Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr17

Orthogroup ID

OG0006185

Physical Location & Seq

Genomic Coordinates Reverse (-)

26074346 .. 26076719

Gene Structure 2374 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGATGTGTCTCACGGCATGTTGAGTGAGAGGACGTTGTATCACATTGCCGCCCACGTGAAATTAATTAAGAGATGCTCCTCGAACCCTCAGGCTGCGAGTCTCAAACCGTGTTCGACTCTCCCTCCACAGTCCACAGTCCACTGTCCAAAGACTCCAAGCCCCCAAACCAAGAGAGAGAATCTATTCCCTTGTTTTCTCCCGATGGAAAGGGAGCCTTTTCTCGGCCCCCAAACAGGGCCCCAACACCAACTCCTCCGATCCACCCCTACCCCCACTGTTCAAGTCCCCGACCCTGGTGATTTCTCTTTCAAAATCTCTCCCTCCCACAGCGAGCCTAATCTCCACCACTTCCAACAACCCCACCATCACCATGTCCACCAACACGACCCGCAGCCCTCTTCGTCCGGACTCCCGAATGCCCACTATCAGAAGAGACCCGGCACTCTCCACCGCTGCAGGACGGCCCCCGCCATGGCCATTATGCAAGGCCTCAATCCAAACCCAACAACCCAGGTCCCCAAACCACAATCCGACTCCACCTCCATCATCAGGCAAGCCGTCTTCTTGCTCCTCGTCTACCTATCCCTCGGCGTTGTAATTTACTCCTTCAGCAGGGACAAATTCTCCGGCGTCGAAACCCACCCGGTCGTCGACGCCCTCTACTTCTGTATAGTCACCATGTGCACCATTGGGTACGGTGACATTGCTCCGACCACTCCTTTCACCAAGATTTTTGCTTGCATCTTTGTGCTTTTCGGGTTCGGTTTCATCGACATTTTGCTCAGTGGGGTCGTCAATTTTGTCCTCGACTTGCAAGAAAACATGATCTTGACCGGAATTCAAATGGGTCAGACTTCCCGGAATCATGGCTTCTCCGCCAGGGACTATATTGTCGATGTGGCGAAGGGGAGGATGAGAATCCGACTCAAGGTCGGCCTGGCGCTTGGGGTGGTGGTTCTGTGCATCGGCATTGGGGCTTTGGTGTTATGTTTTGTGGAGAATTTGGACTGGATTGATTCTGTTTACTTGTCCGTTATGTCGGTTACGACGGTTGGGTACGGTGACAGGGCCTTCAAGACTCTCCAAGGGAGACTCTTCGCCGCCGTGTGGCTTCTGTTTTCGACGTTGGCGGTGGCTCGGGCGTTTATATATTTAGCAGAGGCCAGGGTGGACAAAAGGCACAGGAGGATTATGAACTGGGTTTTGCATAGGGATATCACTGTCCAGGATCTGCTTGCCGCCGACATCAATAACCAAGGTTTCATCAGTAAGTCAGAGTATGTTATTTACAAGCTTAAAGAGATGGGAAAGATAGGGGAGAAAGATATTCTGCAAATCTGCGATCAGTTCAGTAAGCTTGACCAGAACCACTCTGGGAAGATAACACTGCCTGATCTGTTGGCGCATCGCTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005216 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009705 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022838 GO:0022840 GO:0022841 GO:0022842 GO:0022857 GO:0022890 GO:0030001 GO:0030322 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ion_trans_2

Protein Analysis

473

Amino Acids

52.68

Weight (kDa)

9.04

Isoelectric Point (pI)

