pycom17g06220

protein C9orf85 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006795
Physical Location & Seq
Forward (+)
4367353 .. 4369087
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 534 bp
ATGATGTCCGGCTGTCGGGACCAAAACAAATTCGCATGGAAGCCAAAACTCGGCGTCAAAATCAACGAAACAGAGGTCGGAGGCCGGTTCCGGCCGGTGTCGGAGATAACGGGGGTCTGCCAGCGCTGCAAGGAACAGATCGAGTGGAAGCGCCGTTATGGAAAGTACAAGACACTGACGGGGCCCGCCAAGTGCCAACGTTGTACCAAACGCGCAGTCCGTCAGTCCCACCAGAAGCTTTGTGCTGCTTGTTCTAAGGAACAACGTGTTTGTGCGAAATGTTGCTGTCGTGTCGAGCAAATTGTCGGAAAAGACCTTGCGGAAGTGGAGTCTGAGCAGAAGAAGCTTCAAGAGGCGATCAAAAATGCAAGAGAAAGATATAGGAGGAGTCTATTGCGTGCGCGAAAGCTGCAAGCGAGAAAGGAGACAAAGCGGGGGACCTGTTCCCCTCTGCCTCGCTCGAAGAATATGCAGAGGCAAGCAGAGATGATGAGGATGACGACATTGATGATGGTGAAGTGTGGTCTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2039

Protein Analysis

178

Amino Acids

20.55

Weight (kDa)

