pycom17g00470

protein At2g33490-like isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001635
Physical Location & Seq
Reverse (-)
327733 .. 331254
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1137 bp
ATGAAGACCTCGCTCGGCATGTTTCGGAGATTCGAACTGCACAAAAATGATGCCAAAGAAAAGAGAGACATTCAACCTTTGGCTCAGGTCGATGAGCTCGCTCAGGCTGCTCAGGACATGCATGATATGAGAACTTGCTATGATAGCTTACTCTCTGCAGCAGCCGCTACAGCAAACAGCGCATATGAATTCTCGGAGTCACTGCGTGAAATGGGTACTTGTCTGCTGGAAAAAACTGCATTGCATGATGATGAAGAGAGTGGTAGAGTATTTCTGATGCTGGGGAGGTTGCAGTATGAACTTCAAAAACTTGTTGATAGCTATCGCTCTCATATATTTTTGACAATTACAAACCCGTCGGAGTCTCTTCTCAATGAACTTCGGACAGTTGAGGAAATGAAGCGACAATGCGATGAAAAAAGAGAAGTGTATGACTACATGGTGGCGCAACAGAAAGAAAAAGGGAGGTCAAAACGTGGAAAAGGTGACAGTTTTACTTTGCAGCAATTGCAAGTAGCTCATGATGAATATGATGAGGAGGCTACATTGTGTGTCTTCCGATTGAAGTCTCTGAAGCAAGGACAGGCTCGAAGTCTTCTAACACAGGCAGCTCGTCACCATGCTGCTCAGTTGAATTTCTTTCGGAAAGGACTTAAATCACTTGAGGCTGTTGAACCACATGTTAGATTGGTTACTGAGGAGCATCATATTGAATACCAATTCAGTGGACTTGAAGACGATGGTGGGGATGATGGTGGGGATGATGTTGAGGATAATGGTGAAAATAGGTATGATCCTACTGAAGATGGAGAATTGAGTTTTAACTATAGGTCAAGCAAGCAGGGGATTGATGTCACCTCTGCATCAAGGAATTCAATGGAGGTTGATGAAGTGGGACCTTTGTCTCCTAAAGCTACAAGATTGGAACATGCAGATATGAATCTAGATAGGAACCAATGGGATATGAGGGCCCCAAGTAGAGAACCAAGAATCGGCAGCCACTCAGCCCCAATTTTTCCGGGGAAGAAGTTTGATCGATCCACTCACCTGCTGAGAAAGCTAGACAATTGCAAGCATCAACACCACGGAAGTCTAATACGTATGTACTGCCCACACCTATTGATCCAAAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BAR BAR_3

Protein Analysis

379

Amino Acids

43.32

Weight (kDa)

5.81

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR PF03114 54 - 227 1.2e-08 BAR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1056
Acc36I ACCTGC 1 cut(s) 1056
AciI CCGC 1 cut(s) 165
AclWI GGATC 3 cut(s) 788, 1032, 1117
AcsI RAATTY 3 cut(s) 188, 634, 871
AcuI CTGAAG 2 cut(s) 593, 822
AdeI CACNNNGTG 1 cut(s) 206
AfaI GTAC 2 cut(s) 217, 1106
AfiI CCNNNNNNNGG 1 cut(s) 992
AflIII ACRYGT 1 cut(s) 679
AgsI TTSAA 8 cut(s) 74, 305, 565, 634, 674, 713, 734, 876
AluBI AGCT 7 cut(s) 97, 147, 321, 518, 611, 914, 1060
AluI AGCT 7 cut(s) 97, 147, 321, 518, 611, 914, 1060
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 4 cut(s) 60, 369, 573, 909
AlwI GGATC 3 cut(s) 788, 1032, 1117
AoxI GGCC 1 cut(s) 969
ApaI GGGCCC 1 cut(s) 973
ApeKI GCWGC 7 cut(s) 107, 158, 161, 502, 608, 623, 996
ApoI RAATTY 3 cut(s) 188, 634, 871
