Rorug01G0041900

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0011604
Physical Location & Seq
Forward (+)
6896556 .. 6897437
882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0041900.1

Sequence Viewer

Length: 564 bp
ATGCGACCGCCTAAAGCAGCAAAGTCCAAGAAGGCTGTGGCATCAAAGAAGTCGTCATCTGGTAGTAAGTCGGCAAAGAGCAAGTCATCCGAGGATTCTGATGATGATGAGAATGTCACTGGTGGATTGAGGTTGCTTAAGCGAGGGATGGTAACCACGAATCGAATTACTAGGCGACTGCTTCGAGGAAAGAAGCTAAAGGTGAATTCTAATGCTGATGGGGAGCCAATTGGGAAGGCAGCTAAGGAGATGCAGTCCTACATTGGGGTGTTGGCACGCACCAAGATCCCAATATCTATAAATGATTGGAGAAATGTGCCTTTGGATGAAAAAGACAATATTTGGAATAGTGTTCAGGATGCTTATGTGGTGCCGAAAGAGTGGAAGAAACTGTTCCCTCCAGGTGATTATCGATCCATTGAGGTAGATGAATGGAATACGTTCGTGGCTGATAGAACTTCAAAACAATTTCAGGTACTCCGCAAAATGCAGAGAACAAAGAGGAAGGAGAATAAGTATCCTCATCGAATGTCACGGAAAGGATATGCTAACTTCCAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

187

Amino Acids

21.39

Weight (kDa)

