pycom17g26830

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008491
Physical Location & Seq
Reverse (-)
25145605 .. 25147799
2195 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1140 bp
ATGCTTTTTATTAATATCGAAATTTATGAGAGACATTCAAAGATGGAAGTGACAATGTGGATGGCTTTAGCTACCACGATTTTCACAACCATGGTAGCCGTGCCTTCATCGGCAGCGAATACCGTGACCTTCGTCTTCGGTGACTCCTTGACAGATGTAGGGAACAACAACTATCTGCAGTATTCTCTTGCAAAGTCAAATTATCCCTGGTATGGTGTCGATTATGTTGGTGGTCGACCTACTGGGAGGTTCAGTAATGGAAGAACCATCGGCGACTTAATATCCGAAAAGCTTGGGATTCCATCGCCACCACCTTATCTATCCTTGTCACGGAACAATGATGCACTGCTTAAAGGGGTCAATTATGCATCTGGTGGAGCAGGAATACTCAATAGCACTGGACTCTATTTTATTCAGAGGCTGAGTTTTGATGATCAAATACAAAATTTCAAAAAGACAACGGAAGTCATCAAGGCTAAGATTGGGGCACAGGCTGCAGCCAAGCTCTTCAAGGAAGCCATCTTCTTCTTGGGACTTGGGAGCAATGATTATATCAACAATTATTTGCAACCCTTTTTACCTACTGGTCGGCAGTACACTCCCGAACAATTTGTGGAATTATTGATGCGTACCTTAGAGGGACAACTTAAGACGCTCTACCACCTGGGTGCCCGAAATATGACGTTCCATGGTCTCGGACCACTTGGCTGCATTCCTTCTCAAAGAGTCAGATCAAACACAGGGCAGTGCTTAAACCAAGTAAACAATATGATGCTACAATTCAACTCCAATGTAGAAAAGCTCCTTGCCTCCCTTGACAGGCAACTCCCAGGGGCAAAAATCTCATTTGCAGACACATACGGAGACGTCCTCGACTTAATCCAAAACCCTACAGCTTACGGGTTTAAGGTTTCGAACCTTCCGTGTTGCAATGTGGCCACAAACATTGGAGGGCTATGCTTGCCTAATTCACAGCTATGCAGCAACCGGAGAGAGTACGTGTTCTGGGATGCATTCCACCCTACCGACGCAGCAAACGCGGCTCTCGCAGATAAACTCTTCAAGAAACTATTCACGCCTCCTTCCGGTGCATCAACACTATTCACGGCTCCTTCCGGTGCACCAGCACCTTCACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

380

Amino Acids

41.52

Weight (kDa)

8.76

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 353 5.4e-39 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 870
AccB1I GGYRCC 1 cut(s) 668
AccI GTMKAC 1 cut(s) 235
AccII CGCG 1 cut(s) 1040
AciI CCGC 1 cut(s) 1040
AcoI YGGCCR 1 cut(s) 936
AcsI RAATTY 2 cut(s) 21, 445
AcyI GRCGYC 1 cut(s) 867
AfaI GTAC 3 cut(s) 596, 631, 998
AfiI CCNNNNNNNGG 4 cut(s) 212, 330, 819, 1085
AflII CTTAAG 1 cut(s) 647
AflIII ACRYGT 1 cut(s) 999
AgsI TTSAA 5 cut(s) 39, 451, 511, 784, 1063
AjnI CCWGG 3 cut(s) 206, 663, 829
AleI CACNNNNGTG 1 cut(s) 666
AluBI AGCT 6 cut(s) 71, 292, 505, 802, 896, 976
AluI AGCT 6 cut(s) 71, 292, 505, 802, 896, 976
Alw21I GWGCWC 1 cut(s) 1123
Alw26I GTCTC 3 cut(s) 25, 698, 858
Alw44I GTGCAC 1 cut(s) 1119
AlwNI CAGNNNCTG 1 cut(s) 421
AoxI GGCC 1 cut(s) 936
ApaLI GTGCAC 1 cut(s) 1119
ApeKI GCWGC 6 cut(s) 113, 494, 497, 708, 981, 1031
ApoI RAATTY 2 cut(s) 21, 445
AseI ATTAAT 1 cut(s) 12
Asp700I GAANNNNTTC 1 cut(s) 1070
