RchiOBHm_Chr5g0083421

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005120
Physical Location & Seq
Reverse (-)
89093701 .. 89095687
1987 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 546 bp
ATGGCGCAGAACCTTCTCCGCCTCCGGCGAAGCCTAAGCAGCAGCCTCGTCGCAAATCTCCACCGCTCTCTATCGTCCACCTCCGCACTCTCCTCTGTCCCACGCTCCTCTTCCCCTTCCCCTTCCTCCTCCTCCGCCGTTGTTCAATCTCTCCAATTCCGCTTCCAATCTCGATCGTTCAGGTCGTCCTCCTTCTCTTCTGCACGGCCGCCTTACGGTAGCAACAACCAAAATGACGAGATTGGCCCCGACACCATACTCTTCGAAGGCTGCGATTACAACCACTGGCTCATCGTCATGGACTTCCCCAGAGATAACAAGCCTTCTCCCGAAGAGATGGTCAGAACTTATGAGGAAACCTGTGCCAGAGGCTTGAATATCAGTGTGGAAGAGGCAAAAAAGAAAATTTATGCTTGTAGCACAACAACGTATACAGGCTTTCAGGTGTTGATGACTGAGGAAGAGTCTGAGAAGTTTAATGGTGTACCTGGAGTTATTTTTGTGTTCCCGGATTCTTTATTTTTCTGCACGATACTGGAAAAATGA

Protein Analysis

181

Amino Acids

20.22

Weight (kDa)

6.14

Isoelectric Point (pI)

