RchiOBHm_Chr2g0098951

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004026
Physical Location & Seq
Forward (+)
11275247 .. 11276012
766 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 534 bp
ATGGTCTTATATCTTGCAGCCAAATCTTGGTTTTTTTTTGGCAGTATCTCAAGCTTGCCAATGGGTTTTCTCAGTAAAGTCTCCGAGCTCCGTGCTAGGGCTCGGAGTCATAAAAAGCTCAAGAAGAACAAGCAGCTACAGACAGTGGAGATAAAAGTGAAGATGGACTGCGAAGGTTGCGAGAGAAGAGTGAAGAAATCAGTGGAAGGAATGAAAGGAGTCACGAGTGTTGAAGTGGAACCTAAGCAAAGCAAGCTCACTGTTATTGGCTATGTGGACCCCAACAAGGTGTTGCATCGTGTCAGGCATCGAACGGGGAAGAAGGCGGATCTGTGGCCGTATGTACCATACGACGTCGTCGACCATTCATGGGCTCCCGGGGCTTACGATAGGAAGGCACCACCTGGGTACGTGAGGAACGTGCTGAATGACCCTGACCCTCAGGCTTCTGCTCTCGCACGCGCCAACTCCACCGAGGTGAAGTATACGACGGCGTTTAGTGACGAAAATCCAAATGCTTGTGTGGTTATGTAG

Protein Analysis

177

Amino Acids

19.91

Weight (kDa)

