Rh4AG281100

separase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011604
Physical Location & Seq
Forward (+)
59626992 .. 59641599
14608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG281100.1

Sequence Viewer

Length: 1317 bp
ATGGGGGTAATCCAAGACCTTAATGGAGATAGAATAAATGCCCTACGATTTCTTACCAGAGGAAAGACCATTTGTGAGGAAAGGATTCCAGTAATTGGACTTGCATTTTCTTCTGCCTTAGGAAAAGTGTATTGTAAAGAGCAAAATCTCAAGTCAGCTAAAAGTGAATTCCGAGACACTGTCGCCTTATTCAAGTGTATGGAAGACTTTCCCTGCGAAAAGTGCAAACTAAGTGGGAATGCATTTTCTCCTGCTATCTCTACCGATCTTTTCCTTGACACAATGGGACAAGATATTAAGGGATTTGATCTTTTTGTTGAGCTTGCAGAACTACTCTACAACATATTTTGGTGGTGTTTGCAAGCTCGTTCGGACATAACTAGCCGGATTATCTTTTCTGATTTGGCTGATAATGATCTTGCAAAGAATCTGTCTAGACTGTTATCTTGGTTGGTCATGGCATTTGTGACAAGCAAAGAAGTTCCAGTACTTTTTCAGAAGGTTGCCAAACTACTTGCTATGATTTGTTCATTGAACTATGCTACTCTTGACCATGACAAGCATTCTCCTTTTCTGTCTTGCCGAGATCTCGGGTATTTCACTCCAGAATGGGCATCATATTTCCATCAAGCTTCCTTGGGTTCAGATATCCCTAGTTCAACAAAACGCAATCTTGCACCACAAAAGGTTGCTGATCTTGGATCATTTGTAAAAAGTTTTTTCGAACGCCTCCCTTCGACACCAGTAATTTGTGTAAGTGTGATTGGTGGCTCGTATGGTAATCTTTTGCAACAATTGGTGTCTACTGATTCTTCTCTTCAAGCATGGATTTTAATAACACGCCTTAGGTCGAATCAGAAACCTATTAATTTGCTTCTACCAATTCCGGAAATGATTGAAGGAGGAAAACGCGACTGGGACAAGAATCATGAGAATGTTGAAAGTTGGCTCTGTCCATGGGGTTCCGAAGGTTCTACTGAAATTGAGGATATAATACCTGAATATAGGATCATAATGGAGGGGGTTCACTTGTTAGAACAGCATGTTGACGTTCGGCCGCTTGATCATCGGCTAAAGACATTTATCAGCCGGACTATCTTTTCTGATTTGGCTGATAATGATCTTGCCAAGAATCTGCCTAGACTGTTATCTTGGTTGGTCCTGGCATTTGTGACAAGCAGAGAAGTTCCAGTACTTTTTCAGAAGGGAATTTGGGAATATTCGAACGTGTCTTTCTTTCTTTTATCTCCTTCCTCACCTTCCTCAGCACTCGGCACAAAAGAACCACCACCAGTGGAAAAGACCCTAGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000278 GO:0000280 GO:0000281 GO:0000902 GO:0000910 GO:0000911 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006508 GO:0006807 GO:0006810 GO:0006887 GO:0006996 GO:0007049 GO:0007059 GO:0007063 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010564 GO:0010638 GO:0015630 GO:0016043 GO:0016049 GO:0016192 GO:0016787 GO:0019538 GO:0022402 GO:0022414 GO:0032501 GO:0032502 GO:0032506 GO:0032878 GO:0032940 GO:0032989 GO:0033043 GO:0033044 GO:0033045 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044238 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045132 GO:0045787 GO:0045876 GO:0046903 GO:0048285 GO:0048316 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0051301 GO:0051304 GO:0051307 GO:0051321 GO:0051726 GO:0051983 GO:0051984 GO:0060560 GO:0061458 GO:0061640 GO:0065007 GO:0070011 GO:0071704 GO:0071840 GO:0072686 GO:0090068 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1902410 GO:1903046 GO:1903047 GO:2000114 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 map04110 map04111 map04113 map04114
Pfam Domains
Protein Families
Peptidase_C50

