RchiOBHm_Chr2g0113201

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011604
Physical Location & Seq
Forward (+)
25386967 .. 25391112
4146 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGGATAAAAAGGAAAGAGAGAAGCCAAAGAAGGCATCGGATTATTTAAGTCTCTTAAAAGACTCATTTACTTGTGGCATCTGCAATTGGCTTATTAGAGGAAGGATTTTATTGGACAGATTAGATCTTGATGATCCAATAGAGAAAAAGGACAAAGCATCATTATCGGAGCAGCTGAAGTCTATGCCTAAGATTTCTGTTGATTGTGTGGTTTCTAATCACGATTGCATATGCCACAGGGTGGGCTGTTGGCAATGCATGCCATTGGACGTCCTAAAGTCCCGTTCTGTTCAAGAGTTGGTGGATTTGAAGTATCAATTCTTTTGCAGGCGTCTTGCTTGCCTTTTCCTTTCCTTTGTAAATATATTGAACCTTTTCTTCACGTTTTCAGGAAATCATGCTAAAGTTTCTGAAACGGCTGATATTTTTGGGAGAAGCCTTCATTTCTTATTCAACAGAGCTTATACCTCTAACGGTGACTGGACTATTTATAATTCATTATTGACACGGCAGGTCCAAGGGACTGCCTTGGCAGTTGAGCAAGCAGAGCTTCTATACTACGCAACCTTTATGAGAATCATACCAGTTATGTTATTGATAGAGAAGGAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

204

Amino Acids

23.58

Weight (kDa)