AspS9I GGNCC 1 cut(s) 698
AsuHPI GGTGA 2 cut(s) 152, 1125
AsuII TTCGAA 1 cut(s) 914
AvaII GGWCC 1 cut(s) 698
BaeGI GKGCMC 3 cut(s) 490, 673, 1123
BalI TGGCCA 1 cut(s) 938
BanI GGYRCC 1 cut(s) 668
BbsI GAAGAC 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 1123
BbvI GCAGC 6 cut(s) 125, 481, 509, 695, 993, 1043
BccI CCATC 5 cut(s) 37, 55, 275, 310, 527
BceAI ACGGC 2 cut(s) 83, 1122
BciT130I CCWGG 3 cut(s) 208, 665, 831
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 3 cut(s) 25, 698, 858
BfaI CTAG 1 cut(s) 1138
BfmI CTRYAG 3 cut(s) 176, 495, 891
BfrI CTTAAG 1 cut(s) 647
BisI GCNGC 7 cut(s) 114, 495, 498, 709, 982, 1032, 1041
BlsI GCNGC 7 cut(s) 115, 496, 499, 710, 983, 1033, 1042
Bme1390I CCNGG 3 cut(s) 208, 665, 831
Bme18I GGWCC 1 cut(s) 698
BmgT120I GGNCC 1 cut(s) 698
BmiI GGNNCC 2 cut(s) 670, 1110
BmrFI CCNGG 3 cut(s) 208, 665, 831
BmrI ACTGGG 1 cut(s) 252
BmsI GCATC 6 cut(s) 331, 377, 615, 762, 1000, 1100
BmuI ACTGGG 1 cut(s) 252
BoxI GACNNNNGTC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 127
BplI GAGNNNNNCTC 2 cut(s) 855, 887
Bpu14I TTCGAA 1 cut(s) 914
BsaAI YACGTR 1 cut(s) 1000
BsaHI GRCGYC 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 698
BsaJI CCNNGG 6 cut(s) 90, 206, 664, 688, 829, 830
BsaWI WCCGGW 3 cut(s) 987, 1085, 1115
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 4 cut(s) 212, 330, 819, 1085
Bse1I ACTGG 3 cut(s) 247, 403, 589
Bse3DI GCAATG 2 cut(s) 550, 937
BseBI CCWGG 3 cut(s) 208, 665, 831
BseDI CCNNGG 6 cut(s) 90, 206, 664, 688, 829, 830
BseGI GGATG 2 cut(s) 66, 1015
BseLI CCNNNNNNNGG 4 cut(s) 212, 330, 819, 1085
BseMI GCAATG 2 cut(s) 550, 937
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 3 cut(s) 247, 403, 589
BseSI GKGCMC 3 cut(s) 490, 673, 1123
BseXI GCAGC 6 cut(s) 125, 481, 509, 695, 993, 1043
Bsh1236I CGCG 1 cut(s) 1040
BshFI GGCC 1 cut(s) 938
BshNI GGYRCC 1 cut(s) 668
BsiHKAI GWGCWC 1 cut(s) 1123
BsiSI CCGG 3 cut(s) 988, 1086, 1116
BslFI GGGAC 2 cut(s) 546, 654
BslI CCNNNNNNNGG 4 cut(s) 212, 330, 819, 1085
BsmAI GTCTC 3 cut(s) 25, 698, 858
BsmBI CGTCTC 1 cut(s) 858
BsmFI GGGAC 2 cut(s) 546, 654
BsmI GAATGC 2 cut(s) 711, 1013
BsnI GGCC 1 cut(s) 938
Bso31I GGTCTC 1 cut(s) 698
Bsp119I TTCGAA 1 cut(s) 914
Bsp1286I GDGCHC 3 cut(s) 490, 673, 1123
Bsp143I GATC 2 cut(s) 433, 731
Bsp19I CCATGG 2 cut(s) 90, 688
BspACI CCGC 1 cut(s) 1040
BspANI GGCC 1 cut(s) 938
BspCNI CTCAG 1 cut(s) 414
