pycom17g24380

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008048
Physical Location & Seq
Reverse (-)
22702420 .. 22703345
926 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 522 bp
ATGCACCCAAACTTCAGTGCAGAATCCATGGCAAAGATCACTAAGAGACTCTGCACCCTGGTTCTACGGCTGATGGCCGTCGGTGCTACCCTTTTGGCTGCAATTGTCATGGTTACAAGCCATCAGAGAACCAGCATCTTTGGAATATCTTTTGAAGTCAAGTATAACCATACTCCAGCCTTCCAGTACTTTGTGATCGCAAATGCAATTGCAACTGTATACGGGTTTCTGGTTATTTTTCTTCCTTCTAAGAGTCAGCTATGGAGATTAGTCGTGGCTTTGGATGTGGTAATCACTATACTACTGAGCTCAAGCATTTCAGCAGCTTTCGCTATTGGTACTCTGGGGAAGAATGGAAATTCATATGCGGGTTGGCTACCCATATGTGGGCAAGTCCCAAAATACTGTGACCAAGTAAAGGGGGCCCTAATCTCAAGCTTTGTTGGCCTCATCATATATGTCCTGCTGCTTCTGTATGCCATCCACACTGTCCTCGATCCTCTCCTCTTGCAAAAAACTTAA

Protein Analysis

174

Amino Acids

18.85

Weight (kDa)

9.51

Isoelectric Point (pI)

