pycom17g14440

CCAAT enhancer-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008077
Physical Location & Seq
Reverse (-)
11981742 .. 11988849
7108 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3066 bp
ATGGCGGATTCAAAATCAAAGAAATCCGCAAGCAACCCAGAAGACATAGAGCACCTCCGGTCCGACGTAGCCTCCTTCGCTTCCTCTCTCGGCCTCGCCTCCTCTCTCCCTTCCTCCGGCTTCAATGATGTCGATTTTCGCAATCCCGGTCCCAAAAAACCCCACAAGAAGTCCAAACCAGCTCCAATCCAGAACCCCACCAAAACCCAGAAGCCAAACAAGCCGAATTTCAAGCCCAATGAAAACCAGAAGCCGAAACTCACACTTTCTTCGCTGGAAGAAAACAGCAACAAGGACAAAGCGAGGAACTTTGAGAAGTTCAAGAACCTCCCGAAGCTGCCATTGATGTCGGCAAACAACTTGGGAGTGTGGTATGAGGAAGCTGAGGAGTTCGAGGGTAAAGTGCTTGCGAGTGGGAAGAAAGTGGAGGTGAAGAATGCGGAGGAATGGAATAGTGTGGTGGCAAAGAAGAGAGAGCTTGGAGAGAGGTTAATGGCGCAGTACGTAGCGGACTACGAGTCTTCGAAGGGGAAGAGTGGGGATATCAAGCTGTTGCTTACTACGCAGAGGTCAGGGACTGCTTCTGATAAGATATCGGCGTTTTCGGTGTTGGTTGGGGATAATCCCATTGCCAATATGAGGTCCCTTGATGCACTTATAGGAATGGTGACATCAAAAGTGGGAAAGCGATATGCATTTGCAGGGTTTGAAGCATTGAGAGAATTGTTTCTTACAAGTTTGTTACCTGATCGTAAGCTTAAGAACCTCTTACAGCGGCCATTAAATCATGTTCCTGAGACAAAAGATGGTTATTCTCTTCTGCTTCTATGGTATTGGGAGGAGTCCTTGAAACAAAGGTATGAACGCTATGTTTTTGCTCTTGAGGAAGCATCAAAAGATATGTTACCTGAACTAAAAAACAAGGCATTGAAGACTATATATGTGCTGCTAAAGAATAAATCGGAGCAGGAGCGCAGATTACTGTCTGCACTAGTTAACAAACTGGGAGATCCTAAAAATAAAGGCGCTTCTGACGCTGATTTTCACTTATCAAACCTTTTGTCTGATCATCCAAACATGAAGGCAGTGGTGATTGATGAGGTGGACTCTTTTCTCTTTCGCCCACGTTTGACTCCACAAGCAAAATATCATGCAGTTAACTTTTTGAGCCAAATGCGTCTAAGTCATAGAGGAGATGGACCAAAGGTTGCAAAACATTTAGTCGATGTTTATTTTTCACTTTTCAAGGTACTTATTAATGAAGTTACTGCTGGTGAAAAGATGAATAAAAAAGGGAAAGCAGGGTCTAAAAAACCACTCAGTTCTAATGAGGATAAGACTTCATCGGATTCTCATGTTGAATTGGATTCACGCCTTCTATCAGCTCTACTGGTGGGAGTCAATAGAGCATTTCCCTTTGTCTCCAGCAATGAGGCTGATGACATTGTTGAGGTCCAAACACCAATGCTTTTCCAGCTGGTTCATTCTAAGAATTTTAACGTAGGAGTTCAAGCATTAATGCTTCTCGATAAGATCTCGTCTAAGAATCAAATCGTCAGTGATAGGTTTTACAGAGCCTTGTACTCAAAACTACTACTTCCTGCTGCCATGAATACTTCTAAGGCTGAAATGTTTATTGGACTTATTCTGAGGGCAATGAAAAATGATGTGAACTTGAAGCGTGCAGCTGCTTTCGCAAAGCGTGTATTGCAGGTTGCGCTTCAGCAGCCACCTCAATATGCATGTGGATGTCTCTTCCTTCTCTCAGAAGTTCTTAAAGCAAGACCTCTGTTATGGAACATGGTGCTTCAGAATGAGTCAGTTGATGATGAACTTGAGCATTTTGAAGATGTTCGAGAAGAAACTGATGATAAACCTACCCCTGTGCCAGAGAAACAAGAACTTGATGTTGAGCTTGTGCACAGCAATGATGCTGCCGATTCTGACCATGATTCTTCAGAAGATGACGAGGAGTCCGCTGCCACTTATTCTGAAGATGAAGGTTCTGATGAAGCAGAGGAGTTTCTTGTGACAAAAGGTCAAAATGATCCCAAACCAGCCCTTGCTGGTGGGCAACAACCACAAGCATCATCTGAGAGCCCTCGCTTGCCTGGAGGCTATGATCCACGACGCAGAGAGCCCTCTTATTGTAATGCTGATCGTGTGAGCTGGTGGGAGCTGACAGTACTTTCTTCACATGTGCACCCTTCAGTTTCTACCATGGCAAAGACCCTACTCTCGGGGGCGAACATTGTCTACAATGGAAACCCATTGAATGACCTATCACTTGCTGCTTTTCTGGACAAGTTTATGGAGAAGAAACCAAAGCAAAGCGCATGGCATGGCGGTTCACAAATTGAACCTGCGAAAAAGCTTGACATGGCCAACCAATTAATTGGACCCGAGATTCTCTCATTGGCCGAGGAAGATGTAGCCCCGGAAGATCTTGTCTTCCACAAGTTCTATATGAACAAAATGAATTCTTCAAAGAAACCAAAGAAGAAAAAGAAGAAGAAATCAACTGAAGATGAAGGCGCTGCAGGTTTGTTTGACGTGGATGGGGGTGGCGGTGATGATAGCGATAACGAAGAGATTGACAATATGCTGGATTCTGCTGGTGTATCTATCGAAGCAGACGGTGACTATGATTATGATGATCTGGACCAAGTTGCCAATGAAGATGATGAAGACTTGGTTGCTAATGTCAGTGATGCTGAGCTGGACCTTCCCTCGGACAGTGGGGAGGGAGAAGACTTTGATGCCATTGCGGACGATGGCCTGAGTGATGATGATGCTATTGATATAGGAGATGCAGACGACGGTGACATTGACATAGGTGATGCAGACGATGACATTGACATAGGGGATGCAGATGACAGCTTTGACGAGGATGAGGACGAGAAGAATGTTAAAAGCAAAACTAAACGGAAGCGAGAGAAGTCTGGAGCATCGCCGTTTGCAAGTCTTGAAGAATATGAGCATCTGTTGAATGATAATGTTCCTGCTGAAAAGGAATGTAGAAAGAAGAAGCCAAAGTCACGAAAGAAGAGAAAGTCATTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBF

Protein Analysis

1022

Amino Acids

113.41

Weight (kDa)

5.06

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBF PF03914 503 - 742 1.3e-43 CBF/Mak21 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1702, 2371, 2531
AccI GTMKAC 1 cut(s) 2256
AciI CCGC 9 cut(s) 5, 27, 440, 509, 775, 1977, 2346, 2568, 2768
AclWI GGATC 3 cut(s) 1004, 2042, 2117
AcoI YGGCCR 3 cut(s) 776, 2382, 2418
AcsI RAATTY 3 cut(s) 226, 1492, 2479
AcuI CTGAAG 6 cut(s) 1706, 1793, 1941, 2013, 2193, 2544
AfaI GTAC 4 cut(s) 503, 1251, 1583, 2187
AfiI CCNNNNNNNGG 4 cut(s) 116, 639, 2239, 2731
AflII CTTAAG 1 cut(s) 758
AflIII ACRYGT 1 cut(s) 2197
AhdI GACNNNNNGTC 3 cut(s) 517, 1972, 2037
AhlI ACTAGT 1 cut(s) 991
AjiI CACGTC 1 cut(s) 2554
AjnI CCWGG 1 cut(s) 2110
AjuI GAANNNNNNNTTGG 2 cut(s) 1620, 1652
Alw21I GWGCWC 3 cut(s) 54, 1923, 2205
Alw26I GTCTC 3 cut(s) 791, 1426, 1757
Alw44I GTGCAC 2 cut(s) 1919, 2201
AlwI GGATC 3 cut(s) 1004, 2042, 2117
AlwNI CAGNNNCTG 1 cut(s) 578
Ama87I CYCGRG 2 cut(s) 2239, 2402
AoxI GGCC 5 cut(s) 91, 776, 2382, 2418, 2776
ApaLI GTGCAC 2 cut(s) 1919, 2201
ApoI RAATTY 3 cut(s) 226, 1492, 2479
ArsI GACNNNNNNTTYG 2 cut(s) 1046, 1078
AseI ATTAAT 3 cut(s) 1257, 1517, 2393
Asp700I GAANNNNTTC 2 cut(s) 726, 1851
AspLEI GCGC 6 cut(s) 499, 975, 1028, 1720, 2336, 2537
AspS9I GGNCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2662, 2722
