pycom17g05210

Lamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008382
Physical Location & Seq
Reverse (-)
3603742 .. 3604878
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 513 bp
ATGGAGAAAGAGAGAGCTATAGCGGTGGTGGTGCTTTTGTGCTTGGTGGTGGTGGTGGAACTTCCAGAAGCATCAGCTACGAGATTTATGGTGGGAGGGAAAATGGGTTGGAACAGCAATGTCAACTATACAATTTGGGCTCAGGATAAGCACTTTTACAATGGAGACTGGCTCTTTTTTGTTTATGACAGGAACCAAATGGATGTTCTAGAGGTGAATCAGACCAACTATGTCTCGTGCAATGCCGAGCATCCCGTTCACAACTGGACCACGGGAGCCGGAAGAGACGTGGTTCCACTGAAAGTGACAAGGCACTACTACTTCATCAGTAGCAAGGGGTTCTGCTATGGTGGCATGAAGATCGCTGTTAAGGTTGAAAACATGCCTCCACCTCCCAAAGCTTCCCCGGTGAAGAGCAAGAGCAGTGTCCTAACACCTACCTTTAGATCCCAGTTTGTTTTGCCTGTGGTTTTCGCCATTGGAGCAATGTGGGACACCTTGGTTCGGCTGTGA

Protein Analysis

171

Amino Acids

19.21

Weight (kDa)

