Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0002043

Physical Location & Seq

Genomic Coordinates Forward (+)

5984717 .. 5987608

Gene Structure 2892 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2139 bp

ATGGCCCTGGTACCTCACAATAAGGCTGTCAACCCAACAAGCAGAGGAGCCGGCCATAATCGCCCTCCTCTGCTTAAACAAGGGCGTGACGGACATCGTCAATTTTCGAAAACAGCAGATGACAGTGCGTTGGTCAACCAAATTATGCTTACTGATAAGTCTCATGACCATCCCTACGAAGTGCCTCCTCTTGCAGTCACGCAAATTCTTCAAACCATTGAGGCCATTCTTTCGCGCGTCACCAAACCTGACATCCATGATGGCATTCTAGTCCCGGGAGCATTAGCACCGGCAGCTACACGCGCGGATGCTTTGGAGCATGAGAAGACCCTCCATGCTAACCTGAGTACCCTCCACGACAGCGTTGATGTTCCACACAGCGTGTTCAATGCAATTCACTCCGAGTTAATGTGCACATTTGTGCAAGGGGAAGATGCAAACAAGACAACAATGGACATACTAGACATTGTGCAACACTACGATTGGGATGAAAAGGTGGTGCTTGCCTTGGGAGCATTTGCGATCAAAGATGGCGAGTTTTGGCTCGTGGCTCAGCAATACACCACCAACCCACTCGCCAGAGCGGTTGGACAGCTTAAGCAACTGCAAGAGATCTTGGAACGTGCGGGGACTAGTTTGAAAACGAAGTTCGATGCATACAACAGTCTGGTTGGGGCCATTTTCAAAGTGACCAAGACCATTGTTCACTTACAGGAGATTCACAACGATCCCCAATTGAGCCCCGAAATCAAAGCTACAGCTGCCACTGCTCAGACCCCCACAGCAGTTTACTGGACAATTCGGGCTATTGTAGTTGCTGCATCACAACTTCTGGGAATCACAGGCTCAGAGCCAGAGTATGTGACAGAGGCGTGGGAACTATCATCGCTGGCCCATAAGCTCAAAAACATATTCGATCACCTTGAAGATATCCTCGACAGATTACATCAACTTATCTGGAAGAACAAAGAAGAGGAAGCTTTCAACACAATCGCGCGCATCTTGGAAACACCACACATGGATAACTCGAAGCCTCTGAGGGTTTTGTTTTACAAGGATGATCAGCCTGCACTATATGACGGCTACAACAAGAAGAGGGTTGACATTGATGTGCTGAAGAGGAAAGTAGTGATTCTGTTCATCACAGACCTAGACGTTGTCCACGAAAACGAGTACATTATTGTCCTACAAATGAACTCGGAAAAGCTACACAACCCTACAAGGCCGGAGAGCCAATACGAGGTTGTGTGGGTTCCAATTACGGATGCATGGACTGAAGCCAAGTACAAGCAATTCGAGAGTCTAAAAGATAGCATGCAATGGCACACCGTGTTTCACCCTTCTGTAGTGTCTCCGATTGTGGTCAGGTACATCAGGGACCCGAGGAAGTGGAATTTTGCCAAGAAGCCTCTGCTTGTTGTGATGGATCCTCAAGGCAAAATTGTGCACCAGAATGCGGTTCACATGATGTGCATTTGGAGAAGCATCGCCTTCCCTTTCACAAACCAGAGAGAGAGATTGCTTTGGGAAGAAGAAACCTGGAGGATCGAGCTTTTGGCTGATGCCATGGATCCGAACCTAAGTACTTGGATTACGGAAAAGAAGTACATTTGCTTGTATGGCGGAGAAGACATAGAGTGGATCCGAAACTTCACAAAGGCTGCAAGAAACGTGGCTGTTGAAGCCAACATTCAGTTGGAAATGCTATATGTGGGGCGGAGCAAGCCTAAGGAGATCGTGGTGAAGAACATCATGAACATCATCCAAACTGAGAAGCTAAGCCACATCCTTGACTGGAACCTCATTTGGTTCTTCTGGATTCGTCTCGAGAGCATGTGGCAATCCAAAGGACAACTTCTAATGATCAGCCTAGGCTCCCCAGTCATGTCCAGGGCAAATGGAGAGCACATGTTGAAAAGCTTGGAGGAGTTTAAAGACTGGAAAAATAGGGCATCTGAAGTAGGGTTCATGCCAGCCCTGAATGAACACCTAGAAGGGGTGCACAGACAAGCTCCACACCACTGCACCAGTCTTATTTTGCCTGCAACCGGAATCATGCCCGAGACGGTGCAGTGCGCTGAGTGCGGCAGTCTTATGGAGAGGTTCTCCATGTTCCGCTGCTGCCTCGATTCAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0032502 