Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0005383

Physical Location & Seq

Genomic Coordinates Reverse (-)

10990232 .. 10995924

Gene Structure 5693 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGGCTGTTCAGACTTCTACTAAACTGAATTTATGTGAAGACGAGGATGATGAACCTTTAGTATTCAAACGGTACAGCACAAAATCTAAACCGAATCAATTAAACGCTGAAGCAAAGAAATTATCAGCTGAGAGGCAAGTTGGACAACCAGGGAAGGAGGTATCTGAGCAACGTTCTTCCAATGGTCTAAACTCGAGTGTTCAAAAGGGTAAGATGGTTCCATCAACCAAATCACCACCAGTAAAATCTCCGCTAGCAAGCCCAAAAGCTTCAGCTTCATCTGCTGGGGCATCACCAGTATCAAATTCAAAAGCTTCAACTTCATCTAGGGCATCACCAGTATCCAAATCACCACCGTTGACTACATTAAATGGTCAGTCAAAGCGGTTGTCAGAACAGAATAAGTCTACCACAATTAAGGAGGAAATTAATTCTATTGAACATCTTGCTCAAGGAAATAGTGATTCTGAAGATGAAAAACCGATAGGTGCCAGACTGGGGAAAGTGAAACCTTCTGAAGATTCGGATGATGATAAACCTTTATCATCAAGGATTGCACCGAGGTCCAAGTTGGGAACATCAGGCAGTCGGCCTTATGATTCTAATGAAAGGAAGCCCATTGTTTCAAAAGTTCAGAAGAATGGTTCCAGCACAATGGATAAAGGCAAAGCTTCATCTGTGGTCTCTAGTAAGAGGCCTCTAGATAAGGTCAATTCTTCTGAGAACTGTTCAGCTAAAAGGCCAAAAGTTTCAGATTCTTCTACATCTGCAAAGAATAAGCAGGTATCAGCCAAACAAGAACCAAAGGAAGAAGATGATGATGATGATGATGACTTCATTCCAATTTCCCAGAGGAGTAAGAAAGTTGCATCGGATAGTAAACCATCTCTGACAAAGCTAAAGGTAACCAAAGTTGGTTCATCCTCAATCAAGAAAACGATCAAGAAGGGCCAAAAAGCTTCAAAAATTTCAAAGTATTCTAAGTCGACAACAAAGCCACCGGGCTCTAACGATGGGCAGATAAAATGGACTACTTTAAAACACAATGGTGTCATTTTCCCGCCTCCATACAACCCTCATGGGGTAAAGATGCTCTATAATGGGAAGCCAGTCACCTTGACTCCTGAACAAGAGGAGGTTGCAACAATGTATGCAGTGATGAAAGATACTGATTATGTACAAAAAGAAACATTCAGAAACAATTTCTGGGCTGATTGGCATAAGTTTCTTGGGAAGAACCATGTAATTCAGAAATTGGATGCTTGTGATTTTACGCCAATATATGACTGGTATCAGAATGAAAAGGAAAAGAAGAAACAAATGAGCACGGAAGAGAAGAAGGCCTTGAAGGAGGAGAAACTGAAACAAGAGGAGAAGTATATGTGGGCCGAGGTTGATGGTGATAAGGAGAAGGTTGGAAACTTCAGAGTTGAACCACCTGGGTTATTTCGAGGCCGTGGAGAGCATCCAAAGTCGGGAAAGCTGAAAAGACGGATTCGTCCAAGTGATGTTACAATTAATATAGGAAAGGATGCCCCAATTCCAGAATGTCCCATCCCTGGTGAAAGCTGGAAAGAAGTAAGACATGACAATACAGTTACATGGTTAGCTTTCTGGAATGATCCAATTAATCAAAAGGAATTCAAATACGTATTTCTGGCACCTAGTAGTTCCTTGAAGGGGAAAAGTGACGAGCTGAAATATGATAAAGCAAGGAGATTGACAGACTGCATAGGAAAAATCAGGGCTTCATACACCAAGGATTTTACTAGTAAAGATGTGTCTAAGCGGCAGATTGCGGTTGCTACCTATCTCATTGATAAACTTGCCCTCAGGGCGGGCAATGAGAAGGATGATGATGAAGCCGATACTGTTGGTTGCTGCACTTTAAAAGTAGAGAATGTAAAAGCAATCCCCCCTAACTCGTTGGAGTTCAACTTCCTTGGTAAAGATTCAATCAGATATGAAAATACTGTTGAAGTTGAGATTCCTGTTTACAAGGCAATTATGCAGTTCCAGTCTGGGAAAAGTGGCAGTGATGATCTCTTTGACTTGTTGGATACCAGTAGATTGAATGCTCATCTAAAGGAAAAAATGGATGGCCTTACAGCGAAAGTGTTTCGTACCTACAATGCATCTATCACATTGGATAACATATTGAACAAGGAAACCAAAGACGGAGATCTTTCAGAGAAACTCGTTGTTTATCAGCATGCAAACAAAGAGGTTGCCATTATTTGTAATCATCAGCGTACCGTCTCAAAGTCTCACAGTGCACAAATGTCTAAGTTAACTGAGAAGATTGCTGAACTTCAGGGCAACCTGAAAGAGCTGAAAGTAGATTTGGACAGGGCCAAAAAAGGAAAGCCCCCACTGAAAGATGCTGATGGAAAACAAAAGAAAAATTTGACCCCGGAAGCTTTGGAGAGGAAGATAGCTCAAACAAATGCAAAGATAGAAAAAATGGAACTGGATATGAGAATTAAAGAGGATCTGAAAACAGTGGCATTGGGAACGTCGAAAATCAACTATCTTGACGAGAGGATCACCGTTGCTTGGTGCAAGCGACATGAAGTTCCCATTGAGAAGATATTCAACAAGTCGCTCCTCGCAAAGTTTTCTTGGGCAATGCGAGTGGAGCCTGACTTCAGATTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

886

Amino Acids

98.93

Weight (kDa)

9.39

Isoelectric Point (pI)

38.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Topoisom_I_N	PF02919	341 - 553	1.2e-94	Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I	PF01028	556 - 780	6.2e-93	Eukaryotic DNA topoisomerase I, catalytic core
Topo_C_assoc	PF14370	817 - 885	2.2e-33	C-terminal topoisomerase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005383)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55310
fragaria_vesca	FvH4_4g16730 FvH4_4g16730
malus_domestica	MD00G1109500.v1.1 MD13G1176900.v1.1 MD16G1178600.v1.1
prunus_persica	Prupe.1G146000_v2.0.a1 Prupe.1G146000_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1 Prupe.8G003200_v2.0.a1
pyrus_communis	pycom10g21080 pycom13g15260 pycom16g15010
rosa_chinensis	RchiOBHm_Chr4g0420301
rosa_laevigata	RLG00000003998 RLG00000007777 RLG00000007780
rosa_multiflora	Rmu_sc0001767.1_g000005
rosa_roxburghii	Rroxscaffold_5G00362940
rosa_rugosa	Rorug04G0163000 Rorug04G0163100 Rorug04G0163200
rosa_samantha	Rh4AG224100 Rh4BG227100 Rh4CG238200
rosa_wichuraiana	Rw4G019300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1497
Acc36I	ACCTGC	1 cut(s)	772