10.19

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 370
AciI CCGC 2 cut(s) 8, 481
AclWI GGATC 2 cut(s) 280, 408
AcsI RAATTY 1 cut(s) 205
AfaI GTAC 1 cut(s) 477
AfiI CCNNNNNNNGG 1 cut(s) 264
AflII CTTAAG 1 cut(s) 137
AgsI TTSAA 1 cut(s) 462
AjnI CCWGG 2 cut(s) 400, 555
AluBI AGCT 2 cut(s) 196, 242
AluI AGCT 2 cut(s) 196, 242
AlwI GGATC 2 cut(s) 280, 408
ApeKI GCWGC 2 cut(s) 17, 239
ApoI RAATTY 1 cut(s) 205
ArsI GACNNNNNNTTYG 4 cut(s) 38, 68, 70, 100
Asp700I GAANNNNTTC 2 cut(s) 392, 440
AsuHPI GGTGA 2 cut(s) 214, 416
BanI GGYRCC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 29, 251
BccI CCATC 2 cut(s) 142, 212
BciT130I CCWGG 2 cut(s) 402, 557
BciVI GTATCC 1 cut(s) 528
BfaI CTAG 1 cut(s) 171
BfrI CTTAAG 1 cut(s) 137
BfuI GTATCC 1 cut(s) 528
BisI GCNGC 2 cut(s) 18, 240
BlsI GCNGC 2 cut(s) 19, 241
Bme1390I CCNGG 2 cut(s) 402, 557
BmiI GGNNCC 2 cut(s) 225, 372
BmrFI CCNGG 2 cut(s) 402, 557
BmsI GCATC 3 cut(s) 50, 240, 349
BpmI CTGGAG 1 cut(s) 384
Bpu10I CCTNAGC 1 cut(s) 243
Bsa29I ATCGAT 1 cut(s) 412
BsaJI CCNNGG 1 cut(s) 90
BsaXI ACNNNNNCTCC 4 cut(s) 239, 269, 500, 530
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse1I ACTGG 1 cut(s) 124
BseBI CCWGG 2 cut(s) 402, 557
BseCI ATCGAT 1 cut(s) 412
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 4 cut(s) 86, 153, 331, 364
BseLI CCNNNNNNNGG 1 cut(s) 264
BseNI ACTGG 1 cut(s) 124
BseXI GCAGC 2 cut(s) 29, 251
Bsh1285I CGRYCG 1 cut(s) 8
BshNI GGYRCC 1 cut(s) 370
BshVI ATCGAT 1 cut(s) 412
BsiEI CGRYCG 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 264
Bsp143I GATC 2 cut(s) 285, 413
BspACI CCGC 2 cut(s) 8, 481
BspDI ATCGAT 1 cut(s) 412
BspLI GGNNCC 2 cut(s) 225, 372
BspPI GGATC 2 cut(s) 280, 408
BspT107I GGYRCC 1 cut(s) 370
BspTI CTTAAG 1 cut(s) 137
BsrI ACTGG 1 cut(s) 124
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 2 cut(s) 285, 413
Bst2UI CCWGG 2 cut(s) 402, 557
Bst4CI ACNGT 1 cut(s) 393
BstAFI CTTAAG 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 1 cut(s) 243
BstEII GGTNACC 1 cut(s) 151
BstF5I GGATG 4 cut(s) 86, 153, 331, 364
BstKTI GATC 2 cut(s) 288, 416
BstMBI GATC 2 cut(s) 285, 413
BstMCI CGRYCG 1 cut(s) 8
BstNI CCWGG 2 cut(s) 402, 557
BstPI GGTNACC 1 cut(s) 151
BstSCI CCNGG 2 cut(s) 400, 555
BstV1I GCAGC 2 cut(s) 29, 251
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
Bsu15I ATCGAT 1 cut(s) 412
BsuI GTATCC 1 cut(s) 528
BsuTUI ATCGAT 1 cut(s) 412
BtsCI GGATG 4 cut(s) 86, 153, 331, 364
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 412
Csp6I GTAC 1 cut(s) 476
CviJI RGCY 5 cut(s) 35, 196, 226, 242, 449
CviKI_1 RGCY 5 cut(s) 35, 196, 226, 242, 449
CviQI GTAC 1 cut(s) 476
DdeI CTNAG 1 cut(s) 243
DpnI GATC 2 cut(s) 287, 415
DpnII GATC 2 cut(s) 285, 413
Eco91I GGTNACC 1 cut(s) 151
EcoO65I GGTNACC 1 cut(s) 151
EcoRI GAATTC 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 400, 555
FaiI YATR 3 cut(s) 299, 366, 546
Fnu4HI GCNGC 2 cut(s) 18, 240
FokI GGATG 4 cut(s) 73, 160, 338, 371
Fsp4HI GCNGC 2 cut(s) 18, 240
FspBI CTAG 1 cut(s) 171
GluI GCNGC 2 cut(s) 18, 240
GsuI CTGGAG 1 cut(s) 384
HinfI GANTC 2 cut(s) 95, 160
HphI GGTGA 2 cut(s) 214, 416
Hpy188I TCNGA 2 cut(s) 91, 100
Hpy188III TCNNGA 1 cut(s) 356
HpyAV CCTTC 3 cut(s) 25, 229, 499
HpyCH4III ACNGT 1 cut(s) 393
HpyCH4IV ACGT 1 cut(s) 440
HpyCH4V TGCA 2 cut(s) 253, 490
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 440
Kzo9I GATC 2 cut(s) 285, 413
LmnI GCTCC 1 cut(s) 223
LpnPI CCDG 7 cut(s) 45, 105, 341, 387, 414, 458, 542
Lsp1109I GCAGC 2 cut(s) 29, 251
LweI GCATC 3 cut(s) 50, 240, 349
MaeI CTAG 1 cut(s) 171
MaeII ACGT 1 cut(s) 440
MaeIII GTNAC 3 cut(s) 115, 151, 531
MalI GATC 2 cut(s) 287, 415
MboI GATC 2 cut(s) 285, 413
MboII GAAGA 1 cut(s) 397
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 285
MluCI AATT 4 cut(s) 165, 205, 228, 467
MnlI CCTC 8 cut(s) 85, 123, 137, 179, 408, 415, 495, 531
MroXI GAANNNNTTC 2 cut(s) 392, 440
MseI TTAA 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 266
MspCI CTTAAG 1 cut(s) 137
MspR9I CCNGG 2 cut(s) 402, 557
MunI CAATTG 1 cut(s) 228
MvaI CCWGG 2 cut(s) 402, 557
NdeII GATC 2 cut(s) 285, 413
NlaIV GGNNCC 2 cut(s) 225, 372
NmuCI GTSAC 2 cut(s) 115, 531
PdmI GAANNNNTTC 2 cut(s) 392, 440
PfeI GAWTC 2 cut(s) 95, 160
PfoI TCCNGGA 1 cut(s) 555
PkrI GCNGC 2 cut(s) 19, 241
Psp6I CCWGG 2 cut(s) 400, 555
PspEI GGTNACC 1 cut(s) 151
PspGI CCWGG 2 cut(s) 400, 555
PspN4I GGNNCC 2 cut(s) 225, 372
PsuI RGATCY 1 cut(s) 285
RsaI GTAC 1 cut(s) 477
RsaNI GTAC 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 2 cut(s) 18, 240
Sau3AI GATC 2 cut(s) 285, 413
ScrFI CCNGG 2 cut(s) 402, 557
SetI ASST 8 cut(s) 134, 198, 204, 244, 406, 426, 443, 477
SfaNI GCATC 3 cut(s) 50, 240, 349
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 4 cut(s) 165, 205, 228, 467
SsiI CCGC 2 cut(s) 8, 481
SspI AATATT 1 cut(s) 340
SspMI CTAG 1 cut(s) 171
StyD4I CCNGG 2 cut(s) 400, 555
TaaI ACNGT 1 cut(s) 393
TaiI ACGT 1 cut(s) 443
TaqI TCGA 4 cut(s) 163, 184, 412, 526
TasI AATT 4 cut(s) 165, 205, 228, 467
TfiI GAWTC 2 cut(s) 95, 160
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 2 cut(s) 115, 531
TseI GCWGC 2 cut(s) 17, 239
Tsp45I GTSAC 2 cut(s) 115, 531
TspDTI ATGAA 2 cut(s) 342, 444
TspGWI ACGGA 1 cut(s) 550
TspRI CASTG 1 cut(s) 124
Vha464I CTTAAG 1 cut(s) 137
XapI RAATTY 1 cut(s) 205
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XmnI GAANNNNTTC 2 cut(s) 392, 440
XspI CTAG 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.