Protein Analysis

438

Amino Acids

49.31

Weight (kDa)

5.76

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AccI GTMKAC 1 cut(s) 803
AccII CGCG 1 cut(s) 912
AccIII TCCGGA 1 cut(s) 886
AciI CCGC 1 cut(s) 1058
AclWI GGATC 2 cut(s) 709, 1016
AcoI YGGCCR 1 cut(s) 1055
AcsI RAATTY 2 cut(s) 167, 1209
AfaI GTAC 2 cut(s) 489, 1194
AfiI CCNNNNNNNGG 1 cut(s) 95
AflIII ACRYGT 1 cut(s) 1227
AgsI TTSAA 6 cut(s) 193, 535, 660, 821, 899, 941
AjnI CCWGG 1 cut(s) 1161
AluBI AGCT 4 cut(s) 158, 322, 365, 632
AluI AGCT 4 cut(s) 158, 322, 365, 632
Alw26I GTCTC 1 cut(s) 168
AlwI GGATC 2 cut(s) 709, 1016
Ama87I CYCGRG 1 cut(s) 590
Aor13HI TCCGGA 1 cut(s) 886
AoxI GGCC 1 cut(s) 1055
ApoI RAATTY 2 cut(s) 167, 1209
ArsI GACNNNNNNTTYG 2 cut(s) 416, 448
AseI ATTAAT 1 cut(s) 867
Asp700I GAANNNNTTC 2 cut(s) 84, 207
AspS9I GGNCC 1 cut(s) 1159
AsuHPI GGTGA 1 cut(s) 1248
AsuII TTCGAA 2 cut(s) 723, 1223
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 1 cut(s) 1159
AxyI CCTNAGG 2 cut(s) 118, 845
BarI GAAGNNNNNNTAC 4 cut(s) 471, 503, 1176, 1208
BbsI GAAGAC 1 cut(s) 210
BbvCI CCTCAGC 1 cut(s) 1264
BccI CCATC 1 cut(s) 633
BciT130I CCWGG 1 cut(s) 1163
BclI TGATCA 1 cut(s) 1063
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 5 cut(s) 381, 435, 654, 1140, 1307
BglII AGATCT 1 cut(s) 586
BisI GCNGC 1 cut(s) 1058
BlsI GCNGC 1 cut(s) 1059
BmcAI AGTACT 2 cut(s) 489, 1194
Bme1390I CCNGG 1 cut(s) 1163
Bme18I GGWCC 1 cut(s) 1159
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 1 cut(s) 1159
BmiI GGNNCC 1 cut(s) 964
BmrFI CCNGG 1 cut(s) 1163
BmrI ACTGGG 1 cut(s) 925
BmsI GCATC 1 cut(s) 623
BmuI ACTGGG 1 cut(s) 925
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 588
Bpu10I CCTNAGC 1 cut(s) 1264
Bpu14I TTCGAA 2 cut(s) 723, 1223
BpuEI CTTGAG 1 cut(s) 134
BsaBI GATNNNNATC 2 cut(s) 414, 1119
BsaJI CCNNGG 2 cut(s) 636, 956
BsaWI WCCGGW 1 cut(s) 886
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse1I ACTGG 6 cut(s) 89, 485, 743, 920, 1190, 1292
Bse21I CCTNAGG 2 cut(s) 118, 845
Bse8I GATNNNNATC 2 cut(s) 414, 1119
BseAI TCCGGA 1 cut(s) 886
BseBI CCWGG 1 cut(s) 1163
BseDI CCNNGG 2 cut(s) 636, 956
BseJI GATNNNNATC 2 cut(s) 414, 1119
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMII CTCAG 1 cut(s) 1278
BseNI ACTGG 6 cut(s) 89, 485, 743, 920, 1190, 1292
BseX3I CGGCCG 1 cut(s) 1055
Bsh1236I CGCG 1 cut(s) 912
Bsh1285I CGRYCG 1 cut(s) 1058
BshFI GGCC 1 cut(s) 1057
