pycom16g22060

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007226
Physical Location & Seq
Reverse (-)
21554831 .. 21555214
384 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g22060.1

Sequence Viewer

Length: 384 bp
ATGCACATGGACAAGTGGTCTTTCTTGAGCCGCCTAAGGAAGGTTGTGAGGAAGGTAAGCTTCCTACTAAACCTCAACGTGAATCGCTGGCGAATTGTTTCGATGATCGGCCGTACTGTTTCTAGCAACCAGCAATTGATGAGCTTCAACGGCCACCCAGGGCTGAAAGCGGCGTGTGGCGATGAGACCAGCTCCGAGGAGGACTCGGGGTCTTCTAGGGGACATCTTCAGAGGACAATAAGCTATCCATCGGATCGAGATGAAATGGATCAAAGAGCAGAGATGTTCATAGCGAATTTCCGCCGCCAACTTCGGGTGGAGAGACAAATTTCGTTAGAGCTTCGGTATGGCAGAGGAAATAGTTTTGACTTGAAGTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

128

Amino Acids

14.75

Weight (kDa)

10.32

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 86 - 116 1.2e-09 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 31, 170, 301, 304
AclWI GGATC 2 cut(s) 261, 276
AcoI YGGCCR 2 cut(s) 109, 151
AcsI RAATTY 2 cut(s) 295, 327
AcuI CTGAAG 1 cut(s) 212
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 2 cut(s) 40, 313
AgsI TTSAA 2 cut(s) 148, 373
AhdI GACNNNNNGTC 3 cut(s) 16, 208, 373
AjnI CCWGG 1 cut(s) 157
AluBI AGCT 5 cut(s) 60, 144, 192, 243, 340
AluI AGCT 5 cut(s) 60, 144, 192, 243, 340
Alw26I GTCTC 2 cut(s) 179, 316
AlwI GGATC 2 cut(s) 261, 276
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 2 cut(s) 109, 151
ApoI RAATTY 2 cut(s) 295, 327
Asp700I GAANNNNTTC 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 205
AxyI CCTNAGG 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 204
BccI CCATC 1 cut(s) 256
BceAI ACGGC 2 cut(s) 96, 166
BciT130I CCWGG 1 cut(s) 159
BcoDI GTCTC 2 cut(s) 179, 316
BfaI CTAG 2 cut(s) 123, 216
BisI GCNGC 3 cut(s) 31, 171, 304
BlsI GCNGC 3 cut(s) 32, 172, 305
Bme1390I CCNGG 1 cut(s) 159
BmeRI GACNNNNNGTC 3 cut(s) 16, 208, 373
BmeT110I CYCGRG 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 204
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 188, 220
BpuEI CTTGAG 1 cut(s) 46
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 3 cut(s) 157, 158, 195
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 313
Bse21I CCTNAGG 1 cut(s) 35
BseBI CCWGG 1 cut(s) 159
BseDI CCNNGG 3 cut(s) 157, 158, 195
BseLI CCNNNNNNNGG 2 cut(s) 40, 313
BseRI GAGGAG 1 cut(s) 212
BseX3I CGGCCG 1 cut(s) 109
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 111, 153
BsiEI CGRYCG 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 205
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 2 cut(s) 40, 313
BsmAI GTCTC 2 cut(s) 179, 316
BsmFI GGGAC 1 cut(s) 234
BsnI GGCC 2 cut(s) 111, 153
Bso31I GGTCTC 1 cut(s) 179
BsoBI CYCGRG 1 cut(s) 205
Bsp143I GATC 3 cut(s) 105, 253, 268
BspACI CCGC 4 cut(s) 31, 170, 301, 304
BspANI GGCC 2 cut(s) 111, 153
BspPI GGATC 2 cut(s) 261, 276
BspTNI GGTCTC 1 cut(s) 179
BssECI CCNNGG 3 cut(s) 157, 158, 195
BssMI GATC 3 cut(s) 105, 253, 268
Bst2UI CCWGG 1 cut(s) 159
Bst4CI ACNGT 1 cut(s) 118
BstC8I GCNNGC 1 cut(s) 89
BstDEI CTNAG 1 cut(s) 35
BstENI CCTNNNNNAGG 1 cut(s) 38
BstKTI GATC 3 cut(s) 108, 256, 271
BstMAI GTCTC 2 cut(s) 179, 316
BstMBI GATC 3 cut(s) 105, 253, 268
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 1 cut(s) 150
BstNI CCWGG 1 cut(s) 159
