RLG00000021124

B-cell receptor-associated protein 31-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003828
Physical Location & Seq
Reverse (-)
72144967 .. 72147012
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021124

Sequence Viewer

Length: 657 bp
ATGATGCAGCTCCTGTACACGGCGATCTTCAGCCAAATGGCGCTGATTCTGACGCTGCTGTTCAAGACGCCTCTGAGGAAGCTGGTGATTCTGGGCCTCGATAAGCTGAAGCGAGGCAAAGGCCCAGTGGTGGTCAAGACTGTGGGAGGTACTGTGGCCGCTGTGCTCTTCGCCAACGTCTACAATCTCAATGAGATCAGGAGCCGCACCAACGAGTCCGGCATGCTCAACCCTACCGACGAGGTCCTCATGTCCATGAAAATGCTCGAGATTTCTCTCATGGGGTTTTTCCTGTTTCTTTCACTGATGATTGATAGATTGCACCATTACATAAGAGAACTTCGATTTCTGAGGAAGGCCATGGAGGCTGCAAAGAAACAAAACCAAACTACTCAAGACGAGAAAAATGAGGGCCACAAGACCCTGGTACAAGAGAATGACACATTGAGGACAAGAATTAAGAATCTGGAATCTGAATATGAGACCAAAGAAAAGAAAGCAAAGGTAGCAGGAGCTGAGGTAGAGGCCCTAAGGAAGCAATCTGAGGGACTCCTCCTGGAATACGATCGCTTGCTGGCAGAGAACCAAAATCTTCGGGACCAGTTGAAATCAATTGATCAGAGCATATCACACTCTGATGACAAAAAAAAGACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05165 map04141 map05165
Pfam Domains
Protein Families
Bap31

Protein Analysis

219

Amino Acids

25.0

Weight (kDa)

9.45

Isoelectric Point (pI)