GO:0042802 GO:0042803 GO:0043621 GO:0046983 GO:0048856

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SEO_C SEO_N

Protein Analysis

713

Amino Acids

81.34

Weight (kDa)

6.11

Isoelectric Point (pI)

42.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SEO_N	PF14576	40 - 339	1.1e-66	Sieve element occlusion N-terminus
SEO_C	PF14577	504 - 619	1.3e-47	Sieve element occlusion C-terminus
SEO_C	PF14577	621 - 708	1e-20	Sieve element occlusion C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002043)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g25290 FvH4_4g25290 FvH4_4g25290 FvH4_4g25310 FvH4_4g25320
malus_domestica	MD13G1105000.v1.1 MD13G1105100.v1.1 MD16G1104800.v1.1 MD16G1105000.v1.1
prunus_persica	Prupe.1G241400_v2.0.a1 Prupe.1G241500_v2.0.a1
pyrus_communis	pycom13g09110 pycom13g09120 pycom16g08950 pycom16g08960
rosa_chinensis	RchiOBHm_Chr4g0432561 RchiOBHm_Chr4g0432571 RchiOBHm_Chr4g0432611
rosa_laevigata	RLG00000006831 RLG00000006832 RLG00000006833
rosa_multiflora	Rmu_sc0003886.1_g000001 Rmu_sc0004612.1_g000014 Rmu_sc0027699.1_g000001
rosa_roxburghii	Rroxscaffold_5G00373990 Rroxscaffold_5G00374000 Rroxscaffold_5G00374030
rosa_rugosa	Rorug04G0260100 Rorug04G0260100 Rorug04G0260200
rosa_samantha	Rh4AG315100 Rh4AG315200 Rh4AG315300 Rh4BG322800 Rh4BG322900 Rh4BG323000 Rh4CG337900 Rh4CG338000 Rh4CG338100 Rh4DG318600 Rh4DG318700 Rh4DG318800
rosa_wichuraiana	Rw4G027380 Rw4G027390 Rw4G027420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	10
AccB1I	GGYRCC	1 cut(s)	10
AccBSI	CCGCTC	1 cut(s)	584
AccII	CGCG	6 cut(s)	235, 237, 303, 305, 995, 997
AciI	CCGC	8 cut(s)	305, 584, 626, 1457, 1623, 1717, 2085, 2116
AclWI	GGATC	8 cut(s)	722, 1421, 1434, 1553, 1565, 1578, 1636, 1649
AcoI	YGGCCR	1 cut(s)	52
AcsI	RAATTY	2 cut(s)	204, 1393
AcuI	CTGAAG	3 cut(s)	1136, 1296, 1977
AdeI	CACNNNGTG	2 cut(s)	382, 1330
