pycom420g00920

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0013723
Physical Location & Seq
Reverse (-)
1242167 .. 1242784
618 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGGCTAATGTAGAGAAGGTGTTAAGAGTGCCTAAGGAGGATAGACATTTGGGCAAGCTACGGCCTTTGTTTCGAAAGTACGGTTTCCAGCCCCTAGTGTCGGAGTGCCAGAGACTTGCAATAGAGAAAGTGAGCAAGAAAAGGGGGACTAGCACCAAGAAAGGGATAACACCTATGTTAGTTCTCGTAGATGACATTTTGTTTCACAAGGGAGCTCGTAAACACCGGGTGAGGCCAACCCCTAGACCTAAGTCGCAAGAGGAGGTCCTCAAGATTACTGCCTCAAAGAGAGCTGAAGCTGAGGCTATCGGGTATGCTACTGCCATAGTTGCAGGGGAGAAGAGACGACTGTTGCCTTCTCTTCCTACCATCGATCCTATCTTTCCTCCAACCATGGAGTCCACTGGCCAAGAAGGTGACCTTAGCTCCAACCGTAAGAAGAAGTATAAGGAAGAGGTTGGCAGCATCCATAGGAAGGATTTGAAGGTTGCCATGCAGCCAAGTAGTTTTAGGTATGTCAATAATTGCCTGGCAGGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.3

Weight (kDa)

