RchiOBHm_Chr4g0406181

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008218
Physical Location & Seq
Forward (+)
27278503 .. 27282223
3721 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2796 bp
ATGGCTAGGGTTTTGGCTCTGTCTTCAGCAAATAATATTCCTTGTGTGGTTGGTGGGCTAGTTAGGACTGCTCAATCTAAAGAATTGGGTGGAAAAAAGAGTAGTAGAGTGAAGATGATGTGTACTTCTTCGCCAGTAGGAGGAGCACTTGCTTTGAGAATGAGGTGCATGAGTAGTAGTAGCACTAGTACTGCTTCTAGTTTCTCAGGACTCCGTAGGTTTAACTCCCTAGATAAGCTGGTGGCCACACCTAGCAGCCTACACCACGAGGAATACTCTTTTGGAATTGCAACCCCTTGGGGTTCCGAAAGTCGGAAGGGGGCTAGAAGTGGAAGAGCTAGCAGATGTGTACCTAAAGCCATGTTTGAGCGGTTCACAGACAAAGCAATTAAAGTGGTTCTGCTTGCACAAGAGGAATCAAGACGACTTGGTCACAATTTCGTGGGCACTGAGCAGATCCTTTTGGGTCTCATTGGTGAAGGAACTGGTCTTCCTTCCAAGGTTCTGAAATCCTCCATGGGAATTAATCTTAAAGAGGCACGAATTCAAGTGGAGAAGATAATTGGAAGGGGTTCTGGATTTATTGCTGTTGAGATCCCATTCACTTCTCGTGCCAAACGAGTTTTGGAACTCTCACTCGAAGAGGCTCGCCAACTTGGCCATAACTACATTGGATCCGAACACTTGCTTCTGGGACTGCTTCGAGAGGGGGAGGGTGTAGCAGCAGTTGTTCTAGCGAATTCTGGTGTCGATCCTTGTAATATTCGCACACAGGTTATCCATATGGTGGGCAGTGAGAACACAGCAGAAGCTGTTGTTGATCATAGGCGAGGGGATGATAAGACTAAAATGCCAACACTAGAGGAGTATGGGACCAATTTAACGAAGCTAGCAGAAGAGGGTAAGTTGGATCCTGTTGTTGGAAGAGAACAACAAATATCACGTGTCATCCAAATTTTGGGTAGGCGGACTAAGAATAATCCCTGCCTTATTGGAGAACCTGGTGTTGGGAAGACTGCAATTGCAGAAGGTCTTGCTCAACGTATTGCAAGTGGCGATGCTCCAGAAACAATAGCCAAAAAGACGGTTATAACTCTTGATACGGGTCTTCTTGTGTCTGGTACCAAGTACCGCGGAGAGTTTGAGGGAAGATTGAAGTTGCTAATGGAGGAATTAAAAAAAAGTGGTGGTGAAGTTATTCTGTTTATCGATGAGGTGCACACTTTAATTGGAGCAGGAGCAGCAGAAGGAGCAATTGATGCTGCAAATTTATTGAAGCCGGCCCTTGCAAGAGGTGAACTGCAGTGCATTGGAGCCACAACACTAGATGAATACAGAAAGCACATTGAAAAAGACCCAGCATTGGAAAGACGATTCCAACCAGTCAAAGTTCCTGAACCAACGGTGGATGAAACCATACAGGTTTTGAGAGGGCTTCGAGAACGATATGAGATTCACCACAAAGTGCAGTATAATGATGAGGCCCTGAAAGCAGCTGCGCAGCTATCATACCAATACATCAGCGATCGCTTTCTTCCTGATAAAGCAATTGACTTGCTCGATGAAGCTGGTTCTACAGTACGGCTCCGTCATGCACAGCTTCCTGAAGAAGCTCAAGAGCTTGACAATATTCTGAGGCAGATAACAAAAGAGAAGAATGAAGCTAGTCGTAGCCAAGATTTTGAGAAGGCTGGGAAATTACGCGACAGAGAATTGGACCTTAAGAAACAGATCTCAGCAGTAACAGAGAAAGGCAAGGAGATAAGTGAGGCATTGGATTATGTTAATGGTGGCCCTATTGTTACTGAATTGGATATTCAACATATTTTGTCTTGTTGGACTGGTATTCCAGTTGAGAAAGTGTCAACAAATGAATCTGATCGCCTCCTCAAGATGGAAGAGACGCTCCATAAGAGGGTTATTGGCCAGGATGAAGCAGTCAGGGCTATTAGCAGGGCTATTCGTCGAGCTCGTGTTGGACTGAAGAGTCCTAATCGGCCAATTGCCAGTTTCATCTTTTCTGGTCCAACTGGTGTTGGAAAATCTGAGCTCGCGAAAACTTTGGCGACTTGCTATTTTGGCTCTGAAGAATCGATGGTGAGGCTTGATATGAGTGAGTTCATGGAAAGGCACACTCTGGTTGAAGCCGTTCGTCGCCATCCATACACTCTGGTGCTTTTTGATGAGATTGAGAAGGCTCATCCTGATGTTTTCAACTTGATGCTTCAAATACTTGAGGATGGAAGATTGACTGACAGCAAGGGCAGAACAGTAGATTTCAAGAATACACTCATCATTATGACGTCTAATGTTGGAAGCAGTGTCATTGAGAAAGGAGGCGGTAACATGGGATTTGACCCGGCCAAGGATGAAGACAAGGATAGCAGTTACAAACAAATTAAGAGCTTGGTGATGGAGGAATTGAAACACTACTTTCGACCAGAGTTCTTAAATAGGTTGGATGAAATGATTGTTTTTCGGCAGCTGTGTAAGTTGGAGGTAAAGGAAATTGCAGATATAATGCTGAACGAGGTTTATGGAAGGCTCAAGTCCAAAGAGATAGAGCTTCGTGTGACAGAGAGGTTCAAAGACAAAGTGGTTGACGAAGGATACGACCCAAGCTATGGAGCAAGGCCTTTAAGAAGAGCCATAATGAGACTTTTGGAGGACAGCATGGCTGAAAAGATGCTGGCAAGGGAGATCAAAGAGCGTGATTCAGTTATTGTGGATGTTGATTCTGATGGGAATGTGATTGTGCTCATCAATGGCAGCGCTAAGACAGATATAAAGAAATAG

Protein Analysis

931

Amino Acids

103.11

Weight (kDa)

7.89

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 124 - 247 2.5e-28 Clp repeat (R) N-terminal domain
Clp_N PF02861 201 - 269 6.8e-15 Clp repeat (R) N-terminal domain
AAA_16 PF13191 305 - 351 9.6e-06 AAA ATPase domain
AAA PF00004 329 - 442 6.4e-12 ATPase family associated with various cellular activities (AAA)
AAA_lid_9 PF17871 469 - 569 2.4e-34 AAA lid domain
UVR PF02151 543 - 577 3e-09 UvrB/uvrC motif
AAA_2 PF07724 666 - 713 1.2e-10 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 671 - 773 6.5e-09 AAA domain (dynein-related subfamily)
AAA_2 PF07724 712 - 822 2.1e-38 AAA domain (Cdc48 subfamily)
ClpB_D2-small PF10431 830 - 909 2.1e-24 C-terminal, D2-small domain, of ClpB protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1091
AasI GACNNNNNNGTC 2 cut(s) 429, 1986
AatII GACGTC 1 cut(s) 2306
Acc16I TGCGCA 1 cut(s) 1500
Acc65I GGTACC 1 cut(s) 1121
AccB1I GGYRCC 1 cut(s) 1121
AccB7I CCANNNNNTGG 3 cut(s) 787, 958, 2624
AccBSI CCGCTC 1 cut(s) 370
AccII CGCG 3 cut(s) 1134, 1704, 2054
AciI CCGC 5 cut(s) 370, 967, 1132, 1134, 2340
AclWI GGATC 7 cut(s) 451, 589, 669, 682, 746, 905, 918
AcoI YGGCCR 5 cut(s) 243, 658, 1924, 1997, 2361
AcsI RAATTY 4 cut(s) 543, 739, 954, 1267
AcuI CTGAAG 4 cut(s) 9, 1626, 2003, 2106
AcvI CACGTG 1 cut(s) 944
AcyI GRCGYC 1 cut(s) 2303
AdeI CACNNNGTG 1 cut(s) 1465
AfaI GTAC 6 cut(s) 124, 190, 351, 1123, 1130, 1581
AfeI AGCGCT 1 cut(s) 2773
AflII CTTAAG 1 cut(s) 1721
AflIII ACRYGT 1 cut(s) 943
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 2 cut(s) 1000, 1926
AjuI GAANNNNNNNTTGG 4 cut(s) 264, 296, 990, 1022
AleI CACNNNNGTG 1 cut(s) 2171