AfaI	GTAC	7 cut(s)	12, 349, 1175, 1286, 1370, 1585, 1607
AfiI	CCNNNNNNNGG	4 cut(s)	1240, 1456, 1996, 2048
AflII	CTTAAG	1 cut(s)	596
AflIII	ACRYGT	1 cut(s)	1908
AgsI	TTSAA	8 cut(s)	212, 388, 640, 685, 926, 985, 1682, 1915
AhlI	ACTAGT	1 cut(s)	632
AjnI	CCWGG	3 cut(s)	6, 1538, 1889
AleI	CACNNNNGTG	1 cut(s)	419
Alw21I	GWGCWC	4 cut(s)	416, 1449, 1908, 2004
Alw26I	GTCTC	4 cut(s)	165, 1356, 1829, 2057
Alw44I	GTGCAC	3 cut(s)	412, 1445, 2000
AlwI	GGATC	8 cut(s)	722, 1421, 1434, 1553, 1565, 1578, 1636, 1649
Ama87I	CYCGRG	4 cut(s)	274, 1381, 1826, 2060
AoxI	GGCC	6 cut(s)	3, 52, 222, 675, 891, 1223
ApaLI	GTGCAC	3 cut(s)	412, 1445, 2000
ApeKI	GCWGC	6 cut(s)	293, 761, 818, 1661, 2118, 2121
ApoI	RAATTY	2 cut(s)	204, 1393
Asp718I	GGTACC	1 cut(s)	10
AspA2I	CCTAGG	1 cut(s)	1870
AspLEI	GCGC	5 cut(s)	237, 305, 997, 999, 2078
AspS9I	GGNCC	4 cut(s)	4, 675, 892, 1378
AsuC2I	CCSGG	2 cut(s)	275, 276
AsuHPI	GGTGA	4 cut(s)	232, 911, 1328, 1753
AsuII	TTCGAA	1 cut(s)	107
AvaI	CYCGRG	4 cut(s)	274, 1381, 1826, 2060
AvaII	GGWCC	1 cut(s)	1378
AvrII	CCTAGG	1 cut(s)	1870
AxyI	CCTNAGG	1 cut(s)	1728
BaeGI	GKGCMC	3 cut(s)	416, 1449, 2004
BaeI	ACNNNNGTAYC	2 cut(s)	331, 364
BamHI	GGATCC	3 cut(s)	1426, 1570, 1641
BanI	GGYRCC	1 cut(s)	10
BanII	GRGCYC	1 cut(s)	743
BauI	CACGAG	1 cut(s)	545
BbsI	GAAGAC	2 cut(s)	332, 1635
Bbv12I	GWGCWC	4 cut(s)	416, 1449, 1908, 2004
BbvI	GCAGC	6 cut(s)	305, 748, 805, 1648, 2105, 2108
BccI	CCATC	4 cut(s)	177, 254, 524, 1417
BceAI	ACGGC	1 cut(s)	1096
BciT130I	CCWGG	3 cut(s)	8, 1540, 1891
BclI	TGATCA	2 cut(s)	1060, 1863
BcnI	CCSGG	2 cut(s)	275, 276
BcoDI	GTCTC	4 cut(s)	165, 1356, 1829, 2057
BcuI	ACTAGT	1 cut(s)	632
BfaI	CTAG	6 cut(s)	269, 461, 633, 1151, 1871, 1991
BfmI	CTRYAG	2 cut(s)	756, 1344
BfrI	CTTAAG	1 cut(s)	596
BglII	AGATCT	1 cut(s)	612
BisI	GCNGC	7 cut(s)	294, 762, 819, 1662, 2086, 2119, 2122
BlnI	CCTAGG	1 cut(s)	1870
BlpI	GCTNAGC	2 cut(s)	552, 1778
BlsI	GCNGC	7 cut(s)	295, 763, 820, 1663, 2087, 2120, 2123
BmcAI	AGTACT	1 cut(s)	1585
Bme1390I	CCNGG	5 cut(s)	8, 275, 276, 1540, 1891
Bme18I	GGWCC	1 cut(s)	1378
BmeT110I	CYCGRG	4 cut(s)	274, 1381, 1826, 2060
BmgT120I	GGNCC	4 cut(s)	4, 675, 892, 1378
