RLG00000007327

Pectinesterase inhibitor-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005409
Physical Location & Seq
Reverse (-)
15852778 .. 15855900
3123 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007327

Sequence Viewer

Length: 663 bp
ATGTCTTTCATGGTTCTGATGAGCACTACTCCCTCCTTTGCTTTTATCCTAAACAAAGATATTTGCTCCAACACCAAGGTCTTGAGTCGCTCTTTTTGTTCGAAATTTCTGGGTTCTAATCCGGTTGTGATCAAGTCCCTCCTTCTCAGCCTCACTGAGGCCACTATGGATGTCGCGTCTTCGAACGCTACAAAGACCAGCCAGCTGATAATCAAGTGGCAAAACCAAACAGACAATCCTCAGCTGAAGAATGCAATTAGTCTATGTCCGAGGCCGTACAGTGAAGCCATTACTGATCTCAAGGAAGCAAAAGAGAAGGTCAGGTCTGGGGACTATGTTGCACTACAACGTGCAGTAGTTGATGCTTCTATAAAGTTTCATGCATGCGAGGAGAAATTCGAGGACACGCCACCTGATCCATCGCCATTGTGCAGTACTAGTGGTGAATTAGCTGCCATCTCTAGCATTCTTATAGCTGTTTCCGAGTTTCTGGCCGTATTGCTGCTCTCAAGGATGCCAAAGGTCAGGTCTGATAACTATAATGTAAACCTTGCAGCAGTTGAGGCCTCCACAAAGTTTAATGCGTGTGAAGACCGATTTGATGAGGACATATCTGATCCATCACCATTAGCAAGTGATAAAAACGATATAGCCACTCTCTAA

Protein Analysis

221

Amino Acids

24.01

Weight (kDa)