33.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans_2	PF07885	190 - 269	1.4e-15	Ion channel
Ion_trans_2	PF07885	319 - 387	6.8e-12	Ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006185)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01840
fragaria_vesca	FvH4_1g26310 FvH4_1g26310
malus_domestica	MD09G1289500.v1.1 MD17G1284200.v1.1
prunus_persica	Prupe.3G003200_v2.0.a1 Prupe.3G003200_v2.0.a1 Prupe.5G044500_v2.0.a1
pyrus_communis	pycom09g19750 pycom14g08130 pycom17g28020
rosa_chinensis	RchiOBHm_Chr1g0333551 RchiOBHm_Chr3g0485981
rosa_laevigata	RLG00000023117 RLG00000035326
rosa_multiflora	Rmu_sc0003151.1_g000035 Rmu_sc0004032.1_g000015 Rmu_sc0009687.1_g000001 Rmu_sc0041532.1_g000001
rosa_roxburghii	Rroxscaffold_6G00396140
rosa_rugosa	Rorug03G0223100
rosa_samantha	Rh3AG272700 Rh3BG307400 Rh3CG306000 Rh3DG302300
rosa_wichuraiana	Rw3G024180 Rw6G027690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	893
AccI	GTMKAC	2 cut(s)	579, 654
AccIII	TCCGGA	1 cut(s)	407
AciI	CCGC	8 cut(s)	51, 392, 454, 471, 879, 1104, 1133, 1242
AclWI	GGATC	2 cut(s)	255, 1239
AcoI	YGGCCR	1 cut(s)	477
AcsI	RAATTY	3 cut(s)	623, 840, 1003
AcuI	CTGAAG	1 cut(s)	595
AcvI	CACGTG	1 cut(s)	58
AcyI	GRCGYC	2 cut(s)	633, 657
AdeI	CACNNNGTG	1 cut(s)	684
AfaI	GTAC	2 cut(s)	698, 1061
AfiI	CCNNNNNNNGG	9 cut(s)	224, 236, 237, 296, 475, 552, 615, 931, 1110
AgsI	TTSAA	4 cut(s)	284, 313, 845, 1078
AhdI	GACNNNNNGTC	1 cut(s)	932
AjnI	CCWGG	6 cut(s)	295, 513, 881, 939, 1166, 1227
AluBI	AGCT	2 cut(s)	1297, 1360
AluI	AGCT	2 cut(s)	1297, 1360
Alw21I	GWGCWC	1 cut(s)	689
Alw26I	GTCTC	4 cut(s)	15, 107, 430, 1087
Alw44I	GTGCAC	1 cut(s)	685
AlwI	GGATC	2 cut(s)	255, 1239
AlwNI	CAGNNNCTG	1 cut(s)	1234
Ama87I	CYCGRG	1 cut(s)	1140
Aor13HI	TCCGGA	1 cut(s)	407
AoxI	GGCC	8 cut(s)	226, 239, 465, 477, 490, 937, 1071, 1164
ApaI	GGGCCC	1 cut(s)	243
ApaLI	GTGCAC	1 cut(s)	685
ApeKI	GCWGC	3 cut(s)	95, 394, 456
ApoI	RAATTY	3 cut(s)	623, 840, 1003
AseI	ATTAAT	1 cut(s)	65
Asp700I	GAANNNNTTC	1 cut(s)	185
AspLEI	GCGC	2 cut(s)	946, 1408
AspS9I	GGNCC	6 cut(s)	227, 239, 240, 466, 517, 1071
AsuC2I	CCSGG	3 cut(s)	441, 647, 862
AsuHPI	GGTGA	6 cut(s)	311, 362, 670, 713, 718, 1076
AvaI	CYCGRG	1 cut(s)	1140
AvaII	GGWCC	1 cut(s)	517
AxyI	CCTNAGG	1 cut(s)	90
BaeGI	GKGCMC	2 cut(s)	243, 689
BalI	TGGCCA	1 cut(s)	479