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
Alw21I GWGCWC 5 cut(s) 148, 1221, 1972, 2052, 2760
Alw26I GTCTC 3 cut(s) 473, 1895, 2650
Alw44I GTGCAC 1 cut(s) 1217
AlwI GGATC 7 cut(s) 451, 589, 669, 682, 746, 905, 918
AlwNI CAGNNNCTG 1 cut(s) 812
Aor51HI AGCGCT 1 cut(s) 2773
AoxI GGCC 9 cut(s) 243, 658, 1281, 1482, 1792, 1924, 1997, 2361, 2633
ApaLI GTGCAC 1 cut(s) 1217
ApeKI GCWGC 9 cut(s) 255, 722, 1241, 1262, 1493, 1496, 1501, 2482, 2769
ApoI RAATTY 4 cut(s) 543, 739, 954, 1267
AseI ATTAAT 1 cut(s) 525
AsiSI GCGATCGC 1 cut(s) 1528
Asp700I GAANNNNTTC 3 cut(s) 571, 1197, 2148
Asp718I GGTACC 1 cut(s) 1121
AspLEI GCGC 2 cut(s) 1501, 2774
AspS9I GGNCC 6 cut(s) 873, 1282, 1483, 1717, 1793, 2024
AsuC2I CCSGG 1 cut(s) 2360
AsuHPI GGTGA 6 cut(s) 488, 1202, 1307, 1448, 2110, 2422
AsuNHI GCTAGC 2 cut(s) 338, 889
AvaII GGWCC 3 cut(s) 873, 1717, 2024
BaeGI GKGCMC 2 cut(s) 449, 1221
BalI TGGCCA 3 cut(s) 245, 660, 1926
BamHI GGATCC 2 cut(s) 674, 910
BanI GGYRCC 1 cut(s) 1121
BanII GRGCYC 2 cut(s) 1972, 2052
BauI CACGAG 3 cut(s) 266, 609, 1971
BbrPI CACGTG 1 cut(s) 944
BbsI GAAGAC 5 cut(s) 15, 482, 1019, 1100, 2379
Bbv12I GWGCWC 5 cut(s) 148, 1221, 1972, 2052, 2760
BbvI GCAGC 9 cut(s) 267, 734, 1249, 1253, 1483, 1505, 1513, 2494, 2781
BccI CCATC 6 cut(s) 1888, 2089, 2166, 2234, 2407, 2735
BceAI ACGGC 2 cut(s) 1598, 2132
BciT130I CCWGG 2 cut(s) 1002, 1928
BciVI GTATCC 1 cut(s) 2603
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 1 cut(s) 2360
BcoDI GTCTC 3 cut(s) 473, 1895, 2650
BcuI ACTAGT 1 cut(s) 185
BfmI CTRYAG 2 cut(s) 1301, 1575
BfoI RGCGCY 1 cut(s) 2775
BfrI CTTAAG 1 cut(s) 1721
BfuI GTATCC 1 cut(s) 2603
BglI GCCNNNNNGGC 1 cut(s) 657
BglII AGATCT 1 cut(s) 1731
BisI GCNGC 9 cut(s) 256, 723, 1242, 1263, 1494, 1497, 1502, 2483, 2770
BlsI GCNGC 9 cut(s) 257, 724, 1243, 1264, 1495, 1498, 1503, 2484, 2771
BmcAI AGTACT 1 cut(s) 190
Bme1390I CCNGG 3 cut(s) 1002, 1928, 2360
Bme18I GGWCC 3 cut(s) 873, 1717, 2024
BmgT120I GGNCC 6 cut(s) 873, 1282, 1483, 1717, 1793, 2024
BmiI GGNNCC 7 cut(s) 304, 676, 874, 912, 1123, 1315, 1586
BmrFI CCNGG 3 cut(s) 1002, 1928, 2360
BmsI GCATC 4 cut(s) 1048, 1249, 2211, 2676
BmtI GCTAGC 2 cut(s) 342, 893
BpiI GAAGAC 5 cut(s) 15, 482, 1019, 1100, 2379
BplI GAGNNNNNCTC 2 cut(s) 260, 292
BpmI CTGGAG 1 cut(s) 1047
BpuEI CTTGAG 4 cut(s) 1599, 1874, 2255, 2531
