Rh4CG287000

Pectinesterase inhibitor-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005409
Physical Location & Seq
Reverse (-)
54484139 .. 54484818
680 bp
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UTR
Exon/CDS
Intron
Rh4CG287000.1

Sequence Viewer

Length: 549 bp
ATGGTTTCTTCTTCTTCCTCAATAGCTTGCATTGCTGTAATGTCTTTCCTGGTTCTGATGAGTACTACTCCCTCCTCTGCTTTTACTCTAAACAAGGATATTTGCTCCAACACCAACGTCTTGAGTCGCTCTTTCTGTTCGCAATTTCTGGGTTCTAATCCGGTTGTGATCAAGTCCCTCCTTCTCAGCCTCGCCGAGGCCACTATAGATGTTGCATCTTCGAACGCTACGAAGACCAGCCAGCTGATAATCAAGTGGCTAAACCAAACAGACAATCCCCAACTGAAGAATGCAATTAGACAATGTCCGACTCCGTACAATGAGGCCATTGCTGATCTCAAGGAAGCCAAAGAGAAGGTCAGGTCTGGTGACTATGTTGCACTACAGCGTGCAGCAGTTGATGCTTCTATAAAGTTTCATGCATGCGAAGAGAAATTCGAGGACACGCCACCTGATCCATCGCCATTGTGCAGAAATAGTGGTGAATTAGCTGCTATTTCTAGCATCCTTATAGCTGTATTTGAAATTCTGGCAAAATCATACGGGTGA

Protein Analysis

182

Amino Acids

19.57

Weight (kDa)

