pycom520g00130

gag-polypeptide of LTR copia-type

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0010600
Physical Location & Seq
Forward (+)
149233 .. 156911
7679 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5319 bp
ATGCCAAGTGGTTTTATGATTGGATACTCACAAGTGAGTTTCAGAGTTCGGCTTTCTAACGGCCGAACCACATTTACCATCAAGACATACATCTCTTTTGAGTACCTGTGTCCGTATAGTTTGGAGCCGGTTCGACATGCTTATACTTTTACGAATATAATCACCGTGACCGAACCCGACGCCAACGATTCAACCCAAAGGCCGAGACGTCGCGTTGAACGCAGCATCAACAAATTTTACTCCTTGGCTAACGAGTTGGCACGCCCCGCATCAATCGAAGGACGCTTTGTAGTTTTTAGGGTCAACAGTGGGTTCGGCCAAAACCACAAAAGAAATTTTATGTGTTCTTTGTTAATCCTAGATATTAGTCCCCTTTTATTTGAGAGTTTTTGGGTTTTGTTTGTTCTTCATGTTCATAACATGAACAAAGTTTGGGAGTTTTTGATTCAAAATTTTCTAAAAGATTTGTTTGAAATTTGTTTCACACTTTTCACACATCACCATACCAATCACTTTATCAAATTTAAAACTTACACTTCAACAACCCACACACTCCTTACATCACTCCATAACCAGCCATCCTATAAAGTAGGATTTTTGTGTATTTGCACTTTGACCCAAAAGTTTACGTTTTTACGTAAAGGCCCAAAATCACTAAAGGACAAATTTTTAGGTTGTAGTTGTAATTACATGAAGTTGTGGACTTTTGTTGGCCTAATAGTTGAGGTCATTGTTTTTTCGGCCCAAAATATGATGAGAGCAAAATGGTTTTGTCTCTTTAATGAGGATTGGATTTTCATTTCATTTAAAAAGGGTGATTATGAACCAAAGGCCTCGATAATTGAGCTATGTGATTGGCCGCTTTACATTATGAATTTCAAACGTGTTGCAAAAGGGCTTAAGTCCTTCAAATGTGTTGTAAAAGGGCATAAGCCCTTCTCTTTATTTCAAGTATTCTATTTTGTTTATTTGCATGCAAATTGGAATAGTTGGGTCCAACCAACAACTACCTACCTTAGACCCAAGGTCCAACACAAGGCTCTTGTTGGATCAAATCCAACCCCACACTACTCCATCAACAAAATGAATATCAAGTTGATAAGCGAGAGATGCTTTGAACATCTCTTCGTAGGCCTTCCATTGTGGTGGGCTCTAATCGTTTGTGACTCATACACCGCATTCGTCCAGTTCTGGGGTATACAAAGTATGTGCTTACATTCAGGAGAAGGTCAATATGATATTCTTCTGAGTGCAATGGTACCAATACGATATTTTTTTGAGGCATCATTCTTTAGAGGCCAAACCATATGGGTAACTAGAAAAGTTGTAAATGATTCTTCTGAGGTGGGCTTAGTAATGGTGAGTCTCCCATACCCCGTTAAGATTAAGACCCAATCAAGTAGTTTTAAAATCAACAATCTAATACGATTTTTGTTATGTTATGTAGTTAATGACATGCCTAAAATTACACCTACCATTATACTTGACACACCCCTGTGTGCTGGCCATCAAGTGAGGGAAATAAAAGTAACGAAAACAGAAGGAAAAAAAACGCAAAGTAGAAATTCAAGTATCCTAGAAAATCCTACATGGAGGGTGCTCTGTCAGAACTGCCTCGATGCCTCGTTCACCACCAACATCACCACTACCTCAAACTTGGAGGGGCGCAAAACAGAAAACGTGAGTGCGCAAAAACAAAGTTTTTTTTATTTTTATTTTTATTTATTTATTTATTTCAAACCATTTCAATATCAAGTAATAACCCCTCTCTATAAAACCCGTCATACTAAAAAAACGATAAAGCTCAAATACGCCATGCTAGAATATGCCATATCAAAATATCCAATCAAACAGTACAAGCATCAAGGAGGTGAAATAAAACAAATCATAATATCTATAGCTCCTCAACCACTATCCTGCACACGAGTTGGAACCACCTATATGCTACGATCCCACCCTAATATCCAACAATACGTGAAGGCTGTACGATGTATCGCAACTCACCTACGAGTCGGAACCACCTATAGTGGTCTGTACAACAGGCTAGTGAACGATCACGTGAAGGCTAGGCACTACTCTGGCGGAGCACAAACACCGAGGTGCAGGAATATGAGCTATAACTCAATCTCATCATCATCACAACTAGCATTAACATACTCACCTGACTTGCCTGAGCGTCTATGGTACCACAGCATAACCAACATAATCACAATAATAATATCAACAATATTCGACATGATATATATATTGAAAACAAAAACCCACTCACTGATACGTAGAAGGGTCGTAGCCCCCGAGGCTCGCGTGGCTGCGCTCGTCCTTAACATAGTCCTCACCTGTATACTTTGCTCAATTAGGGTGTTTGAATATACTAACATGCTTAACTCTATCCCACGAACACACCCCATATTTTTAGAAAAATTTTCTAAAGCCTCACGTACTTGCCAAGGCATGACCACACTCACCTCCCACGAGCAGGCACGTATTCCATCAAAACCAGAATATTCCGTTAACTTTAACCTGACGCCACTAGTCTCCGTTAGGAATATTCCGTCAACTTTGACGGAATATTCCGTCGTCTCTTACCTCAGGTCACCGGAATCCGACGCGGGCTGCTGCTACGTCGCCGAATTCTGGAAAATCTTCAAACCCATTTTTCTCACTCATTTCTTAACCAGACTTCACAAAATTTATGTCAAAATGAAGATTAGGACAAGGGAAAAAAGTTTATACCACTTTGAACCAGCCAACTTTAGACCTTGCTATCCCGACATCCGAAATCCTTTAAACTTGCTCTCCGAGCTTCGTGAGAAAGTTTTAAGACTCCCTGTGATCGTAAAAATGGATAAGACCTTCACGATCACGAGGTTTTCAAGGGTTCCAAGCCAAACCAAAGTTGGAACCTCGATCCATGCCAAAATTATGAGGTGTGATGTTGTCCATACTAATATAGTGAAAATGGAAGAAGTGTCAAGAAGGATGAGTGAGACAGAACCAGGAAAGGAAGAAAAGGGTGCAAATGTGCATGTGTGGATGTGTGTGTTGGGTCCAAATATGTGGGAAGAAACCCAATTGAACCAAAACATAGCCCAATCACACACACACGCAATCACTAACGATTTGGAACGGGCTATCACAATACTTGACAAAAAGGGCCTTAGGGGCCAACGTTTCCTTGCTTGGTATAATCACATTGAGAATGTCTCAAAGATGAATGACATATCATATGTGCTCAAGCAATCCCCTCCTCATGTACCTCCCACGAAACTAGCTTCAAAGGAGGAGTGTCAAAATTATACTAGACACTTTGAGGATGACTTACAAGCCAAATGCTTAATCCTCACTTCACTTAGTGAGGAGCTTAAGAAGCTTCAAGAGCACATGGACACTTCTTGTGCCATGGTTGATAGTTTGCATAAGATGCATGACATTAAGACAAGTAACATACGATTCTCAAATGTTTGTAGTCTATTGAATGCCAAAATGGCAAAGGGGACATCGGTCCAAAAACATGGACAAAAAATGGAAAGGATCGTTCAAGATCTTGAGAGTTTAGGAACTTCCATCGATGGGAAAAGTGCCCAAGACATTTTCCTTGCATCTCTCTCGGATGATTTCACTAAGTTGATAGTAAACTATAAAGTGAATAGATTCGATCATACTTTTTCCGAGATGATTGACATGTGCTGTGAGTTTGAACAAAGAAAAGGTTGCATAAGAAGAAGGCATGAAAATCCGAAGGAACATTCTTTCATTGTTGAAAGGATGAACGTTGGAAGAAGAAATATAAAGTGCTTCTGTCATAGAAAGTGCTTTTGGGGTCAAGCTATCTGGTCATGTAGGCATATGAATGCACAACAAGGGATCTCTAACCTTCCATGGTGGAAGGAGAATACTTTGATCGGGAGTCTTTGGCCAAAAAGTATCACATTGGATAGTAGACATGAAACAAACTATCACTTAAACAATGTGATTAAGAGTATTATATTAGAGAATGACTGTATTACTACTAGGTGTAACTCATGGTTTGTGGAAGCCCTGAAGATTATTTCAATTCATAATCGATCGTTAAGAGTAAAAATCGCCCACTGCCTCGCTAATTGGAACCTAATGGATAGTACATCGAGTAAGGATGAAATGAGAATGTTTCGGGTTTCGAGACCCAAAAGGGTTTTTGGTCCACAAGGCCCATTGAGTTTAGGTTGTGTGACAACTAAAACTAAATGGAAATTAACCCAAAAACAAAGTCAAAACCGGCCAAGGGTGAGTGGTAGCAAACTTGGGCAACTTTATAGCCATTCTCTCACTAGGGTTTTGTTGAGGCAATTAAGAGAAACTTTTTCTCCCTTGTTCTCTATAGAGGCCAGCCACTTAGGGAGACTTTTACTAGCATCTAAAATCTCTATAAGTCATTCATCTTCTCTTCACACTACATCTTTGGTGTGGAGACTTGGAGGTACCAAACCTTTGGTATTTTTGGAGTTCCTTTGCTCACATCCTCAAGGAGCAAAGGAGATTCAAAAGGAAGAAAAATCACTAAGAAGATTCAAGAACTCGAAAACTCTTTTCTCTTTGATTTTGTTAAAGAGTCTTATTGGTTCACCATTCACTAGTAAAGTGTTAATACACAAAAATTTTCCTTCACATATGCAAAGTGGGTTTGAAAGAATTATTGTCCATGCTGATAATTCAATTGATATGGCTAATAGGCAAGCTGGTATGAAAACTCAAAAACACCATATGCTAGTTGTTGAGCCATGCAGAATATCTTGTAAATTAACTTTGCATAGTAGACTAACAAAGAACGCAAGAAGTTTTTGGCCGGAAAAGCTCACCTCTGGGTTGAAAAACCGGGGACTCTCCATTGAGTCCAATCCACTATCTACGGAGCAGCTTTATTTTTGTGTAACCCTTGCCTTAAACGAGACAGATTCCTTCAGTTTTCATAAAATGGCAGCTCTTCATAAGCTCTTCACCTTAGCTCCTCCTGAACTCTTCACCTTATGGCACCAAGACCAACACACAAACTATTCATATAATGATATGAAAATAATATGCATATCTGGATTCAAATGTCGAGTCAATTTCACTATCAAATGTCAAGTCAGTTTTGCCAGACAAAATCACAATGACAATGCTACCCTAATATGCAACGCTTTGGGTGTTTACTGCATGAGTGTGATTTTGTGGCTAGGGTTTCTAATGAGGAACTCAAAGAGGCAGCTCAAAGTTAAGCAAGCAGTTTCAAAGAGAGCATATAAAACAAATTTAAGAAAGCTTAATGCTCCTAGGGGATCTGAGATAGAGAAGGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

1773

Amino Acids

205.13

Weight (kDa)

9.69

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 1091 - 1226 2.8e-10 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 211
Acc16I TGCGCA 1 cut(s) 1689
Acc65I GGTACC 3 cut(s) 1258, 2184, 4457
AccB1I GGYRCC 4 cut(s) 1258, 2184, 4457, 5007
AccI GTMKAC 4 cut(s) 1198, 2341, 3940, 4792
AccII CGCG 3 cut(s) 213, 2304, 2609
AciI CCGC 5 cut(s) 267, 860, 1176, 2082, 2609
AclI AACGTT 2 cut(s) 3169, 3771
AclWI GGATC 6 cut(s) 1057, 1944, 2902, 3539, 3872, 5302
AcoI YGGCCR 7 cut(s) 61, 316, 857, 1504, 3914, 4256, 4820
AcuI CTGAAG 2 cut(s) 4061, 4921
AcvI CACGTG 1 cut(s) 2059
AcyI GRCGYC 3 cut(s) 180, 208, 2525
AdeI CACNNNGTG 1 cut(s) 3353
AfiI CCNNNNNNNGG 6 cut(s) 1036, 1192, 2476, 2634, 3570, 4261
AflII CTTAAG 2 cut(s) 899, 3362
AflIII ACRYGT 2 cut(s) 883, 3681
AhdI GACNNNNNGTC 1 cut(s) 1025
AhlI ACTAGT 2 cut(s) 2530, 4610
AjnI CCWGG 1 cut(s) 2995
AjuI GAANNNNNNNTTGG 2 cut(s) 3799, 3831
AleI CACNNNNGTG 2 cut(s) 1495, 2098
Alw21I GWGCWC 4 cut(s) 1602, 2089, 3234, 3381
AlwI GGATC 6 cut(s) 1057, 1944, 2902, 3539, 3872, 5302
Ama87I CYCGRG 1 cut(s) 2294
ApeKI GCWGC 7 cut(s) 222, 2309, 2613, 2616, 4891, 4955, 5221
ArsI GACNNNNNNTTYG 8 cut(s) 2679, 2711, 4231, 4263, 5064, 5088, 5096, 5120
Asp700I GAANNNNTTC 2 cut(s) 2642, 3650
Asp718I GGTACC 3 cut(s) 1258, 2184, 4457
AspA2I CCTAGG 1 cut(s) 5288
AspLEI GCGC 3 cut(s) 1668, 1690, 2314
AsuC2I CCSGG 1 cut(s) 4853
AvaI CYCGRG 1 cut(s) 2294
AvaII GGWCC 5 cut(s) 994, 1027, 3047, 3502, 4178
AvrII CCTAGG 1 cut(s) 5288
