pycom17g03560

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002335
Physical Location & Seq
Forward (+)
2371634 .. 2376045
4412 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1977 bp
ATGTCATCTCTTCTCGGTTTGCCTGGTCCTGTAAACTCAGTTGGAAGTTTACCTCTTGATTACATAGACATGTCAAAGGAAGATGAAGTTGAAGCTGTGGTTGCCAACACAAAGCAAATTCTCAACCAGTTCCTTCGGAAGTCACAAACAAAGGAGGCAAATTCATTATATTTTAAGCCTTTCAAACCACTGGCCGATTATTCCTCCCTATTGGATCAGATTGAGGATCTAATATCTAATGGAGACTATGAAACTGCAAGGAAGCTGTCTGAAGATCTCAGGGATTTGGCTCTGAAGGGACTTCACTATTTCCAAACTTACGATTGGCTGATGCTCATGACAGTAATTATTCTTGGGTATATTGGTTGGATGACCTATATTCTTCTCCATGTGCTGCAATCTTATACTCCATTGGCAGGGTATATGTTTAGAAAGGAGCAAACAGATCACCTGACAGATAATACCAGAAAGGTACAACTTTGTGGATGCCTTTTCCTGGGACTACTCAGTATAGTACTGTTTATGGAGCGTTCTCCTCCTCTTTACCATGCATACACGTCAATGACAGTATTCCTGTGGACACAAATATTTAGTGAATATCGGTTCATCAAAGCATTATGGAAAGAGTTACATGGAAGAAGAATCAATTACTTTGTGAAAATTCTAGCCACTGGTGCTTTCTCAGTATTCATTCTTGAATTCCTGGTTAATAGCTTTACTGAGAGGAAGCTATACACTTGGTGTTTCCTAGTTTCCGGAGTCGTATCCTTTCTTTATCTCTTAAAATCGATTCCCCGGAGATCTGGCATACCAATCTTTGCCTGTGCTGCATGTTGGTTCTTGTCTTTGTTTACATTGATGCCAGCAGAAATTCCCGATAATAATCGTCTAGTGATTTGGAGTGGAGTTATGATTATTATGATCGGAGTAGCTGCTAGATTGCTAGATCTGCGTACTGAAGGAAAGTATTGGCTAAGTATCTGTAATCATGACAAGAAGAAACCTAAATTTCCCATGCTCTTCCAGTTACAGGCTCTTTTGGTTGGATTGTCATCCGTGATGGTGTCAATATCAACCTCTCACAGAACTGAGAACCAAGAACTACTCGCTTTGCACCAGATCATAAATTGGTCCATCGCTGGTTTCTCGATGGTACTTCCATTATTTTCAGCAAATGGCCTCTTGTCCCGTCTTACTTCCATATTTCTCGGTTTTGCGCCCACTTTTCTTCTATTATCTATCGGATATGAAGCTGTTTTCTACGGTGCTCTGGCTCTTGTACTCATCTCTTGGATACTAGTTGAAAACACACTTATTTACTCAAGTAAGGTGAGAAGGTTGTCAAGTTCCTTTAGTGGTATGGAGGAAAATGTCATACTTGATGGTAGATACTTACAGCTGTCTGATGTGAGAATTCCGTTGATCTTTATGGTTTTATTCAATGTTGCTTTCTTTGGAACGGGAAACTTTGCAAGTATAGCAAGTTTTGAGATTTCATCAGTGTATCGGTTCATTACTGTCTTCAGTCCGTTTCTCATGGCCGCCTTACTTATCTTCAAATTATTCATTCCATTCCTGCTAGTCATATGCGTATTCAGTGCAATAACAAAGCTAAACCGACTTCCACGTTTGGCATGCTATTTTCTCGTTATACTATTTTCGGATGTAATGACCATGCATTTTTTCTTCCTGGTCCGGAATACAGGAAGTTGGATGGAAATTGGTAACAGTATCAGCCATTTCGGAATCATGAGTGCTCAAGTTGTCTTTGTGCTTTTGCTCTTTGCTCTCACAAACATATACACAAACGATATCGACATTGGTTCAGTGGACCGCTCCTCTCGGAAAGCAATGAAAACCAGCAACGGGATTTTCCAAGTGTTCCCCAAAGTTCGGTCGTCCGCAGCGCAATGGGATGGCATCTTACAAAGTTCGATTGAAATTTTGGCACTCTTCTCACATGTCTTGATTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest PigN Sulfatase