5.84

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 172 3.6e-13 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 449
AcsI RAATTY 2 cut(s) 434, 525
AcuI CTGAAG 1 cut(s) 305
AfaI GTAC 2 cut(s) 64, 317
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 1 cut(s) 524
AjnI CCWGG 1 cut(s) 48
AluBI AGCT 4 cut(s) 26, 244, 491, 515
AluI AGCT 4 cut(s) 26, 244, 491, 515
AlwI GGATC 1 cut(s) 449
AoxI GGCC 2 cut(s) 198, 324
ApeKI GCWGC 2 cut(s) 392, 491
ApoI RAATTY 2 cut(s) 434, 525
ArsI GACNNNNNNTTYG 2 cut(s) 342, 374
AsuHPI GGTGA 2 cut(s) 380, 494
AsuII TTCGAA 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 239
BbvI GCAGC 2 cut(s) 404, 478
BccI CCATC 1 cut(s) 466
BciT130I CCWGG 1 cut(s) 50
BclI TGATCA 1 cut(s) 168
BfaI CTAG 1 cut(s) 501
BfmI CTRYAG 2 cut(s) 204, 383
BisI GCNGC 2 cut(s) 393, 492
BlsI GCNGC 2 cut(s) 394, 493
BmcAI AGTACT 1 cut(s) 64
Bme1390I CCNGG 1 cut(s) 50
BmrFI CCNGG 1 cut(s) 50
BmsI GCATC 3 cut(s) 224, 391, 513
BpiI GAAGAC 1 cut(s) 239
BplI GAGNNNNNCTC 2 cut(s) 52, 84
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 2 cut(s) 142, 323
BsaJI CCNNGG 1 cut(s) 195
BsaWI WCCGGW 1 cut(s) 160
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse3DI GCAATG 2 cut(s) 30, 327
BseBI CCWGG 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 195
BseGI GGATG 1 cut(s) 504
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMI GCAATG 2 cut(s) 30, 327
BseMII CTCAG 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 64
BseXI GCAGC 2 cut(s) 404, 478
BsgI GTGCAG 2 cut(s) 411, 490
BshFI GGCC 2 cut(s) 200, 326
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 160
BsmI GAATGC 1 cut(s) 295
BsnI GGCC 2 cut(s) 200, 326
Bsp119I TTCGAA 1 cut(s) 221
Bsp143I GATC 3 cut(s) 168, 334, 454
BspANI GGCC 2 cut(s) 200, 326
BspCNI CTCAG 1 cut(s) 198
BspPI GGATC 1 cut(s) 449
BspT104I TTCGAA 1 cut(s) 221
BsrDI GCAATG 2 cut(s) 30, 327
BssECI CCNNGG 1 cut(s) 195
BssMI GATC 3 cut(s) 168, 334, 454
Bst2UI CCWGG 1 cut(s) 50
Bst6I CTCTTC 1 cut(s) 423
BstAPI GCANNNNNTGC 1 cut(s) 401
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 4 cut(s) 28, 242, 390, 424
BstDEI CTNAG 1 cut(s) 185
BstENI CCTNNNNNAGG 1 cut(s) 194
BstF5I GGATG 1 cut(s) 504
BstKTI GATC 3 cut(s) 171, 337, 457
BstMBI GATC 3 cut(s) 168, 334, 454
BstMWI GCNNNNNNNGC 2 cut(s) 32, 401
BstNI CCWGG 1 cut(s) 50
BstNSI RCATGY 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 48
BstSFI CTRYAG 2 cut(s) 204, 383
BstV1I GCAGC 2 cut(s) 404, 478
BstV2I GAAGAC 1 cut(s) 239
BsuRI GGCC 2 cut(s) 200, 326
BtgZI GCGATG 1 cut(s) 444
BtsCI GGATG 1 cut(s) 504
Cac8I GCNNGC 4 cut(s) 28, 242, 390, 424
Csp6I GTAC 2 cut(s) 63, 316
CviAII CATG 2 cut(s) 419, 423
CviQI GTAC 2 cut(s) 63, 316
DdeI CTNAG 1 cut(s) 185
DpnI GATC 3 cut(s) 170, 336, 456
DpnII GATC 3 cut(s) 168, 334, 454
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
Eco57I CTGAAG 1 cut(s) 305
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 424
FaeI CATG 2 cut(s) 422, 426
FaiI YATR 7 cut(s) 206, 375, 410, 420, 424, 512, 541
FaqI GGGAC 1 cut(s) 160
FatI CATG 2 cut(s) 418, 422
FbaI TGATCA 1 cut(s) 168
Fnu4HI GCNGC 2 cut(s) 393, 492
FokI GGATG 1 cut(s) 491
Fsp4HI GCNGC 2 cut(s) 393, 492
FspBI CTAG 1 cut(s) 501
GluI GCNGC 2 cut(s) 393, 492
HaeIII GGCC 2 cut(s) 200, 326
HapII CCGG 1 cut(s) 161
Hin1II CATG 2 cut(s) 422, 426
HinfI GANTC 2 cut(s) 124, 310
HpaII CCGG 1 cut(s) 161
HphI GGTGA 2 cut(s) 380, 494
Hpy188I TCNGA 2 cut(s) 57, 309
Hpy188III TCNNGA 1 cut(s) 121
HpyAV CCTTC 2 cut(s) 191, 349
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 7 cut(s) 30, 215, 293, 380, 392, 422, 471
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 401
HpyF3I CTNAG 1 cut(s) 185
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 2 cut(s) 422, 426
Ksp22I TGATCA 1 cut(s) 168
Kzo9I GATC 3 cut(s) 168, 334, 454
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 2 cut(s) 404, 478
LweI GCATC 3 cut(s) 224, 391, 513
MaeI CTAG 1 cut(s) 501
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 368
MalI GATC 3 cut(s) 170, 336, 456
MboI GATC 3 cut(s) 168, 334, 454
MboII GAAGA 6 cut(s) 3, 6, 210, 244, 298, 440
MluCI AATT 5 cut(s) 143, 294, 434, 485, 525
MlyI GAGTC 2 cut(s) 133, 304
MmeI TCCRAC 2 cut(s) 132, 332
MnlI CCTC 8 cut(s) 28, 82, 85, 188, 190, 200, 316, 433
Mph1103I ATGCAT 1 cut(s) 424
MslI CAYNNNNRTG 1 cut(s) 544
MspA1I CMGCKG 1 cut(s) 244
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 50
Mva1269I GAATGC 1 cut(s) 295
MvaI CCWGG 1 cut(s) 50
MwoI GCNNNNNNNGC 2 cut(s) 32, 401
NdeII GATC 3 cut(s) 168, 334, 454
NlaIII CATG 2 cut(s) 422, 426
NmeAIII GCCGAG 1 cut(s) 220
NmuCI GTSAC 1 cut(s) 368
NsiI ATGCAT 1 cut(s) 424
NspI RCATGY 1 cut(s) 426
NspV TTCGAA 1 cut(s) 221
PaeI GCATGC 1 cut(s) 426
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PctI GAATGC 1 cut(s) 295
PflFI GACNNNGTC 1 cut(s) 303
PkrI GCNGC 2 cut(s) 394, 493
PleI GAGTC 2 cut(s) 132, 304
PpsI GAGTC 2 cut(s) 132, 304
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PsyI GACNNNGTC 1 cut(s) 303
PvuII CAGCTG 1 cut(s) 244
RsaI GTAC 2 cut(s) 64, 317
RsaNI GTAC 2 cut(s) 63, 316
RseI CAYNNNNRTG 1 cut(s) 544
SatI GCNGC 2 cut(s) 393, 492
Sau3AI GATC 3 cut(s) 168, 334, 454
ScaI AGTACT 1 cut(s) 64
SchI GAGTC 2 cut(s) 133, 304
ScrFI CCNGG 1 cut(s) 50
SetI ASST 8 cut(s) 28, 120, 246, 360, 365, 454, 493, 517
SfaNI GCATC 3 cut(s) 224, 391, 513
SfcI CTRYAG 2 cut(s) 204, 383
SfuI TTCGAA 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 544
SmlI CTYRAG 2 cut(s) 121, 338
SmoI CTYRAG 2 cut(s) 121, 338
SphI GCATGC 1 cut(s) 426
Sse9I AATT 5 cut(s) 143, 294, 434, 485, 525
SspMI CTAG 1 cut(s) 501
StyD4I CCNGG 1 cut(s) 48
TaiI ACGT 1 cut(s) 120
TaqI TCGA 2 cut(s) 221, 438
TasI AATT 5 cut(s) 143, 294, 434, 485, 525
TatI WGTACW 1 cut(s) 62
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 2 cut(s) 392, 491
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 1 cut(s) 407
TspGWI ACGGA 1 cut(s) 303
Tth111I GACNNNGTC 1 cut(s) 303
XagI CCTNNNNNAGG 1 cut(s) 194
XapI RAATTY 2 cut(s) 434, 525
XceI RCATGY 1 cut(s) 426
XspI CTAG 1 cut(s) 501
ZrmI AGTACT 1 cut(s) 64
Zsp2I ATGCAT 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.