pycom854g00010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000854
OG0008025
Physical Location & Seq
Forward (+)
6782 .. 7576
795 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGGCTAAGAAAAACGGAGCTTTGCATTTGAATGCGTTGCTGTATCATTTATCGGAGGAGTTTGGTGAAGAGCCGTTGCCACCAGAGTGGTATCAGAAGGCATTCCCCAAGTTGACGAAATTAACCCAATTGTTGAAAAATGTCGATTTGATTGATGGAAGGCTTGTTAATATCAGTGATGGTTCGATAATCATTGACGACCGTGTTGAACAAAGAATGCTTACTTTCAAGTCACCTGCCACGGTATTTATTGGAGCTCCATTGCTACAGAAAGGGCTGAAGAATAATGTGGCGGCATTTCGGGAGGTGGCACAACACCGTATATGGACTGGCGCGCTTACGGAGGTACTCAATGGATTGAATTCCGAGCTGGACTGTTTGGATTATCGGTGCCCAAGTAAACGGACGAAGATGGGACAGCAGATAGTTTGTAGCTGTCCAAAGTTCTTGGCTGACACATCCACATCTGTTGCATATGAGCACGACTCCTCTTCATGGACACGCCTTGCGCCTGCAAAAGTTATTGACTCTTCCGGTTTGCAGAAATGGGAAGAGGTTCTTGATATATTCAACGACCTCATTGTTTGGTTGAAAAACGAAAGGGAATTGTCTTCTATCCGCTGTAAACGCAGTCTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

24.09

Weight (kDa)

7.63

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 244
Acc36I ACCTGC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 390
AccII CGCG 1 cut(s) 335
AciI CCGC 2 cut(s) 293, 619
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 1 cut(s) 299
AfaI GTAC 1 cut(s) 348
AfiI CCNNNNNNNGG 1 cut(s) 495
AgsI TTSAA 7 cut(s) 31, 136, 209, 229, 361, 571, 592
AleI CACNNNNGTG 1 cut(s) 85
AluBI AGCT 4 cut(s) 20, 257, 370, 435
AluI AGCT 4 cut(s) 20, 257, 370, 435
Alw21I GWGCWC 2 cut(s) 259, 483
Alw26I GTCTC 1 cut(s) 638
ApoI RAATTY 1 cut(s) 361
Asp700I GAANNNNTTC 2 cut(s) 101, 555
AspLEI GCGC 3 cut(s) 335, 337, 511
AsuHPI GGTGA 2 cut(s) 77, 225
BaeGI GKGCMC 1 cut(s) 395
BanI GGYRCC 1 cut(s) 390
BanII GRGCYC 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 603
Bbv12I GWGCWC 2 cut(s) 259, 483
BccI CCATC 3 cut(s) 149, 173, 406
BceAI ACGGC 1 cut(s) 58
BcoDI GTCTC 1 cut(s) 638
BfmI CTRYAG 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 244
BisI GCNGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 295
BmiI GGNNCC 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 603
BplI GAGNNNNNCTC 2 cut(s) 470, 502
BsaJI CCNNGG 1 cut(s) 240
BsaWI WCCGGW 1 cut(s) 533
BsaXI ACNNNNNCTCC 1 cut(s) 618
Bsc4I CCNNNNNNNGG 1 cut(s) 495
Bse1I ACTGG 1 cut(s) 334
Bse3DI GCAATG 1 cut(s) 260
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 458
BseLI CCNNNNNNNGG 1 cut(s) 495
BseMI GCAATG 1 cut(s) 260
BseNI ACTGG 1 cut(s) 334
BsePI GCGCGC 1 cut(s) 333
BseRI GAGGAG 2 cut(s) 71, 478
BseSI GKGCMC 1 cut(s) 395
Bsh1236I CGCG 1 cut(s) 335
Bsh1285I CGRYCG 1 cut(s) 202