AxyI CCTNAGG 2 cut(s) 2588, 3158
BaeGI GKGCMC 1 cut(s) 3583
BalI TGGCCA 2 cut(s) 1506, 3916
BanI GGYRCC 4 cut(s) 1258, 2184, 4457, 5007
BanII GRGCYC 1 cut(s) 1153
BauI CACGAG 3 cut(s) 1923, 2471, 2863
BbrPI CACGTG 1 cut(s) 2059
Bbv12I GWGCWC 4 cut(s) 1602, 2089, 3234, 3381
BbvI GCAGC 7 cut(s) 234, 2296, 2600, 2603, 4903, 4967, 5233
BccI CCATC 7 cut(s) 86, 586, 1082, 1515, 2497, 3563, 3572
BceAI ACGGC 1 cut(s) 76
BcgI CGANNNNNNTGC 2 cut(s) 3588, 3622
BciT130I CCWGG 1 cut(s) 2997
BciVI GTATCC 2 cut(s) 17, 1583
BcnI CCSGG 1 cut(s) 4853
BcuI ACTAGT 2 cut(s) 2530, 4610
BfmI CTRYAG 3 cut(s) 1896, 2023, 4356
BfrI CTTAAG 2 cut(s) 899, 3362
BfuI GTATCC 2 cut(s) 17, 1583
BglI GCCNNNNNGGC 3 cut(s) 2297, 3162, 3485
BglII AGATCT 1 cut(s) 3541
BisI GCNGC 8 cut(s) 223, 860, 2310, 2614, 2617, 4892, 4956, 5222
BlnI CCTAGG 1 cut(s) 5288
BlsI GCNGC 8 cut(s) 224, 861, 2311, 2615, 2618, 4893, 4957, 5223
Bme1390I CCNGG 2 cut(s) 2997, 4853
Bme18I GGWCC 5 cut(s) 994, 1027, 3047, 3502, 4178
BmeRI GACNNNNNGTC 1 cut(s) 1025
BmeT110I CYCGRG 1 cut(s) 2294
BmrFI CCNGG 2 cut(s) 2997, 4853
BmsI GCATC 9 cut(s) 234, 278, 1100, 1292, 1609, 1870, 3411, 3608, 4400
BoxI GACNNNNGTC 1 cut(s) 3500
BplI GAGNNNNNCTC 2 cut(s) 3188, 3220
Bpu10I CCTNAGC 2 cut(s) 2172, 4978
BpuEI CTTGAG 3 cut(s) 3218, 3566, 4485
BpuMI CCSGG 1 cut(s) 4853
Bsa29I ATCGAT 2 cut(s) 3567, 4063
BsaAI YACGTR 6 cut(s) 638, 1975, 2059, 2276, 2438, 2483
BsaHI GRCGYC 3 cut(s) 180, 208, 2525
BsaI GGTCTC 1 cut(s) 4153
BsaWI WCCGGW 1 cut(s) 2596
BsaXI ACNNNNNCTCC 6 cut(s) 2780, 2810, 4327, 4357, 4966, 4996
Bsc4I CCNNNNNNNGG 6 cut(s) 1036, 1192, 2476, 2634, 3570, 4261
Bse118I RCCGGY 2 cut(s) 127, 4254
Bse1I ACTGG 1 cut(s) 1186
Bse21I CCTNAGG 2 cut(s) 2588, 3158
Bse3DI GCAATG 1 cut(s) 1260
BseBI CCWGG 1 cut(s) 2997
BseCI ATCGAT 2 cut(s) 3567, 4063
BseGI GGATG 9 cut(s) 578, 2772, 2985, 3039, 3319, 3616, 3771, 4138, 4495
BseLI CCNNNNNNNGG 6 cut(s) 1036, 1192, 2476, 2634, 3570, 4261
BseMI GCAATG 1 cut(s) 1260
BseMII CTCAG 5 cut(s) 1238, 1332, 2163, 2602, 5289
BseNI ACTGG 1 cut(s) 1186
BseRI GAGGAG 5 cut(s) 1893, 3237, 3296, 3371, 4974
BseSI GKGCMC 1 cut(s) 3583
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 7 cut(s) 234, 2296, 2600, 2603, 4903, 4967, 5233
BsgI GTGCAG 2 cut(s) 1903, 2122
Bsh1236I CGCG 3 cut(s) 213, 2304, 2609
Bsh1285I CGRYCG 2 cut(s) 64, 4067
BshNI GGYRCC 4 cut(s) 1258, 2184, 4457, 5007
