RchiOBHm_Chr7g0217971

DNA polymerase III, delta subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008140
Physical Location & Seq
Reverse (-)
35867105 .. 35867815
711 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 711 bp
ATGGTTATTAAGGCCATAACCAATGCAATTCAGCAGAATAAGATTGCACCGCTTTATCTTTTCTATGGTGCAAGAGGCTCCGGGAAGACATCTATTGCTATAATATTAGCATTGGCCTTGAACTGTGAATCCAGCTCATTTATCAAACCGTGCTGGAAATGCCAAGGATGCTCCAGAAGCCTTTCTATTATGGAGTTGTGTTCTGGAAGTACAGTTGCTGGATTCAAAAGGATTAGGAAACTCCTTCAAAGTATATCATCTTTGCAAGCAACACCAGGATTTAAGGTATTTATCCTTGAAGAGTGCGAATCGTTTAGTACAGAAGCATGGGACGAACTTTCAGGCATAGCAAATGGAGCTTACAATGCTGGTGTGGTATTCATCCTTATCACTACAAATGCAGACATGGTGCCTAGAACCATATCATCAAGATGTCAGAAATTCTTCTTTCCCAAACTCAGAGACACTGATATCATGCTCAAGTTGACTAGAATTGCAGCCCAGGAGGGTATAAGGATTGAAAAAGAAGCAATCAAACTGGTTACTACCAAAGCACAAGGATCTTTGCGAGAAGCTGAAAACATATTAGACCAGCTGATATTACTGGGGCCAAAGATAACAAGCTCCATGGCACAACAGCTTGTAGGTGGTCCTTTCTTTAAAACTTTCTACTCTCTCTTGGAGTTAAGTATACCTAATGGGGTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

236

Amino Acids

25.83

Weight (kDa)