BmrFI	CCNGG	5 cut(s)	8, 275, 276, 1540, 1891
BmrI	ACTGGG	1 cut(s)	1874
BmsI	GCATC	9 cut(s)	298, 424, 643, 830, 1008, 1255, 1494, 1552, 1961
BmuI	ACTGGG	1 cut(s)	1874
BpiI	GAAGAC	2 cut(s)	332, 1635
BplI	GAGNNNNNCTC	2 cut(s)	2090, 2122
BpmI	CTGGAG	1 cut(s)	1561
Bpu1102I	GCTNAGC	2 cut(s)	552, 1778
Bpu14I	TTCGAA	1 cut(s)	107
BpuEI	CTTGAG	1 cut(s)	1416
BpuMI	CCSGG	2 cut(s)	275, 276
BsaJI	CCNNGG	7 cut(s)	6, 274, 507, 1382, 1566, 1870, 1890
BsaWI	WCCGGW	1 cut(s)	2048
Bsc4I	CCNNNNNNNGG	4 cut(s)	1240, 1456, 1996, 2048
Bse118I	RCCGGY	2 cut(s)	50, 289
Bse1I	ACTGG	5 cut(s)	797, 1799, 1880, 1943, 2028
Bse21I	CCTNAGG	1 cut(s)	1728
Bse3DI	GCAATG	1 cut(s)	1325
BseBI	CCWGG	3 cut(s)	8, 1540, 1891
BseDI	CCNNGG	7 cut(s)	6, 274, 507, 1382, 1566, 1870, 1890
BseGI	GGATG	8 cut(s)	169, 252, 313, 493, 1063, 1270, 1761, 1785
BseLI	CCNNNNNNNGG	4 cut(s)	1240, 1456, 1996, 2048
BseMI	GCAATG	1 cut(s)	1325
BseMII	CTCAG	7 cut(s)	335, 566, 785, 861, 1028, 1761, 2070
BseNI	ACTGG	5 cut(s)	797, 1799, 1880, 1943, 2028
BsePI	GCGCGC	1 cut(s)	995
BseRI	GAGGAG	4 cut(s)	57, 60, 177, 1940
BseSI	GKGCMC	3 cut(s)	416, 1449, 2004
BseXI	GCAGC	6 cut(s)	305, 748, 805, 1648, 2105, 2108
BsgI	GTGCAG	3 cut(s)	1053, 2008, 2090
Bsh1236I	CGCG	6 cut(s)	235, 237, 303, 305, 995, 997
BshFI	GGCC	6 cut(s)	5, 54, 224, 677, 893, 1225
BshNI	GGYRCC	1 cut(s)	10
BsiHKAI	GWGCWC	4 cut(s)	416, 1449, 1908, 2004
BsiHKCI	CYCGRG	4 cut(s)	274, 1381, 1826, 2060
BsiSI	CCGG	5 cut(s)	51, 275, 290, 1226, 2049
BslFI	GGGAC	3 cut(s)	257, 643, 1391
BslI	CCNNNNNNNGG	4 cut(s)	1240, 1456, 1996, 2048
BsmAI	GTCTC	4 cut(s)	165, 1356, 1829, 2057
BsmBI	CGTCTC	2 cut(s)	1829, 2057
BsmFI	GGGAC	3 cut(s)	257, 643, 1391
BsmI	GAATGC	2 cut(s)	264, 1459
BsnI	GGCC	6 cut(s)	5, 54, 224, 677, 893, 1225
BsoBI	CYCGRG	4 cut(s)	274, 1381, 1826, 2060
Bsp119I	TTCGAA	1 cut(s)	107
Bsp1286I	GDGCHC	5 cut(s)	416, 743, 1449, 1908, 2004
Bsp1720I	GCTNAGC	2 cut(s)	552, 1778
Bsp19I	CCATGG	1 cut(s)	1566
BspACI	CCGC	8 cut(s)	305, 584, 626, 1457, 1623, 1717, 2085, 2116
BspANI	GGCC	6 cut(s)	5, 54, 224, 677, 893, 1225
BspCNI	CTCAG	7 cut(s)	336, 565, 784, 860, 1029, 1762, 2071
BspFNI	CGCG	6 cut(s)	235, 237, 303, 305, 995, 997
