Rorug01G0041600

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0011604
Physical Location & Seq
Forward (+)
6817306 .. 6821036
3731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0041600.1

Sequence Viewer

Length: 1122 bp
ATGTGGACTGGAGGTAGCGTTTCTTTGGCTCTTTGGTATAAGGATGGAAATACATCTCGGGTAGATGGTGATGATGTTGACTGGGATACTGATGATGAGCTTGAGATTGAAAACTTCACTTTATCTTCTTCAGCTAATCCAGGCTCTGGGGAGGCTAGCTCATCATCAGGACCTTCCCAAACCAAAGTTTCTGATCATTTTGTCAGCATGGGATTTTCTCATAATATGGTTGCCAAAGCCATCCAAGAACATGGACTTGAAGATGCAGATTCAATTCTTGAAACCCTCCTCTTATATAACGCTCTTGAAAGTAGTCCTCCGGAGCAACATTCTCTGCCAGAGCTGCATTCCCCTCCAGAACAGTGCTCTCCTCAAGAACAGCAACAAATTGACTCGGATCTCTTTTCTTCAGATTATGATGGGAGCTTTCTAGATAGCTTTTCAGATCTCGATAGCTCTGATGATGAGGTAACCATCGATCCTACATCTGAGGCGGAGACAAAACTTATGTCTTTGGTTAAAATGGGGTACAATAAATTGGAGGCTTCAATGGCAATAGAAAGATGCGGTATTGACTCGTCACTTGTTGATTTGACTGATTTCATATCAGCTGCTCAAATTTCAAAGGCAGAAGATGGCCATCTTCCCCTTGAAGAGATGCGTACACATCTGCGGAGTAAGTATCCTGAGAAGAATAAAAGGGAACTTTGTGAATTTTCATTGCTGAAAAGGAAAAGGATTATCGGACATGGAAACCAGATCATAGGGGAAGATGCTGGTACAATTCATCTCCCGAATCCAATGAATGGGTTTGGGACTCCAGCTGATCTCTGTCAAATCCACAGAAGTCTTCCAGAGGCAGCCACAAAACCTCCTTATTTTTACTATGAGAATGTGGCATTGACTCCTAAAGGAGTTTGGAGCACAATATCCCGCTTTTTGTATGACGTGAAGCCTGAGTTTGTTGATTCGAAGTATTTTTGTGCTGCTGCAAGAAAAAGGGGTTATGTTCACAATCTTTCAATCAAAAACAGAATTCCTCTCGTTCCCCTTCCACCACAAACTATATTTGATGCATTTCAAGGAGGTGGTGGCCTACTTGGGATGATCGGACAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

373

Amino Acids

41.08

Weight (kDa)

