Rh4DG267200

Pectinesterase inhibitor-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005409
Physical Location & Seq
Forward (+)
48656251 .. 48659394
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG267200.1

Sequence Viewer

Length: 705 bp
ATGGTTTCTTCTTCTCCCTCAGTAGCTTGCATAGCTGTAATGTCTTTCATGGTTCTGATGAGCACTACTCCCTCCTCTGCTTTTATCCTAAACAAAGATATTTGCTCCAACACCAAGGTCTTGAGTCGCTCTTTTTGTTCGAAATTTCTGGGTTCTAATCCGGTTGTGATCAAGTCCCTCCTTCTCAGCCTCGCTGAGGCCACTATGGATGTCGCATCTTCGAACGCTACAAAGACCAGCCAGCTGATAATCAAGTGGCAAAACCAAACAGACGATCCTCAACTGAAGAATGCAATTAGTCTATGTCTGAGGCCGTACAGTGAAGCCATTACTGATCTCAAGGAAGCAAAAGAGAAGGTCAGGTCTGGGGACTATGTTGCACTACAACGTCCAGTAGTTGATGCTTCTGTAAAGTTTCATGCATGCGAGGAGAAATTCGAGGACACACCACCTGATCCATCGCCATTGTGCAGTACTAGTGGTGAATTAGCTGCCATCTCTAGCATTCTTATAGCTGTTTCTGAGTTTCTGGCCGTATTGCTGCTCTCAAGGATGCCAAAGAAGGTCAGGTCTGGTAACTATAATGTAAACCTTGCAGCAGTTGAGGCCTCCACAAAGTTTAATGCGTGTGAAGACCGATTTGATGAGGACATATCTGATCCATCACCATTAGCAAGTGATAAAAACGATATAGCCACTCTCTAA

Protein Analysis

234

Amino Acids

25.25

Weight (kDa)

5.15

Isoelectric Point (pI)