BpuMI CCSGG 1 cut(s) 2360
Bsa29I ATCGAT 2 cut(s) 1209, 2093
BsaAI YACGTR 1 cut(s) 944
BsaHI GRCGYC 1 cut(s) 2303
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 5 cut(s) 296, 498, 516, 1132, 2364
Bse118I RCCGGY 1 cut(s) 1279
Bse1I ACTGG 7 cut(s) 134, 490, 1382, 1846, 1850, 2007, 2035
BseBI CCWGG 2 cut(s) 1002, 1928
BseCI ATCGAT 2 cut(s) 1209, 2093
BseDI CCNNGG 5 cut(s) 296, 498, 516, 1132, 2364
BseMII CTCAG 5 cut(s) 219, 441, 1625, 1749, 2037
BseNI ACTGG 7 cut(s) 134, 490, 1382, 1846, 1850, 2007, 2035
BseRI GAGGAG 3 cut(s) 156, 878, 1877
BseSI GKGCMC 2 cut(s) 449, 1221
BseXI GCAGC 9 cut(s) 267, 734, 1249, 1253, 1483, 1505, 1513, 2494, 2781
BseYI CCCAGC 2 cut(s) 1357, 1691
BsgI GTGCAG 1 cut(s) 1487
Bsh1236I CGCG 3 cut(s) 1134, 1704, 2054
Bsh1285I CGRYCG 1 cut(s) 1528
BshFI GGCC 9 cut(s) 245, 660, 1283, 1484, 1794, 1926, 1999, 2363, 2635
BshNI GGYRCC 1 cut(s) 1121
BshVI ATCGAT 2 cut(s) 1209, 2093
BsiEI CGRYCG 1 cut(s) 1528
BsiHKAI GWGCWC 5 cut(s) 148, 1221, 1972, 2052, 2760
BsiSI CCGG 2 cut(s) 1280, 2360
BslFI GGGAC 2 cut(s) 708, 886
BsmAI GTCTC 3 cut(s) 473, 1895, 2650
BsmBI CGTCTC 1 cut(s) 1895
BsmFI GGGAC 2 cut(s) 708, 886
BsnI GGCC 9 cut(s) 245, 660, 1283, 1484, 1794, 1926, 1999, 2363, 2635
Bso31I GGTCTC 1 cut(s) 473
Bsp1286I GDGCHC 6 cut(s) 148, 449, 1221, 1972, 2052, 2760
Bsp19I CCATGG 1 cut(s) 516
Bsp68I TCGCGA 1 cut(s) 2054
BspACI CCGC 5 cut(s) 370, 967, 1132, 1134, 2340
BspANI GGCC 9 cut(s) 245, 660, 1283, 1484, 1794, 1926, 1999, 2363, 2635
BspCNI CTCAG 5 cut(s) 218, 442, 1626, 1748, 2038
BspDI ATCGAT 2 cut(s) 1209, 2093
BspFNI CGCG 3 cut(s) 1134, 1704, 2054
BspLI GGNNCC 7 cut(s) 304, 676, 874, 912, 1123, 1315, 1586
BspMAI CTGCAG 1 cut(s) 1305
BspOI GCTAGC 2 cut(s) 342, 893
BspPI GGATC 7 cut(s) 451, 589, 669, 682, 746, 905, 918
BspQI GCTCTTC 2 cut(s) 328, 2638
BspT107I GGYRCC 1 cut(s) 1121
BspTI CTTAAG 1 cut(s) 1721
BspTNI GGTCTC 1 cut(s) 473
BsrBI CCGCTC 1 cut(s) 370
BsrFI RCCGGY 1 cut(s) 1279
BsrI ACTGG 7 cut(s) 134, 490, 1382, 1846, 1850, 2007, 2035
BssAI RCCGGY 1 cut(s) 1279
BssECI CCNNGG 5 cut(s) 296, 498, 516, 1132, 2364
BssNI GRCGYC 1 cut(s) 2303
BssSI CACGAG 3 cut(s) 266, 609, 1971
BssT1I CCWWGG 4 cut(s) 296, 498, 516, 2364
Bst2BI CACGAG 3 cut(s) 266, 609, 1971
Bst2UI CCWGG 2 cut(s) 1002, 1928
Bst4CI ACNGT 4 cut(s) 1087, 1405, 1579, 2272
Bst6I CTCTTC 7 cut(s) 328, 636, 891, 919, 1893, 1979, 2638
