Rh4BG286600

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011604
Physical Location & Seq
Forward (+)
45998627 .. 46001036
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG286600.1

Sequence Viewer

Length: 615 bp
ATGGGGGTAATCCAAGACCTTAATGGAGATAGAATAAATGCCCTACGATTTCTTACCAGAGGAAAGACCATTTGTGAGGAAAGGATTCCAGTAATTGGACTTGCATTTTCTTCTGCCTTAGGAAAAGTGTATTGTAAAGAGCAAAATCTCAAGTCAGCTAAAAGTGAATTCCGAGACACTGTCGCCTTATTCAAGTGTATGGAAGACTTTCCCTGCGAAAAGTGCAAACTAAGGTTGGCGGGTACTATTTTTCAGAACACACTACTGTTTTTCTATAATAGAAAAACTGCATCACTAGTGAAGGTATGTGATGTTATAGAGAAGTATAAAGTAGCCCACAGTAAACTAAATGATTCGGAATGGAGAAACAGTATTAGCTGCAATGATTATAATTCTCATTTGGAGGTTAATATGAATTGCATCTGCAAGACTAGCGGTTGTTGGCAATGTTTGCTTCGAGGAGTTGTCAAATCTACCTCAGTGATCGATTTTGTTCATCTAAAATGGGAATCGGTGCGGAGACGCCTATCTCTCCAGCTATTTTCAGGATTAGGAAAGTTGTTTGAGAACAACACAGAGCTTACAGATGATATTTCAGAAGCATGCCATTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

204

Amino Acids

23.27

Weight (kDa)

