RchiOBHm_Chr2g0089431

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006633
Physical Location & Seq
Reverse (-)
3657169 .. 3660268
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 948 bp
ATGAAGGGGGAAGTGCAGCTAGAACCAGTTGTTGCGGAACGGGAACCGAACCCTAGTAGTGACTTGGACCCTCTGCTACAGAACACCAACCAGGACGACGACGATGAGTCGTCGTCTCCGGCGAGCTCCAGTGAGATTAGGAATGAAGACCTTGAGAATGGCTCCTCTATTGCTTGTTGTCGCATTTGCCTTGAAAGCGATGCTGAACCAGGGGATGAGTTGATTTCACCATGCTTGTGCAAAGGCACACAGCAGTTTGTGCATAGCTCCTGTCTTGATCACTGGCGCTCTGTTAAGGAAGGATTTGCTTTCTCGCACTGCACAACTTGCAAAGCCCAATTTCATCTTCGAGTTGAATCATATGAGGATAACTCTTGGCGTAAAATAAAATTCAGAATTTTTGTGGCAAGGGATGTTTTCCTTGTATTTGTGGCTGTACAAACAATAATTACTGCAATTGGTGGCTTTGCATATCTGATGGACAAAGATGGCGCCTTCAGGAATTCATTCAGTGATGGTTGGGATCGTATCTTGTCAAAACACCCTATACCATTTTATTATTGCATAGGGGTCTTGGCGTTTTTTGTGCTGCTTGGGTTTTTTGGGCTTATACTACACTGTTCAGCCTTCAATAGCAATGACCCACGGATGGCTGGCTGCCAGAACTGTTGTTATGGATGGGGAATATTGGATTGTTTCCCTGCGTCTATGGAGGCATGCTTTGCCCTGGTTATTGTTTTTGTTGTCATCTTTGCTATTCTTGGCATAGCTTATGGTTTCCTGGCTGCTACTATGGCTATTCAGAGAATCTGGCAGAGACATTACCACATCCTGACCAAGAGGGAACTCACGAAGGAGTACATAGTGGAGGACCTTCATGGTTGTTACACCCCACCAAAGTTGGATCCTGAACATGAAGAGCGTCTGAAAATGCTCAAGTTATTGTAG

Protein Analysis

315

Amino Acids

35.63

Weight (kDa)

