RLG00000032863

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011604
Physical Location & Seq
Reverse (-)
20135715 .. 20137215
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032863

Sequence Viewer

Length: 798 bp
ATGGTGCCGATCCTCTTCTTGAACTGGTTCTCGCTCAGGTATGATGCATACACAGTTGAATCAGTTCTTGCTCAGGCTCTAACCTATGCTTTGAAGGCCATAGAAACTCGAAAAGAATTATACGATAAGTATTTTGCTGCTAAAGATAGGAATATATATAAGAAGAGTAAGAAGAAGATGGATAAAAAGGAAAGAGAGAAGCCAAAGAAGGCATCGGATTATTTAAGTCTCTTAAAAGACTCATTTACTTATGGCATCTGCAATTGGCTTATTAGAGGAAGGATTTTATTGGACAGATTAGATCTTGATGATCCAATAGAGAAAAAGGACAAAGCATCATTATCAGAGCAGCTGAAGTCTATGCCTAAGATTTCTGTTGATTGTGTGGTTTCTAATCACGGGTGCATATGCCACAGGGTGGGCTGTTGGCAATGCATGCCATTGGATGTCCGAAAGTCCCGTTCTGTTCAAGAGTTAGTGGATTTGAAGTATCAATTCTTTTGCAGGCGTCTTGCTTGCCTTTTCCTTACCTTTGTAAATATATTGAACCTTTTCTTCACGTTTTCAGGAAATCATGCTGAAGTTTCTGAAACGGCTGATATTTTTGGGAGAAGCCTTCATTTCTTATTCAACAGAGCTTATACCTCCAACGGTGACTGGACTATTTATAATTCATTATTGACACGGCAGGTCCAAGGGACTGCCTTGGCAGTTGAGCAAGCAGAGCTTCTATACTACGCAACCTTTGTGAGAATCATACCAGGTGTGTGTTCACAATTATATTTACATTGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

266

Amino Acids

30.8

Weight (kDa)