9.68

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 52 - 107 6.6e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
AccB1I GGYRCC 1 cut(s) 397
AccB7I CCANNNNNTGG 1 cut(s) 27
AccI GTMKAC 2 cut(s) 360, 485
AccII CGCG 1 cut(s) 462
AciI CCGC 1 cut(s) 326
AclWI GGATC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 335
AcyI GRCGYC 1 cut(s) 354
AfaI GTAC 2 cut(s) 345, 410
AfiI CCNNNNNNNGG 4 cut(s) 27, 97, 286, 370
AgsI TTSAA 1 cut(s) 233
AjnI CCWGG 1 cut(s) 403
AleI CACNNNNGTG 1 cut(s) 476
AluBI AGCT 5 cut(s) 54, 88, 118, 136, 256
AluI AGCT 5 cut(s) 54, 88, 118, 136, 256
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 1 cut(s) 85
AlwI GGATC 1 cut(s) 336
Ama87I CYCGRG 1 cut(s) 377
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 2 cut(s) 17, 133
AspLEI GCGC 1 cut(s) 464
AspS9I GGNCC 1 cut(s) 277
AsuC2I CCSGG 2 cut(s) 378, 379
AsuHPI GGTGA 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 1 cut(s) 277
AxyI CCTNAGG 1 cut(s) 441
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 3 cut(s) 90, 103, 376
BauI CACGAG 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 2 cut(s) 29, 145
BccI CCATC 1 cut(s) 157
BceAI ACGGC 2 cut(s) 322, 507
BcgI CGANNNNNNTGC 2 cut(s) 74, 108
BciT130I CCWGG 1 cut(s) 405
BcnI CCSGG 2 cut(s) 378, 379
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 1 cut(s) 96
BfmI CTRYAG 1 cut(s) 137
BisI GCNGC 2 cut(s) 18, 134
BlsI GCNGC 2 cut(s) 19, 135
Bme1390I CCNGG 3 cut(s) 378, 379, 405
Bme18I GGWCC 1 cut(s) 277
BmeT110I CYCGRG 1 cut(s) 377
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 4 cut(s) 240, 279, 375, 399
BmrFI CCNGG 3 cut(s) 378, 379, 405
BmsI GCATC 2 cut(s) 304, 316
Bpu10I CCTNAGC 1 cut(s) 243
BpuEI CTTGAG 2 cut(s) 34, 104
BpuMI CCSGG 2 cut(s) 378, 379
BsaAI YACGTR 1 cut(s) 412
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 4 cut(s) 377, 378, 404, 474
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 97, 286, 370
Bse21I CCTNAGG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 405
BseDI CCNNGG 4 cut(s) 377, 378, 404, 474
BseLI CCNNNNNNNGG 4 cut(s) 27, 97, 286, 370
BseMII CTCAG 2 cut(s) 85, 455
BseXI GCAGC 2 cut(s) 29, 145
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 1 cut(s) 337
BshNI GGYRCC 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 90
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 1 cut(s) 378
BslI CCNNNNNNNGG 4 cut(s) 27, 97, 286, 370
BsmAI GTCTC 1 cut(s) 85
BsnI GGCC 1 cut(s) 337
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 90, 103, 376
Bsp143I GATC 1 cut(s) 328
BspACI CCGC 1 cut(s) 326
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 2 cut(s) 84, 454
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 4 cut(s) 240, 279, 375, 399
BspPI GGATC 1 cut(s) 336
BspT107I GGYRCC 1 cut(s) 397
BssECI CCNNGG 4 cut(s) 377, 378, 404, 474
BssMI GATC 1 cut(s) 328
BssNAI GTATAC 1 cut(s) 486
BssNI GRCGYC 1 cut(s) 354
BssSI CACGAG 1 cut(s) 223
Bst1107I GTATAC 1 cut(s) 486
Bst2BI CACGAG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 2 cut(s) 145, 262
Bst6I CTCTTC 1 cut(s) 181
BstACI GRCGYC 1 cut(s) 354
BstBAI YACGTR 1 cut(s) 412
BstC8I GCNNGC 3 cut(s) 56, 254, 460
BstDEI CTNAG 3 cut(s) 71, 243, 441
BstFNI CGCG 1 cut(s) 462
BstHHI GCGC 1 cut(s) 464
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 1 cut(s) 328
BstMWI GCNNNNNNNGC 3 cut(s) 177, 253, 380
BstNI CCWGG 1 cut(s) 405
BstSCI CCNGG 3 cut(s) 376, 377, 403
BstSFI CTRYAG 1 cut(s) 137
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 2 cut(s) 29, 145
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BstZ17I GTATAC 1 cut(s) 486
Bsu36I CCTNAGG 1 cut(s) 441
BsuRI GGCC 1 cut(s) 337
BtsIMutI CAGTG 3 cut(s) 150, 207, 258
Cac8I GCNNGC 3 cut(s) 56, 254, 460
CfoI GCGC 1 cut(s) 464
Cfr13I GGNCC 1 cut(s) 277
Cfr9I CCCGGG 1 cut(s) 377
Csp6I GTAC 2 cut(s) 344, 409
CviAII CATG 1 cut(s) 369
CviQI GTAC 2 cut(s) 344, 409
DdeI CTNAG 3 cut(s) 71, 243, 441
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 181