5.21

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 60 - 110 7.3e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 491
AciI CCGC 1 cut(s) 35
AclWI GGATC 3 cut(s) 531, 899, 912
AcsI RAATTY 3 cut(s) 389, 396, 502
AcuI CTGAAG 1 cut(s) 481
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 2 cut(s) 438, 860
AfiI CCNNNNNNNGG 1 cut(s) 649
AgsI TTSAA 3 cut(s) 194, 356, 631
AhdI GACNNNNNGTC 1 cut(s) 106
AjnI CCWGG 4 cut(s) 90, 208, 726, 780
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AluBI AGCT 4 cut(s) 19, 126, 267, 770
AluI AGCT 4 cut(s) 19, 126, 267, 770
Alw21I GWGCWC 1 cut(s) 128
Alw26I GTCTC 2 cut(s) 120, 811
AlwI GGATC 3 cut(s) 531, 899, 912
ApeKI GCWGC 4 cut(s) 16, 589, 657, 785
ApoI RAATTY 3 cut(s) 389, 396, 502
Asp700I GAANNNNTTC 1 cut(s) 506
AspLEI GCGC 2 cut(s) 288, 494
AspS9I GGNCC 2 cut(s) 67, 871
AsuHPI GGTGA 1 cut(s) 219
AvaII GGWCC 2 cut(s) 67, 871
BamHI GGATCC 1 cut(s) 904
BanI GGYRCC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 128
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 128
BbvI GCAGC 4 cut(s) 28, 576, 644, 772
BccI CCATC 5 cut(s) 472, 482, 509, 643, 672
BciT130I CCWGG 4 cut(s) 92, 210, 728, 782
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 2 cut(s) 120, 811
BfaI CTAG 2 cut(s) 20, 54
BfmI CTRYAG 1 cut(s) 77
BfoI RGCGCY 2 cut(s) 289, 495
BisI GCNGC 4 cut(s) 17, 590, 658, 786
BlsI GCNGC 4 cut(s) 18, 591, 659, 787
Bme1390I CCNGG 4 cut(s) 92, 210, 728, 782
Bme18I GGWCC 2 cut(s) 67, 871
BmeRI GACNNNNNGTC 1 cut(s) 106
BmgT120I GGNCC 2 cut(s) 67, 871
BmiI GGNNCC 5 cut(s) 45, 69, 163, 493, 906
BmrFI CCNGG 4 cut(s) 92, 210, 728, 782
BmsI GCATC 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 153
BplI GAGNNNNNCTC 4 cut(s) 146, 178, 356, 388
BpmI CTGGAG 1 cut(s) 112
BpuEI CTTGAG 2 cut(s) 173, 920
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 3 cut(s) 209, 644, 726
Bsc4I CCNNNNNNNGG 1 cut(s) 649
Bse1I ACTGG 3 cut(s) 26, 129, 287
Bse3DI GCAATG 1 cut(s) 643
BseBI CCWGG 4 cut(s) 92, 210, 728, 782
BseDI CCNNGG 3 cut(s) 209, 644, 726
BseGI GGATG 5 cut(s) 220, 418, 654, 683, 828
BseLI CCNNNNNNNGG 1 cut(s) 649
BseMI GCAATG 1 cut(s) 643
BseNI ACTGG 3 cut(s) 26, 129, 287
BseRI GAGGAG 1 cut(s) 154
BseXI GCAGC 4 cut(s) 28, 576, 644, 772
BsgI GTGCAG 2 cut(s) 35, 304
BshNI GGYRCC 1 cut(s) 491
BsiHKAI GWGCWC 1 cut(s) 128
BsiSI CCGG 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 649
BsmAI GTCTC 2 cut(s) 120, 811
BsmBI CGTCTC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 128
Bsp1407I TGTACA 1 cut(s) 436
Bsp143I GATC 3 cut(s) 277, 523, 904
BspACI CCGC 1 cut(s) 35
BspLI GGNNCC 5 cut(s) 45, 69, 163, 493, 906
BspPI GGATC 3 cut(s) 531, 899, 912
BspQI GCTCTTC 1 cut(s) 912
BspT107I GGYRCC 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 643
BsrGI TGTACA 1 cut(s) 436
BsrI ACTGG 3 cut(s) 26, 129, 287
BssECI CCNNGG 3 cut(s) 209, 644, 726
BssMI GATC 3 cut(s) 277, 523, 904
BssNI GRCGYC 1 cut(s) 492
Bst2UI CCWGG 4 cut(s) 92, 210, 728, 782
Bst4CI ACNGT 2 cut(s) 620, 668
Bst6I CTCTTC 1 cut(s) 912
BstACI GRCGYC 1 cut(s) 492
BstAPI GCANNNNNTGC 3 cut(s) 259, 327, 722
