612, 1986, 2079
Eco52I	CGGCCG	1 cut(s)	2386
Eco57I	CTGAAG	2 cut(s)	951, 1322
Eco81I	CCTNAGG	1 cut(s)	1917
Eco88I	CYCGRG	3 cut(s)	1040, 1866, 2015
EcoO109I	RGGNCCY	2 cut(s)	1037, 1228
EcoRII	CCWGG	6 cut(s)	171, 178, 204, 211, 1406, 1775
EcoT14I	CCWWGG	4 cut(s)	636, 693, 1074, 2034
EcoT22I	ATGCAT	1 cut(s)	2214
EcoT38I	GRGCYC	1 cut(s)	1040
EgeI	GGCGCC	1 cut(s)	2170
EheI	GGCGCC	1 cut(s)	2170
ErhI	CCWWGG	4 cut(s)	636, 693, 1074, 2034
Esp3I	CGTCTC	2 cut(s)	2018, 2357
FaqI	GGGAC	5 cut(s)	357, 418, 829, 1520, 2052
FauI	CCCGC	2 cut(s)	443, 1805
FauNDI	CATATG	1 cut(s)	1047
FbaI	TGATCA	1 cut(s)	1246
FblI	GTMKAC	1 cut(s)	420
FokI	GGATG	6 cut(s)	440, 686, 1256, 1937, 2020, 2166
FriOI	GRGCYC	1 cut(s)	1040
FspBI	CTAG	2 cut(s)	351, 1689
GlaI	GCGC	2 cut(s)	2170, 2377
GsaI	CCCAGC	1 cut(s)	1878
HaeII	RGCGCY	1 cut(s)	2172
HaeIII	GGCC	5 cut(s)	137, 863, 1038, 2372, 2388
HapII	CCGG	8 cut(s)	38, 134, 146, 243, 479, 813, 2172, 2349
HgaI	GACGC	5 cut(s)	258, 284, 427, 848, 2000
HhaI	GCGC	2 cut(s)	2171, 2378
Hin1I	GRCGYC	3 cut(s)	295, 840, 2169
Hin6I	GCGC	2 cut(s)	2169, 2376
HinP1I	GCGC	2 cut(s)	2169, 2376
HincII	GTYRAC	2 cut(s)	421, 1288
HindII	GTYRAC	2 cut(s)	421, 1288
HinfI	GANTC	8 cut(s)	427, 817, 938, 985, 1117, 1193, 1751, 2352
HpaII	CCGG	8 cut(s)	38, 134, 146, 243, 479, 813, 2172, 2349
HphI	GGTGA	8 cut(s)	433, 731, 731, 1188, 1618, 1942, 2263, 2398
Hpy99I	CGWCG	3 cut(s)	300, 422, 425
HpyAV	CCTTC	8 cut(s)	505, 1303, 1488, 1536, 1687, 1717, 1781, 2241
HpyCH4IV	ACGT	3 cut(s)	423, 1341, 1947
HpySE526I	ACGT	3 cut(s)	423, 1341, 1947
Hsp92I	GRCGYC	3 cut(s)	295, 840, 2169
HspAI	GCGC	2 cut(s)	2169, 2376
KasI	GGCGCC	1 cut(s)	2168
KroI	GCCGGC	1 cut(s)	145
KroNI	GCCGGC	1 cut(s)	147
Ksp22I	TGATCA	1 cut(s)	1246
LguI	GCTCTTC	1 cut(s)	1152
LmnI	GCTCC	5 cut(s)	13, 502, 698, 2317, 2398
Lsp1109I	GCAGC	9 cut(s)	17, 28, 61, 94, 136, 790, 991, 2405, 2408
LweI	GCATC	6 cut(s)	484, 610, 1453, 1998, 2037, 2097
MabI	ACCWGGT	2 cut(s)	178, 211
MaeI	CTAG	2 cut(s)	351, 1689
MaeII	ACGT	3 cut(s)	423, 1341, 1947
MaeIII	GTNAC	6 cut(s)	353, 382, 439, 466, 874, 2144
