RLG00000007305

Pectinesterase inhibitor-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005409
Physical Location & Seq
Forward (+)
15578059 .. 15578598
540 bp
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UTR
Exon/CDS
Intron
RLM00000007305

Sequence Viewer

Length: 540 bp
ATGGCTTCTTCTTCTTCCTCAATAGCTTGCATTGCTGTAATGGTTCTGATGAGTACTACTCCCTCCTCTGCTTTTACCCTAAACAAGGATATTTGCTCCAACACCAAAGTCTTGAGTCGCTCTTTCTGTTCGCAATTTCTGGGTTCTAATCCGGTTGTGATCAAGTCCCTCCTTCTCAGCCTCGCCGAGGCCACTATAGATGTTGCATCTTCGAACGCTACGAAGACCAGCCAGCTGATAATCAAGTGGCAAAACCAAACAGACAATCCCCAACTGAAGAATGCAATTAGACAATGTCCGACTCCCTACAATGAAGCCATTGCTGATCTCAGGGAAGCCAAAGAGAAGGTCAGGTCTGGTGACTATGTTGCACTACAACGTGCAGCAGTTGATGCTTCTATAAAGTTTCATGCATGCGAAGAGAAATTTGAGGACACGCCACCTGATCCATCACCATTGTGCAGTAATAGTGGTGAATTAGCTGCTATTTCTAGCATCCTCATAGCTGTATTTGAAATTCTGGCAAAATCATACAAGTGA

Protein Analysis

180

Amino Acids

19.25

Weight (kDa)