BstAUI TGTACA 1 cut(s) 436
BstC8I GCNNGC 3 cut(s) 124, 655, 718
BstDSI CCRYGG 1 cut(s) 644
BstF5I GGATG 5 cut(s) 220, 418, 654, 683, 828
BstH2I RGCGCY 2 cut(s) 289, 495
BstHHI GCGC 2 cut(s) 288, 494
BstKTI GATC 3 cut(s) 280, 526, 907
BstMAI GTCTC 2 cut(s) 120, 811
BstMBI GATC 3 cut(s) 277, 523, 904
BstMWI GCNNNNNNNGC 5 cut(s) 195, 259, 327, 722, 794
BstNI CCWGG 4 cut(s) 92, 210, 728, 782
BstNSI RCATGY 1 cut(s) 720
BstSCI CCNGG 4 cut(s) 90, 208, 726, 780
BstSFI CTRYAG 1 cut(s) 77
BstV1I GCAGC 4 cut(s) 28, 576, 644, 772
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 1 cut(s) 904
BstYI RGATCY 1 cut(s) 904
BtgI CCRYGG 1 cut(s) 644
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 5 cut(s) 220, 418, 654, 683, 828
BtsI GCAGTG 1 cut(s) 316
BtsIMutI CAGTG 5 cut(s) 136, 280, 316, 517, 616
Cac8I GCNNGC 3 cut(s) 124, 655, 718
CfoI GCGC 2 cut(s) 288, 494
Cfr13I GGNCC 2 cut(s) 67, 871
CseI GACGC 2 cut(s) 693, 911
Csp6I GTAC 2 cut(s) 437, 859
CspCI CAANNNNNGTGG 2 cut(s) 882, 917
CviAII CATG 4 cut(s) 231, 717, 878, 914
CviQI GTAC 2 cut(s) 437, 859
DinI GGCGCC 1 cut(s) 493
DpnI GATC 3 cut(s) 279, 525, 906
DpnII GATC 3 cut(s) 277, 523, 904
DriI GACNNNNNGTC 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 912
Eam1105I GACNNNNNGTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 912
Ecl136II GAGCTC 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 128
Eco47I GGWCC 2 cut(s) 67, 871
Eco53kI GAGCTC 1 cut(s) 126
Eco57I CTGAAG 1 cut(s) 481
EcoICRI GAGCTC 1 cut(s) 126
EcoO109I RGGNCCY 1 cut(s) 871
EcoRI GAATTC 1 cut(s) 502
EcoRII CCWGG 4 cut(s) 90, 208, 726, 780
EcoT38I GRGCYC 1 cut(s) 128
EgeI GGCGCC 1 cut(s) 493
EheI GGCGCC 1 cut(s) 493
Esp3I CGTCTC 1 cut(s) 120
FaeI CATG 4 cut(s) 234, 720, 881, 917
FatI CATG 4 cut(s) 230, 716, 877, 913
FauNDI CATATG 1 cut(s) 361
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 4 cut(s) 17, 590, 658, 786
FokI GGATG 5 cut(s) 227, 425, 661, 690, 815
FriOI GRGCYC 1 cut(s) 128
Fsp4HI GCNGC 4 cut(s) 17, 590, 658, 786
FspBI CTAG 2 cut(s) 20, 54
GlaI GCGC 2 cut(s) 287, 493
GluI GCNGC 4 cut(s) 17, 590, 658, 786
GsuI CTGGAG 1 cut(s) 112
HaeII RGCGCY 2 cut(s) 289, 495
HapII CCGG 1 cut(s) 119
HgaI GACGC 2 cut(s) 693, 911
HhaI GCGC 2 cut(s) 288, 494
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 4 cut(s) 234, 720, 881, 917
Hin6I GCGC 2 cut(s) 286, 492
HinP1I GCGC 2 cut(s) 286, 492
HinfI GANTC 3 cut(s) 107, 356, 807
HpaII CCGG 1 cut(s) 119
HphI GGTGA 1 cut(s) 219
Hpy188I TCNGA 4 cut(s) 395, 477, 804, 927
Hpy188III TCNNGA 5 cut(s) 275, 499, 832, 850, 908
Hpy99I CGWCG 3 cut(s) 101, 104, 115
HpyAV CCTTC 5 cut(s) 293, 505, 637, 847, 884
HpyCH4III ACNGT 2 cut(s) 620, 668
HpyCH4V TGCA 8 cut(s) 16, 240, 262, 321, 330, 455, 470, 564
HpyF10VI GCNNNNNNNGC 5 cut(s) 195, 259, 327, 722, 794
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 4 cut(s) 234, 720, 881, 917
HspAI GCGC 2 cut(s) 286, 492
KasI GGCGCC 1 cut(s) 491
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 3 cut(s) 277, 523, 904
LguI GCTCTTC 1 cut(s) 912
LmnI GCTCC 3 cut(s) 131, 167, 272
Lsp1109I GCAGC 4 cut(s) 28, 576, 644, 772