BspHI	TCATGA	2 cut(s)	163, 1752
BspPI	GGATC	8 cut(s)	722, 1421, 1434, 1553, 1565, 1578, 1636, 1649
BspT104I	TTCGAA	1 cut(s)	107
BspT107I	GGYRCC	1 cut(s)	10
BspTI	CTTAAG	1 cut(s)	596
BsrBI	CCGCTC	1 cut(s)	584
BsrDI	GCAATG	1 cut(s)	1325
BsrFI	RCCGGY	2 cut(s)	50, 289
BsrI	ACTGG	5 cut(s)	797, 1799, 1880, 1943, 2028
BssAI	RCCGGY	2 cut(s)	50, 289
BssECI	CCNNGG	7 cut(s)	6, 274, 507, 1382, 1566, 1870, 1890
BssHII	GCGCGC	1 cut(s)	995
BssSI	CACGAG	1 cut(s)	545
BssT1I	CCWWGG	3 cut(s)	507, 1566, 1870
Bst2BI	CACGAG	1 cut(s)	545
Bst2UI	CCWGG	3 cut(s)	8, 1540, 1891
Bst4CI	ACNGT	4 cut(s)	125, 665, 1330, 2068
Bst6I	CTCTTC	3 cut(s)	966, 1088, 1112
BstAFI	CTTAAG	1 cut(s)	596
BstBI	TTCGAA	1 cut(s)	107
BstC8I	GCNNGC	9 cut(s)	52, 504, 891, 997, 1068, 1316, 1724, 1974, 2043
BstDSI	CCRYGG	1 cut(s)	1566
BstF5I	GGATG	8 cut(s)	169, 252, 313, 493, 1063, 1270, 1761, 1785
BstFNI	CGCG	6 cut(s)	235, 237, 303, 305, 995, 997
BstHHI	GCGC	5 cut(s)	237, 305, 997, 999, 2078
BstMAI	GTCTC	4 cut(s)	165, 1356, 1829, 2057
BstNI	CCWGG	3 cut(s)	8, 1540, 1891
BstNSI	RCATGY	3 cut(s)	1318, 1837, 1912
BstSCI	CCNGG	5 cut(s)	6, 273, 274, 1538, 1889
BstSFI	CTRYAG	2 cut(s)	756, 1344
BstSLI	GKGCMC	3 cut(s)	416, 1449, 2004
BstUI	CGCG	6 cut(s)	235, 237, 303, 305, 995, 997
BstV1I	GCAGC	6 cut(s)	305, 748, 805, 1648, 2105, 2108
BstV2I	GAAGAC	2 cut(s)	332, 1635
BstX2I	RGATCY	4 cut(s)	612, 1426, 1570, 1641
BstYI	RGATCY	4 cut(s)	612, 1426, 1570, 1641
Bsu36I	CCTNAGG	1 cut(s)	1728
BsuRI	GGCC	6 cut(s)	5, 54, 224, 677, 893, 1225
BtgI	CCRYGG	1 cut(s)	1566
BtgZI	GCGATG	2 cut(s)	870, 1471
BtsCI	GGATG	8 cut(s)	169, 252, 313, 493, 1063, 1270, 1761, 1785
BtsI	GCAGTG	3 cut(s)	765, 2020, 2078
BtsIMutI	CAGTG	4 cut(s)	130, 765, 2020, 2078
Cac8I	GCNNGC	9 cut(s)	52, 504, 891, 997, 1068, 1316, 1724, 1974, 2043
CciI	TCATGA	2 cut(s)	163, 1752
CfoI	GCGC	5 cut(s)	237, 305, 997, 999, 2078
Cfr10I	RCCGGY	2 cut(s)	50, 289
Cfr13I	GGNCC	4 cut(s)	4, 675, 892, 1378
Cfr9I	CCCGGG	1 cut(s)	274
CseI	GACGC	1 cut(s)	226
Csp6I	GTAC	7 cut(s)	11, 348, 1174, 1285, 1369, 1584, 1606
CspCI	CAANNNNNGTGG	2 cut(s)	1653, 1688
CviQI	GTAC	7 cut(s)	11, 348, 1174, 1285, 1369, 1584, 1606