29.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 16 - 151 3.8e-40 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 219
AciI CCGC 1 cut(s) 368
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 75
AcsI RAATTY 1 cut(s) 358
AfaI GTAC 2 cut(s) 188, 340
AfiI CCNNNNNNNGG 3 cut(s) 386, 387, 418
AgsI TTSAA 1 cut(s) 155
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 4 cut(s) 259, 309, 326, 438
AluI AGCT 4 cut(s) 259, 309, 326, 438
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 1 cut(s) 491
AoxI GGCC 3 cut(s) 75, 423, 445
ApaI GGGCCC 1 cut(s) 427
ApeKI GCWGC 3 cut(s) 98, 323, 466
ApoI RAATTY 1 cut(s) 358
AspS9I GGNCC 2 cut(s) 423, 424
BaeGI GKGCMC 1 cut(s) 427
BanII GRGCYC 2 cut(s) 311, 427
Bbv12I GWGCWC 1 cut(s) 311
BbvI GCAGC 3 cut(s) 85, 335, 453
BccI CCATC 3 cut(s) 67, 129, 488
BceAI ACGGC 2 cut(s) 62, 83
BciT130I CCWGG 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 40
BisI GCNGC 3 cut(s) 99, 324, 467
BlsI GCNGC 3 cut(s) 100, 325, 468
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 423, 424
BmiI GGNNCC 2 cut(s) 424, 425
BmrFI CCNGG 1 cut(s) 59
BmsI GCATC 1 cut(s) 144
BpmI CTGGAG 1 cut(s) 159
BpuEI CTTGAG 2 cut(s) 295, 418
BsaJI CCNNGG 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 387, 418
Bse1I ACTGG 1 cut(s) 184
BseBI CCWGG 1 cut(s) 59
BseDI CCNNGG 2 cut(s) 27, 57
BseGI GGATG 2 cut(s) 289, 480
BseLI CCNNNNNNNGG 3 cut(s) 386, 387, 418
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 1 cut(s) 184
BseRI GAGGAG 1 cut(s) 494
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 3 cut(s) 85, 335, 453
BsgI GTGCAG 2 cut(s) 37, 39
BshFI GGCC 3 cut(s) 77, 425, 447
BsiHKAI GWGCWC 1 cut(s) 311
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 3 cut(s) 386, 387, 418
BsmAI GTCTC 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 3 cut(s) 77, 425, 447
Bsp120I GGGCCC 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 311, 427
Bsp143I GATC 3 cut(s) 36, 195, 496
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 1 cut(s) 368
BspANI GGCC 3 cut(s) 77, 425, 447
BspCNI CTCAG 1 cut(s) 297
BspLI GGNNCC 2 cut(s) 424, 425
BspPI GGATC 1 cut(s) 491
BsrI ACTGG 1 cut(s) 184
BssECI CCNNGG 2 cut(s) 27, 57
BssMI GATC 3 cut(s) 36, 195, 496
BssNAI GTATAC 1 cut(s) 220
BssT1I CCWWGG 1 cut(s) 27
Bst1107I GTATAC 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 3 cut(s) 217, 407, 490
BstDEI CTNAG 3 cut(s) 42, 249, 305
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 2 cut(s) 289, 480
BstKTI GATC 3 cut(s) 39, 198, 499
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 3 cut(s) 36, 195, 496
BstMWI GCNNNNNNNGC 3 cut(s) 83, 329, 444
BstNI CCWGG 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 57
BstSLI GKGCMC 1 cut(s) 427
BstV1I GCAGC 3 cut(s) 85, 335, 453
BstZ17I GTATAC 1 cut(s) 220
BsuRI GGCC 3 cut(s) 77, 425, 447
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 2 cut(s) 289, 480
BtsIMutI CAGTG 2 cut(s) 22, 486
Cfr13I GGNCC 2 cut(s) 423, 424
Csp6I GTAC 2 cut(s) 187, 339
CviAII CATG 2 cut(s) 28, 109
CviQI GTAC 2 cut(s) 187, 339
DdeI CTNAG 3 cut(s) 42, 249, 305
DpnI GATC 3 cut(s) 38, 197, 498
DpnII GATC 3 cut(s) 36, 195, 496
EaeI YGGCCR 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 2 cut(s) 311, 427
Eco53kI GAGCTC 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 309
EcoO109I RGGNCCY 2 cut(s) 423, 424
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 2 cut(s) 311, 427
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 2 cut(s) 31, 112
FaqI GGGAC 1 cut(s) 380
FatI CATG 2 cut(s) 27, 108
FauI CCCGC 1 cut(s) 361
FauNDI CATATG 2 cut(s) 364, 383
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 99, 324, 467
FokI GGATG 2 cut(s) 296, 467
FriOI GRGCYC 2 cut(s) 311, 427
Fsp4HI GCNGC 3 cut(s) 99, 324, 467
GluI GCNGC 3 cut(s) 99, 324, 467
GsuI CTGGAG 1 cut(s) 159
HaeIII GGCC 3 cut(s) 77, 425, 447
Hin1II CATG 2 cut(s) 31, 112
HindIII AAGCTT 1 cut(s) 436
HinfI GANTC 3 cut(s) 23, 48, 253
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 1 cut(s) 126
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 190, 255
HpyCH4III ACNGT 3 cut(s) 217, 407, 490
HpyCH4V TGCA 7 cut(s) 4, 20, 54, 101, 206, 212, 511
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 329, 444
HpyF3I CTNAG 3 cut(s) 42, 249, 305
Hsp92II CATG 2 cut(s) 31, 112
Kzo9I GATC 3 cut(s) 36, 195, 496
LpnPI CCDG 8 cut(s) 44, 71, 145, 189, 197, 215, 329, 476
Lsp1109I GCAGC 3 cut(s) 85, 335, 453
LweI GCATC 1 cut(s) 144
MaeIII GTNAC 2 cut(s) 112, 407
MalI GATC 3 cut(s) 38, 197, 498
MboI GATC 3 cut(s) 36, 195, 496
MboII GAAGA 2 cut(s) 233, 361
MfeI CAATTG 2 cut(s) 102, 207
MhlI GDGCHC 2 cut(s) 311, 427
MluCI AATT 3 cut(s) 102, 207, 358
MlyI GAGTC 2 cut(s) 42, 262
MnlI CCTC 4 cut(s) 458, 503, 510, 515
MseI TTAA 1 cut(s) 520
MspR9I CCNGG 1 cut(s) 59
MunI CAATTG 2 cut(s) 102, 207
MvaI CCWGG 1 cut(s) 59
MwoI GCNNNNNNNGC 3 cut(s) 83, 329, 444
NcoI CCATGG 1 cut(s) 27
NdeI CATATG 2 cut(s) 364, 383
NdeII GATC 3 cut(s) 36, 195, 496
NlaIII CATG 2 cut(s) 31, 112
NlaIV GGNNCC 2 cut(s) 424, 425
NmuCI GTSAC 1 cut(s) 407
PfeI GAWTC 1 cut(s) 23
PkrI GCNGC 3 cut(s) 100, 325, 468
PleI GAGTC 2 cut(s) 42, 261
PpsI GAGTC 2 cut(s) 42, 261
Psp124BI GAGCTC 1 cut(s) 311
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspN4I GGNNCC 2 cut(s) 424, 425
PspOMI GGGCCC 1 cut(s) 423
PspPI GGNCC 2 cut(s) 423, 424
RsaI GTAC 2 cut(s) 188, 340
RsaNI GTAC 2 cut(s) 187, 339
SacI GAGCTC 1 cut(s) 311
SaqAI TTAA 1 cut(s) 520
SatI GCNGC 3 cut(s) 99, 324, 467
Sau3AI GATC 3 cut(s) 36, 195, 496
Sau96I GGNCC 2 cut(s) 423, 424
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 2 cut(s) 42, 262
ScrFI CCNGG 1 cut(s) 59
SduI GDGCHC 2 cut(s) 311, 427
SetI ASST 4 cut(s) 261, 311, 328, 440
SfaNI GCATC 1 cut(s) 144
SmlI CTYRAG 2 cut(s) 310, 433
SmoI CTYRAG 2 cut(s) 310, 433
Sse9I AATT 3 cut(s) 102, 207, 358
SsiI CCGC 1 cut(s) 368
SstI GAGCTC 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 3 cut(s) 217, 407, 490
TaqI TCGA 1 cut(s) 495
TasI AATT 3 cut(s) 102, 207, 358
TatI WGTACW 1 cut(s) 186
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 1 cut(s) 520
Tru9I TTAA 1 cut(s) 520
TscAI CASTG 2 cut(s) 22, 493
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 3 cut(s) 98, 323, 466
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 1 cut(s) 351
TspRI CASTG 2 cut(s) 22, 493
XapI RAATTY 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 219
ZrmI AGTACT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.