MbiI	CCGCTC	1 cut(s)	2401
MflI	RGATCY	2 cut(s)	1486, 1878
MhlI	GDGCHC	7 cut(s)	187, 220, 487, 870, 1040, 1635, 2208
Mly113I	GGCGCC	1 cut(s)	2169
MlyI	GAGTC	3 cut(s)	811, 947, 2346
MmeI	TCCRAC	2 cut(s)	584, 1866
Mph1103I	ATGCAT	1 cut(s)	2214
MroNI	GCCGGC	1 cut(s)	145
MseI	TTAA	4 cut(s)	573, 783, 1169, 2222
MslI	CAYNNNNRTG	6 cut(s)	473, 492, 1031, 1295, 1638, 2123
MspA1I	CMGCKG	4 cut(s)	74, 107, 971, 2418
MspCI	CTTAAG	1 cut(s)	782
MspI	CCGG	8 cut(s)	38, 134, 146, 243, 479, 813, 2172, 2349
MspR9I	CCNGG	8 cut(s)	173, 180, 206, 213, 480, 814, 1408, 1777
Mva1269I	GAATGC	1 cut(s)	2387
MvaI	CCWGG	6 cut(s)	173, 180, 206, 213, 1408, 1777
MvnI	CGCG	1 cut(s)	438
NaeI	GCCGGC	1 cut(s)	147
NarI	GGCGCC	1 cut(s)	2169
NciI	CCSGG	2 cut(s)	480, 814
NcoI	CCATGG	1 cut(s)	636
NdeI	CATATG	1 cut(s)	1047
NgoMIV	GCCGGC	1 cut(s)	145
NmuCI	GTSAC	6 cut(s)	353, 382, 439, 466, 874, 2144
NotI	GCGGCCGC	1 cut(s)	2386
NsiI	ATGCAT	1 cut(s)	2214
NspI	RCATGY	4 cut(s)	812, 1456, 2026, 2212
PaeR7I	CTCGAG	1 cut(s)	2015
PagI	TCATGA	2 cut(s)	921, 1941
PciI	ACATGT	1 cut(s)	1452
PciSI	GCTCTTC	1 cut(s)	1152
PcsI	WCGNNNNNNNCGW	1 cut(s)	1347
PctI	GAATGC	1 cut(s)	2387
PdiI	GCCGGC	1 cut(s)	147
PfeI	GAWTC	5 cut(s)	427, 985, 1117, 1193, 1751
PflFI	GACNNNGTC	1 cut(s)	1343
PflMI	CCANNNNNTGG	1 cut(s)	346
PleI	GAGTC	3 cut(s)	811, 946, 2346
PluTI	GGCGCC	1 cut(s)	2172
PpsI	GAGTC	3 cut(s)	811, 946, 2346
PpuMI	RGGWCCY	1 cut(s)	1228
PscI	ACATGT	1 cut(s)	1452
PsiI	TTATAA	4 cut(s)	21, 162, 195, 2304
Psp5II	RGGWCCY	1 cut(s)	1228
Psp6I	CCWGG	6 cut(s)	171, 178, 204, 211, 1406, 1775
PspFI	CCCAGC	1 cut(s)	1874
PspGI	CCWGG	6 cut(s)	171, 178, 204, 211, 1406, 1775
PspOMI	GGGCCC	1 cut(s)	1036
PspPI	GGNCC	9 cut(s)	136, 861, 1036, 1037, 1228, 1458, 1612, 1986, 2079
PspPPI	RGGWCCY	1 cut(s)	1228
PsrI	GAACNNNNNNTAC	4 cut(s)	1463, 1495, 1502, 1534
PstNI	CAGNNNCTG	1 cut(s)	317
PsuI	RGATCY	2 cut(s)	1486, 1878
PsyI	GACNNNGTC	1 cut(s)	1343
PvuII	CAGCTG	3 cut(s)	74, 107, 971
RsaI	GTAC	7 cut(s)	36, 1361, 1382, 1472, 1511, 1556, 2288
RsaNI	GTAC	7 cut(s)	35, 1360, 1381, 1471, 1510, 1555, 2287