10.17

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 368
AcoI YGGCCR 1 cut(s) 406
AcuI CTGAAG 1 cut(s) 315
AdeI CACNNNGTG 1 cut(s) 229
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 3 cut(s) 100, 162, 475
AgsI TTSAA 1 cut(s) 484
AjnI CCWGG 1 cut(s) 528
AluBI AGCT 5 cut(s) 58, 215, 293, 299, 426
AluI AGCT 5 cut(s) 58, 215, 293, 299, 426
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 2 cut(s) 106, 337
AlwI GGATC 1 cut(s) 368
AoxI GGCC 3 cut(s) 62, 233, 406
ApeKI GCWGC 2 cut(s) 462, 496
AspS9I GGNCC 1 cut(s) 265
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 241, 428
AsuII TTCGAA 1 cut(s) 73
AvaII GGWCC 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 33
BalI TGGCCA 1 cut(s) 408
BanII GRGCYC 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 217
BbvCI CCTCAGC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 474, 508
BccI CCATC 1 cut(s) 377
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 1 cut(s) 530
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 2 cut(s) 106, 337
BfaI CTAG 3 cut(s) 95, 150, 243
BisI GCNGC 2 cut(s) 463, 497
BlsI GCNGC 2 cut(s) 464, 498
Bme1390I CCNGG 2 cut(s) 227, 530
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 265
BmrFI CCNGG 2 cut(s) 227, 530
BmsI GCATC 1 cut(s) 474
BoxI GACNNNNGTC 1 cut(s) 250
Bpu10I CCTNAGC 2 cut(s) 300, 422
Bpu14I TTCGAA 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 227
Bsa29I ATCGAT 1 cut(s) 372
BsaJI CCNNGG 1 cut(s) 393
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 162, 475
Bse1I ACTGG 1 cut(s) 409
Bse21I CCTNAGG 1 cut(s) 33
BseBI CCWGG 1 cut(s) 530
BseCI ATCGAT 1 cut(s) 372
BseDI CCNNGG 1 cut(s) 393
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 3 cut(s) 100, 162, 475
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 2 cut(s) 474, 508
BshFI GGCC 3 cut(s) 64, 235, 408
BshVI ATCGAT 1 cut(s) 372
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 1 cut(s) 226
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 3 cut(s) 100, 162, 475
BsmAI GTCTC 2 cut(s) 106, 337
BsmBI CGTCTC 1 cut(s) 337
BsmFI GGGAC 1 cut(s) 160
BsnI GGCC 3 cut(s) 64, 235, 408
Bsp119I TTCGAA 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 1 cut(s) 373
Bsp19I CCATGG 1 cut(s) 393
BspANI GGCC 3 cut(s) 64, 235, 408
BspCNI CTCAG 1 cut(s) 292
BspDI ATCGAT 1 cut(s) 372
BspPI GGATC 1 cut(s) 368
BspT104I TTCGAA 1 cut(s) 73
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 1 cut(s) 393
BssMI GATC 1 cut(s) 373
BssT1I CCWWGG 1 cut(s) 393
Bst2UI CCWGG 1 cut(s) 530
Bst4CI ACNGT 3 cut(s) 83, 351, 434
Bst6I CTCTTC 3 cut(s) 335, 366, 447
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 4 cut(s) 33, 249, 300, 422
BstDSI CCRYGG 1 cut(s) 393
BstEII GGTNACC 1 cut(s) 416
BstF5I GGATG 1 cut(s) 465
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 2 cut(s) 106, 337
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 1 cut(s) 530
BstPAI GACNNNNGTC 1 cut(s) 250
BstPI GGTNACC 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 225, 528
BstV1I GCAGC 2 cut(s) 474, 508
Bsu15I ATCGAT 1 cut(s) 372
Bsu36I CCTNAGG 1 cut(s) 33
BsuRI GGCC 3 cut(s) 64, 235, 408
BsuTUI ATCGAT 1 cut(s) 372
BtgI CCRYGG 1 cut(s) 393
BtsCI GGATG 1 cut(s) 465
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 265
ClaI ATCGAT 1 cut(s) 372
Csp6I GTAC 1 cut(s) 79
CviAII CATG 2 cut(s) 394, 493
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 4 cut(s) 33, 249, 300, 422
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
DraIII CACNNNGTG 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 3 cut(s) 335, 366, 447
EarI CTCTTC 3 cut(s) 335, 366, 447
Ecl136II GAGCTC 1 cut(s) 215
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 315
Eco81I CCTNAGG 1 cut(s) 33
Eco91I GGTNACC 1 cut(s) 416
EcoICRI GAGCTC 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 265
EcoO65I GGTNACC 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 528
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 393
Esp3I CGTCTC 1 cut(s) 337
FaeI CATG 2 cut(s) 397, 496
FaiI YATR 8 cut(s) 176, 315, 326, 395, 447, 471, 494, 516
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 1 cut(s) 160
FatI CATG 2 cut(s) 393, 492
Fnu4HI GCNGC 2 cut(s) 463, 497
FokI GGATG 1 cut(s) 452
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 2 cut(s) 463, 497
FspBI CTAG 3 cut(s) 95, 150, 243
GluI GCNGC 2 cut(s) 463, 497
HaeIII GGCC 3 cut(s) 64, 235, 408
HapII CCGG 1 cut(s) 226
Hin1II CATG 2 cut(s) 397, 496
HinfI GANTC 1 cut(s) 398
HpaII CCGG 1 cut(s) 226
HphI GGTGA 2 cut(s) 241, 428
Hpy166II GTNNAC 2 cut(s) 221, 402
Hpy188I TCNGA 1 cut(s) 103
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 2 cut(s) 221, 402
HpyAV CCTTC 5 cut(s) 10, 366, 407, 469, 478
HpyCH4III ACNGT 3 cut(s) 83, 351, 434
HpyCH4V TGCA 3 cut(s) 119, 332, 496
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 4 cut(s) 33, 249, 300, 422
Hsp92II CATG 2 cut(s) 397, 496
Kzo9I GATC 1 cut(s) 373
LmnI GCTCC 2 cut(s) 212, 431
LpnPI CCDG 7 cut(s) 101, 122, 239, 318, 390, 515, 519
Lsp1109I GCAGC 2 cut(s) 474, 508
LweI GCATC 1 cut(s) 474
MaeI CTAG 3 cut(s) 95, 150, 243
MaeIII GTNAC 1 cut(s) 416
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 4 cut(s) 352, 353, 451, 464
MhlI GDGCHC 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 1 cut(s) 523
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 3 cut(s) 81, 413, 453
MnlI CCTC 9 cut(s) 31, 225, 253, 256, 278, 292, 295, 396, 448
Mox20I TGGCCA 1 cut(s) 408
MscI TGGCCA 1 cut(s) 408
MseI TTAA 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 408
MspI CCGG 1 cut(s) 226
MspR9I CCNGG 2 cut(s) 227, 530
MvaI CCWGG 1 cut(s) 530
MwoI GCNNNNNNNGC 1 cut(s) 329
NciI CCSGG 1 cut(s) 227
NcoI CCATGG 1 cut(s) 393
NdeII GATC 1 cut(s) 373
NlaIII CATG 2 cut(s) 397, 496
NmuCI GTSAC 1 cut(s) 416
NspV TTCGAA 1 cut(s) 73
PkrI GCNGC 2 cut(s) 464, 498
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
PpuMI RGGWCCY 1 cut(s) 265
PshAI GACNNNNGTC 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 217
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 528
PspEI GGTNACC 1 cut(s) 416
PspGI CCWGG 1 cut(s) 528
PspPI GGNCC 1 cut(s) 265
PspPPI RGGWCCY 1 cut(s) 265
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SacI GAGCTC 1 cut(s) 217
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 2 cut(s) 463, 497
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 2 cut(s) 227, 530
SduI GDGCHC 1 cut(s) 217
SfaNI GCATC 1 cut(s) 474
SfuI TTCGAA 1 cut(s) 73
SinI GGWCC 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 1 cut(s) 523
SspMI CTAG 3 cut(s) 95, 150, 243
SstI GAGCTC 1 cut(s) 217
StyD4I CCNGG 2 cut(s) 225, 528
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 3 cut(s) 83, 351, 434
TaqI TCGA 2 cut(s) 73, 372
TasI AATT 1 cut(s) 523
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TscAI CASTG 1 cut(s) 409
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 2 cut(s) 462, 496
Tsp45I GTSAC 1 cut(s) 416
TspRI CASTG 1 cut(s) 409
VpaK11BI GGWCC 1 cut(s) 265
XspI CTAG 3 cut(s) 95, 150, 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.