pycom17g09410

B-cell receptor-associated protein 31-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0003828
Physical Location & Seq
Forward (+)
7164946 .. 7166187
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g09410.1

Sequence Viewer

Length: 666 bp
ATGTTGCAGCTGCTGTTCACGGTGATTTTCAGCGAAATGCTGCTGATTTTGACCTTGTTGTTTAAGACCCCTCTGAGGAAGCTAGCGATCCTGGCCTTGGATAAGATGAAGCGAGGGAAGGGCCCAGTGATGGTGAAGACGGTCGCTGGGACTGTATTTATTGTTCTGCTGGCCAACGTCTACAATCTGGTGGACATCAAGAACCGAACAAATGAATCTGGTGTGCTCAACCCTACTGACGAGGTTCTCATGTCCATGAAAATGCTCGAAAATTCTCTTATGGGATTTTTCCTGTTCCTTTCACTGATGATGGATAGGTTACACCATTACATAAGAGAACTTCGATTCCTGAGGAAGGCGATGGAGGCTGCGAAAAGACAAAACCAAAGTTCTCAAGACGATAAAAACGGAAGTTCAGAGCAACTTAAGACCTTAGTACAAGAGAATGAGACATTGAGGACAAAGATCAAAACTCTGGAGTCTGAATATGAGACGAAAGCAAAGAAGGCAAAGGCGGCGGGAAGTGAGGTAGAGGCCCTAAGAAAGCAATCTGAAGGACTCCTTATGGAATACGACCGCTTGCTGGCAGACAACCAAAATCTCCGCAGTCAGTTAACATCAATTGAACAGAGCGCATCCCACTCGGATGGAAAGAAGAACACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05165 map04141 map05165
Pfam Domains
Protein Families
Bap31

Protein Analysis

222

Amino Acids

25.15

Weight (kDa)