DraI	TTTAAA	1 cut(s)	1933
DraIII	CACNNNGTG	2 cut(s)	382, 1330
EaeI	YGGCCR	1 cut(s)	52
Eam1104I	CTCTTC	3 cut(s)	966, 1088, 1112
EarI	CTCTTC	3 cut(s)	966, 1088, 1112
EciI	GGCGGA	2 cut(s)	1638, 1732
Eco130I	CCWWGG	3 cut(s)	507, 1566, 1870
Eco24I	GRGCYC	1 cut(s)	743
Eco32I	GATATC	1 cut(s)	931
Eco47I	GGWCC	1 cut(s)	1378
Eco57I	CTGAAG	3 cut(s)	1136, 1296, 1977
Eco81I	CCTNAGG	1 cut(s)	1728
Eco88I	CYCGRG	4 cut(s)	274, 1381, 1826, 2060
EcoO109I	RGGNCCY	1 cut(s)	1378
EcoRII	CCWGG	3 cut(s)	6, 1538, 1889
EcoRV	GATATC	1 cut(s)	931
EcoT14I	CCWWGG	3 cut(s)	507, 1566, 1870
EcoT22I	ATGCAT	2 cut(s)	658, 1270
EcoT38I	GRGCYC	1 cut(s)	743
ErhI	CCWWGG	3 cut(s)	507, 1566, 1870
Esp3I	CGTCTC	2 cut(s)	1829, 2057
FalI	AAGNNNNNCTT	2 cut(s)	1839, 1871
FaqI	GGGAC	3 cut(s)	257, 643, 1391
FauI	CCCGC	1 cut(s)	619
FbaI	TGATCA	2 cut(s)	1060, 1863
Fnu4HI	GCNGC	7 cut(s)	294, 762, 819, 1662, 2086, 2119, 2122
FokI	GGATG	8 cut(s)	156, 239, 320, 500, 1070, 1277, 1748, 1772
FriOI	GRGCYC	1 cut(s)	743
Fsp4HI	GCNGC	7 cut(s)	294, 762, 819, 1662, 2086, 2119, 2122
FspBI	CTAG	6 cut(s)	269, 461, 633, 1151, 1871, 1991
GlaI	GCGC	5 cut(s)	236, 304, 996, 998, 2077
GluI	GCNGC	7 cut(s)	294, 762, 819, 1662, 2086, 2119, 2122
GsuI	CTGGAG	1 cut(s)	1561
HaeIII	GGCC	6 cut(s)	5, 54, 224, 677, 893, 1225
HapII	CCGG	5 cut(s)	51, 275, 290, 1226, 2049
HgaI	GACGC	1 cut(s)	226
HhaI	GCGC	5 cut(s)	237, 305, 997, 999, 2078
Hin6I	GCGC	5 cut(s)	235, 303, 995, 997, 2076
HinP1I	GCGC	5 cut(s)	235, 303, 995, 997, 2076
HincII	GTYRAC	3 cut(s)	31, 136, 1102
HindII	GTYRAC	3 cut(s)	31, 136, 1102
HindIII	AAGCTT	2 cut(s)	978, 1918
HinfI	GANTC	7 cut(s)	718, 837, 1132, 1300, 1819, 2052, 2129
HpaII	CCGG	5 cut(s)	51, 275, 290, 1226, 2049
HphI	GGTGA	4 cut(s)	232, 911, 1328, 1753
Hpy188I	TCNGA	9 cut(s)	403, 774, 850, 1038, 1201, 1356, 1575, 1646, 1957
Hpy188III	TCNNGA	7 cut(s)	164, 958, 1297, 1753, 1816, 1826, 1828
HpyAV	CCTTC	3 cut(s)	1350, 1501, 1988
HpyCH4III	ACNGT	4 cut(s)	125, 665, 1330, 2068
HpyCH4IV	ACGT	3 cut(s)	622, 1155, 1671
HpySE526I	ACGT	3 cut(s)	622, 1155, 1671
HspAI	GCGC	5 cut(s)	235, 303, 995, 997, 2076
KflI	GGGWCCC	1 cut(s)	1378
KpnI	GGTACC	1 cut(s)	14
KroI	GCCGGC	1 cut(s)	50
