pycom17g24590

Clathrin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0009774
Physical Location & Seq
Reverse (-)
22843815 .. 22846076
2262 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1272 bp
ATGTCGTCCTTCGACTCCTTCCCCAACGACAACGAATCCCACGACCCCCACAGCCACTCCCCCGCCGGCCAGCCCTTCGACGACGATGCTTACATGGCACACGACCCCGGCCTTCCTTCCCATAACTACGACTCGTCGTTCAACCCTCCCACCCACCACGACGACTTCTCCCCCGCTGCCATCGACCCTCCCCCTCTCCTCCACTCTGATTTCCAGGACGACGTCGTTCTCCCCGGTGGCGACGACCACCACAACATTGATCAGCAGCAGCAGTCCCCCGAGGTGTACGGCTTCGGCATTTCCACCCCTAACCCGGAATTCGTGTCACCTTTTGATTCGGCGGTGCCCGAGGAAAATGGCGTCGTGGGTGGAGATGGCGGCATTGGTGATGATGACGGGGGCATTTTCGCCTCCAGTGAGCCGGTACTGCCTCCGCCCGAGGAGATGCGGGAGGAAGGCTTTGCTCGCCGTGAGTGGCGTCGCCTAAATGTCATTCATCTAGAGGAAAAGGAAAAAAGGGAAAGGGAGATGCGGAACCAGATTATCGAGGAAGCTGAAGAATTTAAGCGGGCCTTCTATGAGAAACGGAAGCTCAATTGTGAAACTAACAAAGCTCACAACAGAGAAAGGGAGAAGCTGTACCTGGCCAACCAAGAGAAGTTCCACAAAGAAGCTGACAAACATTACTGGAAAGCAATAGCGGAGCTGATTCCTCGTGAGGTTCCTAATATTGAGAAGAAGAGAGGGAAGAAGGATCCAGACAAGAAGCCCTCAATTGTTGTCATCCAGGGCCCAAAACCTGGTAAACCGACTGATCTTTCAAGGATGCGGCAGATTTTCCTGCAGCTGAAACAGAACCCACCGCCACACATGATGCCACTCCCACCTTCCAAAGATGGTAAGGATGCAAGGGGAGAAAAAGATGGAAAGGAGTCAAAGGATGGCAATGATACAAAAGATGCAAAGGGAGGAAAGGGAGCAAAGGATACCAAGGAAGGAGGAATGGACGCAAAGGATGCCAAGGAAGGAGGAAATGACGCGAAGGATACCAAGGAAGGAGGAAAGGATGCAAAGGAAGCAAAGGAAGCAAAGGATGCCAAGAATGGAAAAATTAATTCACCAACCGCAGCTGGAGCTGCAGCTGCAAGCGCACCTGTTTCACCTGCCAAAGATGTTACTGCCAATGGTACTCCCGGCTCATCAAAACCAGGGGCTTCAGCTGAACCAGAGGGTGAGCAACATTTGGAAACCACGCCAACTCCTGCGGAGTAA

Protein Analysis

424

Amino Acids

46.29

Weight (kDa)

5.24

Isoelectric Point (pI)