AsuC2I CCSGG 2 cut(s) 147, 2438
AsuHPI GGTGA 8 cut(s) 442, 679, 1102, 1286, 2582, 2651, 2834, 2849
AsuII TTCGAA 1 cut(s) 524
AvaI CYCGRG 2 cut(s) 2239, 2402
AvaII GGWCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2662, 2722
BaeGI GKGCMC 2 cut(s) 1923, 2205
BalI TGGCCA 1 cut(s) 2384
BanII GRGCYC 2 cut(s) 2102, 2142
BbsI GAAGAC 6 cut(s) 48, 513, 938, 2443, 2694, 2757
Bbv12I GWGCWC 3 cut(s) 54, 1923, 2205
BbvCI CCTCAGC 1 cut(s) 384
BccI CCATC 4 cut(s) 800, 1190, 2552, 2768
BceAI ACGGC 1 cut(s) 2938
BciT130I CCWGG 1 cut(s) 2112
BclI TGATCA 1 cut(s) 1066
BcnI CCSGG 2 cut(s) 147, 2438
BcoDI GTCTC 3 cut(s) 791, 1426, 1757
BcuI ACTAGT 1 cut(s) 991
BfaI CTAG 1 cut(s) 992
BfmI CTRYAG 1 cut(s) 2538
BfoI RGCGCY 2 cut(s) 1029, 2538
BfrI CTTAAG 1 cut(s) 758
BfuAI ACCTGC 3 cut(s) 1702, 2371, 2531
BglII AGATCT 2 cut(s) 1533, 2443
BlpI GCTNAGC 1 cut(s) 2715
BmcAI AGTACT 1 cut(s) 2187
Bme1390I CCNGG 3 cut(s) 147, 2112, 2438
Bme18I GGWCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2662, 2722
BmeRI GACNNNNNGTC 3 cut(s) 517, 1972, 2037
BmeT110I CYCGRG 2 cut(s) 2239, 2402
BmgBI CACGTC 1 cut(s) 2554
BmgT120I GGNCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2662, 2722
BmiI GGNNCC 3 cut(s) 151, 644, 2401
BmrFI CCNGG 3 cut(s) 147, 2112, 2438
BmrI ACTGGG 1 cut(s) 1013
BmuI ACTGGG 1 cut(s) 1013
BpiI GAAGAC 6 cut(s) 48, 513, 938, 2443, 2694, 2757
BplI GAGNNNNNCTC 4 cut(s) 1091, 1123, 2396, 2428
BpmI CTGGAG 3 cut(s) 1408, 2133, 2964
Bpu10I CCTNAGC 1 cut(s) 384
Bpu1102I GCTNAGC 1 cut(s) 2715
Bpu14I TTCGAA 1 cut(s) 524
BpuEI CTTGAG 2 cut(s) 902, 1856
BpuMI CCSGG 2 cut(s) 147, 2438
BsaAI YACGTR 1 cut(s) 505
BsaJI CCNNGG 4 cut(s) 2220, 2421, 2436, 2730
BsaWI WCCGGW 1 cut(s) 57
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 4 cut(s) 116, 639, 2239, 2731
Bse1I ACTGG 2 cut(s) 1008, 1395
Bse3DI GCAATG 5 cut(s) 627, 1435, 1662, 1933, 2763
BseBI CCWGG 1 cut(s) 2112
BseDI CCNNGG 4 cut(s) 2220, 2421, 2436, 2730
BseGI GGATG 5 cut(s) 1069, 1754, 2563, 2872, 2896
BseLI CCNNNNNNNGG 4 cut(s) 116, 639, 2239, 2731
BseMI GCAATG 5 cut(s) 627, 1435, 1662, 1933, 2763
BseMII CTCAG 8 cut(s) 375, 786, 1333, 1640, 1779, 2085, 2706, 2771
BseNI ACTGG 2 cut(s) 1008, 1395
BseRI GAGGAG 6 cut(s) 91, 401, 854, 1206, 1985, 2033
BseSI GKGCMC 2 cut(s) 1923, 2205
BsgI GTGCAG 2 cut(s) 972, 1704
BshFI GGCC 5 cut(s) 93, 778, 2384, 2420, 2778