LweI GCATC 1 cut(s) 190
MaeI CTAG 2 cut(s) 20, 54
MaeIII GTNAC 2 cut(s) 59, 884
MalI GATC 3 cut(s) 279, 525, 906
MboI GATC 3 cut(s) 277, 523, 904
MboII GAAGA 3 cut(s) 158, 338, 929
MfeI CAATTG 1 cut(s) 456
MflI RGATCY 1 cut(s) 904
MhlI GDGCHC 1 cut(s) 128
MluCI AATT 6 cut(s) 338, 389, 396, 447, 456, 502
Mly113I GGCGCC 1 cut(s) 492
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 1 cut(s) 882
MnlI CCTC 6 cut(s) 81, 175, 358, 706, 834, 862
MroXI GAANNNNTTC 1 cut(s) 506
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 235
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 4 cut(s) 92, 210, 728, 782
MunI CAATTG 1 cut(s) 456
MvaI CCWGG 4 cut(s) 92, 210, 728, 782
MwoI GCNNNNNNNGC 5 cut(s) 195, 259, 327, 722, 794
NarI GGCGCC 1 cut(s) 492
NdeI CATATG 1 cut(s) 361
NdeII GATC 3 cut(s) 277, 523, 904
NlaIII CATG 4 cut(s) 234, 720, 881, 917
NlaIV GGNNCC 5 cut(s) 45, 69, 163, 493, 906
NmuCI GTSAC 1 cut(s) 59
NspI RCATGY 1 cut(s) 720
PaeI GCATGC 1 cut(s) 720
PciSI GCTCTTC 1 cut(s) 912
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 2 cut(s) 356, 807
PkrI GCNGC 4 cut(s) 18, 591, 659, 787
PleI GAGTC 1 cut(s) 115
PluTI GGCGCC 1 cut(s) 495
PpsI GAGTC 1 cut(s) 115
PpuMI RGGWCCY 1 cut(s) 871
Psp124BI GAGCTC 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 871
Psp6I CCWGG 4 cut(s) 90, 208, 726, 780
PspGI CCWGG 4 cut(s) 90, 208, 726, 780
PspN4I GGNNCC 5 cut(s) 45, 69, 163, 493, 906
PspPI GGNCC 2 cut(s) 67, 871
PspPPI RGGWCCY 1 cut(s) 871
PsuI RGATCY 1 cut(s) 904
RsaI GTAC 2 cut(s) 438, 860
RsaNI GTAC 2 cut(s) 437, 859
RseI CAYNNNNRTG 1 cut(s) 235
SacI GAGCTC 1 cut(s) 128
SapI GCTCTTC 1 cut(s) 912
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 4 cut(s) 17, 590, 658, 786
Sau3AI GATC 3 cut(s) 277, 523, 904
Sau96I GGNCC 2 cut(s) 67, 871
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 4 cut(s) 92, 210, 728, 782
SduI GDGCHC 1 cut(s) 128
SetI ASST 6 cut(s) 21, 128, 153, 269, 772, 876
SfaNI GCATC 1 cut(s) 190
SfcI CTRYAG 1 cut(s) 77
SfoI GGCGCC 1 cut(s) 493
SinI GGWCC 2 cut(s) 67, 871
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 2 cut(s) 152, 935
SmoI CTYRAG 2 cut(s) 152, 935
SphI GCATGC 1 cut(s) 720
Sse9I AATT 6 cut(s) 338, 389, 396, 447, 456, 502
SsiI CCGC 1 cut(s) 35
SspDI GGCGCC 1 cut(s) 491
SspI AATATT 1 cut(s) 687
SspMI CTAG 2 cut(s) 20, 54
SstI GAGCTC 1 cut(s) 128
StyD4I CCNGG 4 cut(s) 90, 208, 726, 780
TaaI ACNGT 2 cut(s) 620, 668
TaqI TCGA 1 cut(s) 349
TasI AATT 6 cut(s) 338, 389, 396, 447, 456, 502
TatI WGTACW 2 cut(s) 436, 858
TfiI GAWTC 2 cut(s) 356, 807
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 5 cut(s) 136, 287, 323, 517, 623
TseFI GTSAC 1 cut(s) 59
TseI GCWGC 4 cut(s) 16, 589, 657, 785
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 6 cut(s) 17, 159, 332, 495, 866, 930
TspGWI ACGGA 1 cut(s) 661
TspRI CASTG 5 cut(s) 136, 287, 323, 517, 623
VpaK11BI GGWCC 2 cut(s) 67, 871
XapI RAATTY 3 cut(s) 389, 396, 502
XceI RCATGY 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 506
XspI CTAG 2 cut(s) 20, 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.