34.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bap31_Bap29_C PF18035 152 - 199 6.2e-07 Bap31/Bap29 cytoplasmic coiled-coil domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 180
AciI CCGC 2 cut(s) 159, 205
AcoI YGGCCR 1 cut(s) 156
AcuI CTGAAG 2 cut(s) 13, 128
AcyI GRCGYC 1 cut(s) 68
AfaI GTAC 3 cut(s) 17, 151, 429
AfiI CCNNNNNNNGG 2 cut(s) 19, 130
AgsI TTSAA 2 cut(s) 64, 607
AjnI CCWGG 2 cut(s) 423, 555
AluBI AGCT 4 cut(s) 10, 82, 106, 515
AluI AGCT 4 cut(s) 10, 82, 106, 515
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 1 cut(s) 476
AlwNI CAGNNNCTG 2 cut(s) 13, 515
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 6 cut(s) 94, 121, 156, 357, 412, 525
ApeKI GCWGC 3 cut(s) 7, 55, 368
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 6 cut(s) 94, 122, 244, 412, 526, 598
AsuHPI GGTGA 1 cut(s) 97
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 2 cut(s) 244, 598
AxyI CCTNAGG 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 168
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 3 cut(s) 19, 42, 355
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 2 cut(s) 425, 557
BclI TGATCA 1 cut(s) 616
BcoDI GTCTC 1 cut(s) 476
BfoI RGCGCY 1 cut(s) 44
BglI GCCNNNNNGGC 1 cut(s) 365
BisI GCNGC 5 cut(s) 8, 56, 159, 205, 369
BlsI GCNGC 5 cut(s) 9, 57, 160, 206, 370
Bme1390I CCNGG 2 cut(s) 425, 557
Bme18I GGWCC 2 cut(s) 244, 598
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 6 cut(s) 94, 122, 244, 412, 526, 598
BmiI GGNNCC 2 cut(s) 203, 599
BmrFI CCNGG 2 cut(s) 425, 557
BmrI ACTGGG 1 cut(s) 119
BmuI ACTGGG 1 cut(s) 119
Bpu10I CCTNAGC 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 378
BsaHI GRCGYC 1 cut(s) 68
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 2 cut(s) 360, 423
BsaXI ACNNNNNCTCC 4 cut(s) 138, 168, 504, 534
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 130
Bse1I ACTGG 2 cut(s) 125, 601
Bse21I CCTNAGG 1 cut(s) 530
BseBI CCWGG 2 cut(s) 425, 557
BseDI CCNNGG 2 cut(s) 360, 423
BseLI CCNNNNNNNGG 2 cut(s) 19, 130
BseMII CTCAG 4 cut(s) 65, 341, 507, 534
BseNI ACTGG 2 cut(s) 125, 601
BseRI GAGGAG 1 cut(s) 542
BseXI GCAGC 3 cut(s) 19, 42, 355
Bsh1285I CGRYCG 1 cut(s) 568
BshFI GGCC 6 cut(s) 96, 123, 158, 359, 414, 527
BsiEI CGRYCG 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 1 cut(s) 219
BslFI GGGAC 2 cut(s) 561, 611
BslI CCNNNNNNNGG 2 cut(s) 19, 130
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 2 cut(s) 561, 611
BsnI GGCC 6 cut(s) 96, 123, 158, 359, 414, 527
Bso31I GGTCTC 1 cut(s) 476
BsoBI CYCGRG 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 168
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 4 cut(s) 24, 195, 565, 616
Bsp19I CCATGG 1 cut(s) 360
BspACI CCGC 2 cut(s) 159, 205
BspANI GGCC 6 cut(s) 96, 123, 158, 359, 414, 527
BspCNI CTCAG 4 cut(s) 66, 342, 508, 535
BspLI GGNNCC 2 cut(s) 203, 599
BspQI GCTCTTC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 476
BsrGI TGTACA 1 cut(s) 15
BsrI ACTGG 2 cut(s) 125, 601
BssECI CCNNGG 2 cut(s) 360, 423
BssMI GATC 4 cut(s) 24, 195, 565, 616
BssNI GRCGYC 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 360
Bst2UI CCWGG 2 cut(s) 425, 557
Bst4CI ACNGT 2 cut(s) 142, 154
Bst6I CTCTTC 1 cut(s) 173
BstACI GRCGYC 1 cut(s) 68
BstAUI TGTACA 1 cut(s) 15
BstC8I GCNNGC 3 cut(s) 224, 572, 576
BstDEI CTNAG 5 cut(s) 74, 350, 516, 530, 543
BstDSI CCRYGG 1 cut(s) 360
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 4 cut(s) 27, 198, 568, 619
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 4 cut(s) 24, 195, 565, 616
BstMCI CGRYCG 1 cut(s) 568
BstMWI GCNNNNNNNGC 2 cut(s) 365, 506
BstNI CCWGG 2 cut(s) 425, 557
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 423, 555
BstV1I GCAGC 3 cut(s) 19, 42, 355
Bsu36I CCTNAGG 1 cut(s) 530
BsuRI GGCC 6 cut(s) 96, 123, 158, 359, 414, 527
BtgI CCRYGG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 132, 302
Cac8I GCNNGC 3 cut(s) 224, 572, 576
CaiI CAGNNNCTG 2 cut(s) 13, 515
CfoI GCGC 1 cut(s) 43
Cfr13I GGNCC 6 cut(s) 94, 122, 244, 412, 526, 598
CseI GACGC 2 cut(s) 61, 76
Csp6I GTAC 3 cut(s) 16, 150, 428
CviAII CATG 5 cut(s) 223, 250, 256, 280, 361
CviQI GTAC 3 cut(s) 16, 150, 428
DdeI CTNAG 5 cut(s) 74, 350, 516, 530, 543
DpnI GATC 4 cut(s) 26, 197, 567, 618
DpnII GATC 4 cut(s) 24, 195, 565, 616
EaeI YGGCCR 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 360
Eco31I GGTCTC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 244, 598