BsiHKAI GWGCWC 3 cut(s) 54, 1923, 2205
BsiHKCI CYCGRG 2 cut(s) 2239, 2402
BsiSI CCGG 4 cut(s) 58, 117, 147, 2438
BslFI GGGAC 3 cut(s) 135, 589, 628
BslI CCNNNNNNNGG 4 cut(s) 116, 639, 2239, 2731
BsmAI GTCTC 3 cut(s) 791, 1426, 1757
BsmFI GGGAC 3 cut(s) 135, 589, 628
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 5 cut(s) 93, 778, 2384, 2420, 2778
BsoBI CYCGRG 2 cut(s) 2239, 2402
Bsp119I TTCGAA 1 cut(s) 524
Bsp1286I GDGCHC 5 cut(s) 54, 1923, 2102, 2142, 2205
Bsp143I GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2656
Bsp1720I GCTNAGC 1 cut(s) 2715
Bsp19I CCATGG 1 cut(s) 2220
BspACI CCGC 9 cut(s) 5, 27, 440, 509, 775, 1977, 2346, 2568, 2768
BspANI GGCC 5 cut(s) 93, 778, 2384, 2420, 2778
BspCNI CTCAG 8 cut(s) 376, 787, 1332, 1641, 1778, 2086, 2707, 2772
BspLI GGNNCC 3 cut(s) 151, 644, 2401
BspMAI CTGCAG 1 cut(s) 2542
BspMI ACCTGC 3 cut(s) 1702, 2371, 2531
BspPI GGATC 3 cut(s) 1004, 2042, 2117
BspT104I TTCGAA 1 cut(s) 524
BspTI CTTAAG 1 cut(s) 758
BsrDI GCAATG 5 cut(s) 627, 1435, 1662, 1933, 2763
BsrI ACTGG 2 cut(s) 1008, 1395
BssECI CCNNGG 4 cut(s) 2220, 2421, 2436, 2730
BssMI GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2656
BssT1I CCWWGG 1 cut(s) 2220
Bst2UI CCWGG 1 cut(s) 2112
Bst4CI ACNGT 5 cut(s) 984, 2185, 2639, 2738, 2822
Bst6I CTCTTC 6 cut(s) 464, 527, 822, 1760, 2583, 3041
BstAFI CTTAAG 1 cut(s) 758
BstBAI YACGTR 1 cut(s) 505
BstBI TTCGAA 1 cut(s) 524
BstC8I GCNNGC 4 cut(s) 31, 408, 1683, 2108
BstDSI CCRYGG 1 cut(s) 2220
BstF5I GGATG 5 cut(s) 1069, 1754, 2563, 2872, 2896
BstH2I RGCGCY 2 cut(s) 1029, 2538
BstHHI GCGC 6 cut(s) 499, 975, 1028, 1720, 2336, 2537
BstKTI GATC 9 cut(s) 751, 1012, 1069, 1536, 2050, 2125, 2161, 2446, 2659
BstMAI GTCTC 3 cut(s) 791, 1426, 1757
BstMBI GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2656
BstMWI GCNNNNNNNGC 9 cut(s) 77, 220, 562, 1034, 1474, 1694, 1708, 1717, 1726
BstNI CCWGG 1 cut(s) 2112
BstNSI RCATGY 2 cut(s) 1746, 2201
BstSCI CCNGG 3 cut(s) 145, 2110, 2436
BstSFI CTRYAG 1 cut(s) 2538
BstSLI GKGCMC 2 cut(s) 1923, 2205
BstSNI TACGTA 1 cut(s) 505
BstV2I GAAGAC 6 cut(s) 48, 513, 938, 2443, 2694, 2757
BstX2I RGATCY 3 cut(s) 1009, 1533, 2443
BstXI CCANNNNNNTGG 1 cut(s) 2396
BstYI RGATCY 3 cut(s) 1009, 1533, 2443
BsuRI GGCC 5 cut(s) 93, 778, 2384, 2420, 2778
BtgI CCRYGG 1 cut(s) 2220
BtgZI GCGATG 1 cut(s) 2934
BtrI CACGTC 1 cut(s) 2554