BanII	GRGCYC	1 cut(s)	243
BbrPI	CACGTG	1 cut(s)	58
BbsI	GAAGAC	1 cut(s)	556
Bbv12I	GWGCWC	1 cut(s)	689
BbvI	GCAGC	3 cut(s)	82, 406, 443
BccI	CCATC	4 cut(s)	199, 375, 554, 1300
BceAI	ACGGC	4 cut(s)	31, 480, 545, 1091
BciT130I	CCWGG	6 cut(s)	297, 515, 883, 941, 1168, 1229
BcnI	CCSGG	3 cut(s)	441, 647, 862
BcoDI	GTCTC	4 cut(s)	15, 107, 430, 1087
BfmI	CTRYAG	1 cut(s)	457
BfoI	RGCGCY	1 cut(s)	947
BisI	GCNGC	6 cut(s)	51, 96, 395, 457, 1104, 1242
BlsI	GCNGC	6 cut(s)	52, 97, 396, 458, 1105, 1243
Bme1390I	CCNGG	9 cut(s)	297, 441, 515, 647, 862, 883, 941, 1168, 1229
Bme18I	GGWCC	1 cut(s)	517
BmeRI	GACNNNNNGTC	1 cut(s)	932
BmeT110I	CYCGRG	1 cut(s)	1140
BmgT120I	GGNCC	6 cut(s)	227, 239, 240, 466, 517, 1071
BmiI	GGNNCC	6 cut(s)	216, 229, 241, 242, 468, 519
BmrFI	CCNGG	9 cut(s)	297, 441, 515, 647, 862, 883, 941, 1168, 1229
BmrI	ACTGGG	1 cut(s)	1210
BmsI	GCATC	3 cut(s)	65, 753, 975
BmuI	ACTGGG	1 cut(s)	1210
BpiI	GAAGAC	1 cut(s)	556
BpuEI	CTTGAG	1 cut(s)	914
BpuMI	CCSGG	3 cut(s)	441, 647, 862
BsaAI	YACGTR	1 cut(s)	58
BsaBI	GATNNNNATC	1 cut(s)	920
BsaHI	GRCGYC	2 cut(s)	633, 657
BsaI	GGTCTC	1 cut(s)	430
BsaJI	CCNNGG	8 cut(s)	295, 474, 513, 589, 882, 1087, 1167, 1258
BsaWI	WCCGGW	2 cut(s)	407, 836
BsaXI	ACNNNNNCTCC	8 cut(s)	109, 139, 237, 267, 527, 557, 611, 641
Bsc4I	CCNNNNNNNGG	9 cut(s)	224, 236, 237, 296, 475, 552, 615, 931, 1110
Bse1I	ACTGG	2 cut(s)	1016, 1205
Bse21I	CCTNAGG	1 cut(s)	90
Bse3DI	GCAATG	2 cut(s)	45, 705
Bse8I	GATNNNNATC	1 cut(s)	920
BseAI	TCCGGA	1 cut(s)	407
BseBI	CCWGG	6 cut(s)	297, 515, 883, 941, 1168, 1229
BseDI	CCNNGG	8 cut(s)	295, 474, 513, 589, 882, 1087, 1167, 1258
BseGI	GGATG	2 cut(s)	10, 921
BseJI	GATNNNNATC	1 cut(s)	920
BseLI	CCNNNNNNNGG	9 cut(s)	224, 236, 237, 296, 475, 552, 615, 931, 1110
BseMI	GCAATG	2 cut(s)	45, 705
BseMII	CTCAG	2 cut(s)	104, 799
BseNI	ACTGG	2 cut(s)	1016, 1205
BseRI	GAGGAG	3 cut(s)	70, 245, 563
BseSI	GKGCMC	2 cut(s)	243, 689
BseXI	GCAGC	3 cut(s)	82, 406, 443
Bsh1285I	CGRYCG	1 cut(s)	651
BshFI	GGCC	8 cut(s)	228, 241, 467, 479, 492, 939, 1073, 1166
BsiEI	CGRYCG	1 cut(s)	651
BsiHKAI	GWGCWC	1 cut(s)	689
BsiHKCI	CYCGRG	1 cut(s)	1140
BsiSI	CCGG	6 cut(s)	408, 441, 630, 647, 837, 862
BslFI	GGGAC	4 cut(s)	272, 503, 632, 899
BslI	CCNNNNNNNGG	9 cut(s)	224, 236, 237, 296, 475, 552, 615, 931, 1110
BsmAI	GTCTC	4 cut(s)	15, 107, 430, 1087