AccB1I	GGYRCC	2 cut(s)	488, 1660
AccI	GTMKAC	2 cut(s)	407, 986
AciI	CCGC	6 cut(s)	251, 385, 1061, 1789, 1799, 1838
AclI	AACGTT	1 cut(s)	172
AclWI	GGATC	3 cut(s)	1616, 2499, 2552
AcsI	RAATTY	5 cut(s)	28, 304, 966, 1640, 2404
AcuI	CTGAAG	7 cut(s)	129, 255, 489, 537, 1408, 2297, 2632
AfaI	GTAC	4 cut(s)	74, 1179, 2125, 2254
AfiI	CCNNNNNNNGG	7 cut(s)	498, 1081, 1475, 1559, 1680, 1837, 2556
AhlI	ACTAGT	1 cut(s)	1769
AjnI	CCWGG	3 cut(s)	148, 1438, 1558
AjuI	GAANNNNNNNTTGG	2 cut(s)	2327, 2359
AleI	CACNNNNGTG	1 cut(s)	1047
Alw21I	GWGCWC	2 cut(s)	1328, 2278
Alw26I	GTCTC	3 cut(s)	688, 2263, 2271
Alw44I	GTGCAC	1 cut(s)	2274
AlwI	GGATC	3 cut(s)	1616, 2499, 2552
AlwNI	CAGNNNCTG	1 cut(s)	2655
Ama87I	CYCGRG	1 cut(s)	193
AoxI	GGCC	9 cut(s)	590, 695, 740, 949, 1341, 1386, 1453, 2101, 2352
ApaLI	GTGCAC	1 cut(s)	2274
ApeKI	GCWGC	1 cut(s)	1881
ApoI	RAATTY	5 cut(s)	28, 304, 966, 1640, 2404
ArsI	GACNNNNNNTTYG	2 cut(s)	374, 406
AseI	ATTAAT	3 cut(s)	429, 1518, 1629
Asp700I	GAANNNNTTC	1 cut(s)	2591
AspS9I	GGNCC	4 cut(s)	564, 949, 1386, 2352
AsuC2I	CCSGG	2 cut(s)	1002, 2414
AsuHPI	GGTGA	8 cut(s)	225, 285, 327, 342, 1105, 1412, 1574, 2539
AsuNHI	GCTAGC	1 cut(s)	253
AvaI	CYCGRG	1 cut(s)	193
AvaII	GGWCC	1 cut(s)	564
AxyI	CCTNAGG	1 cut(s)	1832
BaeGI	GKGCMC	1 cut(s)	2278
BanI	GGYRCC	2 cut(s)	488, 1660
BanII	GRGCYC	1 cut(s)	1007
BbsI	GAAGAC	1 cut(s)	45
Bbv12I	GWGCWC	2 cut(s)	1328, 2278
BbvI	GCAGC	1 cut(s)	1868
BccI	CCATC	8 cut(s)	208, 229, 892, 1007, 1391, 1562, 2093, 2381
BceAI	ACGGC	1 cut(s)	1440
BciT130I	CCWGG	3 cut(s)	150, 1440, 1560
BciVI	GTATCC	2 cut(s)	352, 2053
BcnI	CCSGG	2 cut(s)	1002, 2414
BcoDI	GTCTC	3 cut(s)	688, 2263, 2271
BcuI	ACTAGT	1 cut(s)	1769
BfaI	CTAG	6 cut(s)	254, 327, 687, 701, 1665, 1770
BfuAI	ACCTGC	1 cut(s)	772
BfuI	GTATCC	2 cut(s)	352, 2053
BglI	GCCNNNNNGGC	1 cut(s)	1835
BglII	AGATCT	1 cut(s)	2182
BisI	GCNGC	2 cut(s)	1790, 1882
BlsI	GCNGC	2 cut(s)	1791, 1883
Bme1390I	CCNGG	5 cut(s)	150, 1002, 1440, 1560, 2414
Bme18I	GGWCC	1 cut(s)	564
BmeT110I	CYCGRG	1 cut(s)	193
BmgT120I	GGNCC	4 cut(s)	564, 949, 1386, 2352