BshNI GGYRCC 1 cut(s) 390
BsiEI CGRYCG 1 cut(s) 202
BsiHKAI GWGCWC 2 cut(s) 259, 483
BsiSI CCGG 1 cut(s) 534
BslFI GGGAC 1 cut(s) 429
BslI CCNNNNNNNGG 1 cut(s) 495
BsmAI GTCTC 1 cut(s) 638
BsmFI GGGAC 1 cut(s) 429
BsmI GAATGC 3 cut(s) 37, 101, 222
Bsp1286I GDGCHC 3 cut(s) 259, 395, 483
BspACI CCGC 2 cut(s) 293, 619
BspFNI CGCG 1 cut(s) 335
BspLI GGNNCC 1 cut(s) 392
BspMI ACCTGC 1 cut(s) 244
BspQI GCTCTTC 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 390
BsrDI GCAATG 1 cut(s) 260
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 240
BssHII GCGCGC 1 cut(s) 333
Bst4CI ACNGT 4 cut(s) 203, 244, 320, 377
Bst6I CTCTTC 4 cut(s) 63, 496, 535, 546
BstC8I GCNNGC 2 cut(s) 335, 513
BstDEI CTNAG 1 cut(s) 6
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 1 cut(s) 458
BstFNI CGCG 1 cut(s) 335
BstHHI GCGC 3 cut(s) 335, 337, 511
BstMAI GTCTC 1 cut(s) 638
BstMCI CGRYCG 1 cut(s) 202
BstMWI GCNNNNNNNGC 1 cut(s) 627
BstSFI CTRYAG 1 cut(s) 266
BstSLI GKGCMC 1 cut(s) 395
BstUI CGCG 1 cut(s) 335
BstV2I GAAGAC 1 cut(s) 603
BstXI CCANNNNNNTGG 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 240
BtsCI GGATG 1 cut(s) 458
BtsIMutI CAGTG 1 cut(s) 181
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 2 cut(s) 335, 513
CfoI GCGC 3 cut(s) 335, 337, 511
Csp6I GTAC 1 cut(s) 347
CspCI CAANNNNNGTGG 2 cut(s) 451, 486
CviAII CATG 1 cut(s) 495
CviJI RGCY 9 cut(s) 5, 20, 73, 163, 257, 277, 370, 435, 452
CviKI_1 RGCY 9 cut(s) 5, 20, 73, 163, 257, 277, 370, 435, 452
CviQI GTAC 1 cut(s) 347
DdeI CTNAG 1 cut(s) 6
Eam1104I CTCTTC 4 cut(s) 63, 496, 535, 546
EarI CTCTTC 4 cut(s) 63, 496, 535, 546
Ecl136II GAGCTC 1 cut(s) 257
Eco24I GRGCYC 1 cut(s) 259
Eco53kI GAGCTC 1 cut(s) 257
Eco57I CTGAAG 1 cut(s) 299
EcoICRI GAGCTC 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 361
EcoT38I GRGCYC 1 cut(s) 259
FaeI CATG 1 cut(s) 498
FaiI YATR 6 cut(s) 323, 325, 475, 477, 496, 566
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FaqI GGGAC 1 cut(s) 429
FatI CATG 1 cut(s) 494
FauNDI CATATG 1 cut(s) 475
Fnu4HI GCNGC 1 cut(s) 294
FokI GGATG 1 cut(s) 445
FriOI GRGCYC 1 cut(s) 259
Fsp4HI GCNGC 1 cut(s) 294
GlaI GCGC 3 cut(s) 334, 336, 510
GluI GCNGC 1 cut(s) 294
HapII CCGG 1 cut(s) 534
HhaI GCGC 3 cut(s) 335, 337, 511
Hin1II CATG 1 cut(s) 498
Hin6I GCGC 3 cut(s) 333, 335, 509
HinP1I GCGC 3 cut(s) 333, 335, 509
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HinfI GANTC 2 cut(s) 485, 527
HpaII CCGG 1 cut(s) 534
HphI GGTGA 2 cut(s) 77, 225