Protein Analysis

659

Amino Acids

74.77

Weight (kDa)

8.88

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PigN PF04987 99 - 570 1.2e-127 Phosphatidylinositolglycan class N (PIG-N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1836
AccIII TCCGGA 2 cut(s) 755, 1695
AciI CCGC 3 cut(s) 1542, 1834, 1902
AclWI GGATC 2 cut(s) 222, 234
AcoI YGGCCR 2 cut(s) 192, 1539
AcsI RAATTY 8 cut(s) 117, 160, 660, 698, 870, 1007, 1413, 1941
AcuI CTGAAG 4 cut(s) 291, 314, 978, 1507
AdeI CACNNNGTG 1 cut(s) 741
AfaI GTAC 5 cut(s) 474, 516, 955, 1155, 1281
AfiI CCNNNNNNNGG 5 cut(s) 416, 496, 1030, 1866, 1893
AflIII ACRYGT 3 cut(s) 69, 555, 1960
AgsI TTSAA 7 cut(s) 92, 184, 698, 1304, 1441, 1558, 1940
AhlI ACTAGT 1 cut(s) 1297
AjiI CACGTC 1 cut(s) 558
AjnI CCWGG 4 cut(s) 22, 495, 702, 1689
AluBI AGCT 8 cut(s) 95, 265, 714, 730, 932, 1253, 1399, 1612
AluI AGCT 8 cut(s) 95, 265, 714, 730, 932, 1253, 1399, 1612
Alw21I GWGCWC 2 cut(s) 1270, 1759
Alw26I GTCTC 1 cut(s) 237
AlwI GGATC 2 cut(s) 222, 234
Aor13HI TCCGGA 2 cut(s) 755, 1695
AoxI GGCC 3 cut(s) 192, 1177, 1539
ApeKI GCWGC 4 cut(s) 394, 827, 932, 1904
ApoI RAATTY 8 cut(s) 117, 160, 660, 698, 870, 1007, 1413, 1941
AspLEI GCGC 2 cut(s) 1219, 1909
AspS9I GGNCC 4 cut(s) 26, 1131, 1693, 1831
AsuC2I CCSGG 1 cut(s) 796
AsuHPI GGTGA 2 cut(s) 440, 1342
AvaII GGWCC 4 cut(s) 26, 1131, 1693, 1831
BbsI GAAGAC 1 cut(s) 1513
Bbv12I GWGCWC 2 cut(s) 1270, 1759
BbvI GCAGC 4 cut(s) 381, 814, 919, 1916
BccI CCATC 6 cut(s) 1054, 1142, 1144, 1376, 1708, 1910
BciT130I CCWGG 4 cut(s) 24, 497, 704, 1691
BciVI GTATCC 2 cut(s) 775, 1287
BcnI CCSGG 1 cut(s) 796
BcoDI GTCTC 1 cut(s) 237
BcuI ACTAGT 1 cut(s) 1297
BfaI CTAG 7 cut(s) 665, 749, 890, 936, 944, 1298, 1580
BfuI GTATCC 2 cut(s) 775, 1287
BglII AGATCT 3 cut(s) 274, 800, 946
BisI GCNGC 5 cut(s) 395, 828, 933, 1542, 1905
BlsI GCNGC 5 cut(s) 396, 829, 934, 1543, 1906
BmcAI AGTACT 1 cut(s) 516
Bme1390I CCNGG 5 cut(s) 24, 497, 704, 796, 1691
Bme18I GGWCC 4 cut(s) 26, 1131, 1693, 1831