BmiI	GGNNCC	5 cut(s)	219, 490, 646, 1662, 2640
BmrFI	CCNGG	5 cut(s)	150, 1002, 1440, 1560, 2414
BmrI	ACTGGG	1 cut(s)	506
BmsI	GCATC	9 cut(s)	299, 341, 878, 1080, 1249, 1474, 1522, 2144, 2371
BmtI	GCTAGC	1 cut(s)	257
BmuI	ACTGGG	1 cut(s)	506
BpiI	GAAGAC	1 cut(s)	45
BpuEI	CTTGAG	1 cut(s)	435
BpuMI	CCSGG	2 cut(s)	1002, 2414
BsaAI	YACGTR	1 cut(s)	1651
BsaI	GGTCTC	1 cut(s)	688
BsaJI	CCNNGG	9 cut(s)	149, 560, 1389, 1439, 1456, 1558, 1758, 1942, 2412
BsaXI	ACNNNNNCTCC	2 cut(s)	1105, 1135
Bsc4I	CCNNNNNNNGG	7 cut(s)	498, 1081, 1475, 1559, 1680, 1837, 2556
Bse1I	ACTGG	9 cut(s)	239, 296, 338, 501, 1109, 1292, 2017, 2064, 2475
Bse21I	CCTNAGG	1 cut(s)	1832
Bse3DI	GCAATG	2 cut(s)	1849, 2634
BseBI	CCWGG	3 cut(s)	150, 1440, 1560
BseDI	CCNNGG	9 cut(s)	149, 560, 1389, 1439, 1456, 1558, 1758, 1942, 2412
BseGI	GGATG	9 cut(s)	52, 532, 920, 1264, 1465, 1537, 1554, 1858, 2104
BseLI	CCNNNNNNNGG	7 cut(s)	498, 1081, 1475, 1559, 1680, 1837, 2556
BseMI	GCAATG	2 cut(s)	1849, 2634
BseMII	CTCAG	5 cut(s)	120, 156, 711, 1846, 2286
BseNI	ACTGG	9 cut(s)	239, 296, 338, 501, 1109, 1292, 2017, 2064, 2475
BseRI	GAGGAG	5 cut(s)	868, 1148, 1367, 1385, 2597
BseSI	GKGCMC	1 cut(s)	2278
BseXI	GCAGC	1 cut(s)	1868
BseYI	CCCAGC	1 cut(s)	284
BsgI	GTGCAG	1 cut(s)	1867
BshFI	GGCC	9 cut(s)	592, 697, 742, 951, 1343, 1388, 1455, 2103, 2354
BshNI	GGYRCC	2 cut(s)	488, 1660
BsiHKAI	GWGCWC	2 cut(s)	1328, 2278
BsiHKCI	CYCGRG	1 cut(s)	193
BsiSI	CCGG	2 cut(s)	1001, 2414
BslFI	GGGAC	1 cut(s)	1536
BslI	CCNNNNNNNGG	7 cut(s)	498, 1081, 1475, 1559, 1680, 1837, 2556
BsmAI	GTCTC	3 cut(s)	688, 2263, 2271
BsmBI	CGTCTC	1 cut(s)	2263
BsmFI	GGGAC	1 cut(s)	1536
BsmI	GAATGC	1 cut(s)	2080
BsnI	GGCC	9 cut(s)	592, 697, 742, 951, 1343, 1388, 1455, 2103, 2354
Bso31I	GGTCTC	1 cut(s)	688
BsoBI	CYCGRG	1 cut(s)	193
Bsp1286I	GDGCHC	3 cut(s)	1007, 1328, 2278
Bsp1407I	TGTACA	1 cut(s)	1177
Bsp143I	GATC	6 cut(s)	939, 1621, 2041, 2182, 2491, 2544
BspACI	CCGC	6 cut(s)	251, 385, 1061, 1789, 1799, 1838
BspANI	GGCC	9 cut(s)	592, 697, 742, 951, 1343, 1388, 1455, 2103, 2354
BspCNI	CTCAG	5 cut(s)	121, 157, 712, 1845, 2287