9.06

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
Acc36I ACCTGC 1 cut(s) 679
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 418
AclWI GGATC 2 cut(s) 4, 305
AcuI CTGAAG 2 cut(s) 374, 600
AcyI GRCGYC 1 cut(s) 508
AdeI CACNNNGTG 1 cut(s) 418
AfiI CCNNNNNNNGG 1 cut(s) 418
AgsI TTSAA 7 cut(s) 22, 59, 94, 470, 487, 547, 631
AjnI CCWGG 1 cut(s) 760
AluBI AGCT 3 cut(s) 352, 638, 727
AluI AGCT 3 cut(s) 352, 638, 727
Alw26I GTCTC 1 cut(s) 233
AlwI GGATC 2 cut(s) 4, 305
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 2 cut(s) 137, 349
Asp700I GAANNNNTTC 3 cut(s) 26, 63, 551
AspS9I GGNCC 1 cut(s) 691
AsuHPI GGTGA 1 cut(s) 665
AvaII GGWCC 1 cut(s) 691
BanI GGYRCC 1 cut(s) 4
BbvI GCAGC 2 cut(s) 124, 361
BccI CCATC 1 cut(s) 172
BceAI ACGGC 2 cut(s) 609, 701
BciT130I CCWGG 1 cut(s) 762
BcoDI GTCTC 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 679
BglII AGATCT 1 cut(s) 301
BisI GCNGC 2 cut(s) 138, 350
BlsI GCNGC 2 cut(s) 139, 351
Bme1390I CCNGG 1 cut(s) 762
Bme18I GGWCC 1 cut(s) 691
BmgT120I GGNCC 1 cut(s) 691
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 762
BmsI GCATC 4 cut(s) 34, 221, 264, 344
Bpu10I CCTNAGC 2 cut(s) 35, 72
BsaHI GRCGYC 1 cut(s) 508
BsaJI CCNNGG 2 cut(s) 694, 705
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse1I ACTGG 2 cut(s) 29, 662
Bse3DI GCAATG 1 cut(s) 437
BseBI CCWGG 1 cut(s) 762
BseDI CCNNGG 2 cut(s) 694, 705
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMI GCAATG 1 cut(s) 437
BseMII CTCAG 2 cut(s) 49, 86
BseNI ACTGG 2 cut(s) 29, 662
BseXI GCAGC 2 cut(s) 124, 361
BshFI GGCC 1 cut(s) 98
BshNI GGYRCC 1 cut(s) 4
BslFI GGGAC 2 cut(s) 442, 712
BslI CCNNNNNNNGG 1 cut(s) 418
BsmAI GTCTC 1 cut(s) 233
BsmFI GGGAC 2 cut(s) 442, 712
BsnI GGCC 1 cut(s) 98
Bsp143I GATC 3 cut(s) 9, 301, 310
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 2 cut(s) 48, 85
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 679
BspPI GGATC 2 cut(s) 4, 305
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 437
BsrI ACTGG 2 cut(s) 29, 662
BssECI CCNNGG 2 cut(s) 694, 705
BssMI GATC 3 cut(s) 9, 301, 310
BssNI GRCGYC 1 cut(s) 508
BssT1I CCWWGG 2 cut(s) 694, 705
Bst2UI CCWGG 1 cut(s) 762
Bst4CI ACNGT 2 cut(s) 55, 653
Bst6I CTCTTC 2 cut(s) 20, 158
BstACI GRCGYC 1 cut(s) 508
BstAPI GCANNNNNTGC 1 cut(s) 436
BstC8I GCNNGC 4 cut(s) 437, 506, 517, 720
BstDEI CTNAG 3 cut(s) 35, 72, 366
BstF5I GGATG 1 cut(s) 451
BstKTI GATC 3 cut(s) 12, 304, 313
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 3 cut(s) 9, 301, 310
BstMWI GCNNNNNNNGC 3 cut(s) 95, 436, 724
BstNI CCWGG 1 cut(s) 762
BstNSI RCATGY 1 cut(s) 439
BstSCI CCNGG 1 cut(s) 760
BstV1I GCAGC 2 cut(s) 124, 361
BstX2I RGATCY 1 cut(s) 301
BstYI RGATCY 1 cut(s) 301
BsuRI GGCC 1 cut(s) 98
BtsCI GGATG 1 cut(s) 451
BveI ACCTGC 1 cut(s) 679
Cac8I GCNNGC 4 cut(s) 437, 506, 517, 720
Cfr13I GGNCC 1 cut(s) 691
CseI GACGC 1 cut(s) 497
CsiI ACCWGGT 1 cut(s) 760
CviAII CATG 2 cut(s) 436, 575
DdeI CTNAG 3 cut(s) 35, 72, 366
DpnI GATC 3 cut(s) 11, 303, 312
DpnII GATC 3 cut(s) 9, 301, 310
DraIII CACNNNGTG 1 cut(s) 418
Eam1104I CTCTTC 2 cut(s) 20, 158
EarI CTCTTC 2 cut(s) 20, 158
Eco130I CCWWGG 2 cut(s) 694, 705
Eco47I GGWCC 1 cut(s) 691
Eco57I CTGAAG 2 cut(s) 374, 600
EcoRII CCWGG 1 cut(s) 760
EcoT14I CCWWGG 2 cut(s) 694, 705