BmgBI CACGTC 1 cut(s) 558
BmgT120I GGNCC 4 cut(s) 26, 1131, 1693, 1831
BmrFI CCNGG 5 cut(s) 24, 497, 704, 796, 1691
BmsI GCATC 4 cut(s) 321, 476, 849, 1929
BpiI GAAGAC 1 cut(s) 1513
BpuEI CTTGAG 2 cut(s) 1306, 1743
BpuMI CCSGG 1 cut(s) 796
Bsa29I ATCGAT 1 cut(s) 788
BsaBI GATNNNNATC 2 cut(s) 882, 1051
BsaJI CCNNGG 2 cut(s) 496, 794
BsaWI WCCGGW 2 cut(s) 755, 1695
Bsc4I CCNNNNNNNGG 5 cut(s) 416, 496, 1030, 1866, 1893
Bse1I ACTGG 4 cut(s) 127, 195, 676, 1024
Bse3DI GCAATG 2 cut(s) 1857, 1916
Bse8I GATNNNNATC 2 cut(s) 882, 1051
BseAI TCCGGA 2 cut(s) 755, 1695
BseBI CCWGG 4 cut(s) 24, 497, 704, 1691
BseCI ATCGAT 1 cut(s) 788
BseDI CCNNGG 2 cut(s) 496, 794
BseGI GGATG 6 cut(s) 375, 491, 1052, 1669, 1719, 1921
BseJI GATNNNNATC 2 cut(s) 882, 1051
BseLI CCNNNNNNNGG 5 cut(s) 416, 496, 1030, 1866, 1893
BseMI GCAATG 2 cut(s) 1857, 1916
BseMII CTCAG 6 cut(s) 51, 292, 520, 696, 711, 1080
BseNI ACTGG 4 cut(s) 127, 195, 676, 1024
BseRI GAGGAG 3 cut(s) 525, 528, 1828
BseXI GCAGC 4 cut(s) 381, 814, 919, 1916
Bsh1285I CGRYCG 1 cut(s) 1898
BshFI GGCC 3 cut(s) 194, 1179, 1541
BshVI ATCGAT 1 cut(s) 788
BsiEI CGRYCG 1 cut(s) 1898
BsiHKAI GWGCWC 2 cut(s) 1270, 1759
BsiSI CCGG 3 cut(s) 756, 796, 1696
BslFI GGGAC 3 cut(s) 312, 513, 1171
BslI CCNNNNNNNGG 5 cut(s) 416, 496, 1030, 1866, 1893
BsmAI GTCTC 1 cut(s) 237
BsmFI GGGAC 3 cut(s) 312, 513, 1171
BsnI GGCC 3 cut(s) 194, 1179, 1541
Bsp1286I GDGCHC 2 cut(s) 1270, 1759
Bsp13I TCCGGA 2 cut(s) 755, 1695
Bsp143I GATC 9 cut(s) 214, 226, 274, 445, 800, 921, 946, 1119, 1422
BspACI CCGC 3 cut(s) 1542, 1834, 1902
BspANI GGCC 3 cut(s) 194, 1179, 1541
BspCNI CTCAG 6 cut(s) 50, 291, 519, 695, 712, 1081
BspDI ATCGAT 1 cut(s) 788
BspEI TCCGGA 2 cut(s) 755, 1695
BspHI TCATGA 3 cut(s) 336, 988, 1749
BspPI GGATC 2 cut(s) 222, 234
BspQI GCTCTTC 1 cut(s) 1025
BsrBI CCGCTC 1 cut(s) 1836
BsrDI GCAATG 2 cut(s) 1857, 1916
BsrI ACTGG 4 cut(s) 127, 195, 676, 1024
BssECI CCNNGG 2 cut(s) 496, 794
BssMI GATC 9 cut(s) 214, 226, 274, 445, 800, 921, 946, 1119, 1422
Bst2UI CCWGG 4 cut(s) 24, 497, 704, 1691
Bst4CI ACNGT 6 cut(s) 343, 519, 568, 1265, 1519, 1730