BsmFI	GGGAC	4 cut(s)	272, 503, 632, 899
BsmI	GAATGC	1 cut(s)	424
BsnI	GGCC	8 cut(s)	228, 241, 467, 479, 492, 939, 1073, 1166
Bso31I	GGTCTC	1 cut(s)	430
BsoBI	CYCGRG	1 cut(s)	1140
Bsp120I	GGGCCC	1 cut(s)	239
Bsp1286I	GDGCHC	2 cut(s)	243, 689
Bsp13I	TCCGGA	1 cut(s)	407
Bsp143I	GATC	5 cut(s)	260, 828, 1231, 1345, 1396
Bsp19I	CCATGG	1 cut(s)	474
BspACI	CCGC	8 cut(s)	51, 392, 454, 471, 879, 1104, 1133, 1242
BspANI	GGCC	8 cut(s)	228, 241, 467, 479, 492, 939, 1073, 1166
BspCNI	CTCAG	2 cut(s)	103, 798
BspEI	TCCGGA	1 cut(s)	407
BspLI	GGNNCC	6 cut(s)	216, 229, 241, 242, 468, 519
BspMAI	CTGCAG	1 cut(s)	461
BspPI	GGATC	2 cut(s)	255, 1239
BspTNI	GGTCTC	1 cut(s)	430
BsrDI	GCAATG	2 cut(s)	45, 705
BsrI	ACTGG	2 cut(s)	1016, 1205
BssECI	CCNNGG	8 cut(s)	295, 474, 513, 589, 882, 1087, 1167, 1258
BssMI	GATC	5 cut(s)	260, 828, 1231, 1345, 1396
BssNI	GRCGYC	2 cut(s)	633, 657
BssT1I	CCWWGG	3 cut(s)	474, 1087, 1258
Bst2UI	CCWGG	6 cut(s)	297, 515, 883, 941, 1168, 1229
Bst4CI	ACNGT	9 cut(s)	111, 132, 139, 146, 280, 701, 1054, 1064, 1225
Bst6I	CTCTTC	3 cut(s)	406, 428, 1103
BstACI	GRCGYC	2 cut(s)	633, 657
BstBAI	YACGTR	1 cut(s)	58
BstC8I	GCNNGC	4 cut(s)	335, 558, 742, 1239
BstDEI	CTNAG	2 cut(s)	90, 785
BstDSI	CCRYGG	1 cut(s)	474
BstENI	CCTNNNNNAGG	1 cut(s)	613
BstF5I	GGATG	2 cut(s)	10, 921
BstH2I	RGCGCY	1 cut(s)	947
BstHHI	GCGC	2 cut(s)	946, 1408
BstKTI	GATC	5 cut(s)	263, 831, 1234, 1348, 1399
BstMAI	GTCTC	4 cut(s)	15, 107, 430, 1087
BstMBI	GATC	5 cut(s)	260, 828, 1231, 1345, 1396
BstMCI	CGRYCG	1 cut(s)	651
BstNI	CCWGG	6 cut(s)	297, 515, 883, 941, 1168, 1229
BstNSI	RCATGY	1 cut(s)	22
BstSCI	CCNGG	9 cut(s)	295, 439, 513, 645, 860, 881, 939, 1166, 1227
BstSFI	CTRYAG	1 cut(s)	457
BstSLI	GKGCMC	2 cut(s)	243, 689
BstV1I	GCAGC	3 cut(s)	82, 406, 443
BstV2I	GAAGAC	1 cut(s)	556
BstX2I	RGATCY	1 cut(s)	1231
BstYI	RGATCY	1 cut(s)	1231
Bsu36I	CCTNAGG	1 cut(s)	90
BsuRI	GGCC	8 cut(s)	228, 241, 467, 479, 492, 939, 1073, 1166
BtgI	CCRYGG	1 cut(s)	474
BtgZI	GCGATG	1 cut(s)	1394
BtsCI	GGATG	2 cut(s)	10, 921
BtsI	GCAGTG	1 cut(s)	1388
BtsIMutI	CAGTG	5 cut(s)	142, 276, 793, 1221, 1388
Cac8I	GCNNGC	4 cut(s)	335, 558, 742, 1239
CaiI	CAGNNNCTG	1 cut(s)	1234
CfoI	GCGC	2 cut(s)	946, 1408
Cfr13I	GGNCC	6 cut(s)	227, 239, 240, 466, 517, 1071
CseI	GACGC	2 cut(s)	622, 665
Csp6I	GTAC	2 cut(s)	697, 1060