BshVI ATCGAT 2 cut(s) 3567, 4063
BsiEI CGRYCG 2 cut(s) 64, 4067
BsiHKAI GWGCWC 4 cut(s) 1602, 2089, 3234, 3381
BsiHKCI CYCGRG 1 cut(s) 2294
BsiSI CCGG 5 cut(s) 128, 2597, 4255, 4823, 4852
BslFI GGGAC 3 cut(s) 354, 3508, 4869
BslI CCNNNNNNNGG 6 cut(s) 1036, 1192, 2476, 2634, 3570, 4261
BsmBI CGTCTC 2 cut(s) 199, 2584
BsmFI GGGAC 3 cut(s) 354, 3508, 4869
BsmI GAATGC 3 cut(s) 1178, 3481, 3856
Bso31I GGTCTC 1 cut(s) 4153
BsoBI CYCGRG 1 cut(s) 2294
Bsp1286I GDGCHC 6 cut(s) 1153, 1602, 2089, 3234, 3381, 3583
Bsp1407I TGTACA 1 cut(s) 2034
Bsp19I CCATGG 2 cut(s) 3399, 3878
BspACI CCGC 5 cut(s) 267, 860, 1176, 2082, 2609
BspCNI CTCAG 5 cut(s) 1239, 1333, 2164, 2601, 5290
BspDI ATCGAT 2 cut(s) 3567, 4063
BspFNI CGCG 3 cut(s) 213, 2304, 2609
BspPI GGATC 6 cut(s) 1057, 1944, 2902, 3539, 3872, 5302
BspQI GCTCTTC 2 cut(s) 4965, 4976
BspT107I GGYRCC 4 cut(s) 1258, 2184, 4457, 5007
BspTI CTTAAG 2 cut(s) 899, 3362
BspTNI GGTCTC 1 cut(s) 4153
BsrDI GCAATG 1 cut(s) 1260
BsrFI RCCGGY 2 cut(s) 127, 4254
BsrGI TGTACA 1 cut(s) 2034
BsrI ACTGG 1 cut(s) 1186
BssAI RCCGGY 2 cut(s) 127, 4254
BssNAI GTATAC 2 cut(s) 1199, 2342
BssNI GRCGYC 3 cut(s) 180, 208, 2525
BssSI CACGAG 3 cut(s) 1923, 2471, 2863
BssT1I CCWWGG 7 cut(s) 243, 1023, 2446, 3399, 3878, 4259, 5288
Bst1107I GTATAC 2 cut(s) 1199, 2342
Bst2BI CACGAG 3 cut(s) 1923, 2471, 2863
Bst2UI CCWGG 1 cut(s) 2997
Bst4CI ACNGT 4 cut(s) 166, 308, 1854, 4001
Bst6I CTCTTC 5 cut(s) 1130, 4428, 4965, 4976, 5000
BstACI GRCGYC 3 cut(s) 180, 208, 2525
BstAFI CTTAAG 2 cut(s) 899, 3362
BstAPI GCANNNNNTGC 1 cut(s) 3421
BstAUI TGTACA 1 cut(s) 2034
BstBAI YACGTR 6 cut(s) 638, 1975, 2059, 2276, 2438, 2483
BstC8I GCNNGC 9 cut(s) 262, 975, 1504, 2302, 2478, 2611, 4366, 4713, 5238
BstDSI CCRYGG 2 cut(s) 3399, 3878
BstEII GGTNACC 1 cut(s) 2592
BstF5I GGATG 9 cut(s) 578, 2772, 2985, 3039, 3319, 3616, 3771, 4138, 4495
BstFNI CGCG 3 cut(s) 213, 2304, 2609
BstHHI GCGC 3 cut(s) 1668, 1690, 2314
BstMCI CGRYCG 2 cut(s) 64, 4067
BstNI CCWGG 1 cut(s) 2997
BstNSI RCATGY 6 cut(s) 140, 977, 1459, 2380, 3029, 3685
BstPAI GACNNNNGTC 1 cut(s) 3500
BstPI GGTNACC 1 cut(s) 2592
BstSCI CCNGG 2 cut(s) 2995, 4851
BstSFI CTRYAG 3 cut(s) 1896, 2023, 4356
BstSLI GKGCMC 1 cut(s) 3583
BstSNI TACGTA 2 cut(s) 638, 2276
BstUI CGCG 3 cut(s) 213, 2304, 2609
BstV1I GCAGC 7 cut(s) 234, 2296, 2600, 2603, 4903, 4967, 5233
BstX2I RGATCY 3 cut(s) 3541, 3864, 5294