BspLI	GGNNCC	5 cut(s)	219, 490, 646, 1662, 2640
BspMI	ACCTGC	1 cut(s)	772
BspOI	GCTAGC	1 cut(s)	257
BspPI	GGATC	3 cut(s)	1616, 2499, 2552
BspT107I	GGYRCC	2 cut(s)	488, 1660
BspTNI	GGTCTC	1 cut(s)	688
BsrDI	GCAATG	2 cut(s)	1849, 2634
BsrGI	TGTACA	1 cut(s)	1177
BsrI	ACTGG	9 cut(s)	239, 296, 338, 501, 1109, 1292, 2017, 2064, 2475
BssECI	CCNNGG	9 cut(s)	149, 560, 1389, 1439, 1456, 1558, 1758, 1942, 2412
BssMI	GATC	6 cut(s)	939, 1621, 2041, 2182, 2491, 2544
BssT1I	CCWWGG	2 cut(s)	1758, 1942
Bst2UI	CCWGG	3 cut(s)	150, 1440, 1560
Bst6I	CTCTTC	1 cut(s)	1326
BstAUI	TGTACA	1 cut(s)	1177
BstBAI	YACGTR	1 cut(s)	1651
BstC8I	GCNNGC	5 cut(s)	255, 259, 1840, 2214, 2564
BstDEI	CTNAG	8 cut(s)	129, 165, 720, 981, 1785, 1832, 2286, 2295
BstDSI	CCRYGG	1 cut(s)	1456
BstEII	GGTNACC	1 cut(s)	904
BstF5I	GGATG	9 cut(s)	52, 532, 920, 1264, 1465, 1537, 1554, 1858, 2104
BstKTI	GATC	6 cut(s)	942, 1624, 2044, 2185, 2494, 2547
BstMAI	GTCTC	3 cut(s)	688, 2263, 2271
BstMBI	GATC	6 cut(s)	939, 1621, 2041, 2182, 2491, 2544
BstMWI	GCNNNNNNNGC	3 cut(s)	281, 1835, 2638
BstNI	CCWGG	3 cut(s)	150, 1440, 1560
BstNSI	RCATGY	1 cut(s)	2216
BstPI	GGTNACC	1 cut(s)	904
BstSCI	CCNGG	5 cut(s)	148, 1000, 1438, 1558, 2412
BstSLI	GKGCMC	1 cut(s)	2278
BstSNI	TACGTA	1 cut(s)	1651
BstV1I	GCAGC	1 cut(s)	1868
BstV2I	GAAGAC	1 cut(s)	45
BstX2I	RGATCY	2 cut(s)	2182, 2491
BstXI	CCANNNNNNTGG	1 cut(s)	655
BstYI	RGATCY	2 cut(s)	2182, 2491
Bsu36I	CCTNAGG	1 cut(s)	1832
BsuI	GTATCC	2 cut(s)	352, 2053
BsuRI	GGCC	9 cut(s)	592, 697, 742, 951, 1343, 1388, 1455, 2103, 2354
BtgI	CCRYGG	1 cut(s)	1456
BtsCI	GGATG	9 cut(s)	52, 532, 920, 1264, 1465, 1537, 1554, 1858, 2104
BtsI	GCAGTG	2 cut(s)	1161, 2041
BtsIMutI	CAGTG	5 cut(s)	1161, 2041, 2278, 2372, 2508
BveI	ACCTGC	1 cut(s)	772
Cac8I	GCNNGC	5 cut(s)	255, 259, 1840, 2214, 2564
CaiI	CAGNNNCTG	1 cut(s)	2655
Cfr13I	GGNCC	4 cut(s)	564, 949, 1386, 2352
Csp6I	GTAC	4 cut(s)	73, 1178, 2124, 2253
CspCI	CAANNNNNGTGG	2 cut(s)	2616, 2651
CviAII	CATG	6 cut(s)	1079, 1241, 1586, 1602, 2213, 2570
CviQI	GTAC	4 cut(s)	73, 1178, 2124, 2253