9.2

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol3_delta2 PF13177 2 - 153 3.7e-20 DNA polymerase III, delta subunit
AAA PF00004 19 - 150 1.1e-08 ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid PF22608 158 - 203 1.5e-08 DNA polymerase III clamp loader subunit, ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 409
AccI GTMKAC 1 cut(s) 691
AciI CCGC 1 cut(s) 50
AclWI GGATC 1 cut(s) 568
AcsI RAATTY 1 cut(s) 440
AfaI GTAC 2 cut(s) 211, 319
AgsI TTSAA 5 cut(s) 121, 226, 248, 299, 521
AjnI CCWGG 2 cut(s) 274, 501
AloI GAACNNNNNNTCC 2 cut(s) 113, 145
AluBI AGCT 6 cut(s) 135, 359, 575, 595, 624, 640
AluI AGCT 6 cut(s) 135, 359, 575, 595, 624, 640
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 1 cut(s) 568
AlwNI CAGNNNCTG 1 cut(s) 218
AoxI GGCC 3 cut(s) 12, 114, 608
ApeKI GCWGC 1 cut(s) 497
ApoI RAATTY 1 cut(s) 440
Asp700I GAANNNNTTC 2 cut(s) 181, 443
AspS9I GGNCC 2 cut(s) 608, 650
AsuC2I CCSGG 1 cut(s) 82
AvaII GGWCC 1 cut(s) 650
BanI GGYRCC 1 cut(s) 409
BbsI GAAGAC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 509
BciT130I CCWGG 2 cut(s) 276, 503
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 2 cut(s) 414, 489
BisI GCNGC 1 cut(s) 498
BlsI GCNGC 1 cut(s) 499
Bme1390I CCNGG 3 cut(s) 82, 276, 503
Bme18I GGWCC 1 cut(s) 650
BmgT120I GGNCC 2 cut(s) 608, 650
BmiI GGNNCC 3 cut(s) 79, 411, 609
BmrFI CCNGG 3 cut(s) 82, 276, 503
BmrI ACTGGG 1 cut(s) 614
BmsI GCATC 1 cut(s) 158
BmuI ACTGGG 1 cut(s) 614
BpiI GAAGAC 1 cut(s) 92
BpmI CTGGAG 1 cut(s) 157
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 82
BsaJI CCNNGG 3 cut(s) 163, 501, 627
Bse1I ACTGG 2 cut(s) 543, 609
BseBI CCWGG 2 cut(s) 276, 503
BseDI CCNNGG 3 cut(s) 163, 501, 627
BseGI GGATG 2 cut(s) 173, 381
BseMII CTCAG 1 cut(s) 472
BseNI ACTGG 2 cut(s) 543, 609
BseXI GCAGC 1 cut(s) 509
BshFI GGCC 3 cut(s) 14, 116, 610
BshNI GGYRCC 1 cut(s) 409
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 1 cut(s) 344
BsmAI GTCTC 1 cut(s) 456
BsmFI GGGAC 1 cut(s) 344
BsnI GGCC 3 cut(s) 14, 116, 610
Bsp143I GATC 1 cut(s) 560
Bsp19I CCATGG 1 cut(s) 627
BspACI CCGC 1 cut(s) 50
BspANI GGCC 3 cut(s) 14, 116, 610
BspCNI CTCAG 1 cut(s) 471
BspLI GGNNCC 3 cut(s) 79, 411, 609
BspPI GGATC 1 cut(s) 568
BspT107I GGYRCC 1 cut(s) 409
BsrI ACTGG 2 cut(s) 543, 609
BssECI CCNNGG 3 cut(s) 163, 501, 627
BssMI GATC 1 cut(s) 560
BssNAI GTATAC 1 cut(s) 692
BssT1I CCWWGG 2 cut(s) 163, 627
Bst1107I GTATAC 1 cut(s) 692
Bst2UI CCWGG 2 cut(s) 276, 503
Bst4CI ACNGT 3 cut(s) 125, 150, 214
Bst6I CTCTTC 1 cut(s) 294
BstC8I GCNNGC 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 458
BstDSI CCRYGG 1 cut(s) 627
BstF5I GGATG 2 cut(s) 173, 381
BstKTI GATC 1 cut(s) 563
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 1 cut(s) 560
BstMWI GCNNNNNNNGC 5 cut(s) 159, 168, 177, 356, 365
BstNI CCWGG 2 cut(s) 276, 503
BstSCI CCNGG 3 cut(s) 80, 274, 501
BstV1I GCAGC 1 cut(s) 509
BstV2I GAAGAC 1 cut(s) 92
BstX2I RGATCY 1 cut(s) 560
BstYI RGATCY 1 cut(s) 560
BstZ17I GTATAC 1 cut(s) 692
BsuRI GGCC 3 cut(s) 14, 116, 610
BtgI CCRYGG 1 cut(s) 627
BtsCI GGATG 2 cut(s) 173, 381
BtsIMutI CAGTG 1 cut(s) 465
Cac8I GCNNGC 1 cut(s) 267
CaiI CAGNNNCTG 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 608, 650
Csp6I GTAC 2 cut(s) 210, 318
CviAII CATG 4 cut(s) 327, 406, 475, 628
CviQI GTAC 2 cut(s) 210, 318
DdeI CTNAG 1 cut(s) 458
DpnI GATC 1 cut(s) 562
DpnII GATC 1 cut(s) 560
DraI TTTAAA 1 cut(s) 661