Bst6I CTCTTC 3 cut(s) 15, 1025, 1958
BstC8I GCNNGC 2 cut(s) 864, 1636
BstDEI CTNAG 7 cut(s) 37, 278, 506, 682, 720, 974, 1089
BstF5I GGATG 6 cut(s) 375, 491, 1052, 1669, 1719, 1921
BstHHI GCGC 2 cut(s) 1219, 1909
BstKTI GATC 9 cut(s) 217, 229, 277, 448, 803, 924, 949, 1122, 1425
BstMAI GTCTC 1 cut(s) 237
BstMBI GATC 9 cut(s) 214, 226, 274, 445, 800, 921, 946, 1119, 1422
BstMCI CGRYCG 1 cut(s) 1898
BstMWI GCNNNNNNNGC 5 cut(s) 101, 674, 827, 949, 1478
BstNI CCWGG 4 cut(s) 24, 497, 704, 1691
BstNSI RCATGY 4 cut(s) 73, 834, 1638, 1964
BstSCI CCNGG 5 cut(s) 22, 495, 702, 794, 1689
BstV1I GCAGC 4 cut(s) 381, 814, 919, 1916
BstV2I GAAGAC 1 cut(s) 1513
BstX2I RGATCY 4 cut(s) 226, 274, 800, 946
BstYI RGATCY 4 cut(s) 226, 274, 800, 946
Bsu15I ATCGAT 1 cut(s) 788
BsuI GTATCC 2 cut(s) 775, 1287
BsuRI GGCC 3 cut(s) 194, 1179, 1541
BsuTUI ATCGAT 1 cut(s) 788
BtgZI GCGATG 1 cut(s) 1120
BtrI CACGTC 1 cut(s) 558
BtsCI GGATG 6 cut(s) 375, 491, 1052, 1669, 1719, 1921
BtsIMutI CAGTG 5 cut(s) 188, 669, 1506, 1603, 1833
Cac8I GCNNGC 2 cut(s) 864, 1636
CciI TCATGA 3 cut(s) 336, 988, 1749
CfoI GCGC 2 cut(s) 1219, 1909
Cfr13I GGNCC 4 cut(s) 26, 1131, 1693, 1831
ClaI ATCGAT 1 cut(s) 788
Csp6I GTAC 5 cut(s) 473, 515, 954, 1154, 1280
CviQI GTAC 5 cut(s) 473, 515, 954, 1154, 1280
DdeI CTNAG 7 cut(s) 37, 278, 506, 682, 720, 974, 1089
DpnI GATC 9 cut(s) 216, 228, 276, 447, 802, 923, 948, 1121, 1424
DpnII GATC 9 cut(s) 214, 226, 274, 445, 800, 921, 946, 1119, 1422
DraIII CACNNNGTG 1 cut(s) 741
EaeI YGGCCR 2 cut(s) 192, 1539
Eam1104I CTCTTC 3 cut(s) 15, 1025, 1958
EarI CTCTTC 3 cut(s) 15, 1025, 1958
Eco32I GATATC 1 cut(s) 1813
Eco47I GGWCC 4 cut(s) 26, 1131, 1693, 1831
Eco57I CTGAAG 4 cut(s) 291, 314, 978, 1507
EcoRI GAATTC 2 cut(s) 698, 1413
EcoRII CCWGG 4 cut(s) 22, 495, 702, 1689
EcoRV GATATC 1 cut(s) 1813
EcoT22I ATGCAT 2 cut(s) 553, 1680
FaqI GGGAC 3 cut(s) 312, 513, 1171
FauNDI CATATG 1 cut(s) 1586
Fnu4HI GCNGC 5 cut(s) 395, 828, 933, 1542, 1905
FokI GGATG 6 cut(s) 382, 498, 1039, 1676, 1726, 1928
Fsp4HI GCNGC 5 cut(s) 395, 828, 933, 1542, 1905
FspBI CTAG 7 cut(s) 665, 749, 890, 936, 944, 1298, 1580
GlaI GCGC 2 cut(s) 1218, 1908
