RchiOBHm_Chr5g0052761

Interconversion of serine and glycine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001957
Physical Location & Seq
Reverse (-)
54839236 .. 54840192
957 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 315 bp
ATGGCACACATCAGTGGATTGGTTGCGGCAGGTGTTATTCCATCTCCTTTTGAGTATGCAGATATTGTTACAACCACAACTCACAAGTCACTTCGTGGCCCTCGTGGGGCTATGATCTTCTTCAGGAAGGGGGTCAAAAATGTAAACAAGCAAGGACAAGAAGTTGTCTTTCCTGGACTCCAAGGTGGTCCACACAATCACACCATAGCTGGGTTAGCAGTTGCACTCAAGCAGGCCACAACTCCAGAGTTCAAAGCTTACCAAGAACAAGTTCTCAGTAATTCCGCAAAATTTGCAGAGCTTTATGCCCTCTGA

Protein Analysis

104

Amino Acids

11.14

Weight (kDa)

9.63

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SHMT PF00464 1 - 100 2.3e-48 Serine hydroxymethyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 20
Acc36I ACCTGC 1 cut(s) 20
AciI CCGC 2 cut(s) 26, 285
AcsI RAATTY 1 cut(s) 290
AcuI CTGAAG 1 cut(s) 106
AdeI CACNNNGTG 1 cut(s) 95
AfiI CCNNNNNNNGG 2 cut(s) 106, 210
AgsI TTSAA 1 cut(s) 253
AjnI CCWGG 1 cut(s) 172
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AleI CACNNNNGTG 1 cut(s) 12
AluBI AGCT 3 cut(s) 209, 257, 301
AluI AGCT 3 cut(s) 209, 257, 301
AoxI GGCC 2 cut(s) 97, 234
ApoI RAATTY 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 270
AspS9I GGNCC 2 cut(s) 98, 188
AvaII GGWCC 1 cut(s) 188
BauI CACGAG 1 cut(s) 102
BccI CCATC 1 cut(s) 49
BciT130I CCWGG 1 cut(s) 174
BfuAI ACCTGC 1 cut(s) 20
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 98, 188
BmrFI CCNGG 1 cut(s) 174
BpmI CTGGAG 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 181
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 210
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 181
BseLI CCNNNNNNNGG 2 cut(s) 106, 210
BseMII CTCAG 1 cut(s) 289
BseYI CCCAGC 1 cut(s) 209
BshFI GGCC 2 cut(s) 99, 236
BslI CCNNNNNNNGG 2 cut(s) 106, 210
BsnI GGCC 2 cut(s) 99, 236
Bsp143I GATC 1 cut(s) 114
BspACI CCGC 2 cut(s) 26, 285
BspANI GGCC 2 cut(s) 99, 236
BspCNI CTCAG 1 cut(s) 288
BspMI ACCTGC 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 1 cut(s) 114
BssSI CACGAG 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 181
Bst2BI CACGAG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 174
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 1 cut(s) 275
BstKTI GATC 1 cut(s) 117
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 2 cut(s) 215, 293
BstNI CCWGG 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 172
BsuRI GGCC 2 cut(s) 99, 236
BtsIMutI CAGTG 1 cut(s) 19
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 234
Cfr13I GGNCC 2 cut(s) 98, 188
CviJI RGCY 6 cut(s) 99, 110, 209, 236, 257, 301
CviKI_1 RGCY 6 cut(s) 99, 110, 209, 236, 257, 301
DdeI CTNAG 1 cut(s) 275
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
DraIII CACNNNGTG 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 181
FaiI YATR 4 cut(s) 57, 113, 206, 306
Fnu4HI GCNGC 1 cut(s) 27
Fsp4HI GCNGC 1 cut(s) 27
GluI GCNGC 1 cut(s) 27
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 2 cut(s) 99, 236
HindIII AAGCTT 1 cut(s) 255
HinfI GANTC 1 cut(s) 177
Hpy166II GTNNAC 2 cut(s) 145, 191
Hpy188I TCNGA 1 cut(s) 314
Hpy188III TCNNGA 2 cut(s) 124, 245
Hpy8I GTNNAC 2 cut(s) 145, 191
HpyAV CCTTC 1 cut(s) 121
HpyCH4V TGCA 3 cut(s) 59, 224, 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 293
HpyF3I CTNAG 1 cut(s) 275
Kzo9I GATC 1 cut(s) 114
LpnPI CCDG 7 cut(s) 15, 109, 159, 186, 195, 218, 258
MaeIII GTNAC 2 cut(s) 67, 87
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 2 cut(s) 109, 112
MluCI AATT 2 cut(s) 280, 290
MlyI GAGTC 1 cut(s) 171
MnlI CCTC 1 cut(s) 111
MroXI GAANNNNTTC 1 cut(s) 270
MslI CAYNNNNRTG 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 174
MvaI CCWGG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 215, 293
NdeII GATC 1 cut(s) 114
NmuCI GTSAC 1 cut(s) 87
OliI CACNNNNGTG 1 cut(s) 12
PaqCI CACCTGC 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 100
PdmI GAANNNNTTC 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 172
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 1 cut(s) 171
PpsI GAGTC 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 1 cut(s) 172
PspPI GGNCC 2 cut(s) 98, 188
RseI CAYNNNNRTG 1 cut(s) 12
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 2 cut(s) 98, 188
SchI GAGTC 1 cut(s) 171
ScrFI CCNGG 1 cut(s) 174
SetI ASST 5 cut(s) 34, 187, 211, 259, 303
SinI GGWCC 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 12
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 2 cut(s) 280, 290
SsiI CCGC 2 cut(s) 26, 285
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 1 cut(s) 181
TasI AATT 2 cut(s) 280, 290
TauI GCSGC 1 cut(s) 29
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 87
Tsp45I GTSAC 1 cut(s) 87
TspRI CASTG 1 cut(s) 19
VpaK11BI GGWCC 1 cut(s) 188
XapI RAATTY 1 cut(s) 290
XmnI GAANNNNTTC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.