DdeI	CTNAG	8 cut(s)	129, 165, 720, 981, 1785, 1832, 2286, 2295
DpnI	GATC	6 cut(s)	941, 1623, 2043, 2184, 2493, 2546
DpnII	GATC	6 cut(s)	939, 1621, 2041, 2182, 2491, 2544
DraI	TTTAAA	2 cut(s)	1038, 1890
DrdI	GACNNNNNNGTC	1 cut(s)	1497
DseDI	GACNNNNNNGTC	1 cut(s)	1497
Eam1104I	CTCTTC	1 cut(s)	1326
EarI	CTCTTC	1 cut(s)	1326
Eco105I	TACGTA	1 cut(s)	1651
Eco130I	CCWWGG	2 cut(s)	1758, 1942
Eco147I	AGGCCT	2 cut(s)	697, 1343
Eco24I	GRGCYC	1 cut(s)	1007
Eco31I	GGTCTC	1 cut(s)	688
Eco47I	GGWCC	1 cut(s)	564
Eco57I	CTGAAG	7 cut(s)	129, 255, 489, 537, 1408, 2297, 2632
Eco81I	CCTNAGG	1 cut(s)	1832
Eco88I	CYCGRG	1 cut(s)	193
Eco91I	GGTNACC	1 cut(s)	904
EcoO65I	GGTNACC	1 cut(s)	904
EcoRI	GAATTC	1 cut(s)	1640
EcoRII	CCWGG	3 cut(s)	148, 1438, 1558
EcoT14I	CCWWGG	2 cut(s)	1758, 1942
EcoT22I	ATGCAT	1 cut(s)	2137
EcoT38I	GRGCYC	1 cut(s)	1007
ErhI	CCWWGG	2 cut(s)	1758, 1942
Esp3I	CGTCTC	1 cut(s)	2263
FaeI	CATG	6 cut(s)	1082, 1244, 1589, 1605, 2216, 2573
FalI	AAGNNNNNCTT	2 cut(s)	1328, 1360
FaqI	GGGAC	1 cut(s)	1536
FatI	CATG	6 cut(s)	1078, 1240, 1585, 1601, 2212, 2569
FauI	CCCGC	2 cut(s)	1068, 1831
FblI	GTMKAC	2 cut(s)	407, 986
Fnu4HI	GCNGC	2 cut(s)	1790, 1882
FokI	GGATG	9 cut(s)	59, 539, 907, 1271, 1452, 1541, 1544, 1865, 2111
FriOI	GRGCYC	1 cut(s)	1007
Fsp4HI	GCNGC	2 cut(s)	1790, 1882
FspBI	CTAG	6 cut(s)	254, 327, 687, 701, 1665, 1770
GluI	GCNGC	2 cut(s)	1790, 1882
GsaI	CCCAGC	1 cut(s)	288
HaeIII	GGCC	9 cut(s)	592, 697, 742, 951, 1343, 1388, 1455, 2103, 2354
HapII	CCGG	2 cut(s)	1001, 2414
Hin1II	CATG	6 cut(s)	1082, 1244, 1589, 1605, 2216, 2573
HincII	GTYRAC	3 cut(s)	360, 987, 2292
HindII	GTYRAC	3 cut(s)	360, 987, 2292
HindIII	AAGCTT	5 cut(s)	267, 312, 669, 957, 2418
HpaI	GTTAAC	1 cut(s)	2292
HpaII	CCGG	2 cut(s)	1001, 2414
HphI	GGTGA	8 cut(s)	225, 285, 327, 342, 1105, 1412, 1574, 2539
Hpy166II	GTNNAC	7 cut(s)	360, 408, 881, 987, 1996, 2276, 2292
Hpy188III	TCNNGA	8 cut(s)	701, 931, 943, 1124, 1476, 1544, 1615, 2534
Hpy8I	GTNNAC	7 cut(s)	360, 408, 881, 987, 1996, 2276, 2292
Hpy99I	CGWCG	1 cut(s)	2521