BstACI GRCGYC 1 cut(s) 2303
BstAFI CTTAAG 1 cut(s) 1721
BstAPI GCANNNNNTGC 1 cut(s) 1259
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 7 cut(s) 340, 405, 649, 891, 1281, 2052, 2691
BstDEI CTNAG 7 cut(s) 205, 450, 972, 1634, 1735, 2046, 2775
BstDSI CCRYGG 2 cut(s) 516, 1132
BstFNI CGCG 3 cut(s) 1134, 1704, 2054
BstH2I RGCGCY 1 cut(s) 2775
BstHHI GCGC 2 cut(s) 1501, 2774
BstMAI GTCTC 3 cut(s) 473, 1895, 2650
BstMCI CGRYCG 1 cut(s) 1528
BstMWI GCNNNNNNNGC 7 cut(s) 657, 1241, 1250, 1259, 1490, 1943, 2079
BstNI CCWGG 2 cut(s) 1002, 1928
BstSCI CCNGG 3 cut(s) 1000, 1926, 2358
BstSFI CTRYAG 2 cut(s) 1301, 1575
BstSLI GKGCMC 2 cut(s) 449, 1221
BstUI CGCG 3 cut(s) 1134, 1704, 2054
BstV1I GCAGC 9 cut(s) 267, 734, 1249, 1253, 1483, 1505, 1513, 2494, 2781
BstV2I GAAGAC 5 cut(s) 15, 482, 1019, 1100, 2379
BstX2I RGATCY 5 cut(s) 456, 594, 674, 910, 1731
BstYI RGATCY 5 cut(s) 456, 594, 674, 910, 1731
Bsu15I ATCGAT 2 cut(s) 1209, 2093
BsuI GTATCC 1 cut(s) 2603
BsuRI GGCC 9 cut(s) 245, 660, 1283, 1484, 1794, 1926, 1999, 2363, 2635
BsuTUI ATCGAT 2 cut(s) 1209, 2093
BtgI CCRYGG 2 cut(s) 516, 1132
BtgZI GCGATG 1 cut(s) 1071
BtsI GCAGTG 3 cut(s) 799, 1310, 2326
BtsIMutI CAGTG 4 cut(s) 447, 799, 1310, 2326
BtuMI TCGCGA 1 cut(s) 2054
Cac8I GCNNGC 7 cut(s) 340, 405, 649, 891, 1281, 2052, 2691
CaiI CAGNNNCTG 1 cut(s) 812
CfoI GCGC 2 cut(s) 1501, 2774
Cfr10I RCCGGY 1 cut(s) 1279
Cfr13I GGNCC 6 cut(s) 873, 1282, 1483, 1717, 1793, 2024
Cfr42I CCGCGG 1 cut(s) 1135
ClaI ATCGAT 2 cut(s) 1209, 2093
CseI GACGC 1 cut(s) 1912
CsiI ACCWGGT 1 cut(s) 1000
Csp6I GTAC 6 cut(s) 123, 189, 350, 1122, 1129, 1580
CspCI CAANNNNNGTGG 2 cut(s) 375, 410
CviAII CATG 7 cut(s) 169, 361, 517, 1592, 2122, 2347, 2674
CviQI GTAC 6 cut(s) 123, 189, 350, 1122, 1129, 1580
DdeI CTNAG 7 cut(s) 205, 450, 972, 1634, 1735, 2046, 2775
DraIII CACNNNGTG 1 cut(s) 1465
DrdI GACNNNNNNGTC 2 cut(s) 429, 1986
DseDI GACNNNNNNGTC 2 cut(s) 429, 1986
EaeI YGGCCR 5 cut(s) 243, 658, 1924, 1997, 2361
Eam1104I CTCTTC 7 cut(s) 328, 636, 891, 919, 1893, 1979, 2638
EarI CTCTTC 7 cut(s) 328, 636, 891, 919, 1893, 1979, 2638
EciI GGCGGA 1 cut(s) 982
Ecl136II GAGCTC 2 cut(s) 1970, 2050
Eco130I CCWWGG 4 cut(s) 296, 498, 516, 2364
Eco147I AGGCCT 1 cut(s) 2635
Eco24I GRGCYC 2 cut(s) 1972, 2052
Eco31I GGTCTC 1 cut(s) 473
Eco47I GGWCC 3 cut(s) 873, 1717, 2024