BstXI CCANNNNNNTGG 4 cut(s) 1146, 3057, 3512, 4468
BstYI RGATCY 3 cut(s) 3541, 3864, 5294
BstZ17I GTATAC 2 cut(s) 1199, 2342
BstZI CGGCCG 1 cut(s) 61
Bsu15I ATCGAT 2 cut(s) 3567, 4063
Bsu36I CCTNAGG 2 cut(s) 2588, 3158
BsuI GTATCC 2 cut(s) 17, 1583
BsuTUI ATCGAT 2 cut(s) 3567, 4063
BtgI CCRYGG 2 cut(s) 3399, 3878
BtsCI GGATG 9 cut(s) 578, 2772, 2985, 3039, 3319, 3616, 3771, 4138, 4495
BtsI GCAGTG 1 cut(s) 4087
BtsIMutI CAGTG 3 cut(s) 313, 2267, 4087
Cac8I GCNNGC 9 cut(s) 262, 975, 1504, 2302, 2478, 2611, 4366, 4713, 5238
CfoI GCGC 3 cut(s) 1668, 1690, 2314
Cfr10I RCCGGY 2 cut(s) 127, 4254
ClaI ATCGAT 2 cut(s) 3567, 4063
CseI GACGC 5 cut(s) 188, 291, 2165, 2533, 2615
DraI TTTAAA 4 cut(s) 526, 808, 1408, 2787
DraIII CACNNNGTG 1 cut(s) 3353
DriI GACNNNNNGTC 1 cut(s) 1025
EaeI YGGCCR 7 cut(s) 61, 316, 857, 1504, 3914, 4256, 4820
EagI CGGCCG 1 cut(s) 61
Eam1104I CTCTTC 5 cut(s) 1130, 4428, 4965, 4976, 5000
Eam1105I GACNNNNNGTC 1 cut(s) 1025
EarI CTCTTC 5 cut(s) 1130, 4428, 4965, 4976, 5000
EciI GGCGGA 1 cut(s) 2097
EclXI CGGCCG 1 cut(s) 61
Eco105I TACGTA 2 cut(s) 638, 2276
Eco130I CCWWGG 7 cut(s) 243, 1023, 2446, 3399, 3878, 4259, 5288
Eco147I AGGCCT 2 cut(s) 833, 1134
Eco24I GRGCYC 1 cut(s) 1153
Eco31I GGTCTC 1 cut(s) 4153
Eco47I GGWCC 5 cut(s) 994, 1027, 3047, 3502, 4178
Eco52I CGGCCG 1 cut(s) 61
Eco57I CTGAAG 2 cut(s) 4061, 4921
Eco72I CACGTG 1 cut(s) 2059
Eco81I CCTNAGG 2 cut(s) 2588, 3158
Eco88I CYCGRG 1 cut(s) 2294
Eco91I GGTNACC 1 cut(s) 2592
EcoO109I RGGNCCY 1 cut(s) 3154
EcoO65I GGTNACC 1 cut(s) 2592
EcoRI GAATTC 1 cut(s) 2630
EcoRII CCWGG 1 cut(s) 2995
EcoT14I CCWWGG 7 cut(s) 243, 1023, 2446, 3399, 3878, 4259, 5288
EcoT22I ATGCAT 2 cut(s) 3426, 5060
EcoT38I GRGCYC 1 cut(s) 1153
ErhI CCWWGG 7 cut(s) 243, 1023, 2446, 3399, 3878, 4259, 5288
Esp3I CGTCTC 2 cut(s) 199, 2584
FalI AAGNNNNNCTT 2 cut(s) 4321, 4353
FaqI GGGAC 3 cut(s) 354, 3508, 4869
FauI CCCGC 2 cut(s) 274, 2602
FauNDI CATATG 5 cut(s) 1307, 3226, 3846, 4647, 4740
FblI GTMKAC 4 cut(s) 1198, 2341, 3940, 4792
Fnu4HI GCNGC 8 cut(s) 223, 860, 2310, 2614, 2617, 4892, 4956, 5222
FokI GGATG 9 cut(s) 565, 2759, 2992, 3046, 3326, 3623, 3778, 4145, 4482
FriOI GRGCYC 1 cut(s) 1153
Fsp4HI GCNGC 8 cut(s) 223, 860, 2310, 2614, 2617, 4892, 4956, 5222
FspI TGCGCA 1 cut(s) 1689
GlaI GCGC 3 cut(s) 1667, 1689, 2313
GluI GCNGC 8 cut(s) 223, 860, 2310, 2614, 2617, 4892, 4956, 5222