AspLEI GCGC 2 cut(s) 182, 448
AspS9I GGNCC 3 cut(s) 896, 969, 970
AsuC2I CCSGG 1 cut(s) 1020
AsuHPI GGTGA 5 cut(s) 497, 608, 791, 847, 1037
AsuII TTCGAA 1 cut(s) 33
AvaII GGWCC 1 cut(s) 896
BaeGI GKGCMC 1 cut(s) 973
BanII GRGCYC 2 cut(s) 99, 973
BbsI GAAGAC 4 cut(s) 11, 547, 587, 741
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 7 cut(s) 94, 170, 173, 514, 610, 620, 1008
BccI CCATC 3 cut(s) 734, 746, 800
BcnI CCSGG 1 cut(s) 1020
BcoDI GTCTC 4 cut(s) 60, 369, 573, 909
BfaI CTAG 2 cut(s) 944, 1061
BfmI CTRYAG 3 cut(s) 156, 168, 826
BfuAI ACCTGC 1 cut(s) 1056
BisI GCNGC 8 cut(s) 108, 159, 162, 165, 503, 609, 624, 997
BlsI GCNGC 8 cut(s) 109, 160, 163, 166, 504, 610, 625, 998
Bme1390I CCNGG 1 cut(s) 1020
Bme18I GGWCC 1 cut(s) 896
BmgT120I GGNCC 3 cut(s) 896, 969, 970
BmiI GGNNCC 4 cut(s) 897, 953, 971, 972
BmrFI CCNGG 1 cut(s) 1020
BmsI GCATC 5 cut(s) 40, 267, 712, 872, 1084
BoxI GACNNNNGTC 1 cut(s) 901
BpiI GAAGAC 4 cut(s) 11, 547, 587, 741
Bpu10I CCTNAGC 3 cut(s) 84, 102, 111
Bpu14I TTCGAA 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 683
BpuMI CCSGG 1 cut(s) 1020
Bsa29I ATCGAT 1 cut(s) 1036
BsaAI YACGTR 1 cut(s) 1100
BsaBI GATNNNNATC 2 cut(s) 321, 939
BsaJI CCNNGG 2 cut(s) 1019, 1084
Bsc4I CCNNNNNNNGG 1 cut(s) 992
Bse3DI GCAATG 1 cut(s) 239
Bse8I GATNNNNATC 2 cut(s) 321, 939
BseCI ATCGAT 1 cut(s) 1036
BseDI CCNNGG 2 cut(s) 1019, 1084
BseGI GGATG 2 cut(s) 754, 766
BseJI GATNNNNATC 2 cut(s) 321, 939
BseLI CCNNNNNNNGG 1 cut(s) 992
BseMI GCAATG 1 cut(s) 239
BseMII CTCAG 7 cut(s) 98, 116, 125, 641, 687, 1017, 1043
BseRI GAGGAG 2 cut(s) 551, 713
BseSI GKGCMC 1 cut(s) 973
BseXI GCAGC 7 cut(s) 94, 170, 173, 514, 610, 620, 1008
BseYI CCCAGC 1 cut(s) 280
BsgI GTGCAG 1 cut(s) 23
BshFI GGCC 1 cut(s) 971
BshVI ATCGAT 1 cut(s) 1036
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 1 cut(s) 1019
BslFI GGGAC 1 cut(s) 909
BslI CCNNNNNNNGG 1 cut(s) 992
BsmAI GTCTC 4 cut(s) 60, 369, 573, 909
BsmFI GGGAC 1 cut(s) 909
BsnI GGCC 1 cut(s) 971
Bsp119I TTCGAA 1 cut(s) 33
Bsp120I GGGCCC 1 cut(s) 969
Bsp1286I GDGCHC 2 cut(s) 99, 973
Bsp143I GATC 4 cut(s) 793, 1033, 1037, 1122
BspACI CCGC 1 cut(s) 165
BspANI GGCC 1 cut(s) 971
BspCNI CTCAG 7 cut(s) 97, 115, 124, 640, 688, 1016, 1044
BspDI ATCGAT 1 cut(s) 1036
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 4 cut(s) 897, 953, 971, 972