CviAII	CATG	6 cut(s)	19, 374, 475, 682, 826, 869
CviQI	GTAC	2 cut(s)	697, 1060
DdeI	CTNAG	2 cut(s)	90, 785
DpnI	GATC	5 cut(s)	262, 830, 1233, 1347, 1398
DpnII	GATC	5 cut(s)	260, 828, 1231, 1345, 1396
DraIII	CACNNNGTG	1 cut(s)	684
DrdI	GACNNNNNNGTC	1 cut(s)	893
DriI	GACNNNNNGTC	1 cut(s)	932
DseDI	GACNNNNNNGTC	1 cut(s)	893
EaeI	YGGCCR	1 cut(s)	477
Eam1104I	CTCTTC	3 cut(s)	406, 428, 1103
Eam1105I	GACNNNNNGTC	1 cut(s)	932
EarI	CTCTTC	3 cut(s)	406, 428, 1103
EciI	GGCGGA	1 cut(s)	868
Eco130I	CCWWGG	3 cut(s)	474, 1087, 1258
Eco147I	AGGCCT	1 cut(s)	492
Eco24I	GRGCYC	1 cut(s)	243
Eco31I	GGTCTC	1 cut(s)	430
Eco32I	GATATC	1 cut(s)	1219
Eco47I	GGWCC	1 cut(s)	517
Eco57I	CTGAAG	1 cut(s)	595
Eco72I	CACGTG	1 cut(s)	58
Eco81I	CCTNAGG	1 cut(s)	90
Eco88I	CYCGRG	1 cut(s)	1140
EcoNI	CCTNNNNNAGG	1 cut(s)	613
EcoO109I	RGGNCCY	4 cut(s)	239, 240, 517, 1071
EcoRI	GAATTC	1 cut(s)	840
EcoRII	CCWGG	6 cut(s)	295, 513, 881, 939, 1166, 1227
EcoRV	GATATC	1 cut(s)	1219
EcoT14I	CCWWGG	3 cut(s)	474, 1087, 1258
EcoT38I	GRGCYC	1 cut(s)	243
ErhI	CCWWGG	3 cut(s)	474, 1087, 1258
FaeI	CATG	6 cut(s)	22, 377, 478, 685, 829, 872
FalI	AAGNNNNNCTT	2 cut(s)	202, 234
FaqI	GGGAC	4 cut(s)	272, 503, 632, 899
FatI	CATG	6 cut(s)	18, 373, 474, 681, 825, 868
FauI	CCCGC	2 cut(s)	399, 478
FblI	GTMKAC	2 cut(s)	579, 654
Fnu4HI	GCNGC	6 cut(s)	51, 96, 395, 457, 1104, 1242
FokI	GGATG	2 cut(s)	17, 928
FriOI	GRGCYC	1 cut(s)	243
Fsp4HI	GCNGC	6 cut(s)	51, 96, 395, 457, 1104, 1242
GlaI	GCGC	2 cut(s)	945, 1407
GluI	GCNGC	6 cut(s)	51, 96, 395, 457, 1104, 1242
HaeII	RGCGCY	1 cut(s)	947
HaeIII	GGCC	8 cut(s)	228, 241, 467, 479, 492, 939, 1073, 1166
HapII	CCGG	6 cut(s)	408, 441, 630, 647, 837, 862
HgaI	GACGC	2 cut(s)	622, 665
HhaI	GCGC	2 cut(s)	946, 1408
Hin1I	GRCGYC	2 cut(s)	633, 657
Hin1II	CATG	6 cut(s)	22, 377, 478, 685, 829, 872
Hin6I	GCGC	2 cut(s)	944, 1406
HinP1I	GCGC	2 cut(s)	944, 1406
HincII	GTYRAC	1 cut(s)	655
HindII	GTYRAC	1 cut(s)	655
HindIII	AAGCTT	2 cut(s)	1295, 1358
HpaII	CCGG	6 cut(s)	408, 441, 630, 647, 837, 862
HphI	GGTGA	6 cut(s)	311, 362, 670, 713, 718, 1076
Hpy166II	GTNNAC	8 cut(s)	135, 142, 379, 580, 655, 687, 1027, 1174
Hpy188I	TCNGA	7 cut(s)	260, 432, 535, 714, 855, 926, 1279
Hpy188III	TCNNGA	5 cut(s)	202, 408, 415, 832, 1078
Hpy8I	GTNNAC	8 cut(s)	135, 142, 379, 580, 655, 687, 1027, 1174