BstSCI CCNGG 1 cut(s) 157
BstV2I GAAGAC 1 cut(s) 204
BstZI CGGCCG 1 cut(s) 109
Bsu36I CCTNAGG 1 cut(s) 35
BsuRI GGCC 2 cut(s) 111, 153
BtgZI GCGATG 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 89
Csp6I GTAC 1 cut(s) 114
CviAII CATG 1 cut(s) 7
CviJI RGCY 9 cut(s) 30, 60, 111, 144, 153, 163, 192, 243, 340
CviKI_1 RGCY 9 cut(s) 30, 60, 111, 144, 153, 163, 192, 243, 340
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 1 cut(s) 35
DpnI GATC 3 cut(s) 107, 255, 270
DpnII GATC 3 cut(s) 105, 253, 268
DriI GACNNNNNGTC 3 cut(s) 16, 208, 373
EaeI YGGCCR 2 cut(s) 109, 151
EagI CGGCCG 1 cut(s) 109
Eam1105I GACNNNNNGTC 3 cut(s) 16, 208, 373
EciI GGCGGA 1 cut(s) 290
EclXI CGGCCG 1 cut(s) 109
Eco31I GGTCTC 1 cut(s) 179
Eco52I CGGCCG 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 212
Eco81I CCTNAGG 1 cut(s) 35
Eco88I CYCGRG 1 cut(s) 205
EcoNI CCTNNNNNAGG 1 cut(s) 38
EcoRII CCWGG 1 cut(s) 157
FaeI CATG 1 cut(s) 10
FaiI YATR 3 cut(s) 8, 290, 348
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FaqI GGGAC 1 cut(s) 234
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 3 cut(s) 31, 171, 304
Fsp4HI GCNGC 3 cut(s) 31, 171, 304
FspBI CTAG 2 cut(s) 123, 216
GluI GCNGC 3 cut(s) 31, 171, 304
HaeIII GGCC 2 cut(s) 111, 153
Hin1II CATG 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 58
HinfI GANTC 2 cut(s) 82, 203
Hpy188I TCNGA 3 cut(s) 196, 231, 253
Hpy188III TCNNGA 2 cut(s) 25, 257
HpyAV CCTTC 2 cut(s) 34, 46
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 150
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 1 cut(s) 10
Kzo9I GATC 3 cut(s) 105, 253, 268
LmnI GCTCC 1 cut(s) 197
LpnPI CCDG 5 cut(s) 73, 143, 144, 171, 202
MaeI CTAG 2 cut(s) 123, 216
MaeII ACGT 1 cut(s) 78
MalI GATC 3 cut(s) 107, 255, 270
MboI GATC 3 cut(s) 105, 253, 268
MboII GAAGA 2 cut(s) 204, 218
MfeI CAATTG 1 cut(s) 134
MluCI AATT 4 cut(s) 93, 134, 295, 327
MlyI GAGTC 1 cut(s) 197
MnlI CCTC 6 cut(s) 42, 83, 190, 193, 225, 347
MroXI GAANNNNTTC 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 159
MunI CAATTG 1 cut(s) 134
MvaI CCWGG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 150
NdeII GATC 3 cut(s) 105, 253, 268
NlaIII CATG 1 cut(s) 10
PasI CCCWGGG 1 cut(s) 158
PdmI GAANNNNTTC 1 cut(s) 97
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 3 cut(s) 32, 172, 305
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 157
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SatI GCNGC 3 cut(s) 31, 171, 304
Sau3AI GATC 3 cut(s) 105, 253, 268
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 159
SetI ASST 9 cut(s) 45, 57, 62, 75, 81, 146, 194, 245, 342
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
Sse9I AATT 4 cut(s) 93, 134, 295, 327
SsiI CCGC 4 cut(s) 31, 170, 301, 304
SspMI CTAG 2 cut(s) 123, 216
StyD4I CCNGG 1 cut(s) 157
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 1 cut(s) 81
TaqI TCGA 2 cut(s) 101, 256
TasI AATT 4 cut(s) 93, 134, 295, 327
TauI GCSGC 3 cut(s) 33, 173, 306
TfiI GAWTC 1 cut(s) 82
TspDTI ATGAA 2 cut(s) 276, 277
XagI CCTNNNNNAGG 1 cut(s) 38
XapI RAATTY 2 cut(s) 295, 327
XmnI GAANNNNTTC 1 cut(s) 97
XspI CTAG 2 cut(s) 123, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.