GluI GCNGC 5 cut(s) 395, 828, 933, 1542, 1905
HaeIII GGCC 3 cut(s) 194, 1179, 1541
HapII CCGG 3 cut(s) 756, 796, 1696
HhaI GCGC 2 cut(s) 1219, 1909
Hin6I GCGC 2 cut(s) 1217, 1907
HinP1I GCGC 2 cut(s) 1217, 1907
HinfI GANTC 4 cut(s) 642, 759, 790, 1746
HpaII CCGG 3 cut(s) 756, 796, 1696
HphI GGTGA 2 cut(s) 440, 1342
Hpy166II GTNNAC 5 cut(s) 34, 50, 579, 852, 1831
Hpy8I GTNNAC 5 cut(s) 34, 50, 579, 852, 1831
HpyAV CCTTC 4 cut(s) 143, 289, 953, 1329
HpyCH4III ACNGT 6 cut(s) 343, 519, 568, 1265, 1519, 1730
HpyCH4IV ACGT 2 cut(s) 557, 1627
HpyCH4V TGCA 8 cut(s) 257, 397, 551, 830, 1114, 1472, 1601, 1678
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 674, 827, 949, 1478
HpyF3I CTNAG 7 cut(s) 37, 278, 506, 682, 720, 974, 1089
HpySE526I ACGT 2 cut(s) 557, 1627
HspAI GCGC 2 cut(s) 1217, 1907
Kpn2I TCCGGA 2 cut(s) 755, 1695
Kzo9I GATC 9 cut(s) 214, 226, 274, 445, 800, 921, 946, 1119, 1422
LguI GCTCTTC 1 cut(s) 1025
LmnI GCTCC 3 cut(s) 436, 526, 1841
Lsp1109I GCAGC 4 cut(s) 381, 814, 919, 1916
LweI GCATC 4 cut(s) 321, 476, 849, 1929
MaeI CTAG 7 cut(s) 665, 749, 890, 936, 944, 1298, 1580
MaeII ACGT 2 cut(s) 557, 1627
MaeIII GTNAC 4 cut(s) 141, 627, 1026, 1724
MalI GATC 9 cut(s) 216, 228, 276, 447, 802, 923, 948, 1121, 1424
MbiI CCGCTC 1 cut(s) 1836
MboI GATC 9 cut(s) 214, 226, 274, 445, 800, 921, 946, 1119, 1422
MflI RGATCY 4 cut(s) 226, 274, 800, 946
MhlI GDGCHC 2 cut(s) 1270, 1759
MlyI GAGTC 1 cut(s) 768
MmeI TCCRAC 4 cut(s) 22, 347, 1024, 1691
Mph1103I ATGCAT 2 cut(s) 553, 1680
MroI TCCGGA 2 cut(s) 755, 1695
MseI TTAA 3 cut(s) 174, 708, 782
MslI CAYNNNNRTG 2 cut(s) 68, 560
MspA1I CMGCKG 1 cut(s) 1399
MspI CCGG 3 cut(s) 756, 796, 1696
MspR9I CCNGG 5 cut(s) 24, 497, 704, 796, 1691
MvaI CCWGG 4 cut(s) 24, 497, 704, 1691
MwoI GCNNNNNNNGC 5 cut(s) 101, 674, 827, 949, 1478
NciI CCSGG 1 cut(s) 796
NdeI CATATG 1 cut(s) 1586
NdeII GATC 9 cut(s) 214, 226, 274, 445, 800, 921, 946, 1119, 1422
NmuCI GTSAC 1 cut(s) 141
NsiI ATGCAT 2 cut(s) 553, 1680
NspI RCATGY 4 cut(s) 73, 834, 1638, 1964
PaeI GCATGC 1 cut(s) 1638
PagI TCATGA 3 cut(s) 336, 988, 1749
PciI ACATGT 2 cut(s) 69, 1960