BsiEI CGRYCG 1 cut(s) 1058
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 3 cut(s) 385, 887, 1090
BslFI GGGAC 2 cut(s) 300, 932
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 1 cut(s) 168
BsmFI GGGAC 2 cut(s) 300, 932
BsmI GAATGC 2 cut(s) 244, 562
BsnI GGCC 1 cut(s) 1057
BsoBI CYCGRG 1 cut(s) 590
Bsp119I TTCGAA 2 cut(s) 723, 1223
Bsp13I TCCGGA 1 cut(s) 886
Bsp143I GATC 9 cut(s) 265, 307, 415, 586, 694, 701, 1008, 1063, 1120
Bsp19I CCATGG 1 cut(s) 956
BspACI CCGC 1 cut(s) 1058
BspANI GGCC 1 cut(s) 1057
BspCNI CTCAG 1 cut(s) 1277
BspEI TCCGGA 1 cut(s) 886
BspFNI CGCG 1 cut(s) 912
BspHI TCATGA 1 cut(s) 928
BspLI GGNNCC 1 cut(s) 964
BspPI GGATC 2 cut(s) 709, 1016
BspT104I TTCGAA 2 cut(s) 723, 1223
BsrI ACTGG 6 cut(s) 89, 485, 743, 920, 1190, 1292
BssECI CCNNGG 2 cut(s) 636, 956
BssMI GATC 9 cut(s) 265, 307, 415, 586, 694, 701, 1008, 1063, 1120
BssT1I CCWWGG 2 cut(s) 636, 956
Bst2UI CCWGG 1 cut(s) 1163
Bst4CI ACNGT 3 cut(s) 181, 441, 1146
Bst6I CTCTTC 1 cut(s) 822
BstBI TTCGAA 2 cut(s) 723, 1223
BstC8I GCNNGC 2 cut(s) 324, 363
BstDEI CTNAG 4 cut(s) 118, 230, 845, 1264
BstDSI CCRYGG 1 cut(s) 956
BstFNI CGCG 1 cut(s) 912
BstKTI GATC 9 cut(s) 268, 310, 418, 589, 697, 704, 1011, 1066, 1123
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 9 cut(s) 265, 307, 415, 586, 694, 701, 1008, 1063, 1120
BstMCI CGRYCG 1 cut(s) 1058
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstNI CCWGG 1 cut(s) 1163
BstNSI RCATGY 1 cut(s) 1046
BstSCI CCNGG 1 cut(s) 1161
BstUI CGCG 1 cut(s) 912
BstV2I GAAGAC 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BstZI CGGCCG 1 cut(s) 1055
Bsu36I CCTNAGG 2 cut(s) 118, 845
BsuRI GGCC 1 cut(s) 1057
BtgI CCRYGG 1 cut(s) 956
BtsIMutI CAGTG 2 cut(s) 177, 1299
Cac8I GCNNGC 2 cut(s) 324, 363
CciI TCATGA 1 cut(s) 928
Cfr13I GGNCC 1 cut(s) 1159
Csp6I GTAC 2 cut(s) 488, 1193
CspCI CAANNNNNGTGG 2 cut(s) 214, 249
CviAII CATG 6 cut(s) 457, 554, 825, 929, 957, 1043
CviQI GTAC 2 cut(s) 488, 1193
DdeI CTNAG 4 cut(s) 118, 230, 845, 1264
DpnI GATC 9 cut(s) 267, 309, 417, 588, 696, 703, 1010, 1065, 1122
DpnII GATC 9 cut(s) 265, 307, 415, 586, 694, 701, 1008, 1063, 1120
EaeI YGGCCR 1 cut(s) 1055
EagI CGGCCG 1 cut(s) 1055
Eam1104I CTCTTC 1 cut(s) 822
EarI CTCTTC 1 cut(s) 822
EclXI CGGCCG 1 cut(s) 1055
Eco130I CCWWGG 2 cut(s) 636, 956
Eco32I GATATC 1 cut(s) 649
Eco47I GGWCC 1 cut(s) 1159
Eco52I CGGCCG 1 cut(s) 1055