8.66

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 390
AccB7I CCANNNNNTGG 1 cut(s) 95
AciI CCGC 3 cut(s) 239, 435, 517
AcsI RAATTY 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 523
AfaI GTAC 1 cut(s) 244
AfiI CCNNNNNNNGG 1 cut(s) 95
AgsI TTSAA 1 cut(s) 193
AhlI ACTAGT 1 cut(s) 295
AluBI AGCT 4 cut(s) 158, 378, 538, 580
AluI AGCT 4 cut(s) 158, 378, 538, 580
Alw26I GTCTC 2 cut(s) 168, 514
ApeKI GCWGC 1 cut(s) 378
ApoI RAATTY 1 cut(s) 167
Asp700I GAANNNNTTC 2 cut(s) 84, 207
AxyI CCTNAGG 1 cut(s) 118
BbsI GAAGAC 1 cut(s) 210
BbvI GCAGC 1 cut(s) 365
BcoDI GTCTC 2 cut(s) 168, 514
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 2 cut(s) 296, 432
BisI GCNGC 1 cut(s) 379
BlsI GCNGC 1 cut(s) 380
BmsI GCATC 2 cut(s) 299, 429
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 134
Bsa29I ATCGAT 1 cut(s) 486
BsaHI GRCGYC 1 cut(s) 523
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse1I ACTGG 1 cut(s) 89
Bse21I CCTNAGG 1 cut(s) 118
Bse3DI GCAATG 3 cut(s) 388, 452, 607
BseCI ATCGAT 1 cut(s) 486
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMI GCAATG 3 cut(s) 388, 452, 607
BseMII CTCAG 1 cut(s) 492
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 1 cut(s) 474
BseXI GCAGC 1 cut(s) 365
BshVI ATCGAT 1 cut(s) 486
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 2 cut(s) 168, 514
BsmBI CGTCTC 1 cut(s) 514
Bsp143I GATC 1 cut(s) 483
BspACI CCGC 3 cut(s) 239, 435, 517
BspCNI CTCAG 1 cut(s) 491
BspDI ATCGAT 1 cut(s) 486
BsrDI GCAATG 3 cut(s) 388, 452, 607
BsrI ACTGG 1 cut(s) 89
BssMI GATC 1 cut(s) 483
BssNI GRCGYC 1 cut(s) 523
Bst4CI ACNGT 4 cut(s) 181, 267, 341, 371
BstACI GRCGYC 1 cut(s) 523
BstAPI GCANNNNNTGC 1 cut(s) 451
BstC8I GCNNGC 1 cut(s) 604
BstDEI CTNAG 3 cut(s) 118, 230, 478
BstKTI GATC 1 cut(s) 486
BstMAI GTCTC 2 cut(s) 168, 514
BstMBI GATC 1 cut(s) 483
BstMWI GCNNNNNNNGC 3 cut(s) 222, 432, 451
BstNSI RCATGY 1 cut(s) 606
BstV1I GCAGC 1 cut(s) 365
BstV2I GAAGAC 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 486
Bsu36I CCTNAGG 1 cut(s) 118
BsuTUI ATCGAT 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 177, 486
Cac8I GCNNGC 1 cut(s) 604
ClaI ATCGAT 1 cut(s) 486
CseI GACGC 1 cut(s) 531
Csp6I GTAC 1 cut(s) 243
CviAII CATG 1 cut(s) 603
CviJI RGCY 5 cut(s) 158, 335, 378, 538, 580
CviKI_1 RGCY 5 cut(s) 158, 335, 378, 538, 580
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 3 cut(s) 118, 230, 478
DpnI GATC 1 cut(s) 485
DpnII GATC 1 cut(s) 483
Eco81I CCTNAGG 1 cut(s) 118
EcoRI GAATTC 1 cut(s) 167
Esp3I CGTCTC 1 cut(s) 514
FaeI CATG 1 cut(s) 606
FaiI YATR 8 cut(s) 200, 276, 307, 317, 327, 390, 413, 604
FatI CATG 1 cut(s) 602
FauI CCCGC 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 379
Fsp4HI GCNGC 1 cut(s) 379
FspBI CTAG 2 cut(s) 296, 432
GluI GCNGC 1 cut(s) 379
GsuI CTGGAG 1 cut(s) 518
HgaI GACGC 1 cut(s) 531
Hin1I GRCGYC 1 cut(s) 523
Hin1II CATG 1 cut(s) 606
HinfI GANTC 3 cut(s) 85, 353, 509
Hpy166II GTNNAC 1 cut(s) 344
Hpy188I TCNGA 4 cut(s) 173, 255, 358, 598
Hpy188III TCNNGA 1 cut(s) 546
Hpy8I GTNNAC 1 cut(s) 344
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 4 cut(s) 181, 267, 341, 371
HpyCH4V TGCA 6 cut(s) 104, 225, 290, 381, 420, 426
HpyF10VI GCNNNNNNNGC 3 cut(s) 222, 432, 451
HpyF3I CTNAG 3 cut(s) 118, 230, 478
Hsp92I GRCGYC 1 cut(s) 523
Hsp92II CATG 1 cut(s) 606
Kzo9I GATC 1 cut(s) 483
LpnPI CCDG 5 cut(s) 70, 102, 226, 531, 548
Lsp1109I GCAGC 1 cut(s) 365
LweI GCATC 2 cut(s) 299, 429
MaeI CTAG 2 cut(s) 296, 432
MalI GATC 1 cut(s) 485
MboI GATC 1 cut(s) 483
MboII GAAGA 2 cut(s) 102, 215
MluCI AATT 4 cut(s) 93, 167, 391, 415
MnlI CCTC 5 cut(s) 53, 70, 397, 452, 487
MroXI GAANNNNTTC 2 cut(s) 84, 207
MseI TTAA 2 cut(s) 21, 408
MwoI GCNNNNNNNGC 3 cut(s) 222, 432, 451
NdeII GATC 1 cut(s) 483
NlaIII CATG 1 cut(s) 606
NspI RCATGY 1 cut(s) 606
PaeI GCATGC 1 cut(s) 606
PdmI GAANNNNTTC 2 cut(s) 84, 207
PfeI GAWTC 3 cut(s) 85, 353, 509
PflFI GACNNNGTC 1 cut(s) 179
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 1 cut(s) 380
PsiI TTATAA 1 cut(s) 390
PsyI GACNNNGTC 1 cut(s) 179
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 21, 408
SatI GCNGC 1 cut(s) 379
Sau3AI GATC 1 cut(s) 483
SetI ASST 9 cut(s) 21, 160, 236, 306, 380, 408, 479, 540, 582
SfaNI GCATC 2 cut(s) 299, 429
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SpeI ACTAGT 1 cut(s) 295
SphI GCATGC 1 cut(s) 606
Sse9I AATT 4 cut(s) 93, 167, 391, 415
SsiI CCGC 3 cut(s) 239, 435, 517
SspMI CTAG 2 cut(s) 296, 432
TaaI ACNGT 4 cut(s) 181, 267, 341, 371
TaqI TCGA 2 cut(s) 457, 486
TasI AATT 4 cut(s) 93, 167, 391, 415
TfiI GAWTC 3 cut(s) 85, 353, 509
Tru1I TTAA 2 cut(s) 21, 408
Tru9I TTAA 2 cut(s) 21, 408
TscAI CASTG 2 cut(s) 184, 486
TseI GCWGC 1 cut(s) 378
TspDTI ATGAA 2 cut(s) 428, 485
TspRI CASTG 2 cut(s) 184, 486
Tth111I GACNNNGTC 1 cut(s) 179
Van91I CCANNNNNTGG 1 cut(s) 95
XapI RAATTY 1 cut(s) 167
XceI RCATGY 1 cut(s) 606
XcmI CCANNNNNNNNNTGG 1 cut(s) 20
XmnI GAANNNNTTC 2 cut(s) 84, 207
XspI CTAG 2 cut(s) 296, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.