Rh4DG119900

Auxin canalisation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0006859
Physical Location & Seq
Reverse (-)
19021427 .. 19024789
3363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG119900.1

Sequence Viewer

Length: 939 bp
ATGAATATAATGATGCAGCTTTTAGATCCCAGGTTCATGGATTCGAATTTCAGCCCAAGCCCTACGGAGGCACATCCTGATACGATGGATTTTCTGTCTCTTGCATGGTGCAATTTTGCAGTACAAGCTTTGCAACCAGAGCAACAAGACCACCAATCTCTAGTTTCGTTAAGTGATCCAATTATCAAGTTTGAGAGCCAAAACATGACTCAAATCACGAAGTTGGACAAGAATGCAAAGACGGATGGTGCAGATGCCAAGCCCTTACCACCATGGAAATCTAATGATGTGAAGTCATGGATATGGATGCAACAAGCCATGCATCCAGAATTGAACTACAACAGCTATTTCCGAAAGAAATGGCTGTCATGGAAGATAGTGCCATTCAAGAACATGTCGATCAAGAAGTGGCTGAAGGAGATTAAAGAAAGGCGGAAAGAGGAACACAGGTTGCAGAGAGCTGAAGTCCATGCAGCTATATCTATGGCAGGTGTGGCGGCAGCGCTTGCTACCATTGCGGCTGAAAGCTCTGCAAAGAATGAGAGCACTGCCAAGGAAGCAGCTGTGGCTTCTGCAGCTGCTCTGGTGGCAGCCCAGTGTGCAAAAGTTGCAGAAGCAATGGGAGCAAAAAAGGAACAGATCAGCAGTGTAATAGGTTCAGCCATGAGTAGTACAAGTGCAAGCGATATCTTAACCCTCACAGCTGCAGCTGCAACATCATTAAAAGGAGCAGCTACACTTAAAGCAAGATCAGGATGCAAGAATATGCTACATGGGGGTGCACCCGTGCTGCCCATCGAGGAGAGCCATGAGGTTGACTTCGACTTTGAAAAGTGTAGGTCAATGCTCGGAATGGGTGCGCACCTCAGCATCGAAACACCAGATGGTTTGTATTCCGACTTAATGCATCTACATTTTCTGACAAGAAGGGTAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_canalis PH_2

Protein Analysis

312

Amino Acids

34.3

Weight (kDa)