Eco81I CCTNAGG 2 cut(s) 118, 845
Eco88I CYCGRG 1 cut(s) 590
EcoRI GAATTC 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 1161
EcoRV GATATC 1 cut(s) 649
EcoT14I CCWWGG 2 cut(s) 636, 956
EcoT22I ATGCAT 1 cut(s) 244
ErhI CCWWGG 2 cut(s) 636, 956
FaeI CATG 6 cut(s) 460, 557, 828, 932, 960, 1046
FaqI GGGAC 2 cut(s) 300, 932
FatI CATG 6 cut(s) 456, 553, 824, 928, 956, 1042
FbaI TGATCA 1 cut(s) 1063
FblI GTMKAC 1 cut(s) 803
Fnu4HI GCNGC 1 cut(s) 1058
Fsp4HI GCNGC 1 cut(s) 1058
FspBI CTAG 5 cut(s) 381, 435, 654, 1140, 1307
GluI GCNGC 1 cut(s) 1058
GsuI CTGGAG 1 cut(s) 588
HaeIII GGCC 1 cut(s) 1057
HapII CCGG 3 cut(s) 385, 887, 1090
Hin1II CATG 6 cut(s) 460, 557, 828, 932, 960, 1046
HincII GTYRAC 1 cut(s) 1048
HindII GTYRAC 1 cut(s) 1048
HindIII AAGCTT 1 cut(s) 630
HinfI GANTC 6 cut(s) 85, 427, 809, 853, 925, 1132
HpaII CCGG 3 cut(s) 385, 887, 1090
HphI GGTGA 1 cut(s) 1248
Hpy166II GTNNAC 3 cut(s) 804, 1027, 1048
Hpy188I TCNGA 9 cut(s) 173, 373, 400, 498, 646, 858, 967, 1105, 1203
Hpy188III TCNNGA 5 cut(s) 435, 548, 605, 887, 929
Hpy8I GTNNAC 3 cut(s) 804, 1027, 1048
HpyAV CCTTC 7 cut(s) 493, 744, 893, 962, 1198, 1260, 1269
HpyCH4III ACNGT 3 cut(s) 181, 441, 1146
HpyCH4IV ACGT 2 cut(s) 1050, 1227
HpyCH4V TGCA 8 cut(s) 104, 225, 242, 326, 361, 422, 677, 790
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpyF3I CTNAG 4 cut(s) 118, 230, 845, 1264
HpySE526I ACGT 2 cut(s) 1050, 1227
Hsp92II CATG 6 cut(s) 460, 557, 828, 932, 960, 1046
Kpn2I TCCGGA 1 cut(s) 886
Ksp22I TGATCA 1 cut(s) 1063
Kzo9I GATC 9 cut(s) 265, 307, 415, 586, 694, 701, 1008, 1063, 1120
LweI GCATC 1 cut(s) 623
MaeI CTAG 5 cut(s) 381, 435, 654, 1140, 1307
MaeII ACGT 2 cut(s) 1050, 1227
MaeIII GTNAC 2 cut(s) 466, 1171
MalI GATC 9 cut(s) 267, 309, 417, 588, 696, 703, 1010, 1065, 1122
MboI GATC 9 cut(s) 265, 307, 415, 586, 694, 701, 1008, 1063, 1120
MboII GAAGA 4 cut(s) 102, 215, 804, 809
MfeI CAATTG 1 cut(s) 794
MflI RGATCY 1 cut(s) 586
MluCI AATT 8 cut(s) 93, 167, 747, 794, 868, 882, 981, 1209
MnlI CCTC 8 cut(s) 53, 70, 740, 896, 979, 1012, 1264, 1273
Mph1103I ATGCAT 1 cut(s) 244
MroI TCCGGA 1 cut(s) 886
MroXI GAANNNNTTC 2 cut(s) 84, 207
MseI TTAA 4 cut(s) 21, 297, 833, 867
MslI CAYNNNNRTG 1 cut(s) 933
MspI CCGG 3 cut(s) 385, 887, 1090
MspR9I CCNGG 1 cut(s) 1163
MunI CAATTG 1 cut(s) 794
Mva1269I GAATGC 2 cut(s) 244, 562
MvaI CCWGG 1 cut(s) 1163
MvnI CGCG 1 cut(s) 912