HpyAV	CCTTC	8 cut(s)	148, 522, 940, 1333, 1342, 1405, 1672, 1843
HpyCH4IV	ACGT	3 cut(s)	172, 1650, 2516
HpyF10VI	GCNNNNNNNGC	3 cut(s)	281, 1835, 2638
HpyF3I	CTNAG	8 cut(s)	129, 165, 720, 981, 1785, 1832, 2286, 2295
HpySE526I	ACGT	3 cut(s)	172, 1650, 2516
Hsp92II	CATG	6 cut(s)	1082, 1244, 1589, 1605, 2216, 2573
KspAI	GTTAAC	1 cut(s)	2292
Kzo9I	GATC	6 cut(s)	939, 1621, 2041, 2182, 2491, 2544
LmnI	GCTCC	2 cut(s)	2610, 2638
Lsp1109I	GCAGC	1 cut(s)	1868
LweI	GCATC	9 cut(s)	299, 341, 878, 1080, 1249, 1474, 1522, 2144, 2371
MaeI	CTAG	6 cut(s)	254, 327, 687, 701, 1665, 1770
MaeII	ACGT	3 cut(s)	172, 1650, 2516
MaeIII	GTNAC	5 cut(s)	904, 1111, 1510, 1597, 1688
MalI	GATC	6 cut(s)	941, 1623, 2043, 2184, 2493, 2546
MboI	GATC	6 cut(s)	939, 1621, 2041, 2182, 2491, 2544
MflI	RGATCY	2 cut(s)	2182, 2491
MhlI	GDGCHC	3 cut(s)	1007, 1328, 2278
MlyI	GAGTC	1 cut(s)	1114
MmeI	TCCRAC	4 cut(s)	121, 1396, 1908, 2037
Mph1103I	ATGCAT	1 cut(s)	2137
MroXI	GAANNNNTTC	1 cut(s)	2591
MslI	CAYNNNNRTG	1 cut(s)	1047
MspA1I	CMGCKG	1 cut(s)	128
MspI	CCGG	2 cut(s)	1001, 2414
MspR9I	CCNGG	5 cut(s)	150, 1002, 1440, 1560, 2414
Mva1269I	GAATGC	1 cut(s)	2080
MvaI	CCWGG	3 cut(s)	150, 1440, 1560
MwoI	GCNNNNNNNGC	3 cut(s)	281, 1835, 2638
NciI	CCSGG	2 cut(s)	1002, 2414
NdeII	GATC	6 cut(s)	939, 1621, 2041, 2182, 2491, 2544
NheI	GCTAGC	1 cut(s)	253
NlaIII	CATG	6 cut(s)	1082, 1244, 1589, 1605, 2216, 2573
NlaIV	GGNNCC	5 cut(s)	219, 490, 646, 1662, 2640
NmeAIII	GCCGAG	1 cut(s)	1414
NmuCI	GTSAC	2 cut(s)	1111, 1688
NsiI	ATGCAT	1 cut(s)	2137
NspI	RCATGY	1 cut(s)	2216
OliI	CACNNNNGTG	1 cut(s)	1047
PaeI	GCATGC	1 cut(s)	2216
PaeR7I	CTCGAG	1 cut(s)	193
PceI	AGGCCT	2 cut(s)	697, 1343
PctI	GAATGC	1 cut(s)	2080
PdmI	GAANNNNTTC	1 cut(s)	2591
PfeI	GAWTC	9 cut(s)	94, 464, 521, 599, 755, 1495, 1952, 1987, 2652
PkrI	GCNGC	2 cut(s)	1791, 1883
PleI	GAGTC	1 cut(s)	1114
PpsI	GAGTC	1 cut(s)	1114
Ppu21I	YACGTR	1 cut(s)	1651
PshBI	ATTAAT	3 cut(s)	429, 1518, 1629
Psp1406I	AACGTT	1 cut(s)	172
Psp6I	CCWGG	3 cut(s)	148, 1438, 1558
PspEI	GGTNACC	1 cut(s)	904
PspFI	CCCAGC	1 cut(s)	284
PspGI	CCWGG	3 cut(s)	148, 1438, 1558