4.65

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 146, 806
AccIII TCCGGA 1 cut(s) 319
AciI CCGC 4 cut(s) 494, 567, 673, 934
AclWI GGATC 2 cut(s) 405, 473
AcoI YGGCCR 1 cut(s) 637
AcsI RAATTY 3 cut(s) 618, 713, 1035
AcuI CTGAAG 2 cut(s) 114, 393
AfaI GTAC 3 cut(s) 530, 664, 781
AfiI CCNNNNNNNGG 2 cut(s) 146, 806
AjiI CACGTC 1 cut(s) 949
AjnI CCWGG 1 cut(s) 139
Alw21I GWGCWC 2 cut(s) 368, 926
Alw26I GTCTC 1 cut(s) 491
AlwI GGATC 2 cut(s) 405, 473
AlwNI CAGNNNCTG 1 cut(s) 146
Ama87I CYCGRG 1 cut(s) 57
Aor13HI TCCGGA 1 cut(s) 319
AoxI GGCC 2 cut(s) 637, 1093
ApeKI GCWGC 5 cut(s) 343, 611, 860, 986, 989
ApoI RAATTY 3 cut(s) 618, 713, 1035
ArsI GACNNNNNNTTYG 2 cut(s) 494, 526
AspS9I GGNCC 1 cut(s) 170
AsuHPI GGTGA 1 cut(s) 80
AsuII TTCGAA 1 cut(s) 971
AsuNHI GCTAGC 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 1 cut(s) 170
BalI TGGCCA 1 cut(s) 639
BbsI GAAGAC 1 cut(s) 842
Bbv12I GWGCWC 2 cut(s) 368, 926
BbvI GCAGC 5 cut(s) 330, 598, 872, 973, 976
BccI CCATC 7 cut(s) 38, 59, 248, 413, 482, 629, 648
BciT130I CCWGG 1 cut(s) 141
BciVI GTATCC 2 cut(s) 79, 693
BclI TGATCA 1 cut(s) 193
BcoDI GTCTC 1 cut(s) 491
BfaI CTAG 2 cut(s) 156, 431
BfuI GTATCC 2 cut(s) 79, 693
BglII AGATCT 1 cut(s) 445
BisI GCNGC 5 cut(s) 344, 612, 861, 987, 990
BlsI GCNGC 5 cut(s) 345, 613, 862, 988, 991
Bme1390I CCNGG 1 cut(s) 141
Bme18I GGWCC 1 cut(s) 170
BmeT110I CYCGRG 1 cut(s) 57
BmgBI CACGTC 1 cut(s) 949
BmgT120I GGNCC 1 cut(s) 170
BmrFI CCNGG 1 cut(s) 141
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 5 cut(s) 253, 554, 648, 763, 1063
BmtI GCTAGC 1 cut(s) 159
BmuI ACTGGG 1 cut(s) 91
BpiI GAAGAC 1 cut(s) 842
BplI GAGNNNNNCTC 2 cut(s) 143, 175
BpmI CTGGAG 3 cut(s) 30, 339, 804
Bpu14I TTCGAA 1 cut(s) 971
BpuEI CTTGAG 2 cut(s) 122, 357
Bsa29I ATCGAT 1 cut(s) 477
BsaBI GATNNNNATC 1 cut(s) 639
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 3 cut(s) 33, 856, 886
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 806
Bse1I ACTGG 2 cut(s) 13, 86
Bse3DI GCAATG 1 cut(s) 719
Bse8I GATNNNNATC 1 cut(s) 639
BseAI TCCGGA 1 cut(s) 319
BseBI CCWGG 1 cut(s) 141
BseCI ATCGAT 1 cut(s) 477
BseGI GGATG 3 cut(s) 49, 240, 1110
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 2 cut(s) 146, 806
BseMI GCAATG 1 cut(s) 719
BseMII CTCAG 3 cut(s) 480, 678, 948
BseNI ACTGG 2 cut(s) 13, 86
BseRI GAGGAG 2 cut(s) 278, 360
BseXI GCAGC 5 cut(s) 330, 598, 872, 973, 976
BshFI GGCC 2 cut(s) 639, 1095
BshVI ATCGAT 1 cut(s) 477
BsiHKAI GWGCWC 2 cut(s) 368, 926
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 320
BslFI GGGAC 1 cut(s) 829
BslI CCNNNNNNNGG 2 cut(s) 146, 806
BsmAI GTCTC 1 cut(s) 491
BsmFI GGGAC 1 cut(s) 829
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 2 cut(s) 639, 1095
BsoBI CYCGRG 1 cut(s) 57
Bsp119I TTCGAA 1 cut(s) 971
Bsp1286I GDGCHC 2 cut(s) 368, 926
Bsp13I TCCGGA 1 cut(s) 319
Bsp143I GATC 7 cut(s) 193, 397, 445, 478, 759, 826, 1107
BspACI CCGC 4 cut(s) 494, 567, 673, 934
BspANI GGCC 2 cut(s) 639, 1095