6.57

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 29 - 169 3.9e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 440
AcsI RAATTY 2 cut(s) 425, 516
AcuI CTGAAG 1 cut(s) 296
AfaI GTAC 1 cut(s) 55
AfiI CCNNNNNNNGG 2 cut(s) 85, 187
AgsI TTSAA 1 cut(s) 515
AleI CACNNNNGTG 1 cut(s) 457
AluBI AGCT 4 cut(s) 26, 235, 482, 506
AluI AGCT 4 cut(s) 26, 235, 482, 506
AlwI GGATC 1 cut(s) 440
AoxI GGCC 1 cut(s) 189
ApeKI GCWGC 2 cut(s) 383, 482
ApoI RAATTY 2 cut(s) 425, 516
ArsI GACNNNNNNTTYG 2 cut(s) 333, 365
AsuHPI GGTGA 3 cut(s) 371, 444, 485
AsuII TTCGAA 1 cut(s) 212
BbsI GAAGAC 1 cut(s) 230
BbvI GCAGC 2 cut(s) 395, 469
BccI CCATC 1 cut(s) 457
BclI TGATCA 1 cut(s) 159
BfaI CTAG 1 cut(s) 492
BfmI CTRYAG 1 cut(s) 195
BisI GCNGC 2 cut(s) 384, 483
BlsI GCNGC 2 cut(s) 385, 484
BmcAI AGTACT 1 cut(s) 55
BmsI GCATC 3 cut(s) 215, 382, 504
BpiI GAAGAC 1 cut(s) 230
BplI GAGNNNNNCTC 2 cut(s) 43, 75
Bpu14I TTCGAA 1 cut(s) 212
BpuEI CTTGAG 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 187
Bse3DI GCAATG 2 cut(s) 30, 318
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 495
BseLI CCNNNNNNNGG 2 cut(s) 85, 187
BseMI GCAATG 2 cut(s) 30, 318
BseMII CTCAG 2 cut(s) 190, 343
BseRI GAGGAG 1 cut(s) 55
BseXI GCAGC 2 cut(s) 395, 469
BsgI GTGCAG 2 cut(s) 402, 481
BshFI GGCC 1 cut(s) 191
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 2 cut(s) 85, 187
BsmFI GGGAC 1 cut(s) 151
BsmI GAATGC 1 cut(s) 286
BsnI GGCC 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 212
Bsp143I GATC 3 cut(s) 159, 325, 445
BspANI GGCC 1 cut(s) 191
BspCNI CTCAG 2 cut(s) 189, 342
BspPI GGATC 1 cut(s) 440
BspT104I TTCGAA 1 cut(s) 212
BsrDI GCAATG 2 cut(s) 30, 318
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 3 cut(s) 159, 325, 445
Bst6I CTCTTC 1 cut(s) 414
BstAPI GCANNNNNTGC 1 cut(s) 392
BstBI TTCGAA 1 cut(s) 212
BstC8I GCNNGC 3 cut(s) 28, 233, 415
BstDEI CTNAG 2 cut(s) 176, 329
BstENI CCTNNNNNAGG 2 cut(s) 83, 185
BstF5I GGATG 1 cut(s) 495
BstKTI GATC 3 cut(s) 162, 328, 448
BstMBI GATC 3 cut(s) 159, 325, 445
BstMWI GCNNNNNNNGC 2 cut(s) 32, 392
BstNSI RCATGY 1 cut(s) 417
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 395, 469
BstV2I GAAGAC 1 cut(s) 230
BsuRI GGCC 1 cut(s) 191
BtsCI GGATG 1 cut(s) 495
Cac8I GCNNGC 3 cut(s) 28, 233, 415
Csp6I GTAC 1 cut(s) 54
CviAII CATG 2 cut(s) 410, 414
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 2 cut(s) 176, 329
DpnI GATC 3 cut(s) 161, 327, 447
DpnII GATC 3 cut(s) 159, 325, 445
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco57I CTGAAG 1 cut(s) 296
EcoNI CCTNNNNNAGG 2 cut(s) 83, 185
EcoT22I ATGCAT 1 cut(s) 415
FaeI CATG 2 cut(s) 413, 417
FaiI YATR 7 cut(s) 197, 366, 401, 411, 415, 503, 532
FaqI GGGAC 1 cut(s) 151
FatI CATG 2 cut(s) 409, 413
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 2 cut(s) 384, 483
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 2 cut(s) 384, 483
FspBI CTAG 1 cut(s) 492
GluI GCNGC 2 cut(s) 384, 483
HaeIII GGCC 1 cut(s) 191
HapII CCGG 1 cut(s) 152
Hin1II CATG 2 cut(s) 413, 417
HinfI GANTC 2 cut(s) 115, 301
HpaII CCGG 1 cut(s) 152
HphI GGTGA 3 cut(s) 371, 444, 485
Hpy188I TCNGA 2 cut(s) 48, 300
Hpy188III TCNNGA 1 cut(s) 112
HpyAV CCTTC 2 cut(s) 182, 340
HpyCH4IV ACGT 1 cut(s) 379
HpyCH4V TGCA 7 cut(s) 30, 206, 284, 371, 383, 413, 462
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 392
HpyF3I CTNAG 2 cut(s) 176, 329
HpySE526I ACGT 1 cut(s) 379
Hsp92II CATG 2 cut(s) 413, 417
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 3 cut(s) 159, 325, 445
LmnI GCTCC 1 cut(s) 101
LpnPI CCDG 9 cut(s) 125, 165, 241, 245, 316, 337, 342, 456, 506
Lsp1109I GCAGC 2 cut(s) 395, 469
LweI GCATC 3 cut(s) 215, 382, 504
MaeI CTAG 1 cut(s) 492
MaeII ACGT 1 cut(s) 379
MaeIII GTNAC 1 cut(s) 359
MalI GATC 3 cut(s) 161, 327, 447
MboI GATC 3 cut(s) 159, 325, 445
MboII GAAGA 6 cut(s) 3, 6, 201, 235, 289, 431
MluCI AATT 5 cut(s) 134, 285, 425, 476, 516
MlyI GAGTC 2 cut(s) 124, 295
MmeI TCCRAC 2 cut(s) 123, 323
MnlI CCTC 8 cut(s) 28, 73, 76, 179, 181, 191, 424, 509
Mph1103I ATGCAT 1 cut(s) 415
MslI CAYNNNNRTG 2 cut(s) 457, 535
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 1 cut(s) 152
Mva1269I GAATGC 1 cut(s) 286
MwoI GCNNNNNNNGC 2 cut(s) 32, 392
NdeII GATC 3 cut(s) 159, 325, 445
NlaIII CATG 2 cut(s) 413, 417
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 359
NsiI ATGCAT 1 cut(s) 415
NspI RCATGY 1 cut(s) 417
NspV TTCGAA 1 cut(s) 212
OliI CACNNNNGTG 1 cut(s) 457
PaeI GCATGC 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 218
PctI GAATGC 1 cut(s) 286
PflFI GACNNNGTC 1 cut(s) 294
PkrI GCNGC 2 cut(s) 385, 484
PleI GAGTC 2 cut(s) 123, 295
PpsI GAGTC 2 cut(s) 123, 295
PsyI GACNNNGTC 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
RseI CAYNNNNRTG 2 cut(s) 457, 535
SatI GCNGC 2 cut(s) 384, 483
Sau3AI GATC 3 cut(s) 159, 325, 445
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 2 cut(s) 124, 295
SetI ASST 8 cut(s) 28, 237, 351, 356, 382, 445, 484, 508
SfaNI GCATC 3 cut(s) 215, 382, 504
SfcI CTRYAG 1 cut(s) 195
SfuI TTCGAA 1 cut(s) 212
SmiMI CAYNNNNRTG 2 cut(s) 457, 535
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
SphI GCATGC 1 cut(s) 417
Sse9I AATT 5 cut(s) 134, 285, 425, 476, 516
SspMI CTAG 1 cut(s) 492
TaiI ACGT 1 cut(s) 382
TaqI TCGA 1 cut(s) 212
TasI AATT 5 cut(s) 134, 285, 425, 476, 516
TatI WGTACW 1 cut(s) 53
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 2 cut(s) 383, 482
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 2 cut(s) 327, 398
Tth111I GACNNNGTC 1 cut(s) 294
XagI CCTNNNNNAGG 2 cut(s) 83, 185
XapI RAATTY 2 cut(s) 425, 516
XceI RCATGY 1 cut(s) 417
XspI CTAG 1 cut(s) 492
ZrmI AGTACT 1 cut(s) 55
Zsp2I ATGCAT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.