Hpy166II GTNNAC 3 cut(s) 114, 401, 626
Hpy188I TCNGA 3 cut(s) 55, 96, 367
Hpy188III TCNNGA 2 cut(s) 302, 560
Hpy8I GTNNAC 3 cut(s) 114, 401, 626
HpyAV CCTTC 2 cut(s) 91, 153
HpyCH4III ACNGT 4 cut(s) 203, 244, 320, 377
HpyCH4V TGCA 4 cut(s) 25, 473, 515, 541
HpyF10VI GCNNNNNNNGC 1 cut(s) 627
HpyF3I CTNAG 1 cut(s) 6
Hsp92II CATG 1 cut(s) 498
HspAI GCGC 3 cut(s) 333, 335, 509
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 3 cut(s) 17, 254, 262
LpnPI CCDG 6 cut(s) 96, 249, 315, 356, 525, 547
MaeIII GTNAC 1 cut(s) 231
MboII GAAGA 7 cut(s) 80, 292, 421, 483, 522, 563, 603
MfeI CAATTG 1 cut(s) 128
MhlI GDGCHC 3 cut(s) 259, 395, 483
MluCI AATT 4 cut(s) 119, 128, 361, 605
MlyI GAGTC 2 cut(s) 479, 521
MnlI CCTC 6 cut(s) 49, 298, 337, 499, 547, 587
MroXI GAANNNNTTC 2 cut(s) 101, 555
MseI TTAA 3 cut(s) 122, 168, 640
MslI CAYNNNNRTG 2 cut(s) 30, 85
MspA1I CMGCKG 1 cut(s) 621
MspI CCGG 1 cut(s) 534
MunI CAATTG 1 cut(s) 128
Mva1269I GAATGC 3 cut(s) 37, 101, 222
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 1 cut(s) 627
NdeI CATATG 1 cut(s) 475
NlaIII CATG 1 cut(s) 498
NlaIV GGNNCC 1 cut(s) 392
NmuCI GTSAC 1 cut(s) 231
OliI CACNNNNGTG 1 cut(s) 85
PaqCI CACCTGC 1 cut(s) 244
PauI GCGCGC 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 63
PctI GAATGC 3 cut(s) 37, 101, 222
PdmI GAANNNNTTC 2 cut(s) 101, 555
PkrI GCNGC 1 cut(s) 295
PleI GAGTC 2 cut(s) 479, 521
PpsI GAGTC 2 cut(s) 479, 521
Psp124BI GAGCTC 1 cut(s) 259
PspN4I GGNNCC 1 cut(s) 392
PteI GCGCGC 1 cut(s) 333
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
RseI CAYNNNNRTG 2 cut(s) 30, 85
SacI GAGCTC 1 cut(s) 259
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 3 cut(s) 122, 168, 640
SatI GCNGC 1 cut(s) 294
SchI GAGTC 2 cut(s) 479, 521
SduI GDGCHC 3 cut(s) 259, 395, 483
SetI ASST 9 cut(s) 22, 238, 259, 309, 348, 372, 437, 558, 579
SfcI CTRYAG 1 cut(s) 266
SmiMI CAYNNNNRTG 2 cut(s) 30, 85
Sse9I AATT 4 cut(s) 119, 128, 361, 605
SsiI CCGC 2 cut(s) 293, 619
SstI GAGCTC 1 cut(s) 259
TaaI ACNGT 4 cut(s) 203, 244, 320, 377
TaqI TCGA 2 cut(s) 144, 185
TasI AATT 4 cut(s) 119, 128, 361, 605
TauI GCSGC 1 cut(s) 296
Tru1I TTAA 3 cut(s) 122, 168, 640
Tru9I TTAA 3 cut(s) 122, 168, 640
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 231
Tsp45I GTSAC 1 cut(s) 231
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 3 cut(s) 30, 356, 418
TspRI CASTG 1 cut(s) 181
XapI RAATTY 1 cut(s) 361
XmnI GAANNNNTTC 2 cut(s) 101, 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.