BspCNI CTCAG 3 cut(s) 481, 679, 949
BspDI ATCGAT 1 cut(s) 477
BspEI TCCGGA 1 cut(s) 319
BspOI GCTAGC 1 cut(s) 159
BspPI GGATC 2 cut(s) 405, 473
BspT104I TTCGAA 1 cut(s) 971
BsrDI GCAATG 1 cut(s) 719
BsrI ACTGG 2 cut(s) 13, 86
BssMI GATC 7 cut(s) 193, 397, 445, 478, 759, 826, 1107
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 363
Bst6I CTCTTC 1 cut(s) 648
BstBI TTCGAA 1 cut(s) 971
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 3 cut(s) 489, 687, 957
BstEII GGTNACC 1 cut(s) 469
BstF5I GGATG 3 cut(s) 49, 240, 1110
BstKTI GATC 7 cut(s) 196, 400, 448, 481, 762, 829, 1110
BstMAI GTCTC 1 cut(s) 491
BstMBI GATC 7 cut(s) 193, 397, 445, 478, 759, 826, 1107
BstMWI GCNNNNNNNGC 2 cut(s) 343, 551
BstNI CCWGG 1 cut(s) 141
BstPI GGTNACC 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 139
BstV1I GCAGC 5 cut(s) 330, 598, 872, 973, 976
BstV2I GAAGAC 1 cut(s) 842
BstX2I RGATCY 2 cut(s) 397, 445
BstXI CCANNNNNNTGG 1 cut(s) 251
BstYI RGATCY 2 cut(s) 397, 445
Bsu15I ATCGAT 1 cut(s) 477
BsuI GTATCC 2 cut(s) 79, 693
BsuRI GGCC 2 cut(s) 639, 1095
BsuTUI ATCGAT 1 cut(s) 477
BtrI CACGTC 1 cut(s) 949
BtsCI GGATG 3 cut(s) 49, 240, 1110
BtsIMutI CAGTG 1 cut(s) 368
Cac8I GCNNGC 1 cut(s) 157
CaiI CAGNNNCTG 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 170
ClaI ATCGAT 1 cut(s) 477
Csp6I GTAC 3 cut(s) 529, 663, 780
CviAII CATG 3 cut(s) 208, 251, 749
CviQI GTAC 3 cut(s) 529, 663, 780
DdeI CTNAG 3 cut(s) 489, 687, 957
DpnI GATC 7 cut(s) 195, 399, 447, 480, 761, 828, 1109
DpnII GATC 7 cut(s) 193, 397, 445, 478, 759, 826, 1107
EaeI YGGCCR 1 cut(s) 637
Eam1104I CTCTTC 1 cut(s) 648
EarI CTCTTC 1 cut(s) 648
EciI GGCGGA 1 cut(s) 509
Eco47I GGWCC 1 cut(s) 170
Eco57I CTGAAG 2 cut(s) 114, 393
Eco88I CYCGRG 1 cut(s) 57
Eco91I GGTNACC 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 170
EcoO65I GGTNACC 1 cut(s) 469
EcoRI GAATTC 1 cut(s) 1035
EcoRII CCWGG 1 cut(s) 139
EcoT22I ATGCAT 1 cut(s) 1078
FaeI CATG 3 cut(s) 211, 254, 752
FaqI GGGAC 1 cut(s) 829
FatI CATG 3 cut(s) 207, 250, 748
FauI CCCGC 1 cut(s) 941
FbaI TGATCA 1 cut(s) 193
Fnu4HI GCNGC 5 cut(s) 344, 612, 861, 987, 990
FokI GGATG 3 cut(s) 56, 227, 1117
Fsp4HI GCNGC 5 cut(s) 344, 612, 861, 987, 990
FspBI CTAG 2 cut(s) 156, 431
GluI GCNGC 5 cut(s) 344, 612, 861, 987, 990
GsuI CTGGAG 3 cut(s) 30, 339, 804
HaeIII GGCC 2 cut(s) 639, 1095
HapII CCGG 1 cut(s) 320
Hin1II CATG 3 cut(s) 211, 254, 752
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 7 cut(s) 269, 392, 575, 796, 817, 904, 968
HpaII CCGG 1 cut(s) 320
HphI GGTGA 1 cut(s) 80
Hpy166II GTNNAC 4 cut(s) 6, 79, 665, 1012
Hpy188I TCNGA 8 cut(s) 193, 397, 412, 445, 460, 490, 746, 1112
Hpy8I GTNNAC 4 cut(s) 6, 79, 665, 1012
HpyAV CCTTC 2 cut(s) 183, 1061
HpyCH4III ACNGT 1 cut(s) 363
HpyCH4IV ACGT 1 cut(s) 948
HpyCH4V TGCA 4 cut(s) 266, 346, 992, 1076
HpyF10VI GCNNNNNNNGC 2 cut(s) 343, 551
HpyF3I CTNAG 3 cut(s) 489, 687, 957
HpySE526I ACGT 1 cut(s) 948
Hsp92II CATG 3 cut(s) 211, 254, 752