Hpy99I	CGWCG	5 cut(s)	638, 656, 659, 1054, 1129
HpyAV	CCTTC	3 cut(s)	619, 901, 1084
HpyCH4III	ACNGT	9 cut(s)	111, 132, 139, 146, 280, 701, 1054, 1064, 1225
HpyCH4IV	ACGT	3 cut(s)	35, 57, 1127
HpyCH4V	TGCA	8 cut(s)	459, 487, 687, 744, 817, 966, 1210, 1336
HpyF3I	CTNAG	2 cut(s)	90, 785
HpySE526I	ACGT	3 cut(s)	35, 57, 1127
Hsp92I	GRCGYC	2 cut(s)	633, 657
Hsp92II	CATG	6 cut(s)	22, 377, 478, 685, 829, 872
HspAI	GCGC	2 cut(s)	944, 1406
Kpn2I	TCCGGA	1 cut(s)	407
Kzo9I	GATC	5 cut(s)	260, 828, 1231, 1345, 1396
LmnI	GCTCC	4 cut(s)	83, 214, 576, 715
Lsp1109I	GCAGC	3 cut(s)	82, 406, 443
LweI	GCATC	3 cut(s)	65, 753, 975
MaeII	ACGT	3 cut(s)	35, 57, 1127
MaeIII	GTNAC	4 cut(s)	676, 701, 1045, 1064
MalI	GATC	5 cut(s)	262, 830, 1233, 1347, 1398
MboI	GATC	5 cut(s)	260, 828, 1231, 1345, 1396
MboII	GAAGA	5 cut(s)	393, 445, 556, 1090, 1393
MflI	RGATCY	1 cut(s)	1231
MhlI	GDGCHC	2 cut(s)	243, 689
MlsI	TGGCCA	1 cut(s)	479
MluCI	AATT	7 cut(s)	62, 66, 600, 623, 799, 840, 1003
MluNI	TGGCCA	1 cut(s)	479
MlyI	GAGTC	8 cut(s)	109, 112, 148, 405, 530, 921, 1075, 1089
MmeI	TCCRAC	4 cut(s)	379, 558, 737, 949
Mox20I	TGGCCA	1 cut(s)	479
MroI	TCCGGA	1 cut(s)	407
MroXI	GAANNNNTTC	1 cut(s)	185
MscI	TGGCCA	1 cut(s)	479
MseI	TTAA	3 cut(s)	65, 69, 1299
MslI	CAYNNNNRTG	2 cut(s)	372, 749
Msp20I	TGGCCA	1 cut(s)	479
MspA1I	CMGCKG	1 cut(s)	456
MspI	CCGG	6 cut(s)	408, 441, 630, 647, 837, 862
MspR9I	CCNGG	9 cut(s)	297, 441, 515, 647, 862, 883, 941, 1168, 1229
Mva1269I	GAATGC	1 cut(s)	424
MvaI	CCWGG	6 cut(s)	297, 515, 883, 941, 1168, 1229
NciI	CCSGG	3 cut(s)	441, 647, 862
NcoI	CCATGG	1 cut(s)	474
NdeII	GATC	5 cut(s)	260, 828, 1231, 1345, 1396
NlaIII	CATG	6 cut(s)	22, 377, 478, 685, 829, 872
NlaIV	GGNNCC	6 cut(s)	216, 229, 241, 242, 468, 519
NmeAIII	GCCGAG	2 cut(s)	204, 570
NmuCI	GTSAC	3 cut(s)	676, 701, 1064
NspI	RCATGY	1 cut(s)	22
PacI	TTAATTAA	1 cut(s)	69
PceI	AGGCCT	1 cut(s)	492
PcsI	WCGNNNNNNNCGW	1 cut(s)	771
PctI	GAATGC	1 cut(s)	424
PdmI	GAANNNNTTC	1 cut(s)	185
PfeI	GAWTC	4 cut(s)	181, 865, 921, 1019
PfoI	TCCNGGA	2 cut(s)	860, 1227
PkrI	GCNGC	6 cut(s)	52, 97, 396, 458, 1105, 1243
PleI	GAGTC	8 cut(s)	108, 112, 148, 405, 530, 921, 1075, 1089
PmaCI	CACGTG	1 cut(s)	58
PmlI	CACGTG	1 cut(s)	58
PpsI	GAGTC	8 cut(s)	108, 112, 148, 405, 530, 921, 1075, 1089
Ppu21I	YACGTR	1 cut(s)	58
PpuMI	RGGWCCY	1 cut(s)	517