EcoT22I ATGCAT 2 cut(s) 49, 437
ErhI CCWWGG 2 cut(s) 694, 705
FaeI CATG 2 cut(s) 439, 578
FalI AAGNNNNNCTT 2 cut(s) 711, 743
FaqI GGGAC 2 cut(s) 442, 712
FatI CATG 2 cut(s) 435, 574
FauNDI CATATG 1 cut(s) 407
Fnu4HI GCNGC 2 cut(s) 138, 350
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 2 cut(s) 138, 350
GluI GCNGC 2 cut(s) 138, 350
HaeIII GGCC 1 cut(s) 98
HgaI GACGC 1 cut(s) 497
Hin1I GRCGYC 1 cut(s) 508
Hin1II CATG 2 cut(s) 439, 578
HinfI GANTC 3 cut(s) 59, 239, 753
HphI GGTGA 1 cut(s) 665
Hpy166II GTNNAC 1 cut(s) 773
Hpy188I TCNGA 4 cut(s) 217, 346, 452, 589
Hpy188III TCNNGA 4 cut(s) 19, 305, 470, 567
Hpy8I GTNNAC 1 cut(s) 773
HpyAV CCTTC 4 cut(s) 88, 202, 273, 626
HpyCH4III ACNGT 2 cut(s) 55, 653
HpyCH4IV ACGT 1 cut(s) 560
HpyCH4V TGCA 5 cut(s) 47, 261, 405, 435, 504
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 436, 724
HpyF3I CTNAG 3 cut(s) 35, 72, 366
HpySE526I ACGT 1 cut(s) 560
Hsp92I GRCGYC 1 cut(s) 508
Hsp92II CATG 2 cut(s) 439, 578
Kzo9I GATC 3 cut(s) 9, 301, 310
Lsp1109I GCAGC 2 cut(s) 124, 361
LweI GCATC 4 cut(s) 34, 221, 264, 344
MabI ACCWGGT 1 cut(s) 760
MaeII ACGT 1 cut(s) 560
MaeIII GTNAC 2 cut(s) 653, 791
MalI GATC 3 cut(s) 11, 303, 312
MboI GATC 3 cut(s) 9, 301, 310
MboII GAAGA 5 cut(s) 7, 175, 184, 187, 547
MfeI CAATTG 1 cut(s) 262
MflI RGATCY 1 cut(s) 301
MluCI AATT 5 cut(s) 116, 262, 494, 670, 776
MlyI GAGTC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 672
MnlI CCTC 3 cut(s) 23, 269, 655
Mph1103I ATGCAT 2 cut(s) 49, 437
MroXI GAANNNNTTC 3 cut(s) 26, 63, 551
MseI TTAA 2 cut(s) 224, 233
MspA1I CMGCKG 1 cut(s) 352
MspR9I CCNGG 1 cut(s) 762
MunI CAATTG 1 cut(s) 262
MvaI CCWGG 1 cut(s) 762
MwoI GCNNNNNNNGC 3 cut(s) 95, 436, 724
NdeI CATATG 1 cut(s) 407
NdeII GATC 3 cut(s) 9, 301, 310
NlaIII CATG 2 cut(s) 439, 578
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 653
NsiI ATGCAT 2 cut(s) 49, 437
NspI RCATGY 1 cut(s) 439
PaeI GCATGC 1 cut(s) 439
PdmI GAANNNNTTC 3 cut(s) 26, 63, 551
PfeI GAWTC 2 cut(s) 59, 753
PflMI CCANNNNNTGG 1 cut(s) 418
PkrI GCNGC 2 cut(s) 139, 351
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PsiI TTATAA 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 760
PspGI CCWGG 1 cut(s) 760
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 691
PsuI RGATCY 1 cut(s) 301
PvuII CAGCTG 1 cut(s) 352
SaqAI TTAA 2 cut(s) 224, 233
SatI GCNGC 2 cut(s) 138, 350
Sau3AI GATC 3 cut(s) 9, 301, 310
Sau96I GGNCC 1 cut(s) 691
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 762
SexAI ACCWGGT 1 cut(s) 760
SfaNI GCATC 4 cut(s) 34, 221, 264, 344
SinI GGWCC 1 cut(s) 691
SphI GCATGC 1 cut(s) 439
Sse9I AATT 5 cut(s) 116, 262, 494, 670, 776
StyD4I CCNGG 1 cut(s) 760
StyI CCWWGG 2 cut(s) 694, 705
TaaI ACNGT 2 cut(s) 55, 653
TaiI ACGT 1 cut(s) 563
TaqI TCGA 1 cut(s) 109
TasI AATT 5 cut(s) 116, 262, 494, 670, 776
TfiI GAWTC 2 cut(s) 59, 753
Tru1I TTAA 2 cut(s) 224, 233
Tru9I TTAA 2 cut(s) 224, 233
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 2 cut(s) 137, 349
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 2 cut(s) 608, 663
Van91I CCANNNNNTGG 1 cut(s) 418
VpaK11BI GGWCC 1 cut(s) 691
XceI RCATGY 1 cut(s) 439
XmnI GAANNNNTTC 3 cut(s) 26, 63, 551
Zsp2I ATGCAT 2 cut(s) 49, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.