MwoI GCNNNNNNNGC 1 cut(s) 222
NcoI CCATGG 1 cut(s) 956
NdeII GATC 9 cut(s) 265, 307, 415, 586, 694, 701, 1008, 1063, 1120
NlaIII CATG 6 cut(s) 460, 557, 828, 932, 960, 1046
NlaIV GGNNCC 1 cut(s) 964
NmeAIII GCCGAG 2 cut(s) 608, 1251
NmuCI GTSAC 2 cut(s) 466, 1171
NsiI ATGCAT 1 cut(s) 244
NspI RCATGY 1 cut(s) 1046
NspV TTCGAA 2 cut(s) 723, 1223
PagI TCATGA 1 cut(s) 928
PctI GAATGC 2 cut(s) 244, 562
PdmI GAANNNNTTC 2 cut(s) 84, 207
PfeI GAWTC 6 cut(s) 85, 427, 809, 853, 925, 1132
PflFI GACNNNGTC 1 cut(s) 179
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 1 cut(s) 1059
PshBI ATTAAT 1 cut(s) 867
Psp6I CCWGG 1 cut(s) 1161
PspGI CCWGG 1 cut(s) 1161
PspN4I GGNNCC 1 cut(s) 964
PspPI GGNCC 1 cut(s) 1159
PsuI RGATCY 1 cut(s) 586
PsyI GACNNNGTC 1 cut(s) 179
RsaI GTAC 2 cut(s) 489, 1194
RsaNI GTAC 2 cut(s) 488, 1193
RseI CAYNNNNRTG 1 cut(s) 933
SaqAI TTAA 4 cut(s) 21, 297, 833, 867
SatI GCNGC 1 cut(s) 1058
Sau3AI GATC 9 cut(s) 265, 307, 415, 586, 694, 701, 1008, 1063, 1120
Sau96I GGNCC 1 cut(s) 1159
ScaI AGTACT 2 cut(s) 489, 1194
ScrFI CCNGG 1 cut(s) 1163
SfaNI GCATC 1 cut(s) 623
SfuI TTCGAA 2 cut(s) 723, 1223
SinI GGWCC 1 cut(s) 1159
SmiMI CAYNNNNRTG 1 cut(s) 933
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 8 cut(s) 93, 167, 747, 794, 868, 882, 981, 1209
SsiI CCGC 1 cut(s) 1058
SspI AATATT 1 cut(s) 1220
SspMI CTAG 5 cut(s) 381, 435, 654, 1140, 1307
StyD4I CCNGG 1 cut(s) 1161
StyI CCWWGG 2 cut(s) 636, 956
TaaI ACNGT 3 cut(s) 181, 441, 1146
TaiI ACGT 2 cut(s) 1053, 1230
TaqI TCGA 4 cut(s) 723, 737, 851, 1223
TasI AATT 8 cut(s) 93, 167, 747, 794, 868, 882, 981, 1209
TatI WGTACW 2 cut(s) 487, 1192
TauI GCSGC 1 cut(s) 1060
TfiI GAWTC 6 cut(s) 85, 427, 809, 853, 925, 1132
Tru1I TTAA 4 cut(s) 21, 297, 833, 867
Tru9I TTAA 4 cut(s) 21, 297, 833, 867
TscAI CASTG 2 cut(s) 184, 1299
TseFI GTSAC 2 cut(s) 466, 1171
Tsp45I GTSAC 2 cut(s) 466, 1171
TspDTI ATGAA 1 cut(s) 519
TspRI CASTG 2 cut(s) 184, 1299
Tth111I GACNNNGTC 1 cut(s) 179
Van91I CCANNNNNTGG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 1159
VspI ATTAAT 1 cut(s) 867
XapI RAATTY 2 cut(s) 167, 1209
XbaI TCTAGA 1 cut(s) 434
XceI RCATGY 1 cut(s) 1046
XcmI CCANNNNNNNNNTGG 1 cut(s) 20
XmiI GTMKAC 1 cut(s) 803
XmnI GAANNNNTTC 2 cut(s) 84, 207
XspI CTAG 5 cut(s) 381, 435, 654, 1140, 1307
ZrmI AGTACT 2 cut(s) 489, 1194
Zsp2I ATGCAT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.