Eco57I CTGAAG 2 cut(s) 13, 128
Eco81I CCTNAGG 1 cut(s) 530
Eco88I CYCGRG 1 cut(s) 266
EcoO109I RGGNCCY 2 cut(s) 244, 526
EcoRII CCWGG 2 cut(s) 423, 555
EcoT14I CCWWGG 1 cut(s) 360
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 5 cut(s) 226, 253, 259, 283, 364
FaiI YATR 8 cut(s) 224, 251, 257, 281, 332, 362, 480, 626
FaqI GGGAC 2 cut(s) 561, 611
FatI CATG 5 cut(s) 222, 249, 255, 279, 360
FbaI TGATCA 1 cut(s) 616
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 5 cut(s) 8, 56, 159, 205, 369
Fsp4HI GCNGC 5 cut(s) 8, 56, 159, 205, 369
GlaI GCGC 1 cut(s) 42
GluI GCNGC 5 cut(s) 8, 56, 159, 205, 369
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 6 cut(s) 96, 123, 158, 359, 414, 527
HapII CCGG 1 cut(s) 219
HgaI GACGC 2 cut(s) 61, 76
HhaI GCGC 1 cut(s) 43
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 5 cut(s) 226, 253, 259, 283, 364
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HinfI GANTC 6 cut(s) 46, 88, 215, 463, 470, 549
HpaII CCGG 1 cut(s) 219
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 2 cut(s) 18, 181
Hpy188I TCNGA 7 cut(s) 51, 75, 351, 475, 544, 621, 637
Hpy188III TCNNGA 7 cut(s) 64, 136, 199, 268, 395, 467, 596
Hpy8I GTNNAC 2 cut(s) 18, 181
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 1 cut(s) 349
HpyCH4III ACNGT 2 cut(s) 142, 154
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 3 cut(s) 7, 322, 371
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 506
HpyF3I CTNAG 5 cut(s) 74, 350, 516, 530, 543
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 5 cut(s) 226, 253, 259, 283, 364
HspAI GCGC 1 cut(s) 41
Ksp22I TGATCA 1 cut(s) 616
Kzo9I GATC 4 cut(s) 24, 195, 565, 616
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 3 cut(s) 15, 201, 512
Lsp1109I GCAGC 3 cut(s) 19, 42, 355
MaeII ACGT 1 cut(s) 177
MalI GATC 4 cut(s) 26, 197, 567, 618
MboI GATC 4 cut(s) 24, 195, 565, 616
MboII GAAGA 3 cut(s) 19, 160, 584
MfeI CAATTG 1 cut(s) 612
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 2 cut(s) 456, 612
MlyI GAGTC 2 cut(s) 224, 543
MseI TTAA 2 cut(s) 459, 655
MslI CAYNNNNRTG 3 cut(s) 254, 260, 636
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 1 cut(s) 219
MspR9I CCNGG 2 cut(s) 425, 557
MunI CAATTG 1 cut(s) 612
MvaI CCWGG 2 cut(s) 425, 557
MwoI GCNNNNNNNGC 2 cut(s) 365, 506
NcoI CCATGG 1 cut(s) 360
NdeII GATC 4 cut(s) 24, 195, 565, 616
NlaIII CATG 5 cut(s) 226, 253, 259, 283, 364
NlaIV GGNNCC 2 cut(s) 203, 599
NspI RCATGY 1 cut(s) 226
PaeI GCATGC 1 cut(s) 226
PaeR7I CTCGAG 1 cut(s) 266
PciSI GCTCTTC 1 cut(s) 173
PfeI GAWTC 4 cut(s) 46, 88, 463, 470
PflFI GACNNNGTC 1 cut(s) 242
PfoI TCCNGGA 1 cut(s) 555
PkrI GCNGC 5 cut(s) 9, 57, 160, 206, 370
Ple19I CGATCG 1 cut(s) 568
PleI GAGTC 2 cut(s) 223, 543
PpsI GAGTC 2 cut(s) 223, 543
PpuMI RGGWCCY 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 244
Psp6I CCWGG 2 cut(s) 423, 555
PspGI CCWGG 2 cut(s) 423, 555
PspN4I GGNNCC 2 cut(s) 203, 599
PspPI GGNCC 6 cut(s) 94, 122, 244, 412, 526, 598
PspPPI RGGWCCY 1 cut(s) 244
PstNI CAGNNNCTG 2 cut(s) 13, 515
PsyI GACNNNGTC 1 cut(s) 242
PvuI CGATCG 1 cut(s) 568
RsaI GTAC 3 cut(s) 17, 151, 429
RsaNI GTAC 3 cut(s) 16, 150, 428
RseI CAYNNNNRTG 3 cut(s) 254, 260, 636
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 2 cut(s) 459, 655
SatI GCNGC 5 cut(s) 8, 56, 159, 205, 369
Sau3AI GATC 4 cut(s) 24, 195, 565, 616
Sau96I GGNCC 6 cut(s) 94, 122, 244, 412, 526, 598
SchI GAGTC 2 cut(s) 224, 543
ScrFI CCNGG 2 cut(s) 425, 557
SduI GDGCHC 1 cut(s) 168
SetI ASST 9 cut(s) 12, 84, 108, 151, 180, 246, 507, 517, 522
Sfr274I CTCGAG 1 cut(s) 266
SinI GGWCC 2 cut(s) 244, 598
SlaI CTCGAG 1 cut(s) 266
SmiMI CAYNNNNRTG 3 cut(s) 254, 260, 636
SmlI CTYRAG 2 cut(s) 266, 393
SmoI CTYRAG 2 cut(s) 266, 393
SphI GCATGC 1 cut(s) 226
Sse9I AATT 2 cut(s) 456, 612
SsiI CCGC 2 cut(s) 159, 205
StyD4I CCNGG 2 cut(s) 423, 555
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 2 cut(s) 142, 154
TaiI ACGT 1 cut(s) 180
TaqI TCGA 3 cut(s) 99, 267, 343
TasI AATT 2 cut(s) 456, 612
TatI WGTACW 1 cut(s) 15
TauI GCSGC 2 cut(s) 161, 207
TfiI GAWTC 4 cut(s) 46, 88, 463, 470
Tru1I TTAA 2 cut(s) 459, 655
Tru9I TTAA 2 cut(s) 459, 655
TscAI CASTG 2 cut(s) 132, 309
TseI GCWGC 3 cut(s) 7, 55, 368
TspDTI ATGAA 1 cut(s) 272
TspRI CASTG 2 cut(s) 132, 309
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 2 cut(s) 244, 598
XceI RCATGY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.