EarI CTCTTC 1 cut(s) 181
EciI GGCGGA 1 cut(s) 341
Ecl136II GAGCTC 1 cut(s) 88
Eco24I GRGCYC 3 cut(s) 90, 103, 376
Eco47I GGWCC 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 88
Eco81I CCTNAGG 1 cut(s) 441
Eco88I CYCGRG 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 403
EcoT38I GRGCYC 3 cut(s) 90, 103, 376
FaeI CATG 1 cut(s) 372
FaiI YATR 8 cut(s) 10, 111, 273, 342, 349, 370, 486, 530
FatI CATG 1 cut(s) 368
FblI GTMKAC 2 cut(s) 360, 485
Fnu4HI GCNGC 2 cut(s) 18, 134
FriOI GRGCYC 3 cut(s) 90, 103, 376
Fsp4HI GCNGC 2 cut(s) 18, 134
FspBI CTAG 1 cut(s) 96
GlaI GCGC 1 cut(s) 463
GluI GCNGC 2 cut(s) 18, 134
HaeIII GGCC 1 cut(s) 337
HapII CCGG 1 cut(s) 378
HhaI GCGC 1 cut(s) 464
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 1 cut(s) 372
Hin6I GCGC 1 cut(s) 462
HinP1I GCGC 1 cut(s) 462
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 2 cut(s) 106, 219
HpaII CCGG 1 cut(s) 378
HphI GGTGA 1 cut(s) 490
Hpy166II GTNNAC 3 cut(s) 277, 361, 486
Hpy188I TCNGA 2 cut(s) 85, 105
Hpy188III TCNNGA 2 cut(s) 121, 223
Hpy8I GTNNAC 3 cut(s) 277, 361, 486
Hpy99I CGWCG 4 cut(s) 356, 359, 362, 493
HpyAV CCTTC 4 cut(s) 167, 200, 316, 388
HpyCH4III ACNGT 2 cut(s) 145, 262
HpyCH4IV ACGT 3 cut(s) 354, 411, 420
HpyCH4V TGCA 2 cut(s) 17, 295
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 253, 380
HpyF3I CTNAG 3 cut(s) 71, 243, 441
HpySE526I ACGT 3 cut(s) 354, 411, 420
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 1 cut(s) 372
HspAI GCGC 1 cut(s) 462
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 2 cut(s) 93, 379
LpnPI CCDG 6 cut(s) 289, 390, 391, 417, 428, 447
Lsp1109I GCAGC 2 cut(s) 29, 145
LweI GCATC 2 cut(s) 304, 316
MaeI CTAG 1 cut(s) 96
MaeII ACGT 3 cut(s) 354, 411, 420
MaeIII GTNAC 2 cut(s) 220, 500
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 5 cut(s) 136, 172, 198, 205, 331
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 3 cut(s) 90, 103, 376
MlyI GAGTC 2 cut(s) 115, 228
MnlI CCTC 3 cut(s) 408, 450, 469
MslI CAYNNNNRTG 1 cut(s) 476
MspI CCGG 1 cut(s) 378
MspR9I CCNGG 3 cut(s) 378, 379, 405
MvaI CCWGG 1 cut(s) 405
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 3 cut(s) 177, 253, 380
NciI CCSGG 2 cut(s) 378, 379
NdeII GATC 1 cut(s) 328
NlaIII CATG 1 cut(s) 372
NlaIV GGNNCC 4 cut(s) 240, 279, 375, 399
NmuCI GTSAC 2 cut(s) 220, 500
OliI CACNNNNGTG 1 cut(s) 476
PcsI WCGNNNNNNNCGW 2 cut(s) 357, 417
PflFI GACNNNGTC 1 cut(s) 356
PflMI CCANNNNNTGG 1 cut(s) 27
PkrI GCNGC 2 cut(s) 19, 135
PleI GAGTC 2 cut(s) 114, 227
PpsI GAGTC 2 cut(s) 114, 227
Ppu21I YACGTR 1 cut(s) 412
Psp124BI GAGCTC 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 4 cut(s) 240, 279, 375, 399
PspPI GGNCC 1 cut(s) 277
PsuI RGATCY 1 cut(s) 328
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 2 cut(s) 345, 410
RsaNI GTAC 2 cut(s) 344, 409
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 90
SalI GTCGAC 1 cut(s) 359
SatI GCNGC 2 cut(s) 18, 134
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 1 cut(s) 277
SchI GAGTC 2 cut(s) 115, 228
ScrFI CCNGG 3 cut(s) 378, 379, 405
SduI GDGCHC 3 cut(s) 90, 103, 376
SfaNI GCATC 2 cut(s) 304, 316
SfcI CTRYAG 1 cut(s) 137
SinI GGWCC 1 cut(s) 277
SmaI CCCGGG 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 2 cut(s) 49, 119
SmoI CTYRAG 2 cut(s) 49, 119
SsiI CCGC 1 cut(s) 326
SspMI CTAG 1 cut(s) 96
SstI GAGCTC 1 cut(s) 90
StyD4I CCNGG 3 cut(s) 376, 377, 403
TaaI ACNGT 2 cut(s) 145, 262
TaiI ACGT 3 cut(s) 357, 414, 423
TaqI TCGA 2 cut(s) 310, 360
TscAI CASTG 3 cut(s) 150, 207, 265
TseFI GTSAC 2 cut(s) 220, 500
TseI GCWGC 2 cut(s) 17, 133
Tsp45I GTSAC 2 cut(s) 220, 500
TspDTI ATGAA 2 cut(s) 227, 357
TspGWI ACGGA 1 cut(s) 80
TspMI CCCGGG 1 cut(s) 377
TspRI CASTG 3 cut(s) 150, 207, 265
Tth111I GACNNNGTC 1 cut(s) 356
Van91I CCANNNNNTGG 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 277
XmaI CCCGGG 1 cut(s) 377
XmiI GTMKAC 2 cut(s) 360, 485
XspI CTAG 1 cut(s) 96
ZraI GACGTC 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.