9.5

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APG6_N PF17675 99 - 215 6.7e-06 Apg6 coiled-coil region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 180
AciI CCGC 4 cut(s) 515, 518, 577, 604
AclWI GGATC 1 cut(s) 82
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 573
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 5 cut(s) 75, 97, 130, 355, 583
AflII CTTAAG 1 cut(s) 425
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 1 cut(s) 626
AjnI CCWGG 1 cut(s) 90
AloI GAACNNNNNNTCC 2 cut(s) 330, 362
AluBI AGCT 2 cut(s) 10, 82
AluI AGCT 2 cut(s) 10, 82
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 443, 485
AlwI GGATC 1 cut(s) 82
AlwNI CAGNNNCTG 1 cut(s) 13
AoxI GGCC 4 cut(s) 93, 121, 171, 534
ApaI GGGCCC 1 cut(s) 125
ApeKI GCWGC 4 cut(s) 7, 10, 40, 368
ApoI RAATTY 1 cut(s) 271
AspLEI GCGC 1 cut(s) 635
AspS9I GGNCC 3 cut(s) 121, 122, 535
AsuHPI GGTGA 2 cut(s) 34, 145
AsuNHI GCTAGC 1 cut(s) 82
AxyI CCTNAGG 1 cut(s) 350
BaeGI GKGCMC 1 cut(s) 125
BalI TGGCCA 1 cut(s) 173
BanII GRGCYC 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 3 cut(s) 19, 27, 355
BccI CCATC 4 cut(s) 124, 304, 355, 641
BcgI CGANNNNNNTGC 2 cut(s) 624, 658
BciT130I CCWGG 1 cut(s) 92
BcoDI GTCTC 2 cut(s) 443, 485
BfaI CTAG 1 cut(s) 83
BfrI CTTAAG 1 cut(s) 425
BisI GCNGC 5 cut(s) 8, 11, 41, 369, 516
BlsI GCNGC 5 cut(s) 9, 12, 42, 370, 517
Bme1390I CCNGG 1 cut(s) 92
BmgT120I GGNCC 3 cut(s) 121, 122, 535
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 92
BmrI ACTGGG 1 cut(s) 119
BmsI GCATC 1 cut(s) 644
BmtI GCTAGC 1 cut(s) 86
BmuI ACTGGG 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 143
BpmI CTGGAG 1 cut(s) 497
BpuEI CTTGAG 1 cut(s) 378
BsaAI YACGTR 1 cut(s) 663
BsaJI CCNNGG 1 cut(s) 96
Bsc4I CCNNNNNNNGG 5 cut(s) 75, 97, 130, 355, 583
Bse1I ACTGG 1 cut(s) 125
Bse21I CCTNAGG 1 cut(s) 350
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 2 cut(s) 635, 652
BseLI CCNNNNNNNGG 5 cut(s) 75, 97, 130, 355, 583
BseMII CTCAG 2 cut(s) 65, 341
BseNI ACTGG 1 cut(s) 125
BseSI GKGCMC 1 cut(s) 125
BseXI GCAGC 3 cut(s) 19, 27, 355
BseYI CCCAGC 1 cut(s) 146
Bsh1285I CGRYCG 2 cut(s) 144, 577
BshFI GGCC 4 cut(s) 95, 123, 173, 536
BsiEI CGRYCG 2 cut(s) 144, 577
BsiHKAI GWGCWC 1 cut(s) 228
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 5 cut(s) 75, 97, 130, 355, 583
BsmAI GTCTC 2 cut(s) 443, 485
BsmBI CGTCTC 1 cut(s) 485
BsmFI GGGAC 1 cut(s) 163
BsnI GGCC 4 cut(s) 95, 123, 173, 536
Bsp120I GGGCCC 1 cut(s) 121
Bsp1286I GDGCHC 2 cut(s) 125, 228
Bsp143I GATC 2 cut(s) 87, 465
BspACI CCGC 4 cut(s) 515, 518, 577, 604
BspANI GGCC 4 cut(s) 95, 123, 173, 536
BspCNI CTCAG 2 cut(s) 66, 342
BspLI GGNNCC 1 cut(s) 123
BspOI GCTAGC 1 cut(s) 86
BspPI GGATC 1 cut(s) 82
BspTI CTTAAG 1 cut(s) 425
BsrI ACTGG 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 2 cut(s) 87, 465
BssT1I CCWWGG 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 3 cut(s) 22, 142, 154
BstAFI CTTAAG 1 cut(s) 425
BstBAI YACGTR 1 cut(s) 663
BstC8I GCNNGC 4 cut(s) 84, 171, 581, 585
BstDEI CTNAG 4 cut(s) 74, 350, 433, 539
BstENI CCTNNNNNAGG 1 cut(s) 353
BstF5I GGATG 2 cut(s) 635, 652
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 2 cut(s) 90, 468
BstMAI GTCTC 2 cut(s) 443, 485
BstMBI GATC 2 cut(s) 87, 465
BstMCI CGRYCG 2 cut(s) 144, 577
BstMWI GCNNNNNNNGC 4 cut(s) 92, 365, 506, 515
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 90
BstSLI GKGCMC 1 cut(s) 125
BstV1I GCAGC 3 cut(s) 19, 27, 355
BstV2I GAAGAC 1 cut(s) 143
BstXI CCANNNNNNTGG 1 cut(s) 647
Bsu36I CCTNAGG 1 cut(s) 350
BsuRI GGCC 4 cut(s) 95, 123, 173, 536
BtgZI GCGATG 1 cut(s) 374
BtsCI GGATG 2 cut(s) 635, 652
BtsIMutI CAGTG 2 cut(s) 132, 302
Cac8I GCNNGC 4 cut(s) 84, 171, 581, 585
CaiI CAGNNNCTG 1 cut(s) 13
CfoI GCGC 1 cut(s) 635
Cfr13I GGNCC 3 cut(s) 121, 122, 535
Csp6I GTAC 1 cut(s) 437
CviAII CATG 2 cut(s) 250, 256
CviJI RGCY 7 cut(s) 10, 82, 95, 123, 173, 368, 536
CviKI_1 RGCY 7 cut(s) 10, 82, 95, 123, 173, 368, 536
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 4 cut(s) 74, 350, 433, 539
DpnI GATC 2 cut(s) 89, 467
DpnII GATC 2 cut(s) 87, 465
EaeI YGGCCR 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 573
Eco81I CCTNAGG 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoO109I RGGNCCY 2 cut(s) 121, 535