5.01

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 19 - 159 7.1e-12 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 176
AclWI GGATC 2 cut(s) 410, 611
AcoI YGGCCR 1 cut(s) 492
AcsI RAATTY 2 cut(s) 104, 395
AcuI CTGAAG 1 cut(s) 266
AfaI GTAC 2 cut(s) 278, 436
AfiI CCNNNNNNNGG 1 cut(s) 157
AhlI ACTAGT 1 cut(s) 437
AluBI AGCT 4 cut(s) 205, 244, 452, 476
AluI AGCT 4 cut(s) 205, 244, 452, 476
Alw21I GWGCWC 1 cut(s) 26
AlwI GGATC 2 cut(s) 410, 611
AoxI GGCC 4 cut(s) 159, 272, 492, 564
ApeKI GCWGC 3 cut(s) 452, 502, 554
ApoI RAATTY 2 cut(s) 104, 395
ArsI GACNNNNNNTTYG 2 cut(s) 512, 544
AsuHPI GGTGA 2 cut(s) 455, 615
AsuII TTCGAA 2 cut(s) 101, 182
BbsI GAAGAC 2 cut(s) 171, 597
Bbv12I GWGCWC 1 cut(s) 26
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 3 cut(s) 439, 489, 566
BccI CCATC 3 cut(s) 427, 464, 628
BceAI ACGGC 2 cut(s) 259, 479
BclI TGATCA 1 cut(s) 129
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 2 cut(s) 438, 462
BisI GCNGC 3 cut(s) 453, 503, 555
BlsI GCNGC 3 cut(s) 454, 504, 556
BmcAI AGTACT 1 cut(s) 436
BmsI GCATC 2 cut(s) 352, 504
BpiI GAAGAC 2 cut(s) 171, 597
BplI GAGNNNNNCTC 2 cut(s) 13, 45
Bpu10I CCTNAGC 1 cut(s) 240
Bpu14I TTCGAA 2 cut(s) 101, 182
BpuEI CTTGAG 3 cut(s) 103, 284, 493
BsaJI CCNNGG 2 cut(s) 75, 269
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 1 cut(s) 157
BseDI CCNNGG 2 cut(s) 75, 269
BseGI GGATG 2 cut(s) 175, 519
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 3 cut(s) 147, 160, 254
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 3 cut(s) 439, 489, 566
BsgI GTGCAG 2 cut(s) 372, 451
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 4 cut(s) 161, 274, 494, 566
BsiHKAI GWGCWC 1 cut(s) 26
BsiSI CCGG 1 cut(s) 122
BslFI GGGAC 2 cut(s) 121, 344
BslI CCNNNNNNNGG 1 cut(s) 157
BsmFI GGGAC 2 cut(s) 121, 344
BsmI GAATGC 2 cut(s) 256, 465
BsnI GGCC 4 cut(s) 161, 274, 494, 566
Bsp119I TTCGAA 2 cut(s) 101, 182
Bsp1286I GDGCHC 1 cut(s) 26
Bsp143I GATC 4 cut(s) 129, 295, 415, 616
BspANI GGCC 4 cut(s) 161, 274, 494, 566
BspCNI CTCAG 3 cut(s) 148, 159, 253
BspFNI CGCG 1 cut(s) 176
BspPI GGATC 2 cut(s) 410, 611
BspT104I TTCGAA 2 cut(s) 101, 182
BssECI CCNNGG 2 cut(s) 75, 269
BssMI GATC 4 cut(s) 129, 295, 415, 616
BssT1I CCWWGG 1 cut(s) 75
Bst4CI ACNGT 1 cut(s) 281
BstBI TTCGAA 2 cut(s) 101, 182
BstC8I GCNNGC 2 cut(s) 203, 385
BstDEI CTNAG 3 cut(s) 146, 156, 240
BstENI CCTNNNNNAGG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 175, 519
BstFNI CGCG 1 cut(s) 176
BstKTI GATC 4 cut(s) 132, 298, 418, 619
BstMBI GATC 4 cut(s) 129, 295, 415, 616
BstMWI GCNNNNNNNGC 1 cut(s) 563
BstNSI RCATGY 1 cut(s) 387
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 3 cut(s) 439, 489, 566
BstV2I GAAGAC 2 cut(s) 171, 597
BsuRI GGCC 4 cut(s) 161, 274, 494, 566
BtgZI GCGATG 1 cut(s) 405
BtsCI GGATG 2 cut(s) 175, 519
BtsIMutI CAGTG 2 cut(s) 153, 286
Cac8I GCNNGC 2 cut(s) 203, 385
CseI GACGC 1 cut(s) 165
Csp6I GTAC 2 cut(s) 277, 435
CviAII CATG 3 cut(s) 10, 380, 384
CviQI GTAC 2 cut(s) 277, 435
DdeI CTNAG 3 cut(s) 146, 156, 240
DpnI GATC 4 cut(s) 131, 297, 417, 618
DpnII GATC 4 cut(s) 129, 295, 415, 616
EaeI YGGCCR 1 cut(s) 492
Eco130I CCWWGG 1 cut(s) 75
Eco147I AGGCCT 1 cut(s) 566