PciSI GCTCTTC 1 cut(s) 1025
PfeI GAWTC 3 cut(s) 642, 790, 1746
PkrI GCNGC 5 cut(s) 396, 829, 934, 1543, 1906
PleI GAGTC 1 cut(s) 767
PpsI GAGTC 1 cut(s) 767
PscI ACATGT 2 cut(s) 69, 1960
Psp6I CCWGG 4 cut(s) 22, 495, 702, 1689
PspGI CCWGG 4 cut(s) 22, 495, 702, 1689
PspPI GGNCC 4 cut(s) 26, 1131, 1693, 1831
PsuI RGATCY 4 cut(s) 226, 274, 800, 946
PvuII CAGCTG 1 cut(s) 1399
RsaI GTAC 5 cut(s) 474, 516, 955, 1155, 1281
RsaNI GTAC 5 cut(s) 473, 515, 954, 1154, 1280
RseI CAYNNNNRTG 2 cut(s) 68, 560
SapI GCTCTTC 1 cut(s) 1025
SaqAI TTAA 3 cut(s) 174, 708, 782
SatI GCNGC 5 cut(s) 395, 828, 933, 1542, 1905
Sau3AI GATC 9 cut(s) 214, 226, 274, 445, 800, 921, 946, 1119, 1422
Sau96I GGNCC 4 cut(s) 26, 1131, 1693, 1831
ScaI AGTACT 1 cut(s) 516
SchI GAGTC 1 cut(s) 768
ScrFI CCNGG 5 cut(s) 24, 497, 704, 796, 1691
SduI GDGCHC 2 cut(s) 1270, 1759
SfaNI GCATC 4 cut(s) 321, 476, 849, 1929
SinI GGWCC 4 cut(s) 26, 1131, 1693, 1831
SmiMI CAYNNNNRTG 2 cut(s) 68, 560
SmlI CTYRAG 2 cut(s) 1321, 1758
SmoI CTYRAG 2 cut(s) 1321, 1758
SpeI ACTAGT 1 cut(s) 1297
SphI GCATGC 1 cut(s) 1638
SsiI CCGC 3 cut(s) 1542, 1834, 1902
SspI AATATT 1 cut(s) 588
SspMI CTAG 7 cut(s) 665, 749, 890, 936, 944, 1298, 1580
StyD4I CCNGG 5 cut(s) 22, 495, 702, 794, 1689
TaaI ACNGT 6 cut(s) 343, 519, 568, 1265, 1519, 1730
TaiI ACGT 2 cut(s) 560, 1630
TaqI TCGA 4 cut(s) 788, 1148, 1815, 1934
TaqII GACCGA 1 cut(s) 1884
TatI WGTACW 2 cut(s) 514, 1279
TauI GCSGC 1 cut(s) 1544
TfiI GAWTC 3 cut(s) 642, 790, 1746
Tru1I TTAA 3 cut(s) 174, 708, 782
Tru9I TTAA 3 cut(s) 174, 708, 782
TscAI CASTG 5 cut(s) 195, 676, 1506, 1603, 1833
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 4 cut(s) 394, 827, 932, 1904
Tsp45I GTSAC 1 cut(s) 141
TspGWI ACGGA 3 cut(s) 1045, 1407, 1518
TspRI CASTG 5 cut(s) 195, 676, 1506, 1603, 1833
VpaK11BI GGWCC 4 cut(s) 26, 1131, 1693, 1831
XapI RAATTY 8 cut(s) 117, 160, 660, 698, 870, 1007, 1413, 1941
XceI RCATGY 4 cut(s) 73, 834, 1638, 1964
XspI CTAG 7 cut(s) 665, 749, 890, 936, 944, 1298, 1580
ZrmI AGTACT 1 cut(s) 516
Zsp2I ATGCAT 2 cut(s) 553, 1680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.