HapII CCGG 5 cut(s) 128, 2597, 4255, 4823, 4852
HgaI GACGC 5 cut(s) 188, 291, 2165, 2533, 2615
HhaI GCGC 3 cut(s) 1668, 1690, 2314
Hin1I GRCGYC 3 cut(s) 180, 208, 2525
Hin6I GCGC 3 cut(s) 1666, 1688, 2312
HinP1I GCGC 3 cut(s) 1666, 1688, 2312
HincII GTYRAC 3 cut(s) 304, 2512, 2556
HindII GTYRAC 3 cut(s) 304, 2512, 2556
HindIII AAGCTT 2 cut(s) 3368, 5276
HpaI GTTAAC 1 cut(s) 2512
HpaII CCGG 5 cut(s) 128, 2597, 4255, 4823, 4852
Hpy99I CGWCG 5 cut(s) 182, 213, 2579, 2609, 2627
HpyCH4III ACNGT 4 cut(s) 166, 308, 1854, 4001
Hsp92I GRCGYC 3 cut(s) 180, 208, 2525
HspAI GCGC 3 cut(s) 1666, 1688, 2312
KpnI GGTACC 3 cut(s) 1262, 2188, 4461
KspAI GTTAAC 1 cut(s) 2512
LguI GCTCTTC 2 cut(s) 4965, 4976
LmnI GCTCC 8 cut(s) 124, 1906, 2084, 3358, 4505, 4888, 4987, 5290
Lsp1109I GCAGC 7 cut(s) 234, 2296, 2600, 2603, 4903, 4967, 5233
LweI GCATC 9 cut(s) 234, 278, 1100, 1292, 1609, 1870, 3411, 3608, 4400
MaeIII GTNAC 9 cut(s) 166, 1163, 1312, 1528, 2592, 3440, 4016, 4207, 4906
MfeI CAATTG 2 cut(s) 3071, 4692
MflI RGATCY 3 cut(s) 3541, 3864, 5294
MhlI GDGCHC 6 cut(s) 1153, 1602, 2089, 3234, 3381, 3583
MlsI TGGCCA 2 cut(s) 1506, 3916
MluNI TGGCCA 2 cut(s) 1506, 3916
MlyI GAGTC 9 cut(s) 1160, 1372, 2019, 2817, 3916, 4597, 4851, 4877, 5088
Mox20I TGGCCA 2 cut(s) 1506, 3916
Mph1103I ATGCAT 2 cut(s) 3426, 5060
MroXI GAANNNNTTC 2 cut(s) 2642, 3650
MscI TGGCCA 2 cut(s) 1506, 3916
MslI CAYNNNNRTG 6 cut(s) 1144, 1495, 1940, 2098, 3849, 5177
Msp20I TGGCCA 2 cut(s) 1506, 3916
MspCI CTTAAG 2 cut(s) 899, 3362
MspI CCGG 5 cut(s) 128, 2597, 4255, 4823, 4852
MspR9I CCNGG 2 cut(s) 2997, 4853
MunI CAATTG 2 cut(s) 3071, 4692
Mva1269I GAATGC 3 cut(s) 1178, 3481, 3856
MvaI CCWGG 1 cut(s) 2997
MvnI CGCG 3 cut(s) 213, 2304, 2609
NciI CCSGG 1 cut(s) 4853
NcoI CCATGG 2 cut(s) 3399, 3878
NdeI CATATG 5 cut(s) 1307, 3226, 3846, 4647, 4740
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 4 cut(s) 166, 1163, 2592, 4207
NsbI TGCGCA 1 cut(s) 1689
NsiI ATGCAT 2 cut(s) 3426, 5060
NspI RCATGY 6 cut(s) 140, 977, 1459, 2380, 3029, 3685
OliI CACNNNNGTG 2 cut(s) 1495, 2098
PaeI GCATGC 1 cut(s) 977
PceI AGGCCT 2 cut(s) 833, 1134
PciI ACATGT 1 cut(s) 3681
PciSI GCTCTTC 2 cut(s) 4965, 4976
PcsI WCGNNNNNNNCGW 1 cut(s) 2292
PctI GAATGC 3 cut(s) 1178, 3481, 3856
PdmI GAANNNNTTC 2 cut(s) 2642, 3650
PkrI GCNGC 8 cut(s) 224, 861, 2311, 2615, 2618, 4893, 4957, 5223
Ple19I CGATCG 1 cut(s) 4067