BspMAI CTGCAG 1 cut(s) 160
BspMI ACCTGC 1 cut(s) 1056
BspPI GGATC 3 cut(s) 788, 1032, 1117
BspT104I TTCGAA 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 1019, 1084
BssMI GATC 4 cut(s) 793, 1033, 1037, 1122
Bst4CI ACNGT 2 cut(s) 388, 491
Bst6I CTCTTC 2 cut(s) 249, 372
BstAPI GCANNNNNTGC 1 cut(s) 508
BstBAI YACGTR 1 cut(s) 1100
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 3 cut(s) 99, 839, 1073
BstDEI CTNAG 7 cut(s) 84, 102, 111, 627, 696, 1003, 1052
BstDSI CCRYGG 1 cut(s) 1084
BstF5I GGATG 2 cut(s) 754, 766
BstHHI GCGC 2 cut(s) 182, 448
BstKTI GATC 4 cut(s) 796, 1036, 1040, 1125
BstMAI GTCTC 4 cut(s) 60, 369, 573, 909
BstMBI GATC 4 cut(s) 793, 1033, 1037, 1122
BstMWI GCNNNNNNNGC 7 cut(s) 107, 144, 164, 170, 179, 508, 1057
BstNSI RCATGY 4 cut(s) 22, 121, 683, 932
BstPAI GACNNNNGTC 1 cut(s) 901
BstSCI CCNGG 1 cut(s) 1018
BstSFI CTRYAG 3 cut(s) 156, 168, 826
BstSLI GKGCMC 1 cut(s) 973
BstSNI TACGTA 1 cut(s) 1100
BstV1I GCAGC 7 cut(s) 94, 170, 173, 514, 610, 620, 1008
BstV2I GAAGAC 4 cut(s) 11, 547, 587, 741
BstXI CCANNNNNNTGG 1 cut(s) 725
Bsu15I ATCGAT 1 cut(s) 1036
BsuRI GGCC 1 cut(s) 971
BsuTUI ATCGAT 1 cut(s) 1036
BtgI CCRYGG 1 cut(s) 1084
BtgZI GCGATG 1 cut(s) 426
BtsCI GGATG 2 cut(s) 754, 766
BtsI GCAGTG 1 cut(s) 200
BtsIMutI CAGTG 2 cut(s) 200, 730
BveI ACCTGC 1 cut(s) 1056
Cac8I GCNNGC 3 cut(s) 99, 839, 1073
CciI TCATGA 1 cut(s) 520
CfoI GCGC 2 cut(s) 182, 448
Cfr13I GGNCC 3 cut(s) 896, 969, 970
ClaI ATCGAT 1 cut(s) 1036
Csp6I GTAC 2 cut(s) 216, 1105
CspCI CAANNNNNGTGG 2 cut(s) 1101, 1136
CviAII CATG 9 cut(s) 19, 118, 122, 245, 439, 521, 620, 680, 929
CviQI GTAC 2 cut(s) 216, 1105
DdeI CTNAG 7 cut(s) 84, 102, 111, 627, 696, 1003, 1052
DpnI GATC 4 cut(s) 795, 1035, 1039, 1124
DpnII GATC 4 cut(s) 793, 1033, 1037, 1122
DraIII CACNNNGTG 1 cut(s) 206
Eam1104I CTCTTC 2 cut(s) 249, 372
EarI CTCTTC 2 cut(s) 249, 372
Ecl136II GAGCTC 1 cut(s) 97
Eco105I TACGTA 1 cut(s) 1100
Eco24I GRGCYC 2 cut(s) 99, 973
Eco47I GGWCC 1 cut(s) 896
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 2 cut(s) 593, 822
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 3 cut(s) 896, 969, 970
EcoRI GAATTC 2 cut(s) 188, 871
EcoT22I ATGCAT 1 cut(s) 123
EcoT38I GRGCYC 2 cut(s) 99, 973
FaeI CATG 9 cut(s) 22, 121, 125, 248, 442, 524, 623, 683, 932