Eco47III AGCGCT 1 cut(s) 2773
Eco53kI GAGCTC 2 cut(s) 1970, 2050
Eco57I CTGAAG 4 cut(s) 9, 1626, 2003, 2106
Eco72I CACGTG 1 cut(s) 944
EcoICRI GAGCTC 2 cut(s) 1970, 2050
EcoO109I RGGNCCY 1 cut(s) 1483
EcoRI GAATTC 2 cut(s) 543, 739
EcoRII CCWGG 2 cut(s) 1000, 1926
EcoT14I CCWWGG 4 cut(s) 296, 498, 516, 2364
EcoT38I GRGCYC 2 cut(s) 1972, 2052
ErhI CCWWGG 4 cut(s) 296, 498, 516, 2364
Esp3I CGTCTC 1 cut(s) 1895
FaeI CATG 7 cut(s) 172, 364, 520, 1595, 2125, 2350, 2677
FaqI GGGAC 2 cut(s) 708, 886
FatI CATG 7 cut(s) 168, 360, 516, 1591, 2121, 2346, 2673
FauNDI CATATG 1 cut(s) 783
FbaI TGATCA 1 cut(s) 820
Fnu4HI GCNGC 9 cut(s) 256, 723, 1242, 1263, 1494, 1497, 1502, 2483, 2770
FriOI GRGCYC 2 cut(s) 1972, 2052
Fsp4HI GCNGC 9 cut(s) 256, 723, 1242, 1263, 1494, 1497, 1502, 2483, 2770
FspI TGCGCA 1 cut(s) 1500
GlaI GCGC 2 cut(s) 1500, 2773
GluI GCNGC 9 cut(s) 256, 723, 1242, 1263, 1494, 1497, 1502, 2483, 2770
GsaI CCCAGC 2 cut(s) 1361, 1695
GsuI CTGGAG 1 cut(s) 1047
HaeII RGCGCY 1 cut(s) 2775
HaeIII GGCC 9 cut(s) 245, 660, 1283, 1484, 1794, 1926, 1999, 2363, 2635
HapII CCGG 2 cut(s) 1280, 2360
HgaI GACGC 1 cut(s) 1912
HhaI GCGC 2 cut(s) 1501, 2774
Hin1I GRCGYC 1 cut(s) 2303
Hin1II CATG 7 cut(s) 172, 364, 520, 1595, 2125, 2350, 2677
Hin6I GCGC 2 cut(s) 1499, 2772
HinP1I GCGC 2 cut(s) 1499, 2772
HincII GTYRAC 2 cut(s) 1866, 2602
HindII GTYRAC 2 cut(s) 1866, 2602
HinfI GANTC 9 cut(s) 210, 416, 1374, 1453, 1874, 1987, 2090, 2714, 2735
HpaII CCGG 2 cut(s) 1280, 2360
HphI GGTGA 6 cut(s) 488, 1202, 1307, 1448, 2110, 2422
Hpy166II GTNNAC 7 cut(s) 123, 350, 375, 1219, 1298, 1866, 2602
Hpy188I TCNGA 9 cut(s) 307, 315, 507, 679, 1635, 1879, 2047, 2086, 2740
Hpy8I GTNNAC 7 cut(s) 123, 350, 375, 1219, 1298, 1866, 2602
Hpy99I CGWCG 2 cut(s) 1968, 2157
HpyCH4III ACNGT 4 cut(s) 1087, 1405, 1579, 2272
HpyCH4IV ACGT 3 cut(s) 943, 1042, 2303
HpyF10VI GCNNNNNNNGC 7 cut(s) 657, 1241, 1250, 1259, 1490, 1943, 2079
HpyF3I CTNAG 7 cut(s) 205, 450, 972, 1634, 1735, 2046, 2775
HpySE526I ACGT 3 cut(s) 943, 1042, 2303
Hsp92I GRCGYC 1 cut(s) 2303
Hsp92II CATG 7 cut(s) 172, 364, 520, 1595, 2125, 2350, 2677
HspAI GCGC 2 cut(s) 1499, 2772
KpnI GGTACC 1 cut(s) 1125
KroI GCCGGC 1 cut(s) 1279
KroNI GCCGGC 1 cut(s) 1281
Ksp22I TGATCA 1 cut(s) 820
KspI CCGCGG 1 cut(s) 1135
LguI GCTCTTC 2 cut(s) 328, 2638