55.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clathrin_lg_ch PF01086 148 - 286 9e-06 Clathrin light chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1171
AatII GACGTC 1 cut(s) 225
Acc36I ACCTGC 1 cut(s) 1171
AccB1I GGYRCC 1 cut(s) 343
AccB7I CCANNNNNTGG 1 cut(s) 800
AccII CGCG 1 cut(s) 1040
AclWI GGATC 2 cut(s) 749, 762
AcoI YGGCCR 2 cut(s) 67, 645
AcsI RAATTY 2 cut(s) 317, 560
AcuI CTGAAG 2 cut(s) 576, 1200
AcyI GRCGYC 3 cut(s) 222, 360, 478
AfaI GTAC 4 cut(s) 287, 426, 641, 1189
AfiI CCNNNNNNNGG 2 cut(s) 313, 800
AgsI TTSAA 2 cut(s) 142, 822
AjnI CCWGG 5 cut(s) 213, 642, 786, 799, 1207
AlwI GGATC 2 cut(s) 749, 762
Ama87I CYCGRG 3 cut(s) 278, 347, 437
AoxI GGCC 5 cut(s) 67, 109, 570, 645, 790
ApaI GGGCCC 1 cut(s) 794
ApeKI GCWGC 8 cut(s) 176, 265, 268, 844, 1127, 1136, 1139, 1142
ApoI RAATTY 2 cut(s) 317, 560
AseI ATTAAT 1 cut(s) 1113
AspLEI GCGC 1 cut(s) 1151
AspS9I GGNCC 3 cut(s) 570, 790, 791
AsuC2I CCSGG 4 cut(s) 108, 234, 314, 1194
AsuHPI GGTGA 5 cut(s) 318, 398, 1110, 1152, 1244
AvaI CYCGRG 3 cut(s) 278, 347, 437
BaeGI GKGCMC 2 cut(s) 348, 794
BalI TGGCCA 1 cut(s) 647
BamHI GGATCC 1 cut(s) 754
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 794
BauI CACGAG 1 cut(s) 714
BbvI GCAGC 8 cut(s) 163, 277, 280, 856, 1123, 1129, 1139, 1151
BccI CCATC 5 cut(s) 188, 368, 890, 917, 935
BceAI ACGGC 2 cut(s) 304, 453
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 5 cut(s) 215, 644, 788, 801, 1209
BciVI GTATCC 2 cut(s) 979, 1039
BclI TGATCA 1 cut(s) 259
BcnI CCSGG 4 cut(s) 108, 234, 314, 1194
BfaI CTAG 1 cut(s) 500
BfmI CTRYAG 2 cut(s) 842, 1137
BfuAI ACCTGC 1 cut(s) 1171
BfuI GTATCC 2 cut(s) 979, 1039
Bme1390I CCNGG 9 cut(s) 108, 215, 234, 314, 644, 788, 801, 1194, 1209
BmeT110I CYCGRG 3 cut(s) 278, 347, 437
BmgT120I GGNCC 3 cut(s) 570, 790, 791
BmiI GGNNCC 5 cut(s) 345, 536, 723, 756, 792
BmrFI CCNGG 9 cut(s) 108, 215, 234, 314, 644, 788, 801, 1194, 1209
BpmI CTGGAG 2 cut(s) 397, 1152
BpuMI CCSGG 4 cut(s) 108, 234, 314, 1194
BsaHI GRCGYC 3 cut(s) 222, 360, 478
BsaXI ACNNNNNCTCC 7 cut(s) 41, 71, 152, 182, 623, 653, 1243
Bsc4I CCNNNNNNNGG 2 cut(s) 313, 800
Bse118I RCCGGY 2 cut(s) 65, 421
Bse1I ACTGG 2 cut(s) 414, 692
Bse3DI GCAATG 1 cut(s) 952
BseBI CCWGG 5 cut(s) 215, 644, 788, 801, 1209
BseGI GGATG 7 cut(s) 783, 831, 910, 946, 1021, 1072, 1099
BseLI CCNNNNNNNGG 2 cut(s) 313, 800
BseMI GCAATG 1 cut(s) 952
BseNI ACTGG 2 cut(s) 414, 692