84.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 93 - 174 1.7e-31 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 66
AccI GTMKAC 1 cut(s) 431
AciI CCGC 6 cut(s) 19, 64, 84, 135, 160, 209
AcoI YGGCCR 1 cut(s) 206
AcsI RAATTY 1 cut(s) 405
AfaI GTAC 1 cut(s) 486
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 2 cut(s) 146, 376
AjnI CCWGG 1 cut(s) 487
AoxI GGCC 2 cut(s) 206, 244
ApeKI GCWGC 3 cut(s) 39, 42, 270
ApoI RAATTY 1 cut(s) 405
ArsI GACNNNNNNTTYG 2 cut(s) 324, 356
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 1 cut(s) 509
AsuII TTCGAA 1 cut(s) 264
BbvI GCAGC 3 cut(s) 51, 54, 257
BccI CCATC 1 cut(s) 331
BceAI ACGGC 2 cut(s) 122, 221
BcgI CGANNNNNNTGC 4 cut(s) 28, 31, 62, 65
BciT130I CCWGG 1 cut(s) 489
BcnI CCSGG 1 cut(s) 509
BisI GCNGC 4 cut(s) 40, 43, 209, 271
BlsI GCNGC 4 cut(s) 41, 44, 210, 272
Bme1390I CCNGG 2 cut(s) 489, 509
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 1 cut(s) 247
BmrFI CCNGG 2 cut(s) 489, 509
BpmI CTGGAG 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 35
Bpu14I TTCGAA 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 509
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 290, 540
BseBI CCWGG 1 cut(s) 489
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMII CTCAG 2 cut(s) 447, 459
BseNI ACTGG 2 cut(s) 290, 540
BseRI GAGGAG 4 cut(s) 82, 97, 118, 121
BseX3I CGGCCG 1 cut(s) 206
BseXI GCAGC 3 cut(s) 51, 54, 257
BsgI GTGCAG 2 cut(s) 186, 511
Bsh1285I CGRYCG 2 cut(s) 176, 209
BshFI GGCC 2 cut(s) 208, 246
BsiEI CGRYCG 2 cut(s) 176, 209
BsiSI CCGG 2 cut(s) 25, 509
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 2 cut(s) 208, 246
Bsp119I TTCGAA 1 cut(s) 264
Bsp143I GATC 1 cut(s) 173
BspACI CCGC 6 cut(s) 19, 64, 84, 135, 160, 209
BspANI GGCC 2 cut(s) 208, 246
BspCNI CTCAG 2 cut(s) 448, 460
BspLI GGNNCC 1 cut(s) 247
BspT104I TTCGAA 1 cut(s) 264
BsrBI CCGCTC 1 cut(s) 66
BsrI ACTGG 2 cut(s) 290, 540
BssMI GATC 1 cut(s) 173
BssNAI GTATAC 1 cut(s) 432
Bst1107I GTATAC 1 cut(s) 432
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 1 cut(s) 218
Bst6I CTCTTC 6 cut(s) 115, 202, 266, 327, 384, 456
BstBI TTCGAA 1 cut(s) 264
BstDEI CTNAG 3 cut(s) 35, 456, 468
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 176
BstMBI GATC 1 cut(s) 173
BstMCI CGRYCG 2 cut(s) 176, 209
BstMWI GCNNNNNNNGC 1 cut(s) 39
BstNI CCWGG 1 cut(s) 489
BstSCI CCNGG 2 cut(s) 487, 507
BstV1I GCAGC 3 cut(s) 51, 54, 257
BstZ17I GTATAC 1 cut(s) 432
BstZI CGGCCG 1 cut(s) 206
BsuRI GGCC 2 cut(s) 208, 246
BtsIMutI CAGTG 2 cut(s) 283, 388
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 245
Csp6I GTAC 1 cut(s) 485
CviAII CATG 1 cut(s) 298
CviJI RGCY 9 cut(s) 33, 45, 208, 246, 270, 289, 322, 372, 438
CviKI_1 RGCY 9 cut(s) 33, 45, 208, 246, 270, 289, 322, 372, 438
CviQI GTAC 1 cut(s) 485
DdeI CTNAG 3 cut(s) 35, 456, 468
DpnI GATC 1 cut(s) 175
DpnII GATC 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 206
EagI CGGCCG 1 cut(s) 206
Eam1104I CTCTTC 6 cut(s) 115, 202, 266, 327, 384, 456
EarI CTCTTC 6 cut(s) 115, 202, 266, 327, 384, 456
EciI GGCGGA 2 cut(s) 8, 124
EclXI CGGCCG 1 cut(s) 206
Eco52I CGGCCG 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 487
FaeI CATG 1 cut(s) 301
FaiI YATR 5 cut(s) 257, 299, 351, 411, 432
FaqI GGGAC 1 cut(s) 83
FatI CATG 1 cut(s) 297
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 4 cut(s) 40, 43, 209, 271
Fsp4HI GCNGC 4 cut(s) 40, 43, 209, 271
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 40, 43, 209, 271
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 2 cut(s) 208, 246
HapII CCGG 2 cut(s) 25, 509
HhaI GCGC 1 cut(s) 7
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 2 cut(s) 464, 512
HpaII CCGG 2 cut(s) 25, 509
Hpy166II GTNNAC 3 cut(s) 78, 432, 485
Hpy188I TCNGA 2 cut(s) 344, 469
Hpy188III TCNNGA 2 cut(s) 171, 329
Hpy8I GTNNAC 3 cut(s) 78, 432, 485
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 6 cut(s) 23, 126, 132, 202, 260, 333
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 2 cut(s) 203, 528
HpyF10VI GCNNNNNNNGC 1 cut(s) 39
HpyF3I CTNAG 3 cut(s) 35, 456, 468
HpySE526I ACGT 1 cut(s) 428
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 1 cut(s) 173
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 3 cut(s) 51, 54, 257
MaeII ACGT 1 cut(s) 428
MalI GATC 1 cut(s) 175
MbiI CCGCTC 1 cut(s) 66
MboI GATC 1 cut(s) 173
MboII GAAGA 6 cut(s) 102, 189, 253, 344, 401, 473
MluCI AATT 2 cut(s) 155, 405
MlyI GAGTC 1 cut(s) 473
MseI TTAA 1 cut(s) 477
MslI CAYNNNNRTG 1 cut(s) 296
MspI CCGG 2 cut(s) 25, 509
MspR9I CCNGG 2 cut(s) 489, 509
MvaI CCWGG 1 cut(s) 489
MwoI GCNNNNNNNGC 1 cut(s) 39
NciI CCSGG 1 cut(s) 509
NdeII GATC 1 cut(s) 173
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 1 cut(s) 247
NspV TTCGAA 1 cut(s) 264
PcsI WCGNNNNNNNCGW 2 cut(s) 182, 270
PfeI GAWTC 1 cut(s) 512
PfoI TCCNGGA 1 cut(s) 507
PkrI GCNGC 4 cut(s) 41, 44, 210, 272
Ple19I CGATCG 1 cut(s) 176
PleI GAGTC 1 cut(s) 472
PpsI GAGTC 1 cut(s) 472
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 1 cut(s) 245
PvuI CGATCG 1 cut(s) 176
RsaI GTAC 1 cut(s) 486
RsaNI GTAC 1 cut(s) 485
RseI CAYNNNNRTG 1 cut(s) 296
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 4 cut(s) 40, 43, 209, 271
Sau3AI GATC 1 cut(s) 173
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 2 cut(s) 489, 509
SetI ASST 7 cut(s) 15, 83, 185, 362, 431, 447, 490
SfuI TTCGAA 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 296
Sse9I AATT 2 cut(s) 155, 405
SsiI CCGC 6 cut(s) 19, 64, 84, 135, 160, 209
StyD4I CCNGG 2 cut(s) 487, 507
TaaI ACNGT 1 cut(s) 218
TaiI ACGT 1 cut(s) 431
TaqI TCGA 2 cut(s) 172, 264
TasI AATT 2 cut(s) 155, 405
TauI GCSGC 1 cut(s) 211
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 2 cut(s) 290, 388
TseI GCWGC 3 cut(s) 39, 42, 270
TspRI CASTG 2 cut(s) 290, 388
XapI RAATTY 1 cut(s) 405
XmiI GTMKAC 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.