pycom16g23130

Auxin canalisation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006859
Physical Location & Seq
Forward (+)
23734751 .. 23737250
2500 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1002 bp
ATGTCCAATCATATCAACGAGGACAGGGAACAAGATGTTCTCAGTGAAAAGACTAGGCACATCAGAAGTTACGTGGCTATATATGATGGTGATTTTTGCATGTCTATTTATATGTCCCAGTTGCTGCTGAGTTGCATGGATTCAAATTTCACTACTTCAACTCCGGAGTCACATCCTGAGGCAACGGATTTTCTGTCGCTTGCATGGTGCAACTTTGCAGTGCAAGCTTTCCAACCAGACCAGCAAGATCGATCAGTGGTTCTACTCGATGATCCGATTGACAAGTTTAAGAGCCACAACAAGACCATGCATCCAGAATTGAACTACACCGGCTTTCTTCGGAAAAAATGGATGTCATGGAAGATAGTGCCATTTGAGAACATGTCGATCAAGAAATGGCTTAAAGACATTAAGCAAAGGCGGAAAGAGGAGCAGAGGCTGCAAAGAGCTGAAGTCCATGTAGCGGCTGCACTTGTTGCTATTGCAGCTGAAAAATCAGAAAATGATGGGTCAAGGAAGCTGCCATCGCTTCTGCAGCCATGCTGGTGGCGGCCCAGTAAGCACAACATAATTTTTTTTCATTGGTGTGGTTTGAAACATATAGCATTGAAGGGAGCAGCTACATTGAAAGAAAGATCAGGATGCCGCAAGAAGAGACTAAATGGGACTGCACCTGTGATGCCTATCGAGGACAACCCTGACGCTGAGGAGGTCGATTTCGACTTTGACAAGCATAGATCAATGCTTGCAATAGGTTCCCAGCTCACCACTGAAACACCTGACGGTATCATACTGGACATGTACGCAGAGCTGTATAAAGATTCAGAAGCGGGTGAGAATGGTATGGGTTATCTCATCGTGTTAACGACAAGTAAGGGGGCGTTCAAGCTAGACATGGAAGCCGACTATCAACGGTATAAGACAAGGGCGACAACCATTAACCATATGCTCATGGTCTCTACTTCATTTATGAAATATCATCTTCAATACTACAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_canalis PH_2

Protein Analysis

334

Amino Acids

38.55

Weight (kDa)

7.19

Isoelectric Point (pI)