KroNI	GCCGGC	1 cut(s)	52
Ksp22I	TGATCA	2 cut(s)	1060, 1863
LmnI	GCTCC	7 cut(s)	47, 278, 316, 512, 1719, 1880, 2017
Lsp1109I	GCAGC	6 cut(s)	305, 748, 805, 1648, 2105, 2108
LweI	GCATC	9 cut(s)	298, 424, 643, 830, 1008, 1255, 1494, 1552, 1961
MaeI	CTAG	6 cut(s)	269, 461, 633, 1151, 1871, 1991
MaeII	ACGT	3 cut(s)	622, 1155, 1671
MaeIII	GTNAC	5 cut(s)	86, 196, 238, 688, 862
MbiI	CCGCTC	1 cut(s)	584
MfeI	CAATTG	1 cut(s)	734
MflI	RGATCY	4 cut(s)	612, 1426, 1570, 1641
MhlI	GDGCHC	5 cut(s)	416, 743, 1449, 1908, 2004
MlyI	GAGTC	1 cut(s)	1309
MmeI	TCCRAC	2 cut(s)	568, 1677
Mph1103I	ATGCAT	2 cut(s)	658, 1270
MroNI	GCCGGC	1 cut(s)	50
MseI	TTAA	4 cut(s)	75, 407, 597, 1932
MslI	CAYNNNNRTG	3 cut(s)	419, 1109, 1452
MspA1I	CMGCKG	2 cut(s)	761, 2118
MspCI	CTTAAG	1 cut(s)	596
MspI	CCGG	5 cut(s)	51, 275, 290, 1226, 2049
MspR9I	CCNGG	5 cut(s)	8, 275, 276, 1540, 1891
MunI	CAATTG	1 cut(s)	734
Mva1269I	GAATGC	2 cut(s)	264, 1459
MvaI	CCWGG	3 cut(s)	8, 1540, 1891
MvnI	CGCG	6 cut(s)	235, 237, 303, 305, 995, 997
NaeI	GCCGGC	1 cut(s)	52
NciI	CCSGG	2 cut(s)	275, 276
NcoI	CCATGG	1 cut(s)	1566
NgoMIV	GCCGGC	1 cut(s)	50
NmuCI	GTSAC	5 cut(s)	86, 196, 238, 688, 862
NsiI	ATGCAT	2 cut(s)	658, 1270
NspI	RCATGY	3 cut(s)	1318, 1837, 1912
NspV	TTCGAA	1 cut(s)	107
OliI	CACNNNNGTG	1 cut(s)	419
PaeI	GCATGC	1 cut(s)	1318
PaeR7I	CTCGAG	1 cut(s)	1826
PagI	TCATGA	2 cut(s)	163, 1752
PauI	GCGCGC	1 cut(s)	995
PciI	ACATGT	1 cut(s)	1908
PcsI	WCGNNNNNNNCGW	1 cut(s)	1161
PctI	GAATGC	2 cut(s)	264, 1459
PdiI	GCCGGC	1 cut(s)	52
PfeI	GAWTC	6 cut(s)	718, 837, 1132, 1819, 2052, 2129
PflFI	GACNNNGTC	2 cut(s)	96, 1157
PkrI	GCNGC	7 cut(s)	295, 763, 820, 1663, 2087, 2120, 2123
PleI	GAGTC	1 cut(s)	1308
PpsI	GAGTC	1 cut(s)	1308
PpuMI	RGGWCCY	1 cut(s)	1378
PscI	ACATGT	1 cut(s)	1908
Psp5II	RGGWCCY	1 cut(s)	1378
Psp6I	CCWGG	3 cut(s)	6, 1538, 1889
PspGI	CCWGG	3 cut(s)	6, 1538, 1889
PspPI	GGNCC	4 cut(s)	4, 675, 892, 1378
PspPPI	RGGWCCY	1 cut(s)	1378
PsuI	RGATCY	4 cut(s)	612, 1426, 1570, 1641
PsyI	GACNNNGTC	2 cut(s)	96, 1157
PteI	GCGCGC	1 cut(s)	995
PvuII	CAGCTG	1 cut(s)	761
RsaI	GTAC	7 cut(s)	12, 349, 1175, 1286, 1370, 1585, 1607
RsaNI	GTAC	7 cut(s)	11, 348, 1174, 1285, 1369, 1584, 1606