10.14

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2039 PF10217 7 - 96 1.3e-23 Uncharacterized conserved protein (DUF2039)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 213, 403
AciI CCGC 3 cut(s) 186, 320, 433
AclI AACGTT 1 cut(s) 199
AcoI YGGCCR 1 cut(s) 92
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 167, 205
AfeI AGCGCT 1 cut(s) 125
AfiI CCNNNNNNNGG 2 cut(s) 15, 50
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 1 cut(s) 350
AluBI AGCT 3 cut(s) 238, 346, 409
AluI AGCT 3 cut(s) 238, 346, 409
Alw26I GTCTC 1 cut(s) 419
Aor51HI AGCGCT 1 cut(s) 125
AoxI GGCC 3 cut(s) 82, 92, 182
ApaI GGGCCC 1 cut(s) 186
ApeKI GCWGC 3 cut(s) 126, 245, 409
ApoI RAATTY 1 cut(s) 29
ArsI GACNNNNNNTTYG 4 cut(s) 60, 92, 201, 233
AspLEI GCGC 4 cut(s) 126, 153, 215, 403
AspS9I GGNCC 4 cut(s) 19, 182, 183, 438
AsuHPI GGTGA 1 cut(s) 526
AvaII GGWCC 2 cut(s) 19, 438
BaeGI GKGCMC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 186
BbvI GCAGC 3 cut(s) 113, 232, 396
BccI CCATC 1 cut(s) 505
BceAI ACGGC 1 cut(s) 138
BcgI CGANNNNNNTGC 2 cut(s) 451, 485
BcoDI GTCTC 1 cut(s) 419
BfoI RGCGCY 2 cut(s) 127, 154
BisI GCNGC 3 cut(s) 127, 246, 410
BlsI GCNGC 3 cut(s) 128, 247, 411
Bme18I GGWCC 2 cut(s) 19, 438
BmgT120I GGNCC 4 cut(s) 19, 182, 183, 438
BmiI GGNNCC 5 cut(s) 20, 89, 183, 184, 439
BsaHI GRCGYC 1 cut(s) 54
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 50
Bse118I RCCGGY 2 cut(s) 84, 94
BseGI GGATG 1 cut(s) 501
BseLI CCNNNNNNNGG 2 cut(s) 15, 50
BseMII CTCAG 1 cut(s) 324
BseRI GAGGAG 1 cut(s) 400
BseSI GKGCMC 1 cut(s) 186
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 3 cut(s) 113, 232, 396
Bsh1236I CGCG 2 cut(s) 213, 403
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 3 cut(s) 84, 94, 184
BsiEI CGRYCG 1 cut(s) 95
BsiSI CCGG 4 cut(s) 9, 85, 91, 95
BslFI GGGAC 3 cut(s) 32, 211, 451
BslI CCNNNNNNNGG 2 cut(s) 15, 50
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 3 cut(s) 32, 211, 451
BsnI GGCC 3 cut(s) 84, 94, 184
Bsp120I GGGCCC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 2 cut(s) 138, 357
BspACI CCGC 3 cut(s) 186, 320, 433
BspANI GGCC 3 cut(s) 84, 94, 184
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 2 cut(s) 213, 403
BspLI GGNNCC 5 cut(s) 20, 89, 183, 184, 439
BsrFI RCCGGY 2 cut(s) 84, 94
BssAI RCCGGY 2 cut(s) 84, 94
BssMI GATC 2 cut(s) 138, 357
BssNI GRCGYC 1 cut(s) 54
BstACI GRCGYC 1 cut(s) 54
BstC8I GCNNGC 5 cut(s) 122, 186, 399, 414, 480
BstDEI CTNAG 2 cut(s) 255, 333
BstF5I GGATG 1 cut(s) 501
BstFNI CGCG 2 cut(s) 213, 403
BstH2I RGCGCY 2 cut(s) 127, 154
BstHHI GCGC 4 cut(s) 126, 153, 215, 403
BstKTI GATC 2 cut(s) 141, 360
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 2 cut(s) 138, 357
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 3 cut(s) 126, 343, 409
BstSLI GKGCMC 1 cut(s) 186
BstUI CGCG 2 cut(s) 213, 403
BstV1I GCAGC 3 cut(s) 113, 232, 396
BstZI CGGCCG 1 cut(s) 92
BsuRI GGCC 3 cut(s) 84, 94, 184
BtsCI GGATG 1 cut(s) 501
BtsIMutI CAGTG 1 cut(s) 173
Cac8I GCNNGC 5 cut(s) 122, 186, 399, 414, 480
CfoI GCGC 4 cut(s) 126, 153, 215, 403
Cfr10I RCCGGY 2 cut(s) 84, 94
Cfr13I GGNCC 4 cut(s) 19, 182, 183, 438
CseI GACGC 1 cut(s) 43
Csp6I GTAC 2 cut(s) 166, 204
CviAII CATG 1 cut(s) 36
CviJI RGCY 8 cut(s) 12, 43, 84, 94, 184, 238, 346, 409
CviKI_1 RGCY 8 cut(s) 12, 43, 84, 94, 184, 238, 346, 409