EcoRII CCWGG 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 96
EcoT38I GRGCYC 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 96
Esp3I CGTCTC 1 cut(s) 485
FaeI CATG 2 cut(s) 253, 259
FaiI YATR 6 cut(s) 251, 257, 281, 332, 489, 566
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 1 cut(s) 163
FatI CATG 2 cut(s) 249, 255
FauI CCCGC 1 cut(s) 511
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 5 cut(s) 8, 11, 41, 369, 516
FokI GGATG 2 cut(s) 622, 659
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 5 cut(s) 8, 11, 41, 369, 516
FspBI CTAG 1 cut(s) 83
GlaI GCGC 1 cut(s) 634
GluI GCNGC 5 cut(s) 8, 11, 41, 369, 516
GsaI CCCAGC 1 cut(s) 150
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 4 cut(s) 95, 123, 173, 536
HhaI GCGC 1 cut(s) 635
Hin1II CATG 2 cut(s) 253, 259
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HincII GTYRAC 1 cut(s) 615
HindII GTYRAC 1 cut(s) 615
HinfI GANTC 4 cut(s) 215, 345, 479, 558
HpaI GTTAAC 1 cut(s) 615
HphI GGTGA 2 cut(s) 34, 145
Hpy166II GTNNAC 4 cut(s) 18, 181, 193, 615
Hpy188I TCNGA 5 cut(s) 75, 418, 484, 553, 646
Hpy188III TCNNGA 4 cut(s) 199, 349, 395, 476
Hpy8I GTNNAC 4 cut(s) 18, 181, 193, 615
HpyAV CCTTC 4 cut(s) 112, 349, 499, 548
HpyCH4III ACNGT 3 cut(s) 22, 142, 154
HpyCH4IV ACGT 2 cut(s) 177, 662
HpyCH4V TGCA 1 cut(s) 7
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 365, 506, 515
HpyF3I CTNAG 4 cut(s) 74, 350, 433, 539
HpySE526I ACGT 2 cut(s) 177, 662
Hsp92II CATG 2 cut(s) 253, 259
HspAI GCGC 1 cut(s) 633
KspAI GTTAAC 1 cut(s) 615
Kzo9I GATC 2 cut(s) 87, 465
Lsp1109I GCAGC 3 cut(s) 19, 27, 355
LweI GCATC 1 cut(s) 644
MaeI CTAG 1 cut(s) 83
MaeII ACGT 2 cut(s) 177, 662
MaeIII GTNAC 1 cut(s) 318
MalI GATC 2 cut(s) 89, 467
MboI GATC 2 cut(s) 87, 465
MboII GAAGA 1 cut(s) 148
MfeI CAATTG 1 cut(s) 621
MhlI GDGCHC 2 cut(s) 125, 228
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 2 cut(s) 271, 621
MluNI TGGCCA 1 cut(s) 173
MlyI GAGTC 2 cut(s) 488, 552
MnlI CCTC 9 cut(s) 69, 81, 107, 235, 345, 358, 450, 520, 526
Mox20I TGGCCA 1 cut(s) 173
MscI TGGCCA 1 cut(s) 173
MseI TTAA 3 cut(s) 63, 426, 614
MslI CAYNNNNRTG 3 cut(s) 254, 260, 645
Msp20I TGGCCA 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 10
MspCI CTTAAG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 92
MunI CAATTG 1 cut(s) 621
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 4 cut(s) 92, 365, 506, 515
NdeII GATC 2 cut(s) 87, 465
NheI GCTAGC 1 cut(s) 82
NlaIII CATG 2 cut(s) 253, 259
NlaIV GGNNCC 1 cut(s) 123
PfeI GAWTC 2 cut(s) 215, 345
PkrI GCNGC 5 cut(s) 9, 12, 42, 370, 517
PleI GAGTC 2 cut(s) 487, 552
PpsI GAGTC 2 cut(s) 487, 552
Ppu21I YACGTR 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 90
PspFI CCCAGC 1 cut(s) 146
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 123
PspOMI GGGCCC 1 cut(s) 121
PspPI GGNCC 3 cut(s) 121, 122, 535
PsrI GAACNNNNNNTAC 2 cut(s) 147, 179
PstNI CAGNNNCTG 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 10
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
RseI CAYNNNNRTG 3 cut(s) 254, 260, 645
SaqAI TTAA 3 cut(s) 63, 426, 614
SatI GCNGC 5 cut(s) 8, 11, 41, 369, 516
Sau3AI GATC 2 cut(s) 87, 465
Sau96I GGNCC 3 cut(s) 121, 122, 535
SchI GAGTC 2 cut(s) 488, 552
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 2 cut(s) 125, 228
SetI ASST 9 cut(s) 12, 56, 84, 180, 246, 320, 434, 531, 665
SfaNI GCATC 1 cut(s) 644
SmiMI CAYNNNNRTG 3 cut(s) 254, 260, 645
SmlI CTYRAG 2 cut(s) 393, 425
SmoI CTYRAG 2 cut(s) 393, 425
Sse9I AATT 2 cut(s) 271, 621
SsiI CCGC 4 cut(s) 515, 518, 577, 604
SspMI CTAG 1 cut(s) 83
StyD4I CCNGG 1 cut(s) 90
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 3 cut(s) 22, 142, 154
TaiI ACGT 2 cut(s) 180, 665
TaqI TCGA 2 cut(s) 267, 343
TasI AATT 2 cut(s) 271, 621
TatI WGTACW 1 cut(s) 436
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 2 cut(s) 215, 345
Tru1I TTAA 3 cut(s) 63, 426, 614
Tru9I TTAA 3 cut(s) 63, 426, 614
TscAI CASTG 2 cut(s) 132, 309
TseI GCWGC 4 cut(s) 7, 10, 40, 368
TspDTI ATGAA 3 cut(s) 122, 228, 272
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 2 cut(s) 132, 309
Vha464I CTTAAG 1 cut(s) 425
XagI CCTNNNNNAGG 1 cut(s) 353
XapI RAATTY 1 cut(s) 271
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.