52.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 172 8.9e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 269, 449, 653
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 2 cut(s) 143, 434
AcuI CTGAAG 1 cut(s) 305
AfaI GTAC 2 cut(s) 317, 475
AfiI CCNNNNNNNGG 1 cut(s) 196
AhlI ACTAGT 1 cut(s) 476
AluBI AGCT 5 cut(s) 26, 35, 244, 491, 515
AluI AGCT 5 cut(s) 26, 35, 244, 491, 515
Alw21I GWGCWC 1 cut(s) 65
AlwI GGATC 3 cut(s) 269, 449, 653
AoxI GGCC 4 cut(s) 198, 311, 531, 606
ApeKI GCWGC 3 cut(s) 491, 541, 596
ApoI RAATTY 2 cut(s) 143, 434
AsuHPI GGTGA 2 cut(s) 494, 657
AsuII TTCGAA 2 cut(s) 140, 221
BbsI GAAGAC 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 65
BbvCI CCTCAGC 1 cut(s) 195
BbvI GCAGC 3 cut(s) 478, 528, 608
BccI CCATC 3 cut(s) 466, 503, 670
BceAI ACGGC 2 cut(s) 298, 518
BclI TGATCA 1 cut(s) 168
BcuI ACTAGT 1 cut(s) 476
BfaI CTAG 2 cut(s) 477, 501
BisI GCNGC 3 cut(s) 492, 542, 597
BlsI GCNGC 3 cut(s) 493, 543, 598
BmcAI AGTACT 1 cut(s) 475
BmsI GCATC 3 cut(s) 224, 391, 543
BpiI GAAGAC 1 cut(s) 639
BplI GAGNNNNNCTC 2 cut(s) 52, 84
Bpu10I CCTNAGC 1 cut(s) 195
Bpu14I TTCGAA 2 cut(s) 140, 221
BpuEI CTTGAG 3 cut(s) 142, 323, 532
BsaJI CCNNGG 1 cut(s) 114
BsaWI WCCGGW 1 cut(s) 160
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 392
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 2 cut(s) 214, 558
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 5 cut(s) 33, 186, 199, 299, 513
BseNI ACTGG 1 cut(s) 392
BseRI GAGGAG 2 cut(s) 64, 443
BseXI GCAGC 3 cut(s) 478, 528, 608
BsgI GTGCAG 1 cut(s) 490
BshFI GGCC 4 cut(s) 200, 313, 533, 608
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 2 cut(s) 160, 383
BslI CCNNNNNNNGG 1 cut(s) 196
BsmFI GGGAC 2 cut(s) 160, 383
BsmI GAATGC 2 cut(s) 295, 504
BsnI GGCC 4 cut(s) 200, 313, 533, 608
Bsp119I TTCGAA 2 cut(s) 140, 221
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 5 cut(s) 168, 274, 334, 454, 658
BspANI GGCC 4 cut(s) 200, 313, 533, 608
BspCNI CTCAG 5 cut(s) 32, 187, 198, 300, 514
BspPI GGATC 3 cut(s) 269, 449, 653
BspT104I TTCGAA 2 cut(s) 140, 221
BsrI ACTGG 1 cut(s) 392
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 5 cut(s) 168, 274, 334, 454, 658
BssT1I CCWWGG 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 320
BstBI TTCGAA 2 cut(s) 140, 221
BstC8I GCNNGC 3 cut(s) 28, 242, 424
BstDEI CTNAG 5 cut(s) 19, 185, 195, 308, 522
BstENI CCTNNNNNAGG 1 cut(s) 194
BstF5I GGATG 2 cut(s) 214, 558
BstKTI GATC 5 cut(s) 171, 277, 337, 457, 661
BstMBI GATC 5 cut(s) 168, 274, 334, 454, 658
BstMWI GCNNNNNNNGC 2 cut(s) 32, 605
BstNSI RCATGY 1 cut(s) 426
BstV1I GCAGC 3 cut(s) 478, 528, 608
BstV2I GAAGAC 1 cut(s) 639
BsuRI GGCC 4 cut(s) 200, 313, 533, 608
BtgZI GCGATG 1 cut(s) 444
BtsCI GGATG 2 cut(s) 214, 558
BtsIMutI CAGTG 1 cut(s) 325
Cac8I GCNNGC 3 cut(s) 28, 242, 424
Csp6I GTAC 2 cut(s) 316, 474
CviAII CATG 3 cut(s) 49, 419, 423
CviQI GTAC 2 cut(s) 316, 474
DdeI CTNAG 5 cut(s) 19, 185, 195, 308, 522
DpnI GATC 5 cut(s) 170, 276, 336, 456, 660
DpnII GATC 5 cut(s) 168, 274, 334, 454, 658
EaeI YGGCCR 1 cut(s) 531
Eco130I CCWWGG 1 cut(s) 114
Eco147I AGGCCT 1 cut(s) 608
Eco57I CTGAAG 1 cut(s) 305
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 114
EcoT22I ATGCAT 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 3 cut(s) 52, 422, 426
FaqI GGGAC 2 cut(s) 160, 383
FatI CATG 3 cut(s) 48, 418, 422
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 3 cut(s) 492, 542, 597
FokI GGATG 2 cut(s) 221, 565
Fsp4HI GCNGC 3 cut(s) 492, 542, 597
FspBI CTAG 2 cut(s) 477, 501
GluI GCNGC 3 cut(s) 492, 542, 597
HaeIII GGCC 4 cut(s) 200, 313, 533, 608
HapII CCGG 1 cut(s) 161
Hin1II CATG 3 cut(s) 52, 422, 426
HinfI GANTC 1 cut(s) 124
HpaII CCGG 1 cut(s) 161
HphI GGTGA 2 cut(s) 494, 657
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 4 cut(s) 57, 309, 523, 658
Hpy188III TCNNGA 1 cut(s) 121
Hpy8I GTNNAC 1 cut(s) 589
HpyAV CCTTC 3 cut(s) 191, 349, 556
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 6 cut(s) 30, 293, 380, 422, 471, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 605
HpyF3I CTNAG 5 cut(s) 19, 185, 195, 308, 522
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 3 cut(s) 52, 422, 426
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 5 cut(s) 168, 274, 334, 454, 658
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 3 cut(s) 478, 528, 608
LweI GCATC 3 cut(s) 224, 391, 543
MaeI CTAG 2 cut(s) 477, 501
MaeII ACGT 1 cut(s) 388
MaeIII GTNAC 1 cut(s) 575
MalI GATC 5 cut(s) 170, 276, 336, 456, 660
MboI GATC 5 cut(s) 168, 274, 334, 454, 658
MboII GAAGA 4 cut(s) 3, 210, 298, 644
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 4 cut(s) 143, 294, 434, 485
MlyI GAGTC 1 cut(s) 133
MmeI TCCRAC 1 cut(s) 132
Mph1103I ATGCAT 1 cut(s) 424
MseI TTAA 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 244
MspI CCGG 1 cut(s) 161
Mva1269I GAATGC 2 cut(s) 295, 504
MwoI GCNNNNNNNGC 2 cut(s) 32, 605
NdeII GATC 5 cut(s) 168, 274, 334, 454, 658
NlaIII CATG 3 cut(s) 52, 422, 426
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 426
NspV TTCGAA 2 cut(s) 140, 221
PaeI GCATGC 1 cut(s) 426
PceI AGGCCT 1 cut(s) 608
PctI GAATGC 2 cut(s) 295, 504
PkrI GCNGC 3 cut(s) 493, 543, 598
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PvuII CAGCTG 1 cut(s) 244
RsaI GTAC 2 cut(s) 317, 475
RsaNI GTAC 2 cut(s) 316, 474
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 3 cut(s) 492, 542, 597
Sau3AI GATC 5 cut(s) 168, 274, 334, 454, 658
ScaI AGTACT 1 cut(s) 475
SchI GAGTC 1 cut(s) 133
SduI GDGCHC 1 cut(s) 65
SfaNI GCATC 3 cut(s) 224, 391, 543
SfuI TTCGAA 2 cut(s) 140, 221
SmlI CTYRAG 3 cut(s) 121, 338, 547
SmoI CTYRAG 3 cut(s) 121, 338, 547
SpeI ACTAGT 1 cut(s) 476
SphI GCATGC 1 cut(s) 426
Sse9I AATT 4 cut(s) 143, 294, 434, 485
SseBI AGGCCT 1 cut(s) 608
SspMI CTAG 2 cut(s) 477, 501
StuI AGGCCT 1 cut(s) 608
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 1 cut(s) 391
TaqI TCGA 3 cut(s) 140, 221, 438
TaqII GACCGA 1 cut(s) 651
TasI AATT 4 cut(s) 143, 294, 434, 485
TatI WGTACW 1 cut(s) 473
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TscAI CASTG 1 cut(s) 325
TseI GCWGC 3 cut(s) 491, 541, 596
TspDTI ATGAA 2 cut(s) 37, 407
TspRI CASTG 1 cut(s) 325
XagI CCTNNNNNAGG 1 cut(s) 194
XapI RAATTY 2 cut(s) 143, 434
XceI RCATGY 1 cut(s) 426
XspI CTAG 2 cut(s) 477, 501
ZrmI AGTACT 1 cut(s) 475
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.