pycom16g25080

PAP_fibrillin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011205
Physical Location & Seq
Forward (+)
26822029 .. 26825095
3067 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1062 bp
ATGGCTCTCACTCTGATCTTCACCTCAAACTCCTCTCTCCTCTCCAAAACCCTCGTCAATTCCCCATCCTCATTTCCAACCCTTAATTCTCAATCTCTCTCTCTCCTCTCCCCAAACCCTAAACCCAAGCTCCCCTCCCTCCGCCTACGTTCCTCTCTCTCCGACGACACCCCCGCCGATGGCGACCGCCCCACCAATATCACCGACGAGTGGGGCGAGAAGACCGACCCCGAGCCGGAGCCGCTGTCCAAAATCTCCGAATCGGACCCTCCGCAGAACGAGGACGAGTGGGGAGGCGGAGGAGATGAGGTTGTAGAAGTTGGGAATGGAAGTGCAGCCAAATCTGAGGTTGTGATTGAGGAGAAGGTGGACACTAAGCTTACTGAGCTCAAGAGGGGTTTGGTAGACACGGTTTATGGGACTGAATTCGGGTTTCGGGCTGGGTCTGAGGTGAGAGCGGAGGTTATGGAGTTCGTAAGCCAGTTGGAGGCGGCAAATCCCAACCCGGCTCCGACGGAGAATCTGGGCCTCCTTGATGGCAACTGGGTTTTGCTGTACACTGCATCTTCCGAGCTGTTGCCACTTCTGGCAACAGGTAGTACACCTTTTTTGAAGGTAAATAAAATAACCCAAGCGATCAACACAAGCAGCTTGACTATTTTGAACTCCACCACGTTGTCCAGCCCTTTCGCTAGTTTCTCATTCAGTGCATCTGCTAGTTTTGAAGTTAGAAGTCCTTTAAGAATCCAGGTTGAATTCAAGGAAGGCAGCTTTCAGCCTCCAGAGATTAAATCAAGCATTGATCTCCCGCAAGAAGTGGATGTATTTGGGCAGAAAGTCAATTTATCGCCCGTCCAGCAAACCCTCAGTCCTTTGCAGGACGCCGTTGCAGGTATCTCTAGGACTATTTCTGGTCAGCCACCGCTTAAGGTTTCCATTCCAGGTGAGCGGACGAAGTCTTGGCTTGTTATTACATACCTTGATGAGGATCTCCGGATCTCAAGGGGGGATGGTGGTCTTTTTGTGCTTGCTAGAGAAGGGAGCGTGCTTTTATATCAGTGA

Protein Analysis

354

Amino Acids

38.03

Weight (kDa)