FaqI GGGAC 1 cut(s) 909
FatI CATG 9 cut(s) 18, 117, 121, 244, 438, 520, 619, 679, 928
FauNDI CATATG 1 cut(s) 184
Fnu4HI GCNGC 8 cut(s) 108, 159, 162, 165, 503, 609, 624, 997
FokI GGATG 2 cut(s) 761, 773
FriOI GRGCYC 2 cut(s) 99, 973
Fsp4HI GCNGC 8 cut(s) 108, 159, 162, 165, 503, 609, 624, 997
FspBI CTAG 2 cut(s) 944, 1061
GlaI GCGC 2 cut(s) 181, 447
GluI GCNGC 8 cut(s) 108, 159, 162, 165, 503, 609, 624, 997
GsaI CCCAGC 1 cut(s) 284
HaeIII GGCC 1 cut(s) 971
HapII CCGG 1 cut(s) 1019
HhaI GCGC 2 cut(s) 182, 448
Hin1II CATG 9 cut(s) 22, 121, 125, 248, 442, 524, 623, 683, 932
Hin6I GCGC 2 cut(s) 180, 446
HinP1I GCGC 2 cut(s) 180, 446
HinfI GANTC 5 cut(s) 30, 197, 362, 940, 990
HpaII CCGG 1 cut(s) 1019
HphI GGTGA 5 cut(s) 497, 608, 791, 847, 1037
Hpy166II GTNNAC 1 cut(s) 728
Hpy188I TCNGA 8 cut(s) 27, 196, 276, 361, 384, 560, 573, 645
Hpy188III TCNNGA 3 cut(s) 113, 521, 944
Hpy8I GTNNAC 1 cut(s) 728
Hpy99I CGWCG 1 cut(s) 361
HpyCH4III ACNGT 2 cut(s) 388, 491
HpyCH4IV ACGT 2 cut(s) 475, 1099
HpyF10VI GCNNNNNNNGC 7 cut(s) 107, 144, 164, 170, 179, 508, 1057
HpyF3I CTNAG 7 cut(s) 84, 102, 111, 627, 696, 1003, 1052
HpySE526I ACGT 2 cut(s) 475, 1099
Hsp92II CATG 9 cut(s) 22, 121, 125, 248, 442, 524, 623, 683, 932
HspAI GCGC 2 cut(s) 180, 446
Kzo9I GATC 4 cut(s) 793, 1033, 1037, 1122
LmnI GCTCC 1 cut(s) 700
Lsp1109I GCAGC 7 cut(s) 94, 170, 173, 514, 610, 620, 1008
LweI GCATC 5 cut(s) 40, 267, 712, 872, 1084
MaeI CTAG 2 cut(s) 944, 1061
MaeII ACGT 2 cut(s) 475, 1099
MaeIII GTNAC 5 cut(s) 198, 485, 614, 691, 853
MalI GATC 4 cut(s) 795, 1035, 1039, 1124
MboI GATC 4 cut(s) 793, 1033, 1037, 1122
MboII GAAGA 8 cut(s) 16, 266, 359, 547, 587, 746, 815, 1036
MfeI CAATTG 2 cut(s) 506, 1066
MhlI GDGCHC 2 cut(s) 99, 973
MluCI AATT 9 cut(s) 188, 345, 506, 634, 719, 812, 871, 1011, 1066
MlyI GAGTC 2 cut(s) 206, 371
MmeI TCCRAC 1 cut(s) 339
Mph1103I ATGCAT 1 cut(s) 123
MseI TTAA 2 cut(s) 654, 822
MslI CAYNNNNRTG 2 cut(s) 45, 249
MspI CCGG 1 cut(s) 1019
MspR9I CCNGG 1 cut(s) 1020
MunI CAATTG 2 cut(s) 506, 1066
MwoI GCNNNNNNNGC 7 cut(s) 107, 144, 164, 170, 179, 508, 1057
NciI CCSGG 1 cut(s) 1020
NdeI CATATG 1 cut(s) 184
NdeII GATC 4 cut(s) 793, 1033, 1037, 1122
NlaIII CATG 9 cut(s) 22, 121, 125, 248, 442, 524, 623, 683, 932
NlaIV GGNNCC 4 cut(s) 897, 953, 971, 972