BspFNI CGCG 1 cut(s) 1040
BspLI GGNNCC 2 cut(s) 670, 1110
BspMAI CTGCAG 2 cut(s) 180, 499
BspQI GCTCTTC 1 cut(s) 512
BspT104I TTCGAA 1 cut(s) 914
BspT107I GGYRCC 1 cut(s) 668
BspTI CTTAAG 1 cut(s) 647
BspTNI GGTCTC 1 cut(s) 698
BsrDI GCAATG 2 cut(s) 550, 937
BsrI ACTGG 3 cut(s) 247, 403, 589
BssECI CCNNGG 6 cut(s) 90, 206, 664, 688, 829, 830
BssMI GATC 2 cut(s) 433, 731
BssNI GRCGYC 1 cut(s) 867
BssT1I CCWWGG 2 cut(s) 90, 688
Bst2UI CCWGG 3 cut(s) 208, 665, 831
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 2 cut(s) 512, 1064
BstACI GRCGYC 1 cut(s) 867
BstAFI CTTAAG 1 cut(s) 647
BstAPI GCANNNNNTGC 1 cut(s) 494
BstBAI YACGTR 1 cut(s) 1000
BstBI TTCGAA 1 cut(s) 914
BstC8I GCNNGC 1 cut(s) 962
BstDEI CTNAG 3 cut(s) 422, 477, 634
BstDSI CCRYGG 2 cut(s) 90, 688
BstF5I GGATG 2 cut(s) 66, 1015
BstFNI CGCG 1 cut(s) 1040
BstKTI GATC 2 cut(s) 436, 734
BstMAI GTCTC 3 cut(s) 25, 698, 858
BstMBI GATC 2 cut(s) 433, 731
BstMWI GCNNNNNNNGC 5 cut(s) 494, 961, 1037, 1040, 1046
BstNI CCWGG 3 cut(s) 208, 665, 831
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 3 cut(s) 206, 663, 829
BstSFI CTRYAG 3 cut(s) 176, 495, 891
BstSLI GKGCMC 3 cut(s) 490, 673, 1123
BstUI CGCG 1 cut(s) 1040
BstV1I GCAGC 6 cut(s) 125, 481, 509, 695, 993, 1043
BstV2I GAAGAC 1 cut(s) 127
BsuRI GGCC 1 cut(s) 938
BtgI CCRYGG 2 cut(s) 90, 688
BtgZI GCGATG 1 cut(s) 288
BtsCI GGATG 2 cut(s) 66, 1015
BtsI GCAGTG 2 cut(s) 344, 752
BtsIMutI CAGTG 3 cut(s) 344, 396, 752
Cac8I GCNNGC 1 cut(s) 962
CaiI CAGNNNCTG 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 698
CseI GACGC 2 cut(s) 661, 1037
Csp6I GTAC 3 cut(s) 595, 630, 997
CspCI CAANNNNNGTGG 2 cut(s) 928, 963
CviAII CATG 2 cut(s) 91, 689
CviQI GTAC 3 cut(s) 595, 630, 997
DdeI CTNAG 3 cut(s) 422, 477, 634
DpnI GATC 2 cut(s) 435, 733
DpnII GATC 2 cut(s) 433, 731
EaeI YGGCCR 1 cut(s) 936
Eam1104I CTCTTC 2 cut(s) 512, 1064
EarI CTCTTC 2 cut(s) 512, 1064
Eco130I CCWWGG 2 cut(s) 90, 688
Eco31I GGTCTC 1 cut(s) 698
Eco47I GGWCC 1 cut(s) 698
EcoRII CCWGG 3 cut(s) 206, 663, 829
EcoT14I CCWWGG 2 cut(s) 90, 688
EcoT22I ATGCAT 2 cut(s) 370, 1015
ErhI CCWWGG 2 cut(s) 90, 688
Esp3I CGTCTC 1 cut(s) 858
FaeI CATG 2 cut(s) 94, 692
FaqI GGGAC 2 cut(s) 546, 654
FatI CATG 2 cut(s) 90, 688
FbaI TGATCA 1 cut(s) 433
FblI GTMKAC 1 cut(s) 235
Fnu4HI GCNGC 7 cut(s) 114, 495, 498, 709, 982, 1032, 1041
FokI GGATG 2 cut(s) 73, 1022
Fsp4HI GCNGC 7 cut(s) 114, 495, 498, 709, 982, 1032, 1041