Kpn2I TCCGGA 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 193
Kzo9I GATC 7 cut(s) 193, 397, 445, 478, 759, 826, 1107
LmnI GCTCC 3 cut(s) 322, 423, 921
Lsp1109I GCAGC 5 cut(s) 330, 598, 872, 973, 976
LweI GCATC 5 cut(s) 253, 554, 648, 763, 1063
MaeI CTAG 2 cut(s) 156, 431
MaeII ACGT 1 cut(s) 948
MaeIII GTNAC 2 cut(s) 469, 579
MalI GATC 7 cut(s) 195, 399, 447, 480, 761, 828, 1109
MboI GATC 7 cut(s) 193, 397, 445, 478, 759, 826, 1107
MflI RGATCY 2 cut(s) 397, 445
MhlI GDGCHC 2 cut(s) 368, 926
MlsI TGGCCA 1 cut(s) 639
MluCI AATT 7 cut(s) 273, 387, 536, 618, 713, 783, 1035
MluNI TGGCCA 1 cut(s) 639
MlyI GAGTC 4 cut(s) 386, 569, 811, 898
Mox20I TGGCCA 1 cut(s) 639
Mph1103I ATGCAT 1 cut(s) 1078
MroI TCCGGA 1 cut(s) 319
MscI TGGCCA 1 cut(s) 639
MseI TTAA 1 cut(s) 519
Msp20I TGGCCA 1 cut(s) 639
MspA1I CMGCKG 2 cut(s) 611, 824
MspI CCGG 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 141
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 343, 551
NdeII GATC 7 cut(s) 193, 397, 445, 478, 759, 826, 1107
NheI GCTAGC 1 cut(s) 155
NlaIII CATG 3 cut(s) 211, 254, 752
NmuCI GTSAC 1 cut(s) 579
NsiI ATGCAT 1 cut(s) 1078
NspV TTCGAA 1 cut(s) 971
PctI GAATGC 1 cut(s) 346
PfeI GAWTC 3 cut(s) 269, 796, 968
PflMI CCANNNNNTGG 2 cut(s) 146, 806
PkrI GCNGC 5 cut(s) 345, 613, 862, 988, 991
PleI GAGTC 4 cut(s) 386, 569, 811, 898
PpsI GAGTC 4 cut(s) 386, 569, 811, 898
PpuMI RGGWCCY 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 170
Psp6I CCWGG 1 cut(s) 139
PspEI GGTNACC 1 cut(s) 469
PspGI CCWGG 1 cut(s) 139
PspPI GGNCC 1 cut(s) 170
PspPPI RGGWCCY 1 cut(s) 170
PstNI CAGNNNCTG 1 cut(s) 146
PsuI RGATCY 2 cut(s) 397, 445
PvuII CAGCTG 2 cut(s) 611, 824
RsaI GTAC 3 cut(s) 530, 664, 781
RsaNI GTAC 3 cut(s) 529, 663, 780
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 5 cut(s) 344, 612, 861, 987, 990
Sau3AI GATC 7 cut(s) 193, 397, 445, 478, 759, 826, 1107
Sau96I GGNCC 1 cut(s) 170
SchI GAGTC 4 cut(s) 386, 569, 811, 898
ScrFI CCNGG 1 cut(s) 141
SduI GDGCHC 2 cut(s) 368, 926
SfaNI GCATC 5 cut(s) 253, 554, 648, 763, 1063
SfuI TTCGAA 1 cut(s) 971
SinI GGWCC 1 cut(s) 170
SmlI CTYRAG 2 cut(s) 101, 372
SmoI CTYRAG 2 cut(s) 101, 372
Sse9I AATT 7 cut(s) 273, 387, 536, 618, 713, 783, 1035
SsiI CCGC 4 cut(s) 494, 567, 673, 934
SspMI CTAG 2 cut(s) 156, 431
StyD4I CCNGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 363
TaiI ACGT 1 cut(s) 951
TaqI TCGA 3 cut(s) 450, 477, 971
TasI AATT 7 cut(s) 273, 387, 536, 618, 713, 783, 1035
TfiI GAWTC 3 cut(s) 269, 796, 968
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 1 cut(s) 368
TseFI GTSAC 1 cut(s) 579
TseI GCWGC 5 cut(s) 343, 611, 860, 986, 989
Tsp45I GTSAC 1 cut(s) 579
TspDTI ATGAA 4 cut(s) 592, 708, 776, 818
TspRI CASTG 1 cut(s) 368
Van91I CCANNNNNTGG 2 cut(s) 146, 806
VpaK11BI GGWCC 1 cut(s) 170
XapI RAATTY 3 cut(s) 618, 713, 1035
XbaI TCTAGA 1 cut(s) 430
XspI CTAG 2 cut(s) 156, 431
Zsp2I ATGCAT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.