pycom16g12580

Pectinesterase inhibitor-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005409
Physical Location & Seq
Forward (+)
8862436 .. 8862975
540 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGAGAAAAATGTGTTCTTCAGTTTGTAACCCTAGCTTCATTTGCATTCTGCTAATCTCTTTCTTGATCGATGATCCTTCTCAGGCATTTGATCTCAATTCTATATGCAAACAATCCATGAACCCGTCATTTTGTTCTCTGCTCTTAAGTTCGAATCCCCGCGTGACTGAGGCACTTCTTCGCAGCCTGGCCGGTGTCACCGTAGATTTGGGATATTCCAATACTACAGCCACTAGAGATCACATAGTAAAGTGGAAGAACCGGACGGACAATCCAGCGTTGCGGCTGAGGTACACATCTTGTGCCGGAAATTACAACGAGGCCGTTGCAAATTTCAAGGAGGCCAAGGAGAAAGCGAAGGTTGGTGACTACAATGGTGTGAGAAATGCAGCAGCTGGTGCCTTGGGAGAGTTCAATGGGTGTTCAGAGAACTTTAAGCAGCCACCATCTGAACCAACAAGTCTCTGGCAAGATACTAAGGATTTGCTAAATCTTTCTAGTATTATATTAATTGTTTCTAATACTTTGCTTCACAAATGA

Protein Analysis

180

Amino Acids

19.67

Weight (kDa)