PspN4I	GGNNCC	5 cut(s)	219, 490, 646, 1662, 2640
PspPI	GGNCC	4 cut(s)	564, 949, 1386, 2352
PspXI	VCTCGAGB	1 cut(s)	193
PstNI	CAGNNNCTG	1 cut(s)	2655
PsuI	RGATCY	2 cut(s)	2182, 2491
PvuII	CAGCTG	1 cut(s)	128
RsaI	GTAC	4 cut(s)	74, 1179, 2125, 2254
RsaNI	GTAC	4 cut(s)	73, 1178, 2124, 2253
RseI	CAYNNNNRTG	1 cut(s)	1047
SalI	GTCGAC	1 cut(s)	985
SatI	GCNGC	2 cut(s)	1790, 1882
Sau3AI	GATC	6 cut(s)	939, 1621, 2041, 2182, 2491, 2544
Sau96I	GGNCC	4 cut(s)	564, 949, 1386, 2352
SchI	GAGTC	1 cut(s)	1114
ScrFI	CCNGG	5 cut(s)	150, 1002, 1440, 1560, 2414
SduI	GDGCHC	3 cut(s)	1007, 1328, 2278
SfaNI	GCATC	9 cut(s)	299, 341, 878, 1080, 1249, 1474, 1522, 2144, 2371
Sfr274I	CTCGAG	1 cut(s)	193
SinI	GGWCC	1 cut(s)	564
SlaI	CTCGAG	1 cut(s)	193
SmiMI	CAYNNNNRTG	1 cut(s)	1047
SmlI	CTYRAG	2 cut(s)	193, 450
SmoI	CTYRAG	2 cut(s)	193, 450
SnaBI	TACGTA	1 cut(s)	1651
SpeI	ACTAGT	1 cut(s)	1769
SphI	GCATGC	1 cut(s)	2216
SseBI	AGGCCT	2 cut(s)	697, 1343
SsiI	CCGC	6 cut(s)	251, 385, 1061, 1789, 1799, 1838
SspMI	CTAG	6 cut(s)	254, 327, 687, 701, 1665, 1770
StuI	AGGCCT	2 cut(s)	697, 1343
StyD4I	CCNGG	5 cut(s)	148, 1000, 1438, 1558, 2412
StyI	CCWWGG	2 cut(s)	1758, 1942
TaiI	ACGT	3 cut(s)	175, 1653, 2519
TaqI	TCGA	4 cut(s)	194, 986, 1450, 2519
TatI	WGTACW	1 cut(s)	1177
TauI	GCSGC	1 cut(s)	1792
TfiI	GAWTC	9 cut(s)	94, 464, 521, 599, 755, 1495, 1952, 1987, 2652
TscAI	CASTG	5 cut(s)	1161, 2041, 2278, 2379, 2508
TseFI	GTSAC	2 cut(s)	1111, 1688
TseI	GCWGC	1 cut(s)	1881
Tsp45I	GTSAC	2 cut(s)	1111, 1688
TspGWI	ACGGA	3 cut(s)	1343, 1507, 2193
TspRI	CASTG	5 cut(s)	1161, 2041, 2278, 2379, 2508
VneI	GTGCAC	1 cut(s)	2274
VpaK11BI	GGWCC	1 cut(s)	564
VspI	ATTAAT	3 cut(s)	429, 1518, 1629
XapI	RAATTY	5 cut(s)	28, 304, 966, 1640, 2404
XbaI	TCTAGA	1 cut(s)	700
XceI	RCATGY	1 cut(s)	2216
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1284
XhoI	CTCGAG	1 cut(s)	193
XmiI	GTMKAC	2 cut(s)	407, 986
XmnI	GAANNNNTTC	1 cut(s)	2591
XspI	CTAG	6 cut(s)	254, 327, 687, 701, 1665, 1770
Zsp2I	ATGCAT	1 cut(s)	2137

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.