RseI	CAYNNNNRTG	6 cut(s)	473, 492, 1031, 1295, 1638, 2123
SalI	GTCGAC	1 cut(s)	419
SapI	GCTCTTC	1 cut(s)	1152
SaqAI	TTAA	4 cut(s)	573, 783, 1169, 2222
Sau96I	GGNCC	9 cut(s)	136, 861, 1036, 1037, 1228, 1458, 1612, 1986, 2079
ScaI	AGTACT	1 cut(s)	2288
SchI	GAGTC	3 cut(s)	811, 947, 2346
ScrFI	CCNGG	8 cut(s)	173, 180, 206, 213, 480, 814, 1408, 1777
SduI	GDGCHC	7 cut(s)	187, 220, 487, 870, 1040, 1635, 2208
SexAI	ACCWGGT	2 cut(s)	178, 211
SfaNI	GCATC	6 cut(s)	484, 610, 1453, 1998, 2037, 2097
SfoI	GGCGCC	1 cut(s)	2170
Sfr274I	CTCGAG	1 cut(s)	2015
SgrDI	CGTCGACG	1 cut(s)	419
SinI	GGWCC	5 cut(s)	1228, 1458, 1612, 1986, 2079
SlaI	CTCGAG	1 cut(s)	2015
SmiMI	CAYNNNNRTG	6 cut(s)	473, 492, 1031, 1295, 1638, 2123
SmlI	CTYRAG	5 cut(s)	782, 1109, 1617, 1979, 2015
SmoI	CTYRAG	5 cut(s)	782, 1109, 1617, 1979, 2015
SspDI	GGCGCC	1 cut(s)	2168
SspMI	CTAG	2 cut(s)	351, 1689
StyD4I	CCNGG	8 cut(s)	171, 178, 204, 211, 478, 812, 1406, 1775
StyI	CCWWGG	4 cut(s)	636, 693, 1074, 2034
TaiI	ACGT	3 cut(s)	426, 1344, 1950
TaqII	GACCGA	2 cut(s)	1475, 2096
TatI	WGTACW	3 cut(s)	1359, 1380, 2286
TauI	GCSGC	3 cut(s)	947, 2388, 2391
TfiI	GAWTC	5 cut(s)	427, 985, 1117, 1193, 1751
Tru1I	TTAA	4 cut(s)	573, 783, 1169, 2222
Tru9I	TTAA	4 cut(s)	573, 783, 1169, 2222
TscAI	CASTG	8 cut(s)	660, 934, 958, 1033, 2109, 2283, 2327, 2431
TseFI	GTSAC	6 cut(s)	353, 382, 439, 466, 874, 2144
TseI	GCWGC	9 cut(s)	5, 41, 74, 107, 149, 778, 1004, 2418, 2421
Tsp45I	GTSAC	6 cut(s)	353, 382, 439, 466, 874, 2144
TspDTI	ATGAA	3 cut(s)	690, 1958, 2166
TspGWI	ACGGA	2 cut(s)	1427, 1799
TspRI	CASTG	8 cut(s)	660, 934, 958, 1033, 2109, 2283, 2327, 2431
Tth111I	GACNNNGTC	1 cut(s)	1343
Van91I	CCANNNNNTGG	1 cut(s)	346
Vha464I	CTTAAG	1 cut(s)	782
VneI	GTGCAC	2 cut(s)	483, 1631
VpaK11BI	GGWCC	5 cut(s)	1228, 1458, 1612, 1986, 2079
XapI	RAATTY	3 cut(s)	342, 1176, 1837
XceI	RCATGY	4 cut(s)	812, 1456, 2026, 2212
XcmI	CCANNNNNNNNNTGG	1 cut(s)	343
XhoI	CTCGAG	1 cut(s)	2015
XmiI	GTMKAC	1 cut(s)	420
XspI	CTAG	2 cut(s)	351, 1689
ZrmI	AGTACT	1 cut(s)	2288
Zsp2I	ATGCAT	1 cut(s)	2214

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.