7.72

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_canalis PF05703 25 - 268 1.1e-72 Auxin canalisation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 479
Acc16I TGCGCA 1 cut(s) 861
Acc36I ACCTGC 1 cut(s) 479
AciI CCGC 3 cut(s) 433, 497, 518
AclWI GGATC 2 cut(s) 20, 170
AcsI RAATTY 1 cut(s) 46
AcuI CTGAAG 2 cut(s) 434, 483
AfaI GTAC 2 cut(s) 123, 673
AfeI AGCGCT 1 cut(s) 504
AfiI CCNNNNNNNGG 1 cut(s) 67
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 3 cut(s) 334, 388, 830
AjnI CCWGG 1 cut(s) 29
Alw21I GWGCWC 2 cut(s) 548, 784
Alw26I GTCTC 1 cut(s) 102
Alw44I GTGCAC 1 cut(s) 780
AlwI GGATC 2 cut(s) 20, 170
Aor51HI AGCGCT 1 cut(s) 504
ApaLI GTGCAC 1 cut(s) 780
ApoI RAATTY 1 cut(s) 46
ArsI GACNNNNNNTTYG 2 cut(s) 809, 841
AspLEI GCGC 2 cut(s) 505, 862
AsuII TTCGAA 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 784
Bbv12I GWGCWC 2 cut(s) 548, 784
BbvCI CCTCAGC 1 cut(s) 866
BccI CCATC 4 cut(s) 79, 239, 803, 878
BciT130I CCWGG 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 102
BfaI CTAG 1 cut(s) 161
BfmI CTRYAG 2 cut(s) 573, 705
BfoI RGCGCY 1 cut(s) 506
BfuAI ACCTGC 1 cut(s) 479
Bme1390I CCNGG 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 31
BmrI ACTGGG 1 cut(s) 589
BmsI GCATC 7 cut(s) 3, 244, 297, 331, 746, 879, 916
BmuI ACTGGG 1 cut(s) 589
Bpu10I CCTNAGC 1 cut(s) 866
Bpu14I TTCGAA 1 cut(s) 44
BsaJI CCNNGG 3 cut(s) 29, 272, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 595
Bse3DI GCAATG 2 cut(s) 513, 624
BseBI CCWGG 1 cut(s) 31
BseDI CCNNGG 3 cut(s) 29, 272, 552
BseGI GGATG 5 cut(s) 73, 250, 312, 322, 761
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 2 cut(s) 513, 624
BseMII CTCAG 1 cut(s) 880
BseNI ACTGG 1 cut(s) 595
BseRI GAGGAG 1 cut(s) 815
BseSI GKGCMC 1 cut(s) 784
BsgI GTGCAG 1 cut(s) 270
BsiHKAI GWGCWC 2 cut(s) 548, 784
BslI CCNNNNNNNGG 1 cut(s) 67
BsmAI GTCTC 1 cut(s) 102
BsmI GAATGC 1 cut(s) 238
Bsp119I TTCGAA 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 548, 784
Bsp143I GATC 5 cut(s) 25, 175, 399, 639, 749
Bsp19I CCATGG 1 cut(s) 272
BspACI CCGC 3 cut(s) 433, 497, 518
BspCNI CTCAG 1 cut(s) 879
BspMAI CTGCAG 2 cut(s) 577, 709
BspMI ACCTGC 1 cut(s) 479
BspPI GGATC 2 cut(s) 20, 170
BspT104I TTCGAA 1 cut(s) 44
BsrDI GCAATG 2 cut(s) 513, 624
BsrI ACTGG 1 cut(s) 595
BssECI CCNNGG 3 cut(s) 29, 272, 552
BssMI GATC 5 cut(s) 25, 175, 399, 639, 749
BssT1I CCWWGG 2 cut(s) 272, 552
Bst2UI CCWGG 1 cut(s) 31
BstAPI GCANNNNNTGC 2 cut(s) 506, 608
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 2 cut(s) 507, 682
BstDEI CTNAG 1 cut(s) 866
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 5 cut(s) 73, 250, 312, 322, 761
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 2 cut(s) 505, 862
BstKTI GATC 5 cut(s) 28, 178, 402, 642, 752
BstMAI GTCTC 1 cut(s) 102
BstMBI GATC 5 cut(s) 25, 175, 399, 639, 749
BstNI CCWGG 1 cut(s) 31
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 1 cut(s) 29
BstSFI CTRYAG 2 cut(s) 573, 705
BstSLI GKGCMC 1 cut(s) 784
BstX2I RGATCY 1 cut(s) 25
BstXI CCANNNNNNTGG 1 cut(s) 37
BstYI RGATCY 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 272
BtsCI GGATG 5 cut(s) 73, 250, 312, 322, 761
BtsI GCAGTG 2 cut(s) 546, 652
BtsIMutI CAGTG 3 cut(s) 546, 602, 652
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 2 cut(s) 507, 682
CfoI GCGC 2 cut(s) 505, 862
Csp6I GTAC 2 cut(s) 122, 672
CviQI GTAC 2 cut(s) 122, 672