BseRI GAGGAG 2 cut(s) 188, 455
BseSI GKGCMC 2 cut(s) 348, 794
BseXI GCAGC 8 cut(s) 163, 277, 280, 856, 1123, 1129, 1139, 1151
Bsh1236I CGCG 1 cut(s) 1040
BshFI GGCC 5 cut(s) 69, 111, 572, 647, 792
BshNI GGYRCC 1 cut(s) 343
BsiHKCI CYCGRG 3 cut(s) 278, 347, 437
BsiSI CCGG 6 cut(s) 66, 108, 234, 314, 422, 1194
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 2 cut(s) 313, 800
BsmFI GGGAC 1 cut(s) 259
BsnI GGCC 5 cut(s) 69, 111, 572, 647, 792
BsoBI CYCGRG 3 cut(s) 278, 347, 437
Bsp120I GGGCCC 1 cut(s) 790
Bsp1286I GDGCHC 2 cut(s) 348, 794
Bsp143I GATC 3 cut(s) 259, 754, 814
BspANI GGCC 5 cut(s) 69, 111, 572, 647, 792
BspFNI CGCG 1 cut(s) 1040
BspLI GGNNCC 5 cut(s) 345, 536, 723, 756, 792
BspMAI CTGCAG 2 cut(s) 846, 1141
BspMI ACCTGC 1 cut(s) 1171
BspPI GGATC 2 cut(s) 749, 762
BspT107I GGYRCC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 952
BsrFI RCCGGY 2 cut(s) 65, 421
BsrI ACTGG 2 cut(s) 414, 692
BssAI RCCGGY 2 cut(s) 65, 421
BssMI GATC 3 cut(s) 259, 754, 814
BssNI GRCGYC 3 cut(s) 222, 360, 478
BssSI CACGAG 1 cut(s) 714
BssT1I CCWWGG 3 cut(s) 990, 1020, 1050
Bst2BI CACGAG 1 cut(s) 714
Bst2UI CCWGG 5 cut(s) 215, 644, 788, 801, 1209
Bst6I CTCTTC 1 cut(s) 734
BstACI GRCGYC 3 cut(s) 222, 360, 478
BstAPI GCANNNNNTGC 2 cut(s) 1016, 1094
BstC8I GCNNGC 5 cut(s) 67, 71, 466, 570, 1147
BstF5I GGATG 7 cut(s) 783, 831, 910, 946, 1021, 1072, 1099
BstFNI CGCG 1 cut(s) 1040
BstHHI GCGC 1 cut(s) 1151
BstKTI GATC 3 cut(s) 262, 757, 817
BstMBI GATC 3 cut(s) 259, 754, 814
BstNI CCWGG 5 cut(s) 215, 644, 788, 801, 1209
BstSCI CCNGG 9 cut(s) 106, 213, 232, 312, 642, 786, 799, 1192, 1207
BstSFI CTRYAG 2 cut(s) 842, 1137
BstSLI GKGCMC 2 cut(s) 348, 794
BstUI CGCG 1 cut(s) 1040
BstV1I GCAGC 8 cut(s) 163, 277, 280, 856, 1123, 1129, 1139, 1151
BstX2I RGATCY 1 cut(s) 754
BstYI RGATCY 1 cut(s) 754
BsuI GTATCC 2 cut(s) 979, 1039
BsuRI GGCC 5 cut(s) 69, 111, 572, 647, 792
BtsCI GGATG 7 cut(s) 783, 831, 910, 946, 1021, 1072, 1099
BtsIMutI CAGTG 1 cut(s) 421
BveI ACCTGC 1 cut(s) 1171
Cac8I GCNNGC 5 cut(s) 67, 71, 466, 570, 1147
CfoI GCGC 1 cut(s) 1151
Cfr10I RCCGGY 2 cut(s) 65, 421
Cfr13I GGNCC 3 cut(s) 570, 790, 791
CseI GACGC 4 cut(s) 349, 467, 1016, 1046
CsiI ACCWGGT 1 cut(s) 799
Csp6I GTAC 4 cut(s) 286, 425, 640, 1188
CviAII CATG 2 cut(s) 94, 871
CviQI GTAC 4 cut(s) 286, 425, 640, 1188
DpnI GATC 3 cut(s) 261, 756, 816
DpnII GATC 3 cut(s) 259, 754, 814
EaeI YGGCCR 2 cut(s) 67, 645