BtsCI GGATG 5 cut(s) 1069, 1754, 2563, 2872, 2896
BtsI GCAGTG 1 cut(s) 1092
BtsIMutI CAGTG 4 cut(s) 1092, 1564, 2713, 2743
BveI ACCTGC 3 cut(s) 1702, 2371, 2531
Cac8I GCNNGC 4 cut(s) 31, 408, 1683, 2108
CaiI CAGNNNCTG 1 cut(s) 578
CfoI GCGC 6 cut(s) 499, 975, 1028, 1720, 2336, 2537
Cfr13I GGNCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2662, 2722
CpoI CGGWCCG 1 cut(s) 60
CseI GACGC 3 cut(s) 1043, 1166, 2139
Csp6I GTAC 4 cut(s) 502, 1250, 1582, 2186
CspI CGGWCCG 1 cut(s) 60
CviQI GTAC 4 cut(s) 502, 1250, 1582, 2186
DpnI GATC 9 cut(s) 750, 1011, 1068, 1535, 2049, 2124, 2160, 2445, 2658
DpnII GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2656
DriI GACNNNNNGTC 3 cut(s) 517, 1972, 2037
EaeI YGGCCR 3 cut(s) 776, 2382, 2418
Eam1104I CTCTTC 6 cut(s) 464, 527, 822, 1760, 2583, 3041
Eam1105I GACNNNNNGTC 3 cut(s) 517, 1972, 2037
EarI CTCTTC 6 cut(s) 464, 527, 822, 1760, 2583, 3041
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 505
Eco130I CCWWGG 1 cut(s) 2220
Eco24I GRGCYC 2 cut(s) 2102, 2142
Eco32I GATATC 2 cut(s) 544, 594
Eco47I GGWCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2662, 2722
Eco57I CTGAAG 6 cut(s) 1706, 1793, 1941, 2013, 2193, 2544
Eco88I CYCGRG 2 cut(s) 2239, 2402
EcoO109I RGGNCCY 1 cut(s) 642
EcoRI GAATTC 1 cut(s) 2479
EcoRII CCWGG 1 cut(s) 2110
EcoRV GATATC 2 cut(s) 544, 594
EcoT14I CCWWGG 1 cut(s) 2220
EcoT22I ATGCAT 2 cut(s) 697, 1744
EcoT38I GRGCYC 2 cut(s) 2102, 2142
ErhI CCWWGG 1 cut(s) 2220
FalI AAGNNNNNCTT 2 cut(s) 752, 784
FaqI GGGAC 3 cut(s) 135, 589, 628
FbaI TGATCA 1 cut(s) 1066
FblI GTMKAC 1 cut(s) 2256
FokI GGATG 5 cut(s) 1056, 1761, 2570, 2879, 2903
FriOI GRGCYC 2 cut(s) 2102, 2142
FspBI CTAG 1 cut(s) 992
GlaI GCGC 6 cut(s) 498, 974, 1027, 1719, 2335, 2536
GsuI CTGGAG 3 cut(s) 1408, 2133, 2964
HaeII RGCGCY 2 cut(s) 1029, 2538
HaeIII GGCC 5 cut(s) 93, 778, 2384, 2420, 2778
HapII CCGG 4 cut(s) 58, 117, 147, 2438
HgaI GACGC 3 cut(s) 1043, 1166, 2139
HhaI GCGC 6 cut(s) 499, 975, 1028, 1720, 2336, 2537
Hin6I GCGC 6 cut(s) 497, 973, 1026, 1718, 2334, 2535
HinP1I GCGC 6 cut(s) 497, 973, 1026, 1718, 2334, 2535
HincII GTYRAC 2 cut(s) 997, 1159
HindII GTYRAC 2 cut(s) 997, 1159
HindIII AAGCTT 2 cut(s) 755, 2372
HpaI GTTAAC 2 cut(s) 997, 1159
HpaII CCGG 4 cut(s) 58, 117, 147, 2438
HphI GGTGA 8 cut(s) 442, 679, 1102, 1286, 2582, 2651, 2834, 2849
Hpy166II GTNNAC 8 cut(s) 997, 1105, 1159, 1672, 1921, 2203, 2257, 2351