FspBI CTAG 1 cut(s) 1138
GluI GCNGC 7 cut(s) 114, 495, 498, 709, 982, 1032, 1041
HaeIII GGCC 1 cut(s) 938
HapII CCGG 3 cut(s) 988, 1086, 1116
HgaI GACGC 2 cut(s) 661, 1037
Hin1I GRCGYC 1 cut(s) 867
Hin1II CATG 2 cut(s) 94, 692
HincII GTYRAC 1 cut(s) 236
HindII GTYRAC 1 cut(s) 236
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 4 cut(s) 143, 298, 402, 726
HpaII CCGG 3 cut(s) 988, 1086, 1116
HphI GGTGA 2 cut(s) 152, 1125
Hpy166II GTNNAC 4 cut(s) 236, 597, 763, 1121
Hpy188I TCNGA 4 cut(s) 286, 417, 698, 731
Hpy188III TCNNGA 2 cut(s) 602, 1063
Hpy8I GTNNAC 4 cut(s) 236, 597, 763, 1121
Hpy99I CGWCG 1 cut(s) 1031
HpyAV CCTTC 7 cut(s) 114, 139, 726, 929, 1092, 1122, 1140
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 3 cut(s) 683, 867, 999
HpyF10VI GCNNNNNNNGC 5 cut(s) 494, 961, 1037, 1040, 1046
HpyF3I CTNAG 3 cut(s) 422, 477, 634
HpySE526I ACGT 3 cut(s) 683, 867, 999
Hsp92I GRCGYC 1 cut(s) 867
Hsp92II CATG 2 cut(s) 94, 692
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 2 cut(s) 433, 731
LguI GCTCTTC 1 cut(s) 512
LmnI GCTCC 4 cut(s) 377, 540, 807, 1114
Lsp1109I GCAGC 6 cut(s) 125, 481, 509, 695, 993, 1043
LweI GCATC 6 cut(s) 331, 377, 615, 762, 1000, 1100
MaeI CTAG 1 cut(s) 1138
MaeII ACGT 3 cut(s) 683, 867, 999
MaeIII GTNAC 4 cut(s) 49, 124, 140, 327
MalI GATC 2 cut(s) 435, 733
MboI GATC 2 cut(s) 433, 731
MboII GAAGA 6 cut(s) 127, 273, 499, 514, 517, 1051
MhlI GDGCHC 3 cut(s) 490, 673, 1123
MlsI TGGCCA 1 cut(s) 938
MluCI AATT 9 cut(s) 21, 199, 361, 445, 559, 608, 617, 779, 967
MluNI TGGCCA 1 cut(s) 938
MlyI GAGTC 3 cut(s) 137, 396, 735
MnlI CCTC 7 cut(s) 240, 411, 631, 820, 881, 944, 1089
Mox20I TGGCCA 1 cut(s) 938
Mph1103I ATGCAT 2 cut(s) 370, 1015
MroXI GAANNNNTTC 1 cut(s) 1070
MscI TGGCCA 1 cut(s) 938
MseI TTAA 7 cut(s) 12, 278, 351, 648, 752, 878, 906
MslI CAYNNNNRTG 3 cut(s) 89, 666, 976
Msp20I TGGCCA 1 cut(s) 938
MspCI CTTAAG 1 cut(s) 647
MspI CCGG 3 cut(s) 988, 1086, 1116
MspR9I CCNGG 3 cut(s) 208, 665, 831
Mva1269I GAATGC 2 cut(s) 711, 1013
MvaI CCWGG 3 cut(s) 208, 665, 831
MvnI CGCG 1 cut(s) 1040
MwoI GCNNNNNNNGC 5 cut(s) 494, 961, 1037, 1040, 1046
NcoI CCATGG 2 cut(s) 90, 688
NdeII GATC 2 cut(s) 433, 731
NlaIII CATG 2 cut(s) 94, 692
NlaIV GGNNCC 2 cut(s) 670, 1110
NmuCI GTSAC 4 cut(s) 49, 124, 140, 327
NsiI ATGCAT 2 cut(s) 370, 1015
NspV TTCGAA 1 cut(s) 914
OliI CACNNNNGTG 1 cut(s) 666
PasI CCCWGGG 1 cut(s) 830