LmnI GCTCC 9 cut(s) 143, 1066, 1232, 1238, 1250, 1313, 1590, 1911, 2627
Lsp1109I GCAGC 9 cut(s) 267, 734, 1249, 1253, 1483, 1505, 1513, 2494, 2781
LweI GCATC 4 cut(s) 1048, 1249, 2211, 2676
MabI ACCWGGT 1 cut(s) 1000
MaeII ACGT 3 cut(s) 943, 1042, 2303
MaeIII GTNAC 6 cut(s) 431, 1741, 1801, 2342, 2387, 2572
MbiI CCGCTC 1 cut(s) 370
MfeI CAATTG 4 cut(s) 1020, 1254, 1548, 2001
MflI RGATCY 5 cut(s) 456, 594, 674, 910, 1731
MhlI GDGCHC 6 cut(s) 148, 449, 1221, 1972, 2052, 2760
MlsI TGGCCA 3 cut(s) 245, 660, 1926
MluNI TGGCCA 3 cut(s) 245, 660, 1926
MlyI GAGTC 2 cut(s) 204, 1996
Mox20I TGGCCA 3 cut(s) 245, 660, 1926
MroNI GCCGGC 1 cut(s) 1279
MroXI GAANNNNTTC 3 cut(s) 571, 1197, 2148
MscI TGGCCA 3 cut(s) 245, 660, 1926
MslI CAYNNNNRTG 3 cut(s) 2171, 2205, 2297
Msp20I TGGCCA 3 cut(s) 245, 660, 1926
MspA1I CMGCKG 3 cut(s) 1134, 1496, 2485
MspCI CTTAAG 1 cut(s) 1721
MspI CCGG 2 cut(s) 1280, 2360
MspR9I CCNGG 3 cut(s) 1002, 1928, 2360
MunI CAATTG 4 cut(s) 1020, 1254, 1548, 2001
MvaI CCWGG 2 cut(s) 1002, 1928
MvnI CGCG 3 cut(s) 1134, 1704, 2054
MwoI GCNNNNNNNGC 7 cut(s) 657, 1241, 1250, 1259, 1490, 1943, 2079
NaeI GCCGGC 1 cut(s) 1281
NciI CCSGG 1 cut(s) 2360
NcoI CCATGG 1 cut(s) 516
NdeI CATATG 1 cut(s) 783
NgoMIV GCCGGC 1 cut(s) 1279
NheI GCTAGC 2 cut(s) 338, 889
NlaIII CATG 7 cut(s) 172, 364, 520, 1595, 2125, 2350, 2677
NlaIV GGNNCC 7 cut(s) 304, 676, 874, 912, 1123, 1315, 1586
NmuCI GTSAC 2 cut(s) 431, 2572
NruI TCGCGA 1 cut(s) 2054
NsbI TGCGCA 1 cut(s) 1500
OliI CACNNNNGTG 1 cut(s) 2171
PceI AGGCCT 1 cut(s) 2635
PciSI GCTCTTC 2 cut(s) 328, 2638
PcsI WCGNNNNNNNCGW 3 cut(s) 616, 1969, 2610
PdiI GCCGGC 1 cut(s) 1281
PdmI GAANNNNTTC 3 cut(s) 571, 1197, 2148
PfeI GAWTC 7 cut(s) 416, 1374, 1453, 1874, 2090, 2714, 2735
PflFI GACNNNGTC 1 cut(s) 429
PflMI CCANNNNNTGG 3 cut(s) 787, 958, 2624
PkrI GCNGC 9 cut(s) 257, 724, 1243, 1264, 1495, 1498, 1503, 2484, 2771
Ple19I CGATCG 1 cut(s) 1528
PleI GAGTC 2 cut(s) 204, 1995
PmaCI CACGTG 1 cut(s) 944
PmlI CACGTG 1 cut(s) 944
PpsI GAGTC 2 cut(s) 204, 1995
Ppu21I YACGTR 1 cut(s) 944
PshBI ATTAAT 1 cut(s) 525
PsiI TTATAA 1 cut(s) 1091
Psp124BI GAGCTC 2 cut(s) 1972, 2052
Psp6I CCWGG 2 cut(s) 1000, 1926
PspCI CACGTG 1 cut(s) 944
PspFI CCCAGC 2 cut(s) 1357, 1691
PspGI CCWGG 2 cut(s) 1000, 1926
PspN4I GGNNCC 7 cut(s) 304, 676, 874, 912, 1123, 1315, 1586
PspPI GGNCC 6 cut(s) 873, 1282, 1483, 1717, 1793, 2024