Eco57I CTGAAG 1 cut(s) 266
EcoNI CCTNNNNNAGG 1 cut(s) 155
EcoT14I CCWWGG 1 cut(s) 75
EcoT22I ATGCAT 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 3 cut(s) 13, 383, 387
FaqI GGGAC 2 cut(s) 121, 344
FatI CATG 3 cut(s) 9, 379, 383
FbaI TGATCA 1 cut(s) 129
Fnu4HI GCNGC 3 cut(s) 453, 503, 555
FokI GGATG 2 cut(s) 182, 526
Fsp4HI GCNGC 3 cut(s) 453, 503, 555
FspBI CTAG 2 cut(s) 438, 462
GluI GCNGC 3 cut(s) 453, 503, 555
HaeIII GGCC 4 cut(s) 161, 274, 494, 566
HapII CCGG 1 cut(s) 122
HgaI GACGC 1 cut(s) 165
Hin1II CATG 3 cut(s) 13, 383, 387
HinfI GANTC 1 cut(s) 85
HpaII CCGG 1 cut(s) 122
HphI GGTGA 2 cut(s) 455, 615
Hpy166II GTNNAC 1 cut(s) 547
Hpy188I TCNGA 5 cut(s) 18, 270, 484, 532, 616
Hpy188III TCNNGA 1 cut(s) 82
Hpy8I GTNNAC 1 cut(s) 547
HpyAV CCTTC 2 cut(s) 152, 310
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 6 cut(s) 254, 341, 353, 383, 432, 554
HpyF10VI GCNNNNNNNGC 1 cut(s) 563
HpyF3I CTNAG 3 cut(s) 146, 156, 240
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 3 cut(s) 13, 383, 387
Ksp22I TGATCA 1 cut(s) 129
Kzo9I GATC 4 cut(s) 129, 295, 415, 616
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 9 cut(s) 95, 135, 211, 215, 307, 312, 426, 476, 511
Lsp1109I GCAGC 3 cut(s) 439, 489, 566
LweI GCATC 2 cut(s) 352, 504
MaeI CTAG 2 cut(s) 438, 462
MaeII ACGT 1 cut(s) 349
MalI GATC 4 cut(s) 131, 297, 417, 618
MboI GATC 4 cut(s) 129, 295, 415, 616
MboII GAAGA 3 cut(s) 171, 259, 602
MhlI GDGCHC 1 cut(s) 26
MluCI AATT 4 cut(s) 104, 255, 395, 446
MlyI GAGTC 1 cut(s) 94
MmeI TCCRAC 1 cut(s) 93
Mph1103I ATGCAT 1 cut(s) 385
MseI TTAA 1 cut(s) 579
MspA1I CMGCKG 2 cut(s) 205, 244
MspI CCGG 1 cut(s) 122
Mva1269I GAATGC 2 cut(s) 256, 465
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 563
NdeII GATC 4 cut(s) 129, 295, 415, 616
NlaIII CATG 3 cut(s) 13, 383, 387
NsiI ATGCAT 1 cut(s) 385
NspI RCATGY 1 cut(s) 387
NspV TTCGAA 2 cut(s) 101, 182
PaeI GCATGC 1 cut(s) 387
PceI AGGCCT 1 cut(s) 566
PctI GAATGC 2 cut(s) 256, 465
PkrI GCNGC 3 cut(s) 454, 504, 556
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
PvuII CAGCTG 2 cut(s) 205, 244
RsaI GTAC 2 cut(s) 278, 436
RsaNI GTAC 2 cut(s) 277, 435
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 3 cut(s) 453, 503, 555
Sau3AI GATC 4 cut(s) 129, 295, 415, 616
ScaI AGTACT 1 cut(s) 436
SchI GAGTC 1 cut(s) 94
SduI GDGCHC 1 cut(s) 26
SfaNI GCATC 2 cut(s) 352, 504
SfuI TTCGAA 2 cut(s) 101, 182
SmlI CTYRAG 3 cut(s) 82, 299, 508
SmoI CTYRAG 3 cut(s) 82, 299, 508
SpeI ACTAGT 1 cut(s) 437
SphI GCATGC 1 cut(s) 387
Sse9I AATT 4 cut(s) 104, 255, 395, 446
SseBI AGGCCT 1 cut(s) 566
SspMI CTAG 2 cut(s) 438, 462
StuI AGGCCT 1 cut(s) 566
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 1 cut(s) 352
TaqI TCGA 3 cut(s) 101, 182, 399
TaqII GACCGA 1 cut(s) 609
TasI AATT 4 cut(s) 104, 255, 395, 446
TatI WGTACW 1 cut(s) 434
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 2 cut(s) 160, 286
TseI GCWGC 3 cut(s) 452, 502, 554
TspDTI ATGAA 1 cut(s) 368
TspRI CASTG 2 cut(s) 160, 286
XagI CCTNNNNNAGG 1 cut(s) 155
XapI RAATTY 2 cut(s) 104, 395
XceI RCATGY 1 cut(s) 387
XspI CTAG 2 cut(s) 438, 462
ZrmI AGTACT 1 cut(s) 436
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.