Hpy8I GTNNAC 8 cut(s) 997, 1105, 1159, 1672, 1921, 2203, 2257, 2351
Hpy99I CGWCG 3 cut(s) 68, 2133, 2822
HpyCH4III ACNGT 5 cut(s) 984, 2185, 2639, 2738, 2822
HpyCH4IV ACGT 5 cut(s) 66, 504, 1126, 1500, 2553
HpyF10VI GCNNNNNNNGC 9 cut(s) 77, 220, 562, 1034, 1474, 1694, 1708, 1717, 1726
HpySE526I ACGT 5 cut(s) 66, 504, 1126, 1500, 2553
HspAI GCGC 6 cut(s) 497, 973, 1026, 1718, 2334, 2535
Ksp22I TGATCA 1 cut(s) 1066
KspAI GTTAAC 2 cut(s) 997, 1159
Kzo9I GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2656
LmnI GCTCC 5 cut(s) 187, 964, 970, 2176, 2945
MaeI CTAG 1 cut(s) 992
MaeII ACGT 5 cut(s) 66, 504, 1126, 1500, 2553
MaeIII GTNAC 8 cut(s) 667, 741, 903, 1264, 2029, 2639, 2822, 3036
MalI GATC 9 cut(s) 750, 1011, 1068, 1535, 2049, 2124, 2160, 2445, 2658
MboI GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2656
MflI RGATCY 3 cut(s) 1009, 1533, 2443
MhlI GDGCHC 5 cut(s) 54, 1923, 2102, 2142, 2205
MlsI TGGCCA 1 cut(s) 2384
MluCI AATT 8 cut(s) 226, 722, 1361, 1492, 2355, 2390, 2394, 2479
MluNI TGGCCA 1 cut(s) 2384
MlyI GAGTC 7 cut(s) 527, 851, 1100, 1126, 1407, 1826, 1982
MmeI TCCRAC 1 cut(s) 87
Mox20I TGGCCA 1 cut(s) 2384
Mph1103I ATGCAT 2 cut(s) 697, 1744
MroXI GAANNNNTTC 2 cut(s) 726, 1851
MscI TGGCCA 1 cut(s) 2384
MslI CAYNNNNRTG 2 cut(s) 1747, 1926
Msp20I TGGCCA 1 cut(s) 2384
MspA1I CMGCKG 4 cut(s) 775, 1477, 1688, 1979
MspCI CTTAAG 1 cut(s) 758
MspI CCGG 4 cut(s) 58, 117, 147, 2438
MspR9I CCNGG 3 cut(s) 147, 2112, 2438
Mva1269I GAATGC 1 cut(s) 442
MvaI CCWGG 1 cut(s) 2112
MwoI GCNNNNNNNGC 9 cut(s) 77, 220, 562, 1034, 1474, 1694, 1708, 1717, 1726
NciI CCSGG 2 cut(s) 147, 2438
NcoI CCATGG 1 cut(s) 2220
NdeII GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2656
NlaIV GGNNCC 3 cut(s) 151, 644, 2401
NmeAIII GCCGAG 2 cut(s) 69, 2446
NmuCI GTSAC 5 cut(s) 667, 2029, 2639, 2822, 3036
NsiI ATGCAT 2 cut(s) 697, 1744
NspI RCATGY 2 cut(s) 1746, 2201
NspV TTCGAA 1 cut(s) 524
PciI ACATGT 1 cut(s) 2197
PctI GAATGC 1 cut(s) 442
PdmI GAANNNNTTC 2 cut(s) 726, 1851
PfeI GAWTC 8 cut(s) 8, 1349, 1367, 1546, 1940, 1952, 2407, 2609
PleI GAGTC 7 cut(s) 526, 850, 1100, 1126, 1406, 1825, 1981
PpsI GAGTC 7 cut(s) 526, 850, 1100, 1126, 1406, 1825, 1981
Ppu21I YACGTR 1 cut(s) 505
PpuMI RGGWCCY 1 cut(s) 642
PscI ACATGT 1 cut(s) 2197
PshBI ATTAAT 3 cut(s) 1257, 1517, 2393
Psp5II RGGWCCY 1 cut(s) 642