58.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_canalis PF05703 93 - 172 1.2e-13 Auxin canalisation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 5 cut(s) 421, 464, 550, 646, 830
AclWI GGATC 1 cut(s) 266
AcsI RAATTY 1 cut(s) 145
AcuI CTGAAG 1 cut(s) 471
AfaI GTAC 1 cut(s) 803
AfiI CCNNNNNNNGG 1 cut(s) 463
AflIII ACRYGT 2 cut(s) 381, 798
AgsI TTSAA 8 cut(s) 144, 159, 322, 595, 610, 628, 886, 986
AluBI AGCT 8 cut(s) 227, 449, 488, 520, 620, 763, 811, 889
AluI AGCT 8 cut(s) 227, 449, 488, 520, 620, 763, 811, 889
Alw26I GTCTC 2 cut(s) 649, 961
AlwI GGATC 1 cut(s) 266
AlwNI CAGNNNCTG 2 cut(s) 124, 439
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 1 cut(s) 551
ApeKI GCWGC 7 cut(s) 124, 439, 467, 485, 520, 535, 617
ApoI RAATTY 1 cut(s) 145
AspS9I GGNCC 1 cut(s) 552
AsuHPI GGTGA 3 cut(s) 101, 757, 845
AxyI CCTNAGG 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 7 cut(s) 111, 426, 454, 497, 507, 547, 629
BccI CCATC 3 cut(s) 80, 500, 532
BcoDI GTCTC 2 cut(s) 649, 961
BfaI CTAG 3 cut(s) 54, 890, 1000
BfmI CTRYAG 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 552
BmiI GGNNCC 1 cut(s) 757
BmrI ACTGGG 2 cut(s) 112, 549
BmsI GCATC 3 cut(s) 319, 632, 669
BmuI ACTGGG 2 cut(s) 112, 549
Bpu10I CCTNAGC 1 cut(s) 705
Bsa29I ATCGAT 1 cut(s) 250
BsaAI YACGTR 1 cut(s) 73
BsaBI GATNNNNATC 1 cut(s) 683
BsaI GGTCTC 1 cut(s) 961
BsaWI WCCGGW 1 cut(s) 163
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 1 cut(s) 463
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 3 cut(s) 118, 555, 798
Bse21I CCTNAGG 1 cut(s) 177
Bse8I GATNNNNATC 1 cut(s) 683
BseAI TCCGGA 1 cut(s) 163
BseCI ATCGAT 1 cut(s) 250
BseGI GGATG 4 cut(s) 172, 310, 357, 647
BseJI GATNNNNATC 1 cut(s) 683
BseLI CCNNNNNNNGG 1 cut(s) 463
BseMII CTCAG 4 cut(s) 55, 119, 168, 696
BseNI ACTGG 3 cut(s) 118, 555, 798
BseRI GAGGAG 2 cut(s) 443, 722
BseXI GCAGC 7 cut(s) 111, 426, 454, 497, 507, 547, 629
BseYI CCCAGC 1 cut(s) 759
BsgI GTGCAG 2 cut(s) 453, 654
BshFI GGCC 1 cut(s) 553
BshVI ATCGAT 1 cut(s) 250
BsiSI CCGG 2 cut(s) 164, 330
BslFI GGGAC 2 cut(s) 100, 679
BslI CCNNNNNNNGG 1 cut(s) 463
BsmAI GTCTC 2 cut(s) 649, 961
BsmFI GGGAC 2 cut(s) 100, 679
BsnI GGCC 1 cut(s) 553
Bso31I GGTCTC 1 cut(s) 961
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 6 cut(s) 247, 251, 271, 387, 635, 737
BspACI CCGC 5 cut(s) 421, 464, 550, 646, 830
BspANI GGCC 1 cut(s) 553
BspCNI CTCAG 4 cut(s) 54, 120, 169, 697
BspDI ATCGAT 1 cut(s) 250
BspEI TCCGGA 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 757
BspMAI CTGCAG 1 cut(s) 537
BspPI GGATC 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 961
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 3 cut(s) 118, 555, 798
BssAI RCCGGY 1 cut(s) 329
BssMI GATC 6 cut(s) 247, 251, 271, 387, 635, 737
Bst4CI ACNGT 2 cut(s) 785, 915
Bst6I CTCTTC 1 cut(s) 647
BstAPI GCANNNNNTGC 2 cut(s) 439, 476
BstBAI YACGTR 1 cut(s) 73
BstC8I GCNNGC 3 cut(s) 201, 225, 747
BstDEI CTNAG 4 cut(s) 41, 128, 177, 705
BstF5I GGATG 4 cut(s) 172, 310, 357, 647
BstKTI GATC 6 cut(s) 250, 254, 274, 390, 638, 740
BstMAI GTCTC 2 cut(s) 649, 961
BstMBI GATC 6 cut(s) 247, 251, 271, 387, 635, 737
BstMWI GCNNNNNNNGC 7 cut(s) 224, 439, 476, 485, 526, 535, 559
BstNSI RCATGY 3 cut(s) 103, 385, 802
BstSFI CTRYAG 1 cut(s) 533
BstV1I GCAGC 7 cut(s) 111, 426, 454, 497, 507, 547, 629
BstXI CCANNNNNNTGG 1 cut(s) 546
Bsu15I ATCGAT 1 cut(s) 250
Bsu36I CCTNAGG 1 cut(s) 177
BsuRI GGCC 1 cut(s) 553
BsuTUI ATCGAT 1 cut(s) 250
BtgZI GCGATG 1 cut(s) 510
BtsCI GGATG 4 cut(s) 172, 310, 357, 647
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 4 cut(s) 49, 225, 261, 768
Cac8I GCNNGC 3 cut(s) 201, 225, 747
CaiI CAGNNNCTG 2 cut(s) 124, 439
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 1 cut(s) 552
ClaI ATCGAT 1 cut(s) 250
CseI GACGC 1 cut(s) 710