4.54

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 129 - 344 3e-70 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35490
fragaria_vesca FvH4_5g22910 FvH4_5g22910 FvH4_5g22910
malus_domestica MD06G1025700.v1.1 MD16G1280700.v1.1
prunus_persica Prupe.5G029300_v2.0.a1
pyrus_communis pycom06g02090 pycom16g25080
rosa_chinensis RchiOBHm_Chr7g0211641
rosa_laevigata RLG00000002988
rosa_multiflora Rmu_sc0003760.1_g000015 Rmu_sc0004232.1_g000010
rosa_roxburghii Rroxscaffold_3G00247650
rosa_rugosa Rorug07G0126400
rosa_samantha Rh7AG259200 Rh7BG253500 Rh7CG276200 Rh7DG266800
rosa_wichuraiana Rw7G022160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 881
AccBSI CCGCTC 2 cut(s) 458, 949
AccI GTMKAC 1 cut(s) 405
AccIII TCCGGA 1 cut(s) 993
AclWI GGATC 2 cut(s) 996, 1004
AcsI RAATTY 2 cut(s) 425, 755
AcyI GRCGYC 1 cut(s) 882
AfaI GTAC 2 cut(s) 557, 601
AfiI CCNNNNNNNGG 5 cut(s) 179, 210, 235, 487, 985
AflII CTTAAG 1 cut(s) 926
AgsI TTSAA 5 cut(s) 613, 664, 725, 755, 760
AjnI CCWGG 2 cut(s) 747, 940
AjuI GAANNNNNNNTTGG 2 cut(s) 70, 102
AluBI AGCT 6 cut(s) 130, 379, 388, 574, 651, 771
AluI AGCT 6 cut(s) 130, 379, 388, 574, 651, 771
Alw21I GWGCWC 1 cut(s) 390
AlwI GGATC 2 cut(s) 996, 1004
Ama87I CYCGRG 1 cut(s) 230
Aor13HI TCCGGA 1 cut(s) 993
AoxI GGCC 1 cut(s) 526
ApeKI GCWGC 3 cut(s) 335, 648, 768
ApoI RAATTY 2 cut(s) 425, 755
ArsI GACNNNNNNTTYG 4 cut(s) 39, 71, 853, 885
AspS9I GGNCC 2 cut(s) 265, 526
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 4 cut(s) 13, 193, 463, 956
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 3 cut(s) 347, 660, 780
BccI CCATC 4 cut(s) 73, 173, 530, 1004
BceAI ACGGC 1 cut(s) 869
BciT130I CCWGG 2 cut(s) 749, 942
BcnI CCSGG 1 cut(s) 506
BfaI CTAG 4 cut(s) 693, 717, 900, 1032
BfrI CTTAAG 1 cut(s) 926
BfuAI ACCTGC 1 cut(s) 881
BisI GCNGC 5 cut(s) 242, 336, 492, 649, 769
BlsI GCNGC 5 cut(s) 243, 337, 493, 650, 770
Bme1390I CCNGG 3 cut(s) 506, 749, 942
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 2 cut(s) 265, 526
BmiI GGNNCC 3 cut(s) 240, 267, 510
BmrFI CCNGG 3 cut(s) 506, 749, 942
BmrI ACTGGG 1 cut(s) 553
BmsI GCATC 2 cut(s) 572, 719
BmuI ACTGGG 1 cut(s) 553
BpiI GAAGAC 1 cut(s) 227
BpmI CTGGAG 1 cut(s) 765
BpuEI CTTGAG 2 cut(s) 374, 985
BpuMI CCSGG 1 cut(s) 506
BsaBI GATNNNNATC 1 cut(s) 987
BsaHI GRCGYC 1 cut(s) 882
BsaWI WCCGGW 1 cut(s) 993
BsaXI ACNNNNNCTCC 6 cut(s) 114, 144, 230, 260, 294, 324
Bsc4I CCNNNNNNNGG 5 cut(s) 179, 210, 235, 487, 985
Bse1I ACTGG 2 cut(s) 481, 548
Bse8I GATNNNNATC 1 cut(s) 987
BseAI TCCGGA 1 cut(s) 993
BseBI CCWGG 2 cut(s) 749, 942
BseGI GGATG 3 cut(s) 65, 826, 1015
BseJI GATNNNNATC 1 cut(s) 987
BseLI CCNNNNNNNGG 5 cut(s) 179, 210, 235, 487, 985
BseMII CTCAG 4 cut(s) 336, 375, 438, 880
BseNI ACTGG 2 cut(s) 481, 548
BseRI GAGGAG 5 cut(s) 22, 29, 95, 315, 374
BseXI GCAGC 3 cut(s) 347, 660, 780
BseYI CCCAGC 1 cut(s) 440
BsgI GTGCAG 1 cut(s) 354
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 528