PleI GAGTC 9 cut(s) 1160, 1371, 2018, 2817, 3915, 4596, 4851, 4876, 5087
PmaCI CACGTG 1 cut(s) 2059
PmlI CACGTG 1 cut(s) 2059
PpsI GAGTC 9 cut(s) 1160, 1371, 2018, 2817, 3915, 4596, 4851, 4876, 5087
Ppu21I YACGTR 6 cut(s) 638, 1975, 2059, 2276, 2438, 2483
PscI ACATGT 1 cut(s) 3681
PshAI GACNNNNGTC 1 cut(s) 3500
Psp1406I AACGTT 2 cut(s) 3169, 3771
Psp6I CCWGG 1 cut(s) 2995
PspCI CACGTG 1 cut(s) 2059
PspEI GGTNACC 1 cut(s) 2592
PspGI CCWGG 1 cut(s) 2995
PsrI GAACNNNNNNTAC 2 cut(s) 58, 90
PsuI RGATCY 3 cut(s) 3541, 3864, 5294
PvuI CGATCG 1 cut(s) 4067
RseI CAYNNNNRTG 6 cut(s) 1144, 1495, 1940, 2098, 3849, 5177
SapI GCTCTTC 2 cut(s) 4965, 4976
SatI GCNGC 8 cut(s) 223, 860, 2310, 2614, 2617, 4892, 4956, 5222
SchI GAGTC 9 cut(s) 1160, 1372, 2019, 2817, 3916, 4597, 4851, 4877, 5088
ScrFI CCNGG 2 cut(s) 2997, 4853
SduI GDGCHC 6 cut(s) 1153, 1602, 2089, 3234, 3381, 3583
SfaNI GCATC 9 cut(s) 234, 278, 1100, 1292, 1609, 1870, 3411, 3608, 4400
SfcI CTRYAG 3 cut(s) 1896, 2023, 4356
SfiI GGCCNNNNNGGCC 1 cut(s) 3162
SinI GGWCC 5 cut(s) 994, 1027, 3047, 3502, 4178
SmiMI CAYNNNNRTG 6 cut(s) 1144, 1495, 1940, 2098, 3849, 5177
SmlI CTYRAG 5 cut(s) 899, 3233, 3362, 3545, 4500
SmoI CTYRAG 5 cut(s) 899, 3233, 3362, 3545, 4500
SnaBI TACGTA 2 cut(s) 638, 2276
SpeI ACTAGT 2 cut(s) 2530, 4610
SphI GCATGC 1 cut(s) 977
SseBI AGGCCT 2 cut(s) 833, 1134
SsiI CCGC 5 cut(s) 267, 860, 1176, 2082, 2609
SspI AATATT 4 cut(s) 2229, 2504, 2548, 2570
StuI AGGCCT 2 cut(s) 833, 1134
StyD4I CCNGG 2 cut(s) 2995, 4851
StyI CCWWGG 7 cut(s) 243, 1023, 2446, 3399, 3878, 4259, 5288
TaaI ACNGT 4 cut(s) 166, 308, 1854, 4001
TaqII GACCGA 2 cut(s) 185, 3490
TatI WGTACW 3 cut(s) 1854, 2034, 4118
TauI GCSGC 1 cut(s) 862
TscAI CASTG 3 cut(s) 313, 2274, 4094
TseFI GTSAC 4 cut(s) 166, 1163, 2592, 4207
TseI GCWGC 7 cut(s) 222, 2309, 2613, 2616, 4891, 4955, 5221
Tsp45I GTSAC 4 cut(s) 166, 1163, 2592, 4207
TspGWI ACGGA 7 cut(s) 102, 2497, 2527, 2541, 2563, 2579, 4901
TspRI CASTG 3 cut(s) 313, 2274, 4094
Vha464I CTTAAG 2 cut(s) 899, 3362
VpaK11BI GGWCC 5 cut(s) 994, 1027, 3047, 3502, 4178
XceI RCATGY 6 cut(s) 140, 977, 1459, 2380, 3029, 3685
XcmI CCANNNNNNNNNTGG 1 cut(s) 2894
XmaJI CCTAGG 1 cut(s) 5288
XmiI GTMKAC 4 cut(s) 1198, 2341, 3940, 4792
XmnI GAANNNNTTC 2 cut(s) 2642, 3650
ZraI GACGTC 1 cut(s) 209
Zsp2I ATGCAT 2 cut(s) 3426, 5060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.