Eam1104I CTCTTC 1 cut(s) 734
EarI CTCTTC 1 cut(s) 734
EciI GGCGGA 1 cut(s) 423
Eco130I CCWWGG 3 cut(s) 990, 1020, 1050
Eco24I GRGCYC 1 cut(s) 794
Eco57I CTGAAG 2 cut(s) 576, 1200
Eco88I CYCGRG 3 cut(s) 278, 347, 437
EcoO109I RGGNCCY 1 cut(s) 790
EcoRI GAATTC 1 cut(s) 317
EcoRII CCWGG 5 cut(s) 213, 642, 786, 799, 1207
EcoT14I CCWWGG 3 cut(s) 990, 1020, 1050
EcoT38I GRGCYC 1 cut(s) 794
ErhI CCWWGG 3 cut(s) 990, 1020, 1050
FaeI CATG 2 cut(s) 97, 874
FaiI YATR 4 cut(s) 95, 123, 579, 872
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FaqI GGGAC 1 cut(s) 259
FatI CATG 2 cut(s) 93, 870
FauI CCCGC 4 cut(s) 70, 181, 441, 561
FbaI TGATCA 1 cut(s) 259
FokI GGATG 7 cut(s) 770, 838, 917, 953, 1028, 1079, 1106
FriOI GRGCYC 1 cut(s) 794
FspBI CTAG 1 cut(s) 500
GlaI GCGC 1 cut(s) 1150
GsuI CTGGAG 2 cut(s) 397, 1152
HaeIII GGCC 5 cut(s) 69, 111, 572, 647, 792
HapII CCGG 6 cut(s) 66, 108, 234, 314, 422, 1194
HgaI GACGC 4 cut(s) 349, 467, 1016, 1046
HhaI GCGC 1 cut(s) 1151
Hin1I GRCGYC 3 cut(s) 222, 360, 478
Hin1II CATG 2 cut(s) 97, 874
Hin6I GCGC 1 cut(s) 1149
HinP1I GCGC 1 cut(s) 1149
HinfI GANTC 6 cut(s) 14, 35, 131, 335, 709, 932
HpaII CCGG 6 cut(s) 66, 108, 234, 314, 422, 1194
HphI GGTGA 5 cut(s) 318, 398, 1110, 1152, 1244
Hpy166II GTNNAC 2 cut(s) 286, 806
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 3 cut(s) 500, 716, 758
Hpy8I GTNNAC 2 cut(s) 286, 806
Hpy99I CGWCG 9 cut(s) 83, 86, 139, 164, 224, 227, 245, 365, 483
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 6 cut(s) 844, 908, 962, 1070, 1139, 1145
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 3 cut(s) 222, 360, 478
Hsp92II CATG 2 cut(s) 97, 874
HspAI GCGC 1 cut(s) 1149
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 3 cut(s) 259, 754, 814
LmnI GCTCC 3 cut(s) 703, 977, 1133
Lsp1109I GCAGC 8 cut(s) 163, 277, 280, 856, 1123, 1129, 1139, 1151
MabI ACCWGGT 1 cut(s) 799
MaeI CTAG 1 cut(s) 500
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 2 cut(s) 324, 1174
MalI GATC 3 cut(s) 261, 756, 816
MboI GATC 3 cut(s) 259, 754, 814
MboII GAAGA 4 cut(s) 569, 748, 751, 760
MfeI CAATTG 2 cut(s) 595, 774
MflI RGATCY 1 cut(s) 754
MhlI GDGCHC 2 cut(s) 348, 794
MlsI TGGCCA 1 cut(s) 647
MluCI AATT 6 cut(s) 317, 560, 595, 774, 1110, 1114
MluNI TGGCCA 1 cut(s) 647
MlyI GAGTC 3 cut(s) 8, 125, 941
Mox20I TGGCCA 1 cut(s) 647
MroNI GCCGGC 1 cut(s) 65
MscI TGGCCA 1 cut(s) 647
MseI TTAA 2 cut(s) 564, 1113