CviQI GTAC 2 cut(s) 166, 204
DdeI CTNAG 2 cut(s) 255, 333
DpnI GATC 2 cut(s) 140, 359
DpnII GATC 2 cut(s) 138, 357
EaeI YGGCCR 1 cut(s) 92
EagI CGGCCG 1 cut(s) 92
EclXI CGGCCG 1 cut(s) 92
Eco24I GRGCYC 1 cut(s) 186
Eco47I GGWCC 2 cut(s) 19, 438
Eco47III AGCGCT 1 cut(s) 125
Eco52I CGGCCG 1 cut(s) 92
EcoO109I RGGNCCY 2 cut(s) 182, 438
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 1 cut(s) 39
FaiI YATR 4 cut(s) 37, 159, 381, 470
FaqI GGGAC 3 cut(s) 32, 211, 451
FatI CATG 1 cut(s) 35
FauI CCCGC 2 cut(s) 193, 426
Fnu4HI GCNGC 3 cut(s) 127, 246, 410
FokI GGATG 1 cut(s) 508
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 3 cut(s) 127, 246, 410
GlaI GCGC 4 cut(s) 125, 152, 214, 402
GluI GCNGC 3 cut(s) 127, 246, 410
HaeII RGCGCY 2 cut(s) 127, 154
HaeIII GGCC 3 cut(s) 84, 94, 184
HapII CCGG 4 cut(s) 9, 85, 91, 95
HgaI GACGC 1 cut(s) 43
HhaI GCGC 4 cut(s) 126, 153, 215, 403
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 1 cut(s) 39
Hin6I GCGC 4 cut(s) 124, 151, 213, 401
HinP1I GCGC 4 cut(s) 124, 151, 213, 401
HindIII AAGCTT 2 cut(s) 236, 344
HinfI GANTC 2 cut(s) 329, 388
HpaII CCGG 4 cut(s) 9, 85, 91, 95
HphI GGTGA 1 cut(s) 526
Hpy188I TCNGA 4 cut(s) 80, 103, 308, 334
Hpy188III TCNNGA 2 cut(s) 17, 350
HpyCH4IV ACGT 2 cut(s) 199, 265
HpyCH4V TGCA 4 cut(s) 129, 368, 412, 472
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 343, 409
HpyF3I CTNAG 2 cut(s) 255, 333
HpySE526I ACGT 2 cut(s) 199, 265
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 1 cut(s) 39
HspAI GCGC 4 cut(s) 124, 151, 213, 401
Kzo9I GATC 2 cut(s) 138, 357
LpnPI CCDG 7 cut(s) 22, 98, 104, 108, 134, 245, 454
Lsp1109I GCAGC 3 cut(s) 113, 232, 396
MaeII ACGT 2 cut(s) 199, 265
MalI GATC 2 cut(s) 140, 359
MboI GATC 2 cut(s) 138, 357
MboII GAAGA 2 cut(s) 352, 475
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 3 cut(s) 29, 300, 529
MlyI GAGTC 2 cut(s) 338, 397
MmeI TCCRAC 3 cut(s) 58, 81, 286
MnlI CCTC 8 cut(s) 67, 74, 346, 378, 459, 465, 468, 486
MseI TTAA 1 cut(s) 532
MspI CCGG 4 cut(s) 9, 85, 91, 95
MvnI CGCG 2 cut(s) 213, 403
MwoI GCNNNNNNNGC 3 cut(s) 126, 343, 409
NdeII GATC 2 cut(s) 138, 357
NlaIII CATG 1 cut(s) 39
NlaIV GGNNCC 5 cut(s) 20, 89, 183, 184, 439
NmeAIII GCCGAG 1 cut(s) 30
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PkrI GCNGC 3 cut(s) 128, 247, 411
PleI GAGTC 2 cut(s) 337, 396
PpsI GAGTC 2 cut(s) 337, 396
PpuMI RGGWCCY 1 cut(s) 438
Psp1406I AACGTT 1 cut(s) 199
Psp5II RGGWCCY 1 cut(s) 438
PspN4I GGNNCC 5 cut(s) 20, 89, 183, 184, 439
PspOMI GGGCCC 1 cut(s) 182
PspPI GGNCC 4 cut(s) 19, 182, 183, 438
PspPPI RGGWCCY 1 cut(s) 438
RsaI GTAC 2 cut(s) 167, 205
RsaNI GTAC 2 cut(s) 166, 204
SaqAI TTAA 1 cut(s) 532
SatI GCNGC 3 cut(s) 127, 246, 410
Sau3AI GATC 2 cut(s) 138, 357
Sau96I GGNCC 4 cut(s) 19, 182, 183, 438
SchI GAGTC 2 cut(s) 338, 397
SduI GDGCHC 1 cut(s) 186
SetI ASST 8 cut(s) 78, 202, 240, 268, 318, 348, 411, 443
SinI GGWCC 2 cut(s) 19, 438
Sse9I AATT 3 cut(s) 29, 300, 529
SsiI CCGC 3 cut(s) 186, 320, 433
TaiI ACGT 2 cut(s) 202, 268
TaqI TCGA 3 cut(s) 141, 294, 461
TasI AATT 3 cut(s) 29, 300, 529
TatI WGTACW 1 cut(s) 165
Tru1I TTAA 1 cut(s) 532
Tru9I TTAA 1 cut(s) 532
TscAI CASTG 1 cut(s) 180
TseI GCWGC 3 cut(s) 126, 245, 409
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 2 cut(s) 19, 438
XapI RAATTY 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.