Csp6I GTAC 1 cut(s) 802
CviQI GTAC 1 cut(s) 802
DdeI CTNAG 4 cut(s) 41, 128, 177, 705
DpnI GATC 6 cut(s) 249, 253, 273, 389, 637, 739
DpnII GATC 6 cut(s) 247, 251, 271, 387, 635, 737
Eam1104I CTCTTC 1 cut(s) 647
EarI CTCTTC 1 cut(s) 647
EciI GGCGGA 1 cut(s) 436
Eco31I GGTCTC 1 cut(s) 961
Eco57I CTGAAG 1 cut(s) 471
Eco81I CCTNAGG 1 cut(s) 177
EcoT22I ATGCAT 1 cut(s) 312
FaqI GGGAC 2 cut(s) 100, 679
FauI CCCGC 1 cut(s) 823
FauNDI CATATG 1 cut(s) 945
FokI GGATG 4 cut(s) 159, 297, 364, 654
FspBI CTAG 3 cut(s) 54, 890, 1000
GsaI CCCAGC 1 cut(s) 763
HaeIII GGCC 1 cut(s) 553
HapII CCGG 2 cut(s) 164, 330
HgaI GACGC 1 cut(s) 710
HincII GTYRAC 1 cut(s) 864
HindII GTYRAC 1 cut(s) 864
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 3 cut(s) 140, 167, 821
HpaI GTTAAC 1 cut(s) 864
HpaII CCGG 2 cut(s) 164, 330
HphI GGTGA 3 cut(s) 101, 757, 845
Hpy166II GTNNAC 1 cut(s) 864
Hpy188I TCNGA 5 cut(s) 65, 276, 342, 499, 826
Hpy188III TCNNGA 5 cut(s) 164, 176, 314, 391, 639
Hpy8I GTNNAC 1 cut(s) 864
HpyAV CCTTC 1 cut(s) 604
HpyCH4III ACNGT 2 cut(s) 785, 915
HpyCH4IV ACGT 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 7 cut(s) 224, 439, 476, 485, 526, 535, 559
HpyF3I CTNAG 4 cut(s) 41, 128, 177, 705
HpySE526I ACGT 1 cut(s) 72
Kpn2I TCCGGA 1 cut(s) 163
KspAI GTTAAC 1 cut(s) 864
Kzo9I GATC 6 cut(s) 247, 251, 271, 387, 635, 737
LmnI GCTCC 2 cut(s) 430, 614
Lsp1109I GCAGC 7 cut(s) 111, 426, 454, 497, 507, 547, 629
LweI GCATC 3 cut(s) 319, 632, 669
MaeI CTAG 3 cut(s) 54, 890, 1000
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 2 cut(s) 68, 168
MalI GATC 6 cut(s) 249, 253, 273, 389, 637, 739
MboI GATC 6 cut(s) 247, 251, 271, 387, 635, 737
MboII GAAGA 4 cut(s) 329, 373, 664, 974
MluCI AATT 3 cut(s) 145, 317, 570
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 256
MnlI CCTC 7 cut(s) 13, 172, 421, 429, 682, 700, 703
Mph1103I ATGCAT 1 cut(s) 312
MroI TCCGGA 1 cut(s) 163
MseI TTAA 5 cut(s) 288, 402, 411, 863, 939
MslI CAYNNNNRTG 2 cut(s) 544, 585
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 2 cut(s) 164, 330
MwoI GCNNNNNNNGC 7 cut(s) 224, 439, 476, 485, 526, 535, 559
NdeI CATATG 1 cut(s) 945
NdeII GATC 6 cut(s) 247, 251, 271, 387, 635, 737
NlaIV GGNNCC 1 cut(s) 757
NmuCI GTSAC 1 cut(s) 168
NsiI ATGCAT 1 cut(s) 312
NspI RCATGY 3 cut(s) 103, 385, 802
PciI ACATGT 2 cut(s) 381, 798
PfeI GAWTC 2 cut(s) 140, 821
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
Ppu21I YACGTR 1 cut(s) 73
PscI ACATGT 2 cut(s) 381, 798
PspFI CCCAGC 1 cut(s) 759
PspN4I GGNNCC 1 cut(s) 757
PspPI GGNCC 1 cut(s) 552
PstI CTGCAG 1 cut(s) 537
PstNI CAGNNNCTG 2 cut(s) 124, 439
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 1 cut(s) 803
RsaNI GTAC 1 cut(s) 802
RseI CAYNNNNRTG 2 cut(s) 544, 585
SaqAI TTAA 5 cut(s) 288, 402, 411, 863, 939
Sau3AI GATC 6 cut(s) 247, 251, 271, 387, 635, 737
Sau96I GGNCC 1 cut(s) 552
SchI GAGTC 1 cut(s) 176
SfaNI GCATC 3 cut(s) 319, 632, 669
SfcI CTRYAG 1 cut(s) 533
SmiMI CAYNNNNRTG 2 cut(s) 544, 585
Sse9I AATT 3 cut(s) 145, 317, 570
SsiI CCGC 5 cut(s) 421, 464, 550, 646, 830
SspMI CTAG 3 cut(s) 54, 890, 1000
TaaI ACNGT 2 cut(s) 785, 915
TaiI ACGT 1 cut(s) 75
TaqI TCGA 6 cut(s) 250, 267, 386, 687, 714, 720
TasI AATT 3 cut(s) 145, 317, 570
TauI GCSGC 3 cut(s) 467, 553, 648
TfiI GAWTC 2 cut(s) 140, 821
Tru1I TTAA 5 cut(s) 288, 402, 411, 863, 939
Tru9I TTAA 5 cut(s) 288, 402, 411, 863, 939
TscAI CASTG 4 cut(s) 49, 225, 261, 775
TseFI GTSAC 1 cut(s) 168
TseI GCWGC 7 cut(s) 124, 439, 467, 485, 520, 535, 617
Tsp45I GTSAC 1 cut(s) 168
TspDTI ATGAA 3 cut(s) 569, 954, 986
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 4 cut(s) 49, 225, 261, 775
XapI RAATTY 1 cut(s) 145
XceI RCATGY 3 cut(s) 103, 385, 802
XspI CTAG 3 cut(s) 54, 890, 1000
Zsp2I ATGCAT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.