Msp20I TGGCCA 1 cut(s) 647
MspA1I CMGCKG 5 cut(s) 176, 847, 1130, 1142, 1220
MspI CCGG 6 cut(s) 66, 108, 234, 314, 422, 1194
MspR9I CCNGG 9 cut(s) 108, 215, 234, 314, 644, 788, 801, 1194, 1209
MunI CAATTG 2 cut(s) 595, 774
MvaI CCWGG 5 cut(s) 215, 644, 788, 801, 1209
MvnI CGCG 1 cut(s) 1040
NaeI GCCGGC 1 cut(s) 67
NciI CCSGG 4 cut(s) 108, 234, 314, 1194
NdeII GATC 3 cut(s) 259, 754, 814
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 2 cut(s) 97, 874
NlaIV GGNNCC 5 cut(s) 345, 536, 723, 756, 792
NmuCI GTSAC 1 cut(s) 324
PaqCI CACCTGC 1 cut(s) 1171
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 3 cut(s) 35, 335, 709
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 800
PfoI TCCNGGA 1 cut(s) 213
PleI GAGTC 3 cut(s) 8, 125, 940
PpsI GAGTC 3 cut(s) 8, 125, 940
PshBI ATTAAT 1 cut(s) 1113
Psp6I CCWGG 5 cut(s) 213, 642, 786, 799, 1207
PspGI CCWGG 5 cut(s) 213, 642, 786, 799, 1207
PspN4I GGNNCC 5 cut(s) 345, 536, 723, 756, 792
PspOMI GGGCCC 1 cut(s) 790
PspPI GGNCC 3 cut(s) 570, 790, 791
PstI CTGCAG 2 cut(s) 846, 1141
PsuI RGATCY 1 cut(s) 754
PsyI GACNNNGTC 1 cut(s) 221
PvuII CAGCTG 4 cut(s) 847, 1130, 1142, 1220
RsaI GTAC 4 cut(s) 287, 426, 641, 1189
RsaNI GTAC 4 cut(s) 286, 425, 640, 1188
SaqAI TTAA 2 cut(s) 564, 1113
Sau3AI GATC 3 cut(s) 259, 754, 814
Sau96I GGNCC 3 cut(s) 570, 790, 791
SchI GAGTC 3 cut(s) 8, 125, 941
ScrFI CCNGG 9 cut(s) 108, 215, 234, 314, 644, 788, 801, 1194, 1209
SduI GDGCHC 2 cut(s) 348, 794
SexAI ACCWGGT 1 cut(s) 799
SfcI CTRYAG 2 cut(s) 842, 1137
Sse9I AATT 6 cut(s) 317, 560, 595, 774, 1110, 1114
SspI AATATT 1 cut(s) 730
SspMI CTAG 1 cut(s) 500
StyD4I CCNGG 9 cut(s) 106, 213, 232, 312, 642, 786, 799, 1192, 1207
StyI CCWWGG 3 cut(s) 990, 1020, 1050
TaiI ACGT 1 cut(s) 225
TaqI TCGA 4 cut(s) 12, 78, 183, 546
TasI AATT 6 cut(s) 317, 560, 595, 774, 1110, 1114
TauI GCSGC 2 cut(s) 381, 832
TfiI GAWTC 3 cut(s) 35, 335, 709
Tru1I TTAA 2 cut(s) 564, 1113
Tru9I TTAA 2 cut(s) 564, 1113
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 1 cut(s) 324
TseI GCWGC 8 cut(s) 176, 265, 268, 844, 1127, 1136, 1139, 1142
Tsp45I GTSAC 1 cut(s) 324
TspDTI ATGAA 1 cut(s) 485
TspGWI ACGGA 1 cut(s) 601
TspRI CASTG 1 cut(s) 421
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 800
VspI ATTAAT 1 cut(s) 1113
XapI RAATTY 2 cut(s) 317, 560
XbaI TCTAGA 1 cut(s) 499
XspI CTAG 1 cut(s) 500
ZraI GACGTC 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.