8.99

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 37 - 119 1.6e-21 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 23
AclWI GGATC 1 cut(s) 441
AfiI CCNNNNNNNGG 1 cut(s) 504
AgsI TTSAA 1 cut(s) 377
AjiI CACGTC 1 cut(s) 289
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 3 cut(s) 17, 77, 401
AluI AGCT 3 cut(s) 17, 77, 401
Alw26I GTCTC 3 cut(s) 159, 238, 279
AlwI GGATC 1 cut(s) 441
AspS9I GGNCC 1 cut(s) 267
AsuC2I CCSGG 1 cut(s) 407
AsuHPI GGTGA 2 cut(s) 226, 421
AvaII GGWCC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 142
BauI CACGAG 1 cut(s) 235
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BcnI CCSGG 1 cut(s) 407
BcoDI GTCTC 3 cut(s) 159, 238, 279
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 18
Bme1390I CCNGG 1 cut(s) 407
Bme18I GGWCC 1 cut(s) 267
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 1 cut(s) 267
BmiI GGNNCC 3 cut(s) 194, 277, 294
BmrFI CCNGG 1 cut(s) 407
BmrI ACTGGG 1 cut(s) 445
BmsI GCATC 2 cut(s) 80, 259
BmuI ACTGGG 1 cut(s) 445
BplI GAGNNNNNCTC 2 cut(s) 156, 188
Bpu10I CCTNAGC 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 407
BsaJI CCNNGG 3 cut(s) 270, 405, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 504
Bse1I ACTGG 3 cut(s) 173, 269, 451
Bse3DI GCAATG 3 cut(s) 124, 247, 492
BseDI CCNNGG 3 cut(s) 270, 405, 498
BseGI GGATG 2 cut(s) 208, 250
BseLI CCNNNNNNNGG 1 cut(s) 504
BseMI GCAATG 3 cut(s) 124, 247, 492
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 3 cut(s) 173, 269, 451
BsiSI CCGG 2 cut(s) 279, 407
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 1 cut(s) 504
BsmAI GTCTC 3 cut(s) 159, 238, 279
BsmBI CGTCTC 1 cut(s) 279
BsmFI GGGAC 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 2 cut(s) 360, 446
BspACI CCGC 1 cut(s) 23
BspCNI CTCAG 1 cut(s) 154
BspLI GGNNCC 3 cut(s) 194, 277, 294
BspPI GGATC 1 cut(s) 441
BspQI GCTCTTC 1 cut(s) 407
BsrDI GCAATG 3 cut(s) 124, 247, 492
BsrI ACTGG 3 cut(s) 173, 269, 451
BssECI CCNNGG 3 cut(s) 270, 405, 498
BssMI GATC 2 cut(s) 360, 446
BssSI CACGAG 1 cut(s) 235
BssT1I CCWWGG 1 cut(s) 498
Bst2BI CACGAG 1 cut(s) 235
Bst6I CTCTTC 2 cut(s) 277, 407
BstDEI CTNAG 1 cut(s) 141
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 2 cut(s) 208, 250
BstKTI GATC 2 cut(s) 363, 449
BstMAI GTCTC 3 cut(s) 159, 238, 279
BstMBI GATC 2 cut(s) 360, 446
BstMWI GCNNNNNNNGC 2 cut(s) 351, 482
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 1 cut(s) 405
BstSFI CTRYAG 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BtgI CCRYGG 1 cut(s) 270
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 2 cut(s) 208, 250
BtsI GCAGTG 1 cut(s) 430
BtsIMutI CAGTG 2 cut(s) 296, 430
Cfr13I GGNCC 1 cut(s) 267
CviAII CATG 2 cut(s) 355, 382
CviJI RGCY 7 cut(s) 17, 77, 140, 172, 278, 401, 508
CviKI_1 RGCY 7 cut(s) 17, 77, 140, 172, 278, 401, 508
DdeI CTNAG 1 cut(s) 141
DpnI GATC 2 cut(s) 362, 448
DpnII GATC 2 cut(s) 360, 446
Eam1104I CTCTTC 2 cut(s) 277, 407
EarI CTCTTC 2 cut(s) 277, 407
Eco130I CCWWGG 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 142
Eco47I GGWCC 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 498
Esp3I CGTCTC 1 cut(s) 279
FaeI CATG 2 cut(s) 358, 385
FaiI YATR 8 cut(s) 20, 89, 129, 186, 231, 348, 356, 383
FaqI GGGAC 1 cut(s) 506
FatI CATG 2 cut(s) 354, 381
FokI GGATG 2 cut(s) 215, 237
FriOI GRGCYC 1 cut(s) 142
FspBI CTAG 1 cut(s) 209
HapII CCGG 2 cut(s) 279, 407
Hin1II CATG 2 cut(s) 358, 385
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 1 cut(s) 217
HpaII CCGG 2 cut(s) 279, 407
HphI GGTGA 2 cut(s) 226, 421
Hpy166II GTNNAC 2 cut(s) 124, 259
Hpy188I TCNGA 1 cut(s) 222
Hpy188III TCNNGA 3 cut(s) 65, 143, 209
Hpy8I GTNNAC 2 cut(s) 124, 259
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 1 cut(s) 240
HpyF10VI GCNNNNNNNGC 2 cut(s) 351, 482
HpyF3I CTNAG 1 cut(s) 141
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 2 cut(s) 358, 385
Kzo9I GATC 2 cut(s) 360, 446
LguI GCTCTTC 1 cut(s) 407
LmnI GCTCC 2 cut(s) 275, 482
LpnPI CCDG 9 cut(s) 78, 128, 154, 175, 250, 292, 420, 464, 477
LweI GCATC 2 cut(s) 80, 259
MaeI CTAG 1 cut(s) 209
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 304
MalI GATC 2 cut(s) 362, 448
MboI GATC 2 cut(s) 360, 446
MboII GAAGA 3 cut(s) 294, 370, 424
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 1 cut(s) 132
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 4 cut(s) 89, 205, 396, 402
MseI TTAA 1 cut(s) 369
MspI CCGG 2 cut(s) 279, 407
MspR9I CCNGG 1 cut(s) 407
MwoI GCNNNNNNNGC 2 cut(s) 351, 482
NciI CCSGG 1 cut(s) 407
NdeII GATC 2 cut(s) 360, 446
NlaIII CATG 2 cut(s) 358, 385
NlaIV GGNNCC 3 cut(s) 194, 277, 294
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 1 cut(s) 304
NspI RCATGY 1 cut(s) 385
PciSI GCTCTTC 1 cut(s) 407
PfeI GAWTC 1 cut(s) 217
PspN4I GGNNCC 3 cut(s) 194, 277, 294
PspPI GGNCC 1 cut(s) 267
PsuI RGATCY 1 cut(s) 446
SapI GCTCTTC 1 cut(s) 407
SaqAI TTAA 1 cut(s) 369
Sau3AI GATC 2 cut(s) 360, 446
Sau96I GGNCC 1 cut(s) 267
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 1 cut(s) 142
SfaNI GCATC 2 cut(s) 80, 259
SfcI CTRYAG 1 cut(s) 18
SinI GGWCC 1 cut(s) 267
Sse9I AATT 1 cut(s) 132
SsiI CCGC 1 cut(s) 23
SspMI CTAG 1 cut(s) 209
StyD4I CCNGG 1 cut(s) 405
StyI CCWWGG 1 cut(s) 498
TaiI ACGT 1 cut(s) 291
TasI AATT 1 cut(s) 132
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 2 cut(s) 303, 430
TseFI GTSAC 1 cut(s) 304
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 2 cut(s) 313, 371
TspRI CASTG 2 cut(s) 303, 430
VpaK11BI GGWCC 1 cut(s) 267
XbaI TCTAGA 1 cut(s) 208
XceI RCATGY 1 cut(s) 385
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.