PshBI	ATTAAT	1 cut(s)	65
Psp5II	RGGWCCY	1 cut(s)	517
Psp6I	CCWGG	6 cut(s)	295, 513, 881, 939, 1166, 1227
PspCI	CACGTG	1 cut(s)	58
PspGI	CCWGG	6 cut(s)	295, 513, 881, 939, 1166, 1227
PspN4I	GGNNCC	6 cut(s)	216, 229, 241, 242, 468, 519
PspOMI	GGGCCC	1 cut(s)	239
PspPI	GGNCC	6 cut(s)	227, 239, 240, 466, 517, 1071
PspPPI	RGGWCCY	1 cut(s)	517
PstI	CTGCAG	1 cut(s)	461
PstNI	CAGNNNCTG	1 cut(s)	1234
PsuI	RGATCY	1 cut(s)	1231
RsaI	GTAC	2 cut(s)	698, 1061
RsaNI	GTAC	2 cut(s)	697, 1060
RseI	CAYNNNNRTG	2 cut(s)	372, 749
SalI	GTCGAC	1 cut(s)	653
SaqAI	TTAA	3 cut(s)	65, 69, 1299
SatI	GCNGC	6 cut(s)	51, 96, 395, 457, 1104, 1242
Sau3AI	GATC	5 cut(s)	260, 828, 1231, 1345, 1396
Sau96I	GGNCC	6 cut(s)	227, 239, 240, 466, 517, 1071
SchI	GAGTC	8 cut(s)	109, 112, 148, 405, 530, 921, 1075, 1089
ScrFI	CCNGG	9 cut(s)	297, 441, 515, 647, 862, 883, 941, 1168, 1229
SduI	GDGCHC	2 cut(s)	243, 689
SfaNI	GCATC	3 cut(s)	65, 753, 975
SfcI	CTRYAG	1 cut(s)	457
SgrDI	CGTCGACG	1 cut(s)	653
SinI	GGWCC	1 cut(s)	517
SmiMI	CAYNNNNRTG	2 cut(s)	372, 749
SmlI	CTYRAG	1 cut(s)	929
SmoI	CTYRAG	1 cut(s)	929
Sse9I	AATT	7 cut(s)	62, 66, 600, 623, 799, 840, 1003
SseBI	AGGCCT	1 cut(s)	492
SsiI	CCGC	8 cut(s)	51, 392, 454, 471, 879, 1104, 1133, 1242
StuI	AGGCCT	1 cut(s)	492
StyD4I	CCNGG	9 cut(s)	295, 439, 513, 645, 860, 881, 939, 1166, 1227
StyI	CCWWGG	3 cut(s)	474, 1087, 1258
TaaI	ACNGT	9 cut(s)	111, 132, 139, 146, 280, 701, 1054, 1064, 1225
TaiI	ACGT	3 cut(s)	38, 60, 1130
TaqI	TCGA	8 cut(s)	83, 116, 636, 654, 774, 810, 897, 1124
TasI	AATT	7 cut(s)	62, 66, 600, 623, 799, 840, 1003
TauI	GCSGC	3 cut(s)	53, 1106, 1244
TfiI	GAWTC	4 cut(s)	181, 865, 921, 1019
Tru1I	TTAA	3 cut(s)	65, 69, 1299
Tru9I	TTAA	3 cut(s)	65, 69, 1299
TscAI	CASTG	5 cut(s)	149, 283, 793, 1228, 1395
TseFI	GTSAC	3 cut(s)	676, 701, 1064
TseI	GCWGC	3 cut(s)	95, 394, 456
Tsp45I	GTSAC	3 cut(s)	676, 701, 1064
TspDTI	ATGAA	3 cut(s)	760, 1211, 1255
TspGWI	ACGGA	1 cut(s)	1024
TspRI	CASTG	5 cut(s)	149, 283, 793, 1228, 1395
VneI	GTGCAC	1 cut(s)	685
VpaK11BI	GGWCC	1 cut(s)	517
VspI	ATTAAT	1 cut(s)	65
XagI	CCTNNNNNAGG	1 cut(s)	613
XapI	RAATTY	3 cut(s)	623, 840, 1003
XceI	RCATGY	1 cut(s)	22
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1373
XmiI	GTMKAC	2 cut(s)	579, 654
XmnI	GAANNNNTTC	1 cut(s)	185

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.