NmuCI GTSAC 4 cut(s) 198, 485, 614, 853
NsiI ATGCAT 1 cut(s) 123
NspI RCATGY 4 cut(s) 22, 121, 683, 932
NspV TTCGAA 1 cut(s) 33
PagI TCATGA 1 cut(s) 520
PaqCI CACCTGC 1 cut(s) 1056
PciI ACATGT 1 cut(s) 679
PfeI GAWTC 3 cut(s) 30, 940, 990
PkrI GCNGC 8 cut(s) 109, 160, 163, 166, 504, 610, 625, 998
PleI GAGTC 2 cut(s) 205, 370
PpsI GAGTC 2 cut(s) 205, 370
Ppu21I YACGTR 1 cut(s) 1100
PpuMI RGGWCCY 1 cut(s) 896
PscI ACATGT 1 cut(s) 679
PshAI GACNNNNGTC 1 cut(s) 901
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 896
PspFI CCCAGC 1 cut(s) 280
PspN4I GGNNCC 4 cut(s) 897, 953, 971, 972
PspOMI GGGCCC 1 cut(s) 969
PspPI GGNCC 3 cut(s) 896, 969, 970
PspPPI RGGWCCY 1 cut(s) 896
PstI CTGCAG 1 cut(s) 160
RsaI GTAC 2 cut(s) 217, 1106
RsaNI GTAC 2 cut(s) 216, 1105
RseI CAYNNNNRTG 2 cut(s) 45, 249
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 2 cut(s) 654, 822
SatI GCNGC 8 cut(s) 108, 159, 162, 165, 503, 609, 624, 997
Sau3AI GATC 4 cut(s) 793, 1033, 1037, 1122
Sau96I GGNCC 3 cut(s) 896, 969, 970
SchI GAGTC 2 cut(s) 206, 371
ScrFI CCNGG 1 cut(s) 1020
SduI GDGCHC 2 cut(s) 99, 973
SfaNI GCATC 5 cut(s) 40, 267, 712, 872, 1084
SfcI CTRYAG 3 cut(s) 156, 168, 826
SfuI TTCGAA 1 cut(s) 33
SinI GGWCC 1 cut(s) 896
SmiMI CAYNNNNRTG 2 cut(s) 45, 249
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
SnaBI TACGTA 1 cut(s) 1100
Sse9I AATT 9 cut(s) 188, 345, 506, 634, 719, 812, 871, 1011, 1066
SsiI CCGC 1 cut(s) 165
SspMI CTAG 2 cut(s) 944, 1061
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 1018
TaaI ACNGT 2 cut(s) 388, 491
TaiI ACGT 2 cut(s) 478, 1102
TaqI TCGA 4 cut(s) 33, 90, 589, 1036
TasI AATT 9 cut(s) 188, 345, 506, 634, 719, 812, 871, 1011, 1066
TatI WGTACW 1 cut(s) 1104
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 3 cut(s) 30, 940, 990
Tru1I TTAA 2 cut(s) 654, 822
Tru9I TTAA 2 cut(s) 654, 822
TscAI CASTG 2 cut(s) 207, 730
TseFI GTSAC 4 cut(s) 198, 485, 614, 853
TseI GCWGC 7 cut(s) 107, 158, 161, 502, 608, 623, 996
Tsp45I GTSAC 4 cut(s) 198, 485, 614, 853
TspGWI ACGGA 1 cut(s) 1101
TspRI CASTG 2 cut(s) 207, 730
VpaK11BI GGWCC 1 cut(s) 896
XapI RAATTY 3 cut(s) 188, 634, 871
XbaI TCTAGA 1 cut(s) 943
XceI RCATGY 4 cut(s) 22, 121, 683, 932
XspI CTAG 2 cut(s) 944, 1061
Zsp2I ATGCAT 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.