DdeI CTNAG 1 cut(s) 866
DpnI GATC 5 cut(s) 27, 177, 401, 641, 751
DpnII GATC 5 cut(s) 25, 175, 399, 639, 749
EciI GGCGGA 1 cut(s) 448
Eco130I CCWWGG 2 cut(s) 272, 552
Eco32I GATATC 1 cut(s) 688
Eco47III AGCGCT 1 cut(s) 504
Eco57I CTGAAG 2 cut(s) 434, 483
EcoRII CCWGG 1 cut(s) 29
EcoRV GATATC 1 cut(s) 688
EcoT14I CCWWGG 2 cut(s) 272, 552
EcoT22I ATGCAT 2 cut(s) 324, 909
ErhI CCWWGG 2 cut(s) 272, 552
FokI GGATG 5 cut(s) 60, 257, 309, 319, 768
FspAI RTGCGCAY 1 cut(s) 861
FspBI CTAG 1 cut(s) 161
FspI TGCGCA 1 cut(s) 861
GlaI GCGC 2 cut(s) 504, 861
HaeII RGCGCY 1 cut(s) 506
HhaI GCGC 2 cut(s) 505, 862
Hin6I GCGC 2 cut(s) 503, 860
HinP1I GCGC 2 cut(s) 503, 860
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 2 cut(s) 41, 208
Hpy166II GTNNAC 2 cut(s) 782, 817
Hpy188I TCNGA 4 cut(s) 353, 851, 898, 921
Hpy188III TCNNGA 6 cut(s) 77, 217, 326, 388, 403, 753
Hpy8I GTNNAC 2 cut(s) 782, 817
HpyAV CCTTC 2 cut(s) 409, 921
HpyF3I CTNAG 1 cut(s) 866
HspAI GCGC 2 cut(s) 503, 860
Kzo9I GATC 5 cut(s) 25, 175, 399, 639, 749
LmnI GCTCC 2 cut(s) 623, 728
LweI GCATC 7 cut(s) 3, 244, 297, 331, 746, 879, 916
MaeI CTAG 1 cut(s) 161
MalI GATC 5 cut(s) 27, 177, 401, 641, 751
MboI GATC 5 cut(s) 25, 175, 399, 639, 749
MboII GAAGA 1 cut(s) 385
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 2 cut(s) 548, 784
MluCI AATT 4 cut(s) 46, 112, 180, 329
MlyI GAGTC 1 cut(s) 202
MmeI TCCRAC 2 cut(s) 204, 921
MnlI CCTC 6 cut(s) 61, 433, 707, 793, 805, 875
Mph1103I ATGCAT 2 cut(s) 324, 909
MseI TTAA 6 cut(s) 170, 423, 692, 722, 741, 902
MslI CAYNNNNRTG 2 cut(s) 301, 777
MspA1I CMGCKG 4 cut(s) 563, 578, 704, 710
MspR9I CCNGG 1 cut(s) 31
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 31
NcoI CCATGG 1 cut(s) 272
NdeII GATC 5 cut(s) 25, 175, 399, 639, 749
NsbI TGCGCA 1 cut(s) 861
NsiI ATGCAT 2 cut(s) 324, 909
NspI RCATGY 1 cut(s) 397
NspV TTCGAA 1 cut(s) 44
PaqCI CACCTGC 1 cut(s) 479
PciI ACATGT 1 cut(s) 393
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 41
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PscI ACATGT 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 29
PspGI CCWGG 1 cut(s) 29
PstI CTGCAG 2 cut(s) 577, 709
PsuI RGATCY 1 cut(s) 25
PvuII CAGCTG 4 cut(s) 563, 578, 704, 710
RsaI GTAC 2 cut(s) 123, 673
RsaNI GTAC 2 cut(s) 122, 672
RseI CAYNNNNRTG 2 cut(s) 301, 777
SaqAI TTAA 6 cut(s) 170, 423, 692, 722, 741, 902
Sau3AI GATC 5 cut(s) 25, 175, 399, 639, 749
SchI GAGTC 1 cut(s) 202
ScrFI CCNGG 1 cut(s) 31
SduI GDGCHC 2 cut(s) 548, 784
SfaNI GCATC 7 cut(s) 3, 244, 297, 331, 746, 879, 916
SfcI CTRYAG 2 cut(s) 573, 705
SfuI TTCGAA 1 cut(s) 44
SmiMI CAYNNNNRTG 2 cut(s) 301, 777
Sse9I AATT 4 cut(s) 46, 112, 180, 329
SsiI CCGC 3 cut(s) 433, 497, 518
SspMI CTAG 1 cut(s) 161
StyD4I CCNGG 1 cut(s) 29
StyI CCWWGG 2 cut(s) 272, 552
TaqI TCGA 5 cut(s) 44, 398, 798, 822, 873
TasI AATT 4 cut(s) 46, 112, 180, 329
TatI WGTACW 2 cut(s) 121, 671
TauI GCSGC 2 cut(s) 500, 521
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 6 cut(s) 170, 423, 692, 722, 741, 902
Tru9I TTAA 6 cut(s) 170, 423, 692, 722, 741, 902
TscAI CASTG 3 cut(s) 553, 602, 652
TspDTI ATGAA 2 cut(s) 17, 25
TspGWI ACGGA 2 cut(s) 80, 257
TspRI CASTG 3 cut(s) 553, 602, 652
VneI GTGCAC 1 cut(s) 780
XapI RAATTY 1 cut(s) 46
XceI RCATGY 1 cut(s) 397
XspI CTAG 1 cut(s) 161
Zsp2I ATGCAT 2 cut(s) 324, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.