RseI	CAYNNNNRTG	3 cut(s)	419, 1109, 1452
SaqAI	TTAA	4 cut(s)	75, 407, 597, 1932
SatI	GCNGC	7 cut(s)	294, 762, 819, 1662, 2086, 2119, 2122
Sau96I	GGNCC	4 cut(s)	4, 675, 892, 1378
ScaI	AGTACT	1 cut(s)	1585
SchI	GAGTC	1 cut(s)	1309
ScrFI	CCNGG	5 cut(s)	8, 275, 276, 1540, 1891
SduI	GDGCHC	5 cut(s)	416, 743, 1449, 1908, 2004
SfaNI	GCATC	9 cut(s)	298, 424, 643, 830, 1008, 1255, 1494, 1552, 1961
SfcI	CTRYAG	2 cut(s)	756, 1344
Sfr274I	CTCGAG	1 cut(s)	1826
SfuI	TTCGAA	1 cut(s)	107
SinI	GGWCC	1 cut(s)	1378
SlaI	CTCGAG	1 cut(s)	1826
SmaI	CCCGGG	1 cut(s)	276
SmiMI	CAYNNNNRTG	3 cut(s)	419, 1109, 1452
SmlI	CTYRAG	3 cut(s)	596, 1431, 1826
SmoI	CTYRAG	3 cut(s)	596, 1431, 1826
SpeI	ACTAGT	1 cut(s)	632
SphI	GCATGC	1 cut(s)	1318
SsiI	CCGC	8 cut(s)	305, 584, 626, 1457, 1623, 1717, 2085, 2116
SspMI	CTAG	6 cut(s)	269, 461, 633, 1151, 1871, 1991
StyD4I	CCNGG	5 cut(s)	6, 273, 274, 1538, 1889
StyI	CCWWGG	3 cut(s)	507, 1566, 1870
TaaI	ACNGT	4 cut(s)	125, 665, 1330, 2068
TaiI	ACGT	3 cut(s)	625, 1158, 1674
TaqI	TCGA	9 cut(s)	107, 651, 915, 936, 1028, 1296, 1548, 1827, 2127
TatI	WGTACW	4 cut(s)	1173, 1284, 1583, 1605
TauI	GCSGC	1 cut(s)	2088
TfiI	GAWTC	6 cut(s)	718, 837, 1132, 1819, 2052, 2129
Tru1I	TTAA	4 cut(s)	75, 407, 597, 1932
Tru9I	TTAA	4 cut(s)	75, 407, 597, 1932
TscAI	CASTG	4 cut(s)	130, 772, 2027, 2078
TseFI	GTSAC	5 cut(s)	86, 196, 238, 688, 862
TseI	GCWGC	6 cut(s)	293, 761, 818, 1661, 2118, 2121
Tsp45I	GTSAC	5 cut(s)	86, 196, 238, 688, 862
TspDTI	ATGAA	6 cut(s)	504, 1129, 1208, 1769, 1957, 1998
TspGWI	ACGGA	3 cut(s)	105, 1277, 1610
TspMI	CCCGGG	1 cut(s)	274
TspRI	CASTG	4 cut(s)	130, 772, 2027, 2078
Tth111I	GACNNNGTC	2 cut(s)	96, 1157
Vha464I	CTTAAG	1 cut(s)	596
VneI	GTGCAC	3 cut(s)	412, 1445, 2000
VpaK11BI	GGWCC	1 cut(s)	1378
XapI	RAATTY	2 cut(s)	204, 1393
XceI	RCATGY	3 cut(s)	1318, 1837, 1912
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1693
XhoI	CTCGAG	1 cut(s)	1826
XmaI	CCCGGG	1 cut(s)	274
XmaJI	CCTAGG	1 cut(s)	1870
XspI	CTAG	6 cut(s)	269, 461, 633, 1151, 1871, 1991
ZrmI	AGTACT	1 cut(s)	1585
Zsp2I	ATGCAT	2 cut(s)	658, 1270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.