Psp6I CCWGG 1 cut(s) 2110
PspGI CCWGG 1 cut(s) 2110
PspN4I GGNNCC 3 cut(s) 151, 644, 2401
PspPI GGNCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2662, 2722
PspPPI RGGWCCY 1 cut(s) 642
PsrI GAACNNNNNNTAC 2 cut(s) 2240, 2272
PstI CTGCAG 1 cut(s) 2542
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 3 cut(s) 1009, 1533, 2443
PvuII CAGCTG 2 cut(s) 1477, 1688
RsaI GTAC 4 cut(s) 503, 1251, 1583, 2187
RsaNI GTAC 4 cut(s) 502, 1250, 1582, 2186
RseI CAYNNNNRTG 2 cut(s) 1747, 1926
Rsr2I CGGWCCG 1 cut(s) 60
RsrII CGGWCCG 1 cut(s) 60
Sau3AI GATC 9 cut(s) 748, 1009, 1066, 1533, 2047, 2122, 2158, 2443, 2656
Sau96I GGNCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2662, 2722
ScaI AGTACT 1 cut(s) 2187
SchI GAGTC 7 cut(s) 527, 851, 1100, 1126, 1407, 1826, 1982
ScrFI CCNGG 3 cut(s) 147, 2112, 2438
SduI GDGCHC 5 cut(s) 54, 1923, 2102, 2142, 2205
SfcI CTRYAG 1 cut(s) 2538
SfuI TTCGAA 1 cut(s) 524
SinI GGWCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2662, 2722
SmiMI CAYNNNNRTG 2 cut(s) 1747, 1926
SmlI CTYRAG 3 cut(s) 758, 881, 1835
SmoI CTYRAG 3 cut(s) 758, 881, 1835
SnaBI TACGTA 1 cut(s) 505
SpeI ACTAGT 1 cut(s) 991
Sse9I AATT 8 cut(s) 226, 722, 1361, 1492, 2355, 2390, 2394, 2479
SsiI CCGC 9 cut(s) 5, 27, 440, 509, 775, 1977, 2346, 2568, 2768
SspMI CTAG 1 cut(s) 992
StyD4I CCNGG 3 cut(s) 145, 2110, 2436
StyI CCWWGG 1 cut(s) 2220
TaaI ACNGT 5 cut(s) 984, 2185, 2639, 2738, 2822
TaiI ACGT 5 cut(s) 69, 507, 1129, 1503, 2556
TaqI TCGA 8 cut(s) 132, 393, 524, 1224, 1527, 1855, 2628, 3060
TasI AATT 8 cut(s) 226, 722, 1361, 1492, 2355, 2390, 2394, 2479
TatI WGTACW 2 cut(s) 1581, 2185
TauI GCSGC 1 cut(s) 778
TfiI GAWTC 8 cut(s) 8, 1349, 1367, 1546, 1940, 1952, 2407, 2609
TscAI CASTG 4 cut(s) 1092, 1564, 2713, 2743
TseFI GTSAC 5 cut(s) 667, 2029, 2639, 2822, 3036
Tsp45I GTSAC 5 cut(s) 667, 2029, 2639, 2822, 3036
TspGWI ACGGA 1 cut(s) 2941
TspRI CASTG 4 cut(s) 1092, 1564, 2713, 2743
Vha464I CTTAAG 1 cut(s) 758
VneI GTGCAC 2 cut(s) 1919, 2201
VpaK11BI GGWCC 8 cut(s) 60, 149, 642, 1199, 1453, 2399, 2662, 2722
VspI ATTAAT 3 cut(s) 1257, 1517, 2393
XapI RAATTY 3 cut(s) 226, 1492, 2479
XceI RCATGY 2 cut(s) 1746, 2201
XmiI GTMKAC 1 cut(s) 2256
XmnI GAANNNNTTC 2 cut(s) 726, 1851
XspI CTAG 1 cut(s) 992
ZrmI AGTACT 1 cut(s) 2187
Zsp2I ATGCAT 2 cut(s) 697, 1744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.