PstI CTGCAG 1 cut(s) 1305
PstNI CAGNNNCTG 1 cut(s) 812
PsuI RGATCY 5 cut(s) 456, 594, 674, 910, 1731
PsyI GACNNNGTC 1 cut(s) 429
PvuI CGATCG 1 cut(s) 1528
PvuII CAGCTG 2 cut(s) 1496, 2485
RgaI GCGATCGC 1 cut(s) 1528
RruI TCGCGA 1 cut(s) 2054
RsaI GTAC 6 cut(s) 124, 190, 351, 1123, 1130, 1581
RsaNI GTAC 6 cut(s) 123, 189, 350, 1122, 1129, 1580
RseI CAYNNNNRTG 3 cut(s) 2171, 2205, 2297
SacI GAGCTC 2 cut(s) 1972, 2052
SacII CCGCGG 1 cut(s) 1135
SapI GCTCTTC 2 cut(s) 328, 2638
SatI GCNGC 9 cut(s) 256, 723, 1242, 1263, 1494, 1497, 1502, 2483, 2770
Sau96I GGNCC 6 cut(s) 873, 1282, 1483, 1717, 1793, 2024
ScaI AGTACT 1 cut(s) 190
SchI GAGTC 2 cut(s) 204, 1996
ScrFI CCNGG 3 cut(s) 1002, 1928, 2360
SduI GDGCHC 6 cut(s) 148, 449, 1221, 1972, 2052, 2760
SexAI ACCWGGT 1 cut(s) 1000
SfaAI GCGATCGC 1 cut(s) 1528
SfaNI GCATC 4 cut(s) 1048, 1249, 2211, 2676
SfcI CTRYAG 2 cut(s) 1301, 1575
Sfr303I CCGCGG 1 cut(s) 1135
SgfI GCGATCGC 1 cut(s) 1528
SgrBI CCGCGG 1 cut(s) 1135
SinI GGWCC 3 cut(s) 873, 1717, 2024
SmiMI CAYNNNNRTG 3 cut(s) 2171, 2205, 2297
SmlI CTYRAG 5 cut(s) 1614, 1721, 1889, 2234, 2546
SmoI CTYRAG 5 cut(s) 1614, 1721, 1889, 2234, 2546
SpeI ACTAGT 1 cut(s) 185
SseBI AGGCCT 1 cut(s) 2635
SsiI CCGC 5 cut(s) 370, 967, 1132, 1134, 2340
SspI AATATT 3 cut(s) 37, 763, 1630
SstI GAGCTC 2 cut(s) 1972, 2052
StuI AGGCCT 1 cut(s) 2635
StyD4I CCNGG 3 cut(s) 1000, 1926, 2358
StyI CCWWGG 4 cut(s) 296, 498, 516, 2364
TaaI ACNGT 4 cut(s) 1087, 1405, 1579, 2272
TaiI ACGT 3 cut(s) 946, 1045, 2306
TaqI TCGA 9 cut(s) 639, 703, 750, 1209, 1438, 1560, 1966, 2093, 2437
TatI WGTACW 2 cut(s) 122, 188
TfiI GAWTC 7 cut(s) 416, 1374, 1453, 1874, 2090, 2714, 2735
TscAI CASTG 4 cut(s) 454, 799, 1310, 2326
TseFI GTSAC 2 cut(s) 431, 2572
TseI GCWGC 9 cut(s) 255, 722, 1241, 1262, 1493, 1496, 1501, 2482, 2769
Tsp45I GTSAC 2 cut(s) 431, 2572
TspGWI ACGGA 2 cut(s) 203, 1577
TspRI CASTG 4 cut(s) 454, 799, 1310, 2326
Tth111I GACNNNGTC 1 cut(s) 429
Van91I CCANNNNNTGG 3 cut(s) 787, 958, 2624
Vha464I CTTAAG 1 cut(s) 1721
VneI GTGCAC 1 cut(s) 1217
VpaK11BI GGWCC 3 cut(s) 873, 1717, 2024
VspI ATTAAT 1 cut(s) 525
XapI RAATTY 4 cut(s) 543, 739, 954, 1267
XcmI CCANNNNNNNNNTGG 2 cut(s) 622, 668
XmnI GAANNNNTTC 3 cut(s) 571, 1197, 2148
ZraI GACGTC 1 cut(s) 2304
ZrmI AGTACT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.