8.47

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 170 9.5e-14 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 398
AccII CGCG 1 cut(s) 162
AciI CCGC 2 cut(s) 160, 283
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 1 cut(s) 331
AfaI GTAC 1 cut(s) 293
AflII CTTAAG 1 cut(s) 145
AgsI TTSAA 2 cut(s) 337, 415
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 2 cut(s) 36, 395
AluI AGCT 2 cut(s) 36, 395
Alw26I GTCTC 1 cut(s) 467
AlwI GGATC 1 cut(s) 68
AlwNI CAGNNNCTG 1 cut(s) 395
AoxI GGCC 3 cut(s) 189, 321, 342
ApeKI GCWGC 4 cut(s) 183, 389, 392, 439
ApoI RAATTY 1 cut(s) 331
AseI ATTAAT 1 cut(s) 509
AsuHPI GGTGA 2 cut(s) 190, 377
AsuII TTCGAA 1 cut(s) 152
BanI GGYRCC 1 cut(s) 398
BbvCI CCTCAGC 1 cut(s) 287
BbvI GCAGC 4 cut(s) 195, 401, 404, 451
BccI CCATC 1 cut(s) 454
BceAI ACGGC 1 cut(s) 308
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 467
BfaI CTAG 3 cut(s) 33, 234, 498
BfmI CTRYAG 1 cut(s) 225
BfrI CTTAAG 1 cut(s) 145
BisI GCNGC 5 cut(s) 184, 284, 390, 393, 440
BlsI GCNGC 5 cut(s) 185, 285, 391, 394, 441
Bme1390I CCNGG 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 287
Bpu14I TTCGAA 1 cut(s) 152
Bsa29I ATCGAT 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 345, 402
BsaWI WCCGGW 1 cut(s) 261
Bse118I RCCGGY 1 cut(s) 191
BseBI CCWGG 1 cut(s) 188
BseCI ATCGAT 1 cut(s) 69
BseDI CCNNGG 2 cut(s) 345, 402
BseMII CTCAG 3 cut(s) 95, 159, 278
BseXI GCAGC 4 cut(s) 195, 401, 404, 451
Bsh1236I CGCG 1 cut(s) 162
BshFI GGCC 3 cut(s) 191, 323, 344
BshNI GGYRCC 1 cut(s) 398
BshVI ATCGAT 1 cut(s) 69
BsiSI CCGG 3 cut(s) 192, 262, 306
BsmAI GTCTC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 45
BsnI GGCC 3 cut(s) 191, 323, 344
Bsp119I TTCGAA 1 cut(s) 152
Bsp143I GATC 4 cut(s) 66, 73, 91, 238
BspACI CCGC 2 cut(s) 160, 283
BspANI GGCC 3 cut(s) 191, 323, 344
BspCNI CTCAG 3 cut(s) 94, 160, 279
BspDI ATCGAT 1 cut(s) 69
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 400
BspPI GGATC 1 cut(s) 68
BspT104I TTCGAA 1 cut(s) 152
BspT107I GGYRCC 1 cut(s) 398
BspTI CTTAAG 1 cut(s) 145
BsrFI RCCGGY 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 2 cut(s) 345, 402
BssMI GATC 4 cut(s) 66, 73, 91, 238
BssT1I CCWWGG 2 cut(s) 345, 402
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 1 cut(s) 202
BstAFI CTTAAG 1 cut(s) 145
BstAPI GCANNNNNTGC 1 cut(s) 398
BstBI TTCGAA 1 cut(s) 152
BstDEI CTNAG 4 cut(s) 81, 168, 287, 477
BstFNI CGCG 1 cut(s) 162
BstKTI GATC 4 cut(s) 69, 76, 94, 241
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 4 cut(s) 66, 73, 91, 238
BstMWI GCNNNNNNNGC 2 cut(s) 42, 398
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 186
BstSFI CTRYAG 1 cut(s) 225
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 4 cut(s) 195, 401, 404, 451
Bsu15I ATCGAT 1 cut(s) 69
BsuRI GGCC 3 cut(s) 191, 323, 344
BsuTUI ATCGAT 1 cut(s) 69
CaiI CAGNNNCTG 1 cut(s) 395
Cfr10I RCCGGY 1 cut(s) 191
ClaI ATCGAT 1 cut(s) 69
Csp6I GTAC 1 cut(s) 292
CviAII CATG 1 cut(s) 118
CviJI RGCY 9 cut(s) 36, 186, 191, 230, 286, 323, 344, 395, 442
CviKI_1 RGCY 9 cut(s) 36, 186, 191, 230, 286, 323, 344, 395, 442
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 4 cut(s) 81, 168, 287, 477
DpnI GATC 4 cut(s) 68, 75, 93, 240
DpnII GATC 4 cut(s) 66, 73, 91, 238
EaeI YGGCCR 1 cut(s) 189
Eco130I CCWWGG 2 cut(s) 345, 402
EcoRII CCWGG 1 cut(s) 186
EcoT14I CCWWGG 2 cut(s) 345, 402
ErhI CCWWGG 2 cut(s) 345, 402
FaeI CATG 1 cut(s) 121
FaiI YATR 5 cut(s) 104, 106, 119, 245, 506
FatI CATG 1 cut(s) 117
FauI CCCGC 1 cut(s) 167
Fnu4HI GCNGC 5 cut(s) 184, 284, 390, 393, 440
Fsp4HI GCNGC 5 cut(s) 184, 284, 390, 393, 440
FspBI CTAG 3 cut(s) 33, 234, 498
GluI GCNGC 5 cut(s) 184, 284, 390, 393, 440
HaeIII GGCC 3 cut(s) 191, 323, 344
HapII CCGG 3 cut(s) 192, 262, 306
Hin1II CATG 1 cut(s) 121
HinfI GANTC 1 cut(s) 154
HpaII CCGG 3 cut(s) 192, 262, 306
HphI GGTGA 2 cut(s) 190, 377
Hpy166II GTNNAC 1 cut(s) 294
Hpy188I TCNGA 2 cut(s) 427, 451
Hpy188III TCNNGA 1 cut(s) 64
Hpy8I GTNNAC 1 cut(s) 294
HpyAV CCTTC 2 cut(s) 87, 352
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4V TGCA 4 cut(s) 45, 108, 329, 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 398
HpyF3I CTNAG 4 cut(s) 81, 168, 287, 477
Hsp92II CATG 1 cut(s) 121
Kzo9I GATC 4 cut(s) 66, 73, 91, 238
LpnPI CCDG 9 cut(s) 68, 173, 200, 205, 275, 288, 319, 381, 451
Lsp1109I GCAGC 4 cut(s) 195, 401, 404, 451
MaeI CTAG 3 cut(s) 33, 234, 498
MaeIII GTNAC 4 cut(s) 26, 163, 196, 365
MalI GATC 4 cut(s) 68, 75, 93, 240
MboI GATC 4 cut(s) 66, 73, 91, 238
MboII GAAGA 3 cut(s) 9, 170, 268
MluCI AATT 4 cut(s) 97, 310, 331, 510
MnlI CCTC 4 cut(s) 163, 282, 313, 334
MseI TTAA 3 cut(s) 146, 435, 509
MspA1I CMGCKG 1 cut(s) 395
MspCI CTTAAG 1 cut(s) 145
MspI CCGG 3 cut(s) 192, 262, 306
MspR9I CCNGG 1 cut(s) 188
Mva1269I GAATGC 1 cut(s) 45
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 2 cut(s) 42, 398
NdeII GATC 4 cut(s) 66, 73, 91, 238
NlaIII CATG 1 cut(s) 121
NlaIV GGNNCC 1 cut(s) 400
NmuCI GTSAC 3 cut(s) 163, 196, 365
NspV TTCGAA 1 cut(s) 152
PctI GAATGC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 154
PkrI GCNGC 5 cut(s) 185, 285, 391, 394, 441
PshBI ATTAAT 1 cut(s) 509
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 400
PstNI CAGNNNCTG 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
SaqAI TTAA 3 cut(s) 146, 435, 509
SatI GCNGC 5 cut(s) 184, 284, 390, 393, 440
Sau3AI GATC 4 cut(s) 66, 73, 91, 238
ScrFI CCNGG 1 cut(s) 188
SetI ASST 4 cut(s) 38, 293, 363, 397
SfcI CTRYAG 1 cut(s) 225
SfuI TTCGAA 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 4 cut(s) 97, 310, 331, 510
SsiI CCGC 2 cut(s) 160, 283
SspMI CTAG 3 cut(s) 33, 234, 498
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 2 cut(s) 345, 402
TaaI ACNGT 1 cut(s) 202
TaqI TCGA 2 cut(s) 69, 152
TasI AATT 4 cut(s) 97, 310, 331, 510
TauI GCSGC 1 cut(s) 286
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 3 cut(s) 146, 435, 509
Tru9I TTAA 3 cut(s) 146, 435, 509
TseFI GTSAC 3 cut(s) 163, 196, 365
TseI GCWGC 4 cut(s) 183, 389, 392, 439
Tsp45I GTSAC 3 cut(s) 163, 196, 365
TspDTI ATGAA 2 cut(s) 28, 134
TspGWI ACGGA 1 cut(s) 281
Vha464I CTTAAG 1 cut(s) 145
VspI ATTAAT 1 cut(s) 509
XapI RAATTY 1 cut(s) 331
XcmI CCANNNNNNNNNTGG 1 cut(s) 462
XspI CTAG 3 cut(s) 33, 234, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.