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 3 cut(s) 236, 506, 994
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 5 cut(s) 179, 210, 235, 487, 985
BsmFI GGGAC 1 cut(s) 433
BsnI GGCC 1 cut(s) 528
BsoBI CYCGRG 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 390
Bsp13I TCCGGA 1 cut(s) 993
Bsp1407I TGTACA 1 cut(s) 555
Bsp143I GATC 5 cut(s) 15, 636, 802, 988, 996
BspANI GGCC 1 cut(s) 528
BspCNI CTCAG 4 cut(s) 337, 376, 439, 879
BspEI TCCGGA 1 cut(s) 993
BspLI GGNNCC 3 cut(s) 240, 267, 510
BspMI ACCTGC 1 cut(s) 881
BspPI GGATC 2 cut(s) 996, 1004
BspTI CTTAAG 1 cut(s) 926
BsrBI CCGCTC 2 cut(s) 458, 949
BsrGI TGTACA 1 cut(s) 555
BsrI ACTGG 2 cut(s) 481, 548
BssMI GATC 5 cut(s) 15, 636, 802, 988, 996
BssNI GRCGYC 1 cut(s) 882
Bst2UI CCWGG 2 cut(s) 749, 942
Bst4CI ACNGT 1 cut(s) 412
BstACI GRCGYC 1 cut(s) 882
BstAFI CTTAAG 1 cut(s) 926
BstAUI TGTACA 1 cut(s) 555
BstC8I GCNNGC 2 cut(s) 1029, 1046
BstDEI CTNAG 5 cut(s) 345, 375, 384, 447, 866
BstENI CCTNNNNNAGG 1 cut(s) 983
BstF5I GGATG 3 cut(s) 65, 826, 1015
BstKTI GATC 5 cut(s) 18, 639, 805, 991, 999
BstMBI GATC 5 cut(s) 15, 636, 802, 988, 996
BstMCI CGRYCG 1 cut(s) 187
BstMWI GCNNNNNNNGC 3 cut(s) 241, 385, 856
BstNI CCWGG 2 cut(s) 749, 942
BstSCI CCNGG 3 cut(s) 504, 747, 940
BstV1I GCAGC 3 cut(s) 347, 660, 780
BstV2I GAAGAC 1 cut(s) 227
BstX2I RGATCY 2 cut(s) 988, 996
BstYI RGATCY 2 cut(s) 988, 996
BsuRI GGCC 1 cut(s) 528
BtsCI GGATG 3 cut(s) 65, 826, 1015
BtsI GCAGTG 1 cut(s) 558
BtsIMutI CAGTG 2 cut(s) 558, 712
BveI ACCTGC 1 cut(s) 881
Cac8I GCNNGC 2 cut(s) 1029, 1046
Cfr13I GGNCC 2 cut(s) 265, 526
CseI GACGC 1 cut(s) 890
Csp6I GTAC 2 cut(s) 556, 600
CviQI GTAC 2 cut(s) 556, 600
DdeI CTNAG 5 cut(s) 345, 375, 384, 447, 866
DpnI GATC 5 cut(s) 17, 638, 804, 990, 998
DpnII GATC 5 cut(s) 15, 636, 802, 988, 996
EciI GGCGGA 2 cut(s) 131, 312
Ecl136II GAGCTC 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 388
Eco88I CYCGRG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 388
EcoNI CCTNNNNNAGG 1 cut(s) 983
EcoRI GAATTC 2 cut(s) 425, 755
EcoRII CCWGG 2 cut(s) 747, 940
EcoT38I GRGCYC 1 cut(s) 390
FaiI YATR 4 cut(s) 417, 467, 976, 1054
FaqI GGGAC 1 cut(s) 433
FauI CCCGC 2 cut(s) 181, 816
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 5 cut(s) 242, 336, 492, 649, 769
FokI GGATG 3 cut(s) 52, 833, 1022
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 5 cut(s) 242, 336, 492, 649, 769
FspBI CTAG 4 cut(s) 693, 717, 900, 1032
GluI GCNGC 5 cut(s) 242, 336, 492, 649, 769
GsaI CCCAGC 1 cut(s) 444
GsuI CTGGAG 1 cut(s) 765
HaeIII GGCC 1 cut(s) 528
HapII CCGG 3 cut(s) 236, 506, 994
HgaI GACGC 1 cut(s) 890
Hin1I GRCGYC 1 cut(s) 882
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 3 cut(s) 260, 520, 744
HpaII CCGG 3 cut(s) 236, 506, 994
HphI GGTGA 4 cut(s) 13, 193, 463, 956
Hpy166II GTNNAC 4 cut(s) 370, 406, 558, 602
Hpy188I TCNGA 8 cut(s) 15, 163, 259, 265, 346, 448, 513, 571
Hpy188III TCNNGA 3 cut(s) 391, 782, 994