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Eco130I CCWWGG 2 cut(s) 163, 627
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 650
EcoRII CCWGG 2 cut(s) 274, 501
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 2 cut(s) 163, 627
ErhI CCWWGG 2 cut(s) 163, 627
FaeI CATG 4 cut(s) 330, 409, 478, 631
FaqI GGGAC 1 cut(s) 344
FatI CATG 4 cut(s) 326, 405, 474, 627
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 1 cut(s) 498
FokI GGATG 2 cut(s) 180, 368
Fsp4HI GCNGC 1 cut(s) 498
FspBI CTAG 2 cut(s) 414, 489
GluI GCNGC 1 cut(s) 498
GsuI CTGGAG 1 cut(s) 157
HaeIII GGCC 3 cut(s) 14, 116, 610
HapII CCGG 1 cut(s) 81
Hin1II CATG 4 cut(s) 330, 409, 478, 631
HincII GTYRAC 2 cut(s) 486, 706
HindII GTYRAC 2 cut(s) 486, 706
HinfI GANTC 3 cut(s) 128, 222, 308
HpaII CCGG 1 cut(s) 81
Hpy166II GTNNAC 3 cut(s) 486, 692, 706
Hpy188I TCNGA 2 cut(s) 438, 461
Hpy188III TCNNGA 3 cut(s) 174, 204, 429
Hpy8I GTNNAC 3 cut(s) 486, 692, 706
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 3 cut(s) 125, 150, 214
HpyCH4V TGCA 6 cut(s) 26, 47, 71, 265, 401, 497
HpyF10VI GCNNNNNNNGC 5 cut(s) 159, 168, 177, 356, 365
HpyF3I CTNAG 1 cut(s) 458
Hsp92II CATG 4 cut(s) 330, 409, 478, 631
Kzo9I GATC 1 cut(s) 560
LmnI GCTCC 4 cut(s) 83, 176, 356, 629
Lsp1109I GCAGC 1 cut(s) 509
LweI GCATC 1 cut(s) 158
MaeI CTAG 2 cut(s) 414, 489
MaeIII GTNAC 1 cut(s) 541
MalI GATC 1 cut(s) 562
MboI GATC 1 cut(s) 560
MboII GAAGA 3 cut(s) 97, 311, 436
MflI RGATCY 1 cut(s) 560
MluCI AATT 3 cut(s) 27, 440, 492
MnlI CCTC 2 cut(s) 68, 499
MroXI GAANNNNTTC 2 cut(s) 181, 443
MseI TTAA 4 cut(s) 9, 282, 660, 686
MslI CAYNNNNRTG 1 cut(s) 430
MspA1I CMGCKG 1 cut(s) 595
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 3 cut(s) 82, 276, 503
MvaI CCWGG 2 cut(s) 276, 503
MwoI GCNNNNNNNGC 5 cut(s) 159, 168, 177, 356, 365
NciI CCSGG 1 cut(s) 82
NcoI CCATGG 1 cut(s) 627
NdeII GATC 1 cut(s) 560
NlaIII CATG 4 cut(s) 330, 409, 478, 631
NlaIV GGNNCC 3 cut(s) 79, 411, 609
PdmI GAANNNNTTC 2 cut(s) 181, 443
PfeI GAWTC 3 cut(s) 128, 222, 308
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 1 cut(s) 499
Psp6I CCWGG 2 cut(s) 274, 501
PspGI CCWGG 2 cut(s) 274, 501
PspN4I GGNNCC 3 cut(s) 79, 411, 609
PspPI GGNCC 2 cut(s) 608, 650
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 1 cut(s) 560
PvuII CAGCTG 1 cut(s) 595
RsaI GTAC 2 cut(s) 211, 319
RsaNI GTAC 2 cut(s) 210, 318
RseI CAYNNNNRTG 1 cut(s) 430
SaqAI TTAA 4 cut(s) 9, 282, 660, 686
SatI GCNGC 1 cut(s) 498
Sau3AI GATC 1 cut(s) 560
Sau96I GGNCC 2 cut(s) 608, 650
ScrFI CCNGG 3 cut(s) 82, 276, 503
SetI ASST 9 cut(s) 137, 288, 361, 577, 597, 626, 642, 649, 697
SfaNI GCATC 1 cut(s) 158
SinI GGWCC 1 cut(s) 650
SmiMI CAYNNNNRTG 1 cut(s) 430
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 3 cut(s) 27, 440, 492
SsiI CCGC 1 cut(s) 50
SspI AATATT 1 cut(s) 105
SspMI CTAG 2 cut(s) 414, 489
StyD4I CCNGG 3 cut(s) 80, 274, 501
StyI CCWWGG 2 cut(s) 163, 627
TaaI ACNGT 3 cut(s) 125, 150, 214
TasI AATT 3 cut(s) 27, 440, 492
TatI WGTACW 2 cut(s) 209, 317
TfiI GAWTC 3 cut(s) 128, 222, 308
Tru1I TTAA 4 cut(s) 9, 282, 660, 686
Tru9I TTAA 4 cut(s) 9, 282, 660, 686
TscAI CASTG 1 cut(s) 472
TseI GCWGC 1 cut(s) 497
TspDTI ATGAA 1 cut(s) 370
TspRI CASTG 1 cut(s) 472
VpaK11BI GGWCC 1 cut(s) 650
XapI RAATTY 1 cut(s) 440
XmiI GTMKAC 1 cut(s) 691
XmnI GAANNNNTTC 2 cut(s) 181, 443
XspI CTAG 2 cut(s) 414, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.