8.34

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
AatII GACGTC 1 cut(s) 273
Acc36I ACCTGC 1 cut(s) 502
AccB7I CCANNNNNTGG 1 cut(s) 241
AclWI GGATC 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 197
AcyI GRCGYC 2 cut(s) 270, 331
AdeI CACNNNGTG 1 cut(s) 241
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 4 cut(s) 293, 310, 370, 454
AluBI AGCT 3 cut(s) 175, 461, 550
AluI AGCT 3 cut(s) 175, 461, 550
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 1 cut(s) 128
ApeKI GCWGC 1 cut(s) 172
Asp700I GAANNNNTTC 1 cut(s) 374
AspS9I GGNCC 1 cut(s) 514
AsuHPI GGTGA 1 cut(s) 488
AvaII GGWCC 1 cut(s) 514
BbvI GCAGC 1 cut(s) 184
BceAI ACGGC 2 cut(s) 432, 524
BcgI CGANNNNNNTGC 2 cut(s) 147, 181
BcoDI GTCTC 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 502
BglII AGATCT 1 cut(s) 124
BisI GCNGC 1 cut(s) 173
BlsI GCNGC 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 514
BmgT120I GGNCC 1 cut(s) 514
BmsI GCATC 3 cut(s) 44, 87, 167
BsaHI GRCGYC 2 cut(s) 270, 331
BsaJI CCNNGG 2 cut(s) 517, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse1I ACTGG 2 cut(s) 485, 584
Bse3DI GCAATG 1 cut(s) 260
BseDI CCNNGG 2 cut(s) 517, 528
BseLI CCNNNNNNNGG 1 cut(s) 241
BseMI GCAATG 1 cut(s) 260
BseNI ACTGG 2 cut(s) 485, 584
BseXI GCAGC 1 cut(s) 184
BslFI GGGAC 2 cut(s) 265, 535
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 1 cut(s) 56
BsmFI GGGAC 2 cut(s) 265, 535
Bsp143I GATC 2 cut(s) 124, 133
BspMI ACCTGC 1 cut(s) 502
BspPI GGATC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 260
BsrI ACTGG 2 cut(s) 485, 584
BssECI CCNNGG 2 cut(s) 517, 528
BssMI GATC 2 cut(s) 124, 133
BssNI GRCGYC 2 cut(s) 270, 331
BssT1I CCWWGG 2 cut(s) 517, 528
Bst4CI ACNGT 1 cut(s) 476
BstACI GRCGYC 2 cut(s) 270, 331
BstAPI GCANNNNNTGC 1 cut(s) 259
BstC8I GCNNGC 4 cut(s) 260, 329, 340, 543
BstDEI CTNAG 1 cut(s) 189
BstKTI GATC 2 cut(s) 127, 136
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 2 cut(s) 124, 133
BstMWI GCNNNNNNNGC 2 cut(s) 259, 547
BstNSI RCATGY 1 cut(s) 262
BstV1I GCAGC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BveI ACCTGC 1 cut(s) 502
Cac8I GCNNGC 4 cut(s) 260, 329, 340, 543
Cfr13I GGNCC 1 cut(s) 514
CseI GACGC 1 cut(s) 320
CviAII CATG 2 cut(s) 259, 398
CviJI RGCY 8 cut(s) 25, 91, 175, 246, 419, 438, 461, 550
CviKI_1 RGCY 8 cut(s) 25, 91, 175, 246, 419, 438, 461, 550
DdeI CTNAG 1 cut(s) 189
DpnI GATC 2 cut(s) 126, 135
DpnII GATC 2 cut(s) 124, 133
DraIII CACNNNGTG 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 517, 528
Eco47I GGWCC 1 cut(s) 514
Eco57I CTGAAG 1 cut(s) 197
EcoT14I CCWWGG 2 cut(s) 517, 528
EcoT22I ATGCAT 1 cut(s) 260
ErhI CCWWGG 2 cut(s) 517, 528
FaeI CATG 2 cut(s) 262, 401
FalI AAGNNNNNCTT 2 cut(s) 534, 566
FaqI GGGAC 2 cut(s) 265, 535
FatI CATG 2 cut(s) 258, 397
FauNDI CATATG 1 cut(s) 230
Fnu4HI GCNGC 1 cut(s) 173
Fsp4HI GCNGC 1 cut(s) 173
GluI GCNGC 1 cut(s) 173
HgaI GACGC 1 cut(s) 320
Hin1I GRCGYC 2 cut(s) 270, 331
Hin1II CATG 2 cut(s) 262, 401
HinfI GANTC 2 cut(s) 62, 576
HphI GGTGA 1 cut(s) 488
Hpy188I TCNGA 3 cut(s) 40, 169, 412
Hpy188III TCNNGA 4 cut(s) 128, 221, 293, 390
HpyAV CCTTC 4 cut(s) 25, 96, 449, 598
HpyCH4III ACNGT 1 cut(s) 476
HpyCH4IV ACGT 2 cut(s) 270, 383
HpyCH4V TGCA 4 cut(s) 84, 228, 258, 327
HpyF10VI GCNNNNNNNGC 2 cut(s) 259, 547
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 2 cut(s) 270, 383
Hsp92I GRCGYC 2 cut(s) 270, 331
Hsp92II CATG 2 cut(s) 262, 401
Kzo9I GATC 2 cut(s) 124, 133
LmnI GCTCC 1 cut(s) 169
LpnPI CCDG 6 cut(s) 223, 313, 375, 466, 497, 597
Lsp1109I GCAGC 1 cut(s) 184
LweI GCATC 3 cut(s) 44, 87, 167
MaeII ACGT 2 cut(s) 270, 383
MaeIII GTNAC 1 cut(s) 476
MalI GATC 2 cut(s) 126, 135
MboI GATC 2 cut(s) 124, 133
MboII GAAGA 1 cut(s) 370
MfeI CAATTG 1 cut(s) 85
MflI RGATCY 1 cut(s) 124
MluCI AATT 3 cut(s) 85, 317, 493
MlyI GAGTC 1 cut(s) 56
MnlI CCTC 2 cut(s) 92, 478
Mph1103I ATGCAT 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 374
MseI TTAA 2 cut(s) 47, 56
MspA1I CMGCKG 1 cut(s) 175
MunI CAATTG 1 cut(s) 85
MwoI GCNNNNNNNGC 2 cut(s) 259, 547
NdeI CATATG 1 cut(s) 230
NdeII GATC 2 cut(s) 124, 133
NlaIII CATG 2 cut(s) 262, 401
NmuCI GTSAC 1 cut(s) 476
NsiI ATGCAT 1 cut(s) 260
NspI RCATGY 1 cut(s) 262
PaeI GCATGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 1 cut(s) 576
PflMI CCANNNNNTGG 1 cut(s) 241
PkrI GCNGC 1 cut(s) 174
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
PsiI TTATAA 1 cut(s) 492
PspPI GGNCC 1 cut(s) 514
PsuI RGATCY 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 175
SaqAI TTAA 2 cut(s) 47, 56
SatI GCNGC 1 cut(s) 173
Sau3AI GATC 2 cut(s) 124, 133
Sau96I GGNCC 1 cut(s) 514
SchI GAGTC 1 cut(s) 56
SetI ASST 9 cut(s) 177, 273, 375, 386, 463, 470, 516, 552, 569
SfaNI GCATC 3 cut(s) 44, 87, 167
SinI GGWCC 1 cut(s) 514
SphI GCATGC 1 cut(s) 262
Sse9I AATT 3 cut(s) 85, 317, 493
StyI CCWWGG 2 cut(s) 517, 528
TaaI ACNGT 1 cut(s) 476
TaiI ACGT 2 cut(s) 273, 386
TasI AATT 3 cut(s) 85, 317, 493
TfiI GAWTC 1 cut(s) 576
Tru1I TTAA 2 cut(s) 47, 56
Tru9I TTAA 2 cut(s) 47, 56
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 2 cut(s) 431, 486
Van91I CCANNNNNTGG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 514
XceI RCATGY 1 cut(s) 262
XmnI GAANNNNTTC 1 cut(s) 374
ZraI GACGTC 1 cut(s) 271
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.