PciSI GCTCTTC 1 cut(s) 512
PcsI WCGNNNNNNNCGW 1 cut(s) 920
PctI GAATGC 2 cut(s) 711, 1013
PdmI GAANNNNTTC 1 cut(s) 1070
PfeI GAWTC 1 cut(s) 298
PkrI GCNGC 7 cut(s) 115, 496, 499, 710, 983, 1033, 1042
PleI GAGTC 3 cut(s) 137, 396, 734
PpsI GAGTC 3 cut(s) 137, 396, 734
Ppu21I YACGTR 1 cut(s) 1000
PshAI GACNNNNGTC 1 cut(s) 131
PshBI ATTAAT 1 cut(s) 12
Psp6I CCWGG 3 cut(s) 206, 663, 829
PspGI CCWGG 3 cut(s) 206, 663, 829
PspN4I GGNNCC 2 cut(s) 670, 1110
PspPI GGNCC 1 cut(s) 698
PstI CTGCAG 2 cut(s) 180, 499
PstNI CAGNNNCTG 1 cut(s) 421
RsaI GTAC 3 cut(s) 596, 631, 998
RsaNI GTAC 3 cut(s) 595, 630, 997
RseI CAYNNNNRTG 3 cut(s) 89, 666, 976
SalI GTCGAC 1 cut(s) 234
SapI GCTCTTC 1 cut(s) 512
SaqAI TTAA 7 cut(s) 12, 278, 351, 648, 752, 878, 906
SatI GCNGC 7 cut(s) 114, 495, 498, 709, 982, 1032, 1041
Sau3AI GATC 2 cut(s) 433, 731
Sau96I GGNCC 1 cut(s) 698
SchI GAGTC 3 cut(s) 137, 396, 735
ScrFI CCNGG 3 cut(s) 208, 665, 831
SduI GDGCHC 3 cut(s) 490, 673, 1123
SfaNI GCATC 6 cut(s) 331, 377, 615, 762, 1000, 1100
SfcI CTRYAG 3 cut(s) 176, 495, 891
SfuI TTCGAA 1 cut(s) 914
SinI GGWCC 1 cut(s) 698
SmiMI CAYNNNNRTG 3 cut(s) 89, 666, 976
SmlI CTYRAG 1 cut(s) 647
SmoI CTYRAG 1 cut(s) 647
Sse9I AATT 9 cut(s) 21, 199, 361, 445, 559, 608, 617, 779, 967
SsiI CCGC 1 cut(s) 1040
SspMI CTAG 1 cut(s) 1138
StyD4I CCNGG 3 cut(s) 206, 663, 829
StyI CCWWGG 2 cut(s) 90, 688
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 3 cut(s) 686, 870, 1002
TaqI TCGA 5 cut(s) 18, 219, 235, 873, 914
TasI AATT 9 cut(s) 21, 199, 361, 445, 559, 608, 617, 779, 967
TatI WGTACW 1 cut(s) 594
TauI GCSGC 1 cut(s) 1043
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 7 cut(s) 12, 278, 351, 648, 752, 878, 906
Tru9I TTAA 7 cut(s) 12, 278, 351, 648, 752, 878, 906
TscAI CASTG 3 cut(s) 351, 403, 752
TseFI GTSAC 4 cut(s) 49, 124, 140, 327
TseI GCWGC 6 cut(s) 113, 494, 497, 708, 981, 1031
Tsp45I GTSAC 4 cut(s) 49, 124, 140, 327
TspDTI ATGAA 1 cut(s) 96
TspGWI ACGGA 4 cut(s) 346, 476, 876, 912
TspRI CASTG 3 cut(s) 351, 403, 752
Vha464I CTTAAG 1 cut(s) 647
VneI GTGCAC 1 cut(s) 1119
VpaK11BI GGWCC 1 cut(s) 698
VspI ATTAAT 1 cut(s) 12
XapI RAATTY 2 cut(s) 21, 445
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XmiI GTMKAC 1 cut(s) 235
XmnI GAANNNNTTC 1 cut(s) 1070
XspI CTAG 1 cut(s) 1138
ZraI GACGTC 1 cut(s) 868
Zsp2I ATGCAT 2 cut(s) 370, 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.