Hpy8I GTNNAC 4 cut(s) 370, 406, 558, 602
Hpy99I CGWCG 3 cut(s) 167, 209, 517
HpyAV CCTTC 4 cut(s) 358, 607, 758, 1031
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4IV ACGT 2 cut(s) 148, 674
HpyCH4V TGCA 5 cut(s) 335, 563, 710, 877, 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 241, 385, 856
HpyF3I CTNAG 5 cut(s) 345, 375, 384, 447, 866
HpySE526I ACGT 2 cut(s) 148, 674
Hsp92I GRCGYC 1 cut(s) 882
Kpn2I TCCGGA 1 cut(s) 993
Kzo9I GATC 5 cut(s) 15, 636, 802, 988, 996
LmnI GCTCC 4 cut(s) 135, 238, 514, 1041
Lsp1109I GCAGC 3 cut(s) 347, 660, 780
LweI GCATC 2 cut(s) 572, 719
MaeI CTAG 4 cut(s) 693, 717, 900, 1032
MaeII ACGT 2 cut(s) 148, 674
MalI GATC 5 cut(s) 17, 638, 804, 990, 998
MbiI CCGCTC 2 cut(s) 458, 949
MboI GATC 5 cut(s) 15, 636, 802, 988, 996
MboII GAAGA 3 cut(s) 10, 232, 558
MflI RGATCY 2 cut(s) 988, 996
MhlI GDGCHC 1 cut(s) 390
MluCI AATT 5 cut(s) 58, 85, 425, 755, 841
MmeI TCCRAC 4 cut(s) 101, 186, 465, 536
MroI TCCGGA 1 cut(s) 993
MseI TTAA 4 cut(s) 84, 740, 789, 927
MspA1I CMGCKG 1 cut(s) 244
MspCI CTTAAG 1 cut(s) 926
MspI CCGG 3 cut(s) 236, 506, 994
MspR9I CCNGG 3 cut(s) 506, 749, 942
MvaI CCWGG 2 cut(s) 749, 942
MwoI GCNNNNNNNGC 3 cut(s) 241, 385, 856
NciI CCSGG 1 cut(s) 506
NdeII GATC 5 cut(s) 15, 636, 802, 988, 996
NlaIV GGNNCC 3 cut(s) 240, 267, 510
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PfeI GAWTC 3 cut(s) 260, 520, 744
PflFI GACNNNGTC 1 cut(s) 955
PkrI GCNGC 5 cut(s) 243, 337, 493, 650, 770
Psp124BI GAGCTC 1 cut(s) 390
Psp6I CCWGG 2 cut(s) 747, 940
PspFI CCCAGC 1 cut(s) 440
PspGI CCWGG 2 cut(s) 747, 940
PspN4I GGNNCC 3 cut(s) 240, 267, 510
PspPI GGNCC 2 cut(s) 265, 526
PsuI RGATCY 2 cut(s) 988, 996
PsyI GACNNNGTC 1 cut(s) 955
RsaI GTAC 2 cut(s) 557, 601
RsaNI GTAC 2 cut(s) 556, 600
SacI GAGCTC 1 cut(s) 390
SaqAI TTAA 4 cut(s) 84, 740, 789, 927
SatI GCNGC 5 cut(s) 242, 336, 492, 649, 769
Sau3AI GATC 5 cut(s) 15, 636, 802, 988, 996
Sau96I GGNCC 2 cut(s) 265, 526
ScrFI CCNGG 3 cut(s) 506, 749, 942
SduI GDGCHC 1 cut(s) 390
SfaNI GCATC 2 cut(s) 572, 719
SinI GGWCC 1 cut(s) 265
SmlI CTYRAG 3 cut(s) 389, 926, 1000
SmoI CTYRAG 3 cut(s) 389, 926, 1000
Sse9I AATT 5 cut(s) 58, 85, 425, 755, 841
SspMI CTAG 4 cut(s) 693, 717, 900, 1032
SstI GAGCTC 1 cut(s) 390
StyD4I CCNGG 3 cut(s) 504, 747, 940
TaaI ACNGT 1 cut(s) 412
TaiI ACGT 2 cut(s) 151, 677
TaqII GACCGA 1 cut(s) 239
TasI AATT 5 cut(s) 58, 85, 425, 755, 841
TatI WGTACW 2 cut(s) 555, 599
TauI GCSGC 2 cut(s) 244, 494
TfiI GAWTC 3 cut(s) 260, 520, 744
Tru1I TTAA 4 cut(s) 84, 740, 789, 927
Tru9I TTAA 4 cut(s) 84, 740, 789, 927
TscAI CASTG 2 cut(s) 565, 712
TseI GCWGC 3 cut(s) 335, 648, 768
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 2 cut(s) 565, 712
Tth111I GACNNNGTC 1 cut(s) 955
Vha464I CTTAAG 1 cut(s) 926
VpaK11BI GGWCC 1 cut(s) 265
XagI CCTNNNNNAGG 1 cut(s) 983
XapI RAATTY 2 cut(s) 425, 755
XmiI GTMKAC 1 cut(s) 405
XspI CTAG 4 cut(s) 693, 717, 900, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.