pycom17g06440

COBRA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002884
Physical Location & Seq
Reverse (-)
4637267 .. 4638427
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g06440.2

Sequence Viewer

Length: 957 bp
ATGAACTGTATGTTTCAGGCGGCTGTTACTGTGAACAACTTCCAAATGTACCGGCACATCATGAGCCCGGGGTGGACATTAGGGTGGACATGGGCCAGAAGAGAAGTGATATGGGCGGCGGTGGGAGCTGAAGCCTCGGAGCAAGGTGACTGCTCCCGGTTCAAAGGGAACGTACCTCATTGTTGCAAGAAAACCCCCACCTTTGTGGACTTGCTTCCTGGTGTTCCTTACAATCAGCAATTCACAAATTGCTGCAAGGGTGGCGTGTTGTCCGCGTGGGGCCAAGACCCCTCGACCGCTGTCTCATCCTTCCAGTTCAGTGTTGGATCAGCCGGCACTACAAACCGTACAGTGAGACTTCCTAGGAACTTCACTCTGCTAGGCCCTGGAACAGGGTACACTTGCGGCAGGGCAAGGATTGTGCCTCCCACAATTTTTTTGACACCTGCCGGACGCCGCAGGACTCAAGCTTTGATGACATGGAACGTGACCTGCTCATATTCACAACTTCGGGCCAGGAAATACCCAAGCTGTTGTGTCTCTCTCTCGTCCTTTTACAATTCCACAATCGTTCATTGCCCTTCTTGCTCTTGTGGTTGCCAGGACAAGAGAAATTGCGTCCAAAGTGATTCCAAAATGCTTAGCGTGGTTGAAGATGACACTAAGCGGAAAGCCAATGCACGGCCGCTACTGCAGTGCACACGCCACATGTGTCCGGTGAGAGTTCACTGGCATGTGATGCTGAATTACAAGGAGTATTGGCGCGTGAAGGTGTCGATTACAAACTTCAACTATCGGATGAACTACACGCATTGGACTCTGGTTATGCAGCATCCTAATTTCAACAATGTCACACAAGTTTTCAGCTTTGATTACAAGCCTCTCATTGCTTACAGCTCCATAAGTGAGTTCAAATCGATTTCCTCTTCCACGTTATCTTCTCGTAGTCATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

319

Amino Acids

36.0

Weight (kDa)

9.56

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 454
Acc36I ACCTGC 2 cut(s) 454, 500
AccII CGCG 2 cut(s) 275, 765
AciI CCGC 9 cut(s) 20, 116, 119, 273, 297, 405, 457, 667, 686
AclWI GGATC 1 cut(s) 334
AcoI YGGCCR 1 cut(s) 683
AcuI CTGAAG 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 454
AfaI GTAC 4 cut(s) 50, 174, 349, 398
AfiI CCNNNNNNNGG 2 cut(s) 392, 681
AflIII ACRYGT 1 cut(s) 708
AgsI TTSAA 5 cut(s) 163, 653, 790, 844, 913
AjnI CCWGG 4 cut(s) 217, 385, 515, 600
AleI CACNNNNGTG 1 cut(s) 203
AluBI AGCT 5 cut(s) 128, 470, 531, 867, 897
AluI AGCT 5 cut(s) 128, 470, 531, 867, 897
Alw21I GWGCWC 1 cut(s) 701
Alw26I GTCTC 3 cut(s) 307, 349, 544
Alw44I GTGCAC 1 cut(s) 697
AlwI GGATC 1 cut(s) 334
Ama87I CYCGRG 1 cut(s) 67
AoxI GGCC 5 cut(s) 93, 280, 382, 513, 683
ApaLI GTGCAC 1 cut(s) 697
ApeKI GCWGC 2 cut(s) 252, 829
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
Asp700I GAANNNNTTC 1 cut(s) 38
AspA2I CCTAGG 1 cut(s) 362
AspLEI GCGC 1 cut(s) 765
AspS9I GGNCC 4 cut(s) 93, 280, 383, 513
AsuC2I CCSGG 3 cut(s) 68, 69, 157
AsuHPI GGTGA 2 cut(s) 158, 730
AvaI CYCGRG 1 cut(s) 67
AvrII CCTAGG 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 701
BbvI GCAGC 2 cut(s) 239, 841
BceAI ACGGC 1 cut(s) 698
BciT130I CCWGG 4 cut(s) 219, 387, 517, 602
BcnI CCSGG 3 cut(s) 68, 69, 157
BcoDI GTCTC 3 cut(s) 307, 349, 544
BfaI CTAG 2 cut(s) 363, 380
BfmI CTRYAG 1 cut(s) 692
BfuAI ACCTGC 2 cut(s) 454, 500
BisI GCNGC 7 cut(s) 21, 117, 253, 406, 457, 686, 830
BlnI CCTAGG 1 cut(s) 362
BlpI GCTNAGC 1 cut(s) 641
BlsI GCNGC 7 cut(s) 22, 118, 254, 407, 458, 687, 831
Bme1390I CCNGG 7 cut(s) 68, 69, 157, 219, 387, 517, 602
BmeT110I CYCGRG 1 cut(s) 67
BmgT120I GGNCC 4 cut(s) 93, 280, 383, 513
BmiI GGNNCC 1 cut(s) 281
BmrFI CCNGG 7 cut(s) 68, 69, 157, 219, 387, 517, 602
BmsI GCATC 2 cut(s) 729, 841
BoxI GACNNNNGTC 1 cut(s) 299
Bpu1102I GCTNAGC 1 cut(s) 641
BpuEI CTTGAG 1 cut(s) 450
BpuMI CCSGG 3 cut(s) 68, 69, 157
Bsa29I ATCGAT 1 cut(s) 917
BsaHI GRCGYC 1 cut(s) 454
BsaJI CCNNGG 5 cut(s) 67, 68, 135, 362, 385
BsaWI WCCGGW 1 cut(s) 715
Bsc4I CCNNNNNNNGG 2 cut(s) 392, 681
Bse118I RCCGGY 2 cut(s) 51, 332
Bse1I ACTGG 2 cut(s) 313, 734
Bse3DI GCAATG 2 cut(s) 574, 885
BseBI CCWGG 4 cut(s) 219, 387, 517, 602
BseCI ATCGAT 1 cut(s) 917
BseDI CCNNGG 5 cut(s) 67, 68, 135, 362, 385
BseGI GGATG 3 cut(s) 305, 804, 832
BseLI CCNNNNNNNGG 2 cut(s) 392, 681
BseMI GCAATG 2 cut(s) 574, 885
BseNI ACTGG 2 cut(s) 313, 734
BseSI GKGCMC 1 cut(s) 701
BseX3I CGGCCG 1 cut(s) 683
BseXI GCAGC 2 cut(s) 239, 841
Bsh1236I CGCG 2 cut(s) 275, 765
Bsh1285I CGRYCG 2 cut(s) 297, 686
BshFI GGCC 5 cut(s) 95, 282, 384, 515, 685
BshVI ATCGAT 1 cut(s) 917
BsiEI CGRYCG 2 cut(s) 297, 686
BsiHKAI GWGCWC 1 cut(s) 701
BsiHKCI CYCGRG 1 cut(s) 67
BsiSI CCGG 6 cut(s) 52, 68, 157, 333, 450, 716
BslI CCNNNNNNNGG 2 cut(s) 392, 681
BsmAI GTCTC 3 cut(s) 307, 349, 544
BsnI GGCC 5 cut(s) 95, 282, 384, 515, 685
BsoBI CYCGRG 1 cut(s) 67
Bsp1286I GDGCHC 2 cut(s) 68, 701
Bsp143I GATC 1 cut(s) 326
Bsp1720I GCTNAGC 1 cut(s) 641
BspACI CCGC 9 cut(s) 20, 116, 119, 273, 297, 405, 457, 667, 686
BspANI GGCC 5 cut(s) 95, 282, 384, 515, 685
BspDI ATCGAT 1 cut(s) 917
BspFNI CGCG 2 cut(s) 275, 765
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 1 cut(s) 281
BspMAI CTGCAG 1 cut(s) 696
BspMI ACCTGC 2 cut(s) 454, 500
BspPI GGATC 1 cut(s) 334
BsrDI GCAATG 2 cut(s) 574, 885
BsrFI RCCGGY 2 cut(s) 51, 332
BsrI ACTGG 2 cut(s) 313, 734
BssAI RCCGGY 2 cut(s) 51, 332
BssECI CCNNGG 5 cut(s) 67, 68, 135, 362, 385
BssMI GATC 1 cut(s) 326
BssNI GRCGYC 1 cut(s) 454
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 4 cut(s) 219, 387, 517, 602
Bst4CI ACNGT 4 cut(s) 8, 31, 347, 352
Bst6I CTCTTC 2 cut(s) 94, 931
BstACI GRCGYC 1 cut(s) 454
BstAPI GCANNNNNTGC 1 cut(s) 739
BstC8I GCNNGC 1 cut(s) 334
BstDEI CTNAG 2 cut(s) 641, 663
BstENI CCTNNNNNAGG 1 cut(s) 390
BstF5I GGATG 3 cut(s) 305, 804, 832
BstFNI CGCG 2 cut(s) 275, 765
BstHHI GCGC 1 cut(s) 765
BstKTI GATC 1 cut(s) 329
BstMAI GTCTC 3 cut(s) 307, 349, 544
BstMBI GATC 1 cut(s) 326
BstMCI CGRYCG 2 cut(s) 297, 686
BstMWI GCNNNNNNNGC 5 cut(s) 125, 261, 585, 691, 739
BstNI CCWGG 4 cut(s) 219, 387, 517, 602
BstNSI RCATGY 2 cut(s) 712, 737
BstPAI GACNNNNGTC 1 cut(s) 299
BstSCI CCNGG 7 cut(s) 66, 67, 155, 217, 385, 515, 600
BstSFI CTRYAG 1 cut(s) 692
BstSLI GKGCMC 1 cut(s) 701
BstUI CGCG 2 cut(s) 275, 765
BstV1I GCAGC 2 cut(s) 239, 841
BstXI CCANNNNNNTGG 1 cut(s) 205
BstZI CGGCCG 1 cut(s) 683
Bsu15I ATCGAT 1 cut(s) 917
BsuRI GGCC 5 cut(s) 95, 282, 384, 515, 685
BsuTUI ATCGAT 1 cut(s) 917
BtsCI GGATG 3 cut(s) 305, 804, 832
BtsI GCAGTG 1 cut(s) 701
BtsIMutI CAGTG 4 cut(s) 325, 357, 701, 727
BveI ACCTGC 2 cut(s) 454, 500
Cac8I GCNNGC 1 cut(s) 334
CciI TCATGA 1 cut(s) 60
CfoI GCGC 1 cut(s) 765
Cfr10I RCCGGY 2 cut(s) 51, 332
Cfr13I GGNCC 4 cut(s) 93, 280, 383, 513
Cfr9I CCCGGG 1 cut(s) 67
ClaI ATCGAT 1 cut(s) 917
CseI GACGC 2 cut(s) 462, 607
Csp6I GTAC 4 cut(s) 49, 173, 348, 397
CviAII CATG 5 cut(s) 61, 90, 480, 709, 734
CviQI GTAC 4 cut(s) 49, 173, 348, 397
DdeI CTNAG 2 cut(s) 641, 663
DpnI GATC 1 cut(s) 328
DpnII GATC 1 cut(s) 326
EaeI YGGCCR 1 cut(s) 683
EagI CGGCCG 1 cut(s) 683
Eam1104I CTCTTC 2 cut(s) 94, 931
EarI CTCTTC 2 cut(s) 94, 931
EclXI CGGCCG 1 cut(s) 683
Eco130I CCWWGG 1 cut(s) 362
Eco24I GRGCYC 1 cut(s) 68
Eco52I CGGCCG 1 cut(s) 683
Eco57I CTGAAG 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 390
EcoO109I RGGNCCY 1 cut(s) 383
EcoRII CCWGG 4 cut(s) 217, 385, 515, 600
EcoT14I CCWWGG 1 cut(s) 362
EcoT38I GRGCYC 1 cut(s) 68
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 5 cut(s) 64, 93, 483, 712, 737
FatI CATG 5 cut(s) 60, 89, 479, 708, 733
Fnu4HI GCNGC 7 cut(s) 21, 117, 253, 406, 457, 686, 830
FokI GGATG 3 cut(s) 292, 811, 819
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 7 cut(s) 21, 117, 253, 406, 457, 686, 830
FspBI CTAG 2 cut(s) 363, 380
GlaI GCGC 1 cut(s) 764
GluI GCNGC 7 cut(s) 21, 117, 253, 406, 457, 686, 830
HaeIII GGCC 5 cut(s) 95, 282, 384, 515, 685
HapII CCGG 6 cut(s) 52, 68, 157, 333, 450, 716
HgaI GACGC 2 cut(s) 462, 607
HhaI GCGC 1 cut(s) 765
Hin1I GRCGYC 1 cut(s) 454
Hin1II CATG 5 cut(s) 64, 93, 483, 712, 737
Hin6I GCGC 1 cut(s) 763
HinP1I GCGC 1 cut(s) 763
HindIII AAGCTT 1 cut(s) 468
HinfI GANTC 3 cut(s) 463, 629, 817
HpaII CCGG 6 cut(s) 52, 68, 157, 333, 450, 716
HphI GGTGA 2 cut(s) 158, 730
Hpy166II GTNNAC 7 cut(s) 34, 75, 87, 208, 399, 699, 727
Hpy188I TCNGA 2 cut(s) 139, 798
Hpy188III TCNNGA 1 cut(s) 61
Hpy8I GTNNAC 7 cut(s) 34, 75, 87, 208, 399, 699, 727
HpyAV CCTTC 3 cut(s) 319, 591, 763
HpyCH4III ACNGT 4 cut(s) 8, 31, 347, 352
HpyCH4IV ACGT 3 cut(s) 171, 486, 932
HpyCH4V TGCA 6 cut(s) 186, 255, 680, 694, 699, 829
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 261, 585, 691, 739
HpyF3I CTNAG 2 cut(s) 641, 663
HpySE526I ACGT 3 cut(s) 171, 486, 932
Hsp92I GRCGYC 1 cut(s) 454
Hsp92II CATG 5 cut(s) 64, 93, 483, 712, 737
HspAI GCGC 1 cut(s) 763
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
Kzo9I GATC 1 cut(s) 326
LmnI GCTCC 4 cut(s) 125, 139, 158, 902
Lsp1109I GCAGC 2 cut(s) 239, 841
LweI GCATC 2 cut(s) 729, 841
MaeI CTAG 2 cut(s) 363, 380
MaeII ACGT 3 cut(s) 171, 486, 932
MaeIII GTNAC 4 cut(s) 25, 146, 487, 850
MalI GATC 1 cut(s) 328
MboI GATC 1 cut(s) 326
MboII GAAGA 4 cut(s) 111, 665, 918, 930
MhlI GDGCHC 2 cut(s) 68, 701
MluCI AATT 7 cut(s) 239, 247, 432, 559, 613, 745, 838
MlyI GAGTC 2 cut(s) 457, 811
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 6 cut(s) 145, 186, 301, 435, 891, 934
MroNI GCCGGC 1 cut(s) 332
MroXI GAANNNNTTC 1 cut(s) 38
MslI CAYNNNNRTG 3 cut(s) 82, 203, 732
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 6 cut(s) 52, 68, 157, 333, 450, 716
MspR9I CCNGG 7 cut(s) 68, 69, 157, 219, 387, 517, 602
MvaI CCWGG 4 cut(s) 219, 387, 517, 602
MvnI CGCG 2 cut(s) 275, 765
MwoI GCNNNNNNNGC 5 cut(s) 125, 261, 585, 691, 739
NaeI GCCGGC 1 cut(s) 334
NciI CCSGG 3 cut(s) 68, 69, 157
NdeII GATC 1 cut(s) 326
NgoMIV GCCGGC 1 cut(s) 332
NlaIII CATG 5 cut(s) 64, 93, 483, 712, 737
NlaIV GGNNCC 1 cut(s) 281
NmuCI GTSAC 3 cut(s) 146, 487, 850
NspI RCATGY 2 cut(s) 712, 737
OliI CACNNNNGTG 1 cut(s) 203
PagI TCATGA 1 cut(s) 60
PaqCI CACCTGC 1 cut(s) 454
PciI ACATGT 1 cut(s) 708
PdiI GCCGGC 1 cut(s) 334
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 629
PkrI GCNGC 7 cut(s) 22, 118, 254, 407, 458, 687, 831
PleI GAGTC 2 cut(s) 457, 811
PpsI GAGTC 2 cut(s) 457, 811
PscI ACATGT 1 cut(s) 708
PshAI GACNNNNGTC 1 cut(s) 299
Psp6I CCWGG 4 cut(s) 217, 385, 515, 600
PspGI CCWGG 4 cut(s) 217, 385, 515, 600
PspN4I GGNNCC 1 cut(s) 281
PspPI GGNCC 4 cut(s) 93, 280, 383, 513
PstI CTGCAG 1 cut(s) 696
RsaI GTAC 4 cut(s) 50, 174, 349, 398
RsaNI GTAC 4 cut(s) 49, 173, 348, 397
RseI CAYNNNNRTG 3 cut(s) 82, 203, 732
SatI GCNGC 7 cut(s) 21, 117, 253, 406, 457, 686, 830
Sau3AI GATC 1 cut(s) 326
Sau96I GGNCC 4 cut(s) 93, 280, 383, 513
SchI GAGTC 2 cut(s) 457, 811
ScrFI CCNGG 7 cut(s) 68, 69, 157, 219, 387, 517, 602
SduI GDGCHC 2 cut(s) 68, 701
SfaNI GCATC 2 cut(s) 729, 841
SfcI CTRYAG 1 cut(s) 692
SmaI CCCGGG 1 cut(s) 69
SmiMI CAYNNNNRTG 3 cut(s) 82, 203, 732
SmlI CTYRAG 1 cut(s) 465
SmoI CTYRAG 1 cut(s) 465
Sse9I AATT 7 cut(s) 239, 247, 432, 559, 613, 745, 838
SsiI CCGC 9 cut(s) 20, 116, 119, 273, 297, 405, 457, 667, 686
SspMI CTAG 2 cut(s) 363, 380
StyD4I CCNGG 7 cut(s) 66, 67, 155, 217, 385, 515, 600
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 4 cut(s) 8, 31, 347, 352
TaiI ACGT 3 cut(s) 174, 489, 935
TaqI TCGA 3 cut(s) 293, 776, 917
TasI AATT 7 cut(s) 239, 247, 432, 559, 613, 745, 838
TauI GCSGC 5 cut(s) 23, 119, 408, 459, 688
TfiI GAWTC 1 cut(s) 629
TscAI CASTG 4 cut(s) 325, 357, 701, 734
TseFI GTSAC 3 cut(s) 146, 487, 850
TseI GCWGC 2 cut(s) 252, 829
Tsp45I GTSAC 3 cut(s) 146, 487, 850
TspDTI ATGAA 3 cut(s) 17, 563, 815
TspMI CCCGGG 1 cut(s) 67
TspRI CASTG 4 cut(s) 325, 357, 701, 734
VneI GTGCAC 1 cut(s) 697
XagI CCTNNNNNAGG 1 cut(s) 390
XceI RCATGY 2 cut(s) 712, 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 320
XmaI CCCGGG 1 cut(s) 67
XmaJI